Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,816,983 A→G 5.3% R51R (CGT→CGC)  chbG ← chito‑oligosaccharide deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,816,9830AG 5.3% 232.6 / 2.9 75R51R (CGT→CGC) chbGchito‑oligosaccharide deacetylase
Reads supporting (aligned to +/- strand):  ref base A (38/33);  new base G (2/2);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

TAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTT  >  NC_000913/1816835‑1817131
                                                                                                                                                    |                                                                                                                                                    
tAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTtcatca                                                                                                                                                      <  8:101399/149‑1 (MQ=255)
   tttCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGa                                                                                                                                                   <  4:86439/149‑1 (MQ=255)
   tttCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGa                                                                                                                                                   <  3:28417/149‑1 (MQ=255)
    ttcttcCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCGCGAc                                                                                                                                                  >  3:259721/1‑149 (MQ=255)
          cAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCGCGACTCAAc                                                                                                                                             <  4:259721/148‑1 (MQ=255)
          cAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACt                                                                                                                                            >  8:183573/1‑149 (MQ=255)
              ggTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCAc                                                                                                                                        <  8:276280/149‑1 (MQ=255)
               gTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCAcc                                                                                                                                       <  2:386721/149‑1 (MQ=255)
               gTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCAcc                                                                                                                                       >  3:264998/1‑149 (MQ=255)
               gTAAAGCATCTTCTTATGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCAcc                                                                                                                                       <  4:264998/149‑1 (MQ=255)
                tAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCg                                                                                                                                      >  2:184571/1‑149 (MQ=255)
                    gCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCaa                                                                                                                                              <  1:385011/137‑1 (MQ=255)
                    gCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCaa                                                                                                                                              >  2:385011/1‑137 (MQ=255)
                    gCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATg                                                                                                                                  <  2:134165/149‑1 (MQ=255)
                    gCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATg                                                                                                                                  >  6:249807/1‑149 (MQ=255)
                    gCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATg                                                                                                                                  <  7:242753/149‑1 (MQ=255)
                    gCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCAGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATg                                                                                                                                  <  8:196543/149‑1 (MQ=255)
                     cATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATgg                                                                                                                                 <  7:207155/149‑1 (MQ=255)
                        cttcttctGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCGCGACTCAACTGCACCGCATGGTc                                                                                                                               >  1:54336/1‑148 (MQ=255)
                         ttcttctGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCaa                                                                                                                             >  1:10638/1‑149 (MQ=255)
                                 ccAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCaa                                                                                                                             <  6:245693/141‑1 (MQ=255)
                                 ccAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCaa                                                                                                                             >  5:245693/1‑141 (MQ=255)
                                  cAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGcc                                                                                                                    <  7:391946/149‑1 (MQ=255)
                                  cAACTGCCAGATCCATTTTCCCAGCACACCATCGCAGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGcc                                                                                                                    >  5:142639/1‑149 (MQ=255)
                                   aaCTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGccc                                                                                                                   <  2:10638/149‑1 (MQ=255)
                                     cTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCAt                                                                                                                 >  7:315093/1‑149 (MQ=255)
                                      tGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCAtt                                                                                                                <  2:118190/149‑1 (MQ=255)
                                      tGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATAACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCAtt                                                                                                                >  7:165422/1‑149 (MQ=255)
                                        ccAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCa                                                                                                              >  4:159896/1‑149 (MQ=255)
                                        ccAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCa                                                                                                              >  4:229476/1‑149 (MQ=255)
                                         cAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCGCGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCAc                                                                                                             <  2:54336/149‑1 (MQ=255)
                                          agctCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCAc                                                                                                             >  8:368544/4‑148 (MQ=255)
                                          aGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCAc                                                                                                             >  5:112038/1‑148 (MQ=255)
                                               cATTTTCCCAGCACACCATCGCGGGTTAACCCCGGAATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGAACCGCATGGTCAATAGCCTGCCCAATCACAAgcgc                                                                                                       >  1:287110/1‑149 (MQ=255)
                                                 ttttCCCAGCACGCCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCcg                                                                                                     >  5:361631/1‑149 (MQ=255)
                                                      ccAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCgtcgtc                                                                                                <  7:368544/149‑1 (MQ=255)
                                                         gCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGAc                                                                                             <  8:293234/149‑1 (MQ=255)
                                                             acCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGa                                                                                         <  3:159896/149‑1 (MQ=255)
                                                                 tCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAAt                                                                                     >  8:167266/1‑149 (MQ=255)
                                                                  cgcgGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATc                                                                                    >  5:210528/1‑149 (MQ=255)
                                                                             cccGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACa                                                                         <  7:183573/149‑1 (MQ=255)
                                                                                 gCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGTCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGcc                                                                     <  8:193142/149‑1 (MQ=255)
                                                                                    tAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCaa                                                                                                                                              >  2:57795/1‑73 (MQ=255)
                                                                                      gCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGAt                                                                <  6:142639/149‑1 (MQ=255)
                                                                                      gCTGTCAGTGGCGTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGa                                                                 <  6:141280/148‑1 (MQ=255)
                                                                                          tCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATaa                                                              <  6:361631/147‑1 (MQ=255)
                                                                                          tCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATg                                                            >  7:137994/1‑149 (MQ=255)
                                                                                                   tACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGa                                                                 <  5:284284/135‑1 (MQ=255)
                                                                                                   tACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGa                                                                 >  2:267776/1‑135 (MQ=255)
                                                                                                   tACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGa                                                                 >  6:284284/1‑135 (MQ=255)
                                                                                                   tACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGa                                                                 <  1:267776/135‑1 (MQ=255)
                                                                                                   tACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTc                                                   >  3:161112/1‑149 (MQ=255)
                                                                                                    aCCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCt                                                  >  2:281388/1‑149 (MQ=255)
                                                                                                         tAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCt                                             >  3:382888/1‑149 (MQ=255)
                                                                                                           gTAAGGACAAAGTTCATCCCTATGGCCAGACTAGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCAGCCCATACACCAGCGCAGTCGTCGACGTGACAATCCCATTGCGCCCGGCCTCGATAATGACGTACTTCTGACCttt                                           >  2:204493/1‑149 (MQ=255)
                                                                                                           gTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCttt                                           <  3:229476/149‑1 (MQ=255)
                                                                                                                gACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCtt                                       >  7:53045/1‑148 (MQ=255)
                                                                                                                   aaaGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGc                                   >  2:273701/1‑149 (MQ=255)
                                                                                                                    aaGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGcc                                  <  6:112038/149‑1 (MQ=255)
                                                                                                                         cATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCaaaa                              <  5:249807/148‑1 (MQ=255)
                                                                                                                         cATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAat                             >  6:266709/1‑149 (MQ=255)
                                                                                                                           tCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAatca                           >  1:289434/1‑149 (MQ=255)
                                                                                                                             ccTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAatcatc                         <  1:184571/149‑1 (MQ=255)
                                                                                                                              cTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCg                        <  6:210528/149‑1 (MQ=255)
                                                                                                                               tATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCcgtcgt                                                                                                 <  4:20609/75‑1 (MQ=255)
                                                                                                                               tATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCcgtcgt                                                                                                 >  3:20609/1‑75 (MQ=255)
                                                                                                                                 tGGCCAGACTCGGTTCATCACGACTCAACTGCACCGAATGGTCAATAGACTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCgg                       <  2:287110/147‑1 (MQ=255)
                                                                                                                                    ccAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTc                                                                   >  4:144795/1‑100 (MQ=255)
                                                                                                                                    ccAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTc                                                                   <  3:144795/100‑1 (MQ=255)
                                                                                                                                     cAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTaa                 <  1:281388/149‑1 (MQ=255)
                                                                                                                                      aGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTaa                 >  6:134173/1‑148 (MQ=255)
                                                                                                                                        aCTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACaa              >  4:110577/1‑149 (MQ=255)
                                                                                                                                          tCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAAt             >  6:70883/1‑148 (MQ=255)
                                                                                                                                          tCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGGGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAAt             >  3:89760/1‑148 (MQ=255)
                                                                                                                                              ttCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGt         >  8:317361/1‑148 (MQ=255)
                                                                                                                                              ttCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGt         >  8:366642/1‑148 (MQ=255)
                                                                                                                                                 atcaCGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAg      <  8:315093/148‑1 (MQ=255)
                                                                                                                                                  tcaCGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCg    <  5:134173/149‑1 (MQ=255)
                                                                                                                                                   caCGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGt   >  7:17926/1‑149 (MQ=255)
                                                                                                                                                    aCGACTCAACTGAACAGCATGGTCAATAGCCTGCCCATTCACAAGCGCCGTCGTCGACGTGACAATACCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAAGCAGTAAGCGtt  >  4:378143/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTT  >  NC_000913/1816835‑1817131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑