Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 262,225 G→T 8.7% V241V (GTG→GTT proA → gamma‑glutamylphosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913262,2250GT 8.7% 295.2 / 2.9 97V241V (GTG→GTTproAgamma‑glutamylphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (43/44);  new base T (8/2);  total (51/46)
Fisher's exact test for biased strand distribution p-value = 9.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCA  >  NC_000913/262078‑262372
                                                                                                                                                   |                                                                                                                                                   
aTACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGa                                                                                                                                                    <  8:175388/149‑1 (MQ=255)
  aCATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATTCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATc                                                                                                                                                  >  5:356187/1‑149 (MQ=255)
   cATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATc                                                                                                                                                  <  7:409712/148‑1 (MQ=255)
   cATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCACGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACTTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATc                                                                                                                                                  <  3:311990/148‑1 (MQ=255)
    aTCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGt                                                                                                                                                >  2:83080/1‑149 (MQ=255)
     tCGACATGCTGATCCCGCGTGGTGGCGCTTGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTc                                                                                                                                               <  8:64544/149‑1 (MQ=255)
         cATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAAcg                                                                                                                                           <  5:198872/149‑1 (MQ=255)
                aTCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTAAACGCGAAAAc                                                                                                                                    >  6:354946/1‑149 (MQ=255)
                aTCCCGCGTGGTGGCGCGGGTTTGCATAAACTGTGCCGGGACCAGTCGCCCATCCCGGTGCTCACAGGTGGTATTGGCGTAGGCCATATTTACGTTGATGAAAGTGTACAGCTCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAAc                                                                                                                                    <  2:313438/149‑1 (MQ=255)
                   ccGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTc                                                                                                                                  <  1:83080/148‑1 (MQ=255)
                   ccGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCa                                                                                                                                 <  4:79626/149‑1 (MQ=255)
                     gcgTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGGGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGc                                                                                                                               <  6:46769/149‑1 (MQ=255)
                           tggCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTcc                                                                                                                           >  1:77647/1‑147 (MQ=255)
                             gggcTGTTTTGCATATACTGTGCCTTTAACAGTCGACAATCCCGGTGATCACAGGTGTTTTAGGCGTATGACATATTTACGTTGATGAATGTGTAGAGATCGCTGAAGCATTAAAAGTTATCGTCAACGCGAAAACTCAGCGTCCGAg                                                                                                                        <  2:19815/146‑1 (MQ=255)
                               gcTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGcac                                                                                                                     <  8:111972/149‑1 (MQ=255)
                               gcTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGca                                                                                                                      >  1:333251/1‑148 (MQ=255)
                               gcTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGca                                                                                                                      >  2:306373/1‑148 (MQ=255)
                                  ggTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATg                                                                                                                  <  4:60446/149‑1 (MQ=255)
                                   gTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGCTGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGt                                                                                                                 <  2:279412/149‑1 (MQ=255)
                                           aaaCTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGt                                                                                                         >  1:146856/1‑149 (MQ=255)
                                                 tgCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATATGCGTATGCCATATTTACGTTGATTAAAGTGTAGAGATCGCTTAAGCATTAAAAGTTATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACTGTTGAAAc                                                                                                   <  5:355552/149‑1 (MQ=255)
                                                     gTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTg                                                                                               >  1:364644/1‑149 (MQ=255)
                                                      tGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTTATCGTCAACGCGAAACCTCAGCGTCCGAGCACATGTAATACGGGTGAAACGTTGc                                                                                              >  6:214117/1‑149 (MQ=255)
                                                      tGAACAGTCGACAATCCAGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGc                                                                                              <  6:356187/149‑1 (MQ=255)
                                                         aCAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCa                                                                                                                                 <  3:344436/111‑1 (MQ=255)
                                                         aCAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCa                                                                                                                                 >  4:344436/1‑111 (MQ=255)
                                                          cAGTCGACAATCCCGGTGATCACAGGGGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGt                                                                                          <  4:324670/149‑1 (MQ=255)
                                                           aGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTACTGGt                                                                                          >  3:262179/1‑148 (MQ=255)
                                                             tCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGaa                                                                                       >  7:72424/1‑149 (MQ=255)
                                                             tCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCAAATGTAATACGGTTGAAACGTTGCTGGTGaa                                                                                       >  3:245374/1‑149 (MQ=255)
                                                               gACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAAt                                                                                      <  2:71112/148‑1 (MQ=255)
                                                               gACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAAt                                                                                      >  3:301604/1‑148 (MQ=255)
                                                               gACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATAGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGGTGATGGTGAAt                                                                                      >  7:149434/1‑148 (MQ=255)
                                                                aCAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATaa                                                                                    <  8:72424/149‑1 (MQ=255)
                                                                aCAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATaa                                                                                    <  1:16614/149‑1 (MQ=255)
                                                                  aaTCCCGGTGATCACAGGTGGTATAGCCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCAGGTGAATaaa                                                                                   >  7:349598/1‑148 (MQ=255)
                                                                   aTCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACTTTGCTGGTGAATaaaaa                                                                                 <  2:333251/149‑1 (MQ=255)
                                                                    tCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAATCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACAAGTAATACGGAGGAAACGTTGCTGGAGAATaaaaa                                                                                 >  7:157511/1‑148 (MQ=255)
                                                                        ggTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCg                                                                            >  8:175420/1‑149 (MQ=255)
                                                                        ggTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATATTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATAGGGTTGAAACGTTGCTGGTGAATAAAAACATCg                                                                            >  6:12134/1‑149 (MQ=255)
                                                                          tGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGATATCGCTGAACCAATAAAAATTAAAAAAAACTCGAAAACTCAGCGTCCGAGCACTTGTAATACGGTTGAAACGTTGCTGGTGAATATAAACATCTcc                                                                          >  6:217864/1‑149 (MQ=255)
                                                                            aTCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGa                                                                        <  2:146856/149‑1 (MQ=255)
                                                                            aTCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGa                                                                        <  7:326935/149‑1 (MQ=255)
                                                                            aTCACAGGTGGGATAGGCGTATGCAATATTAACGTTGATGATTGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGTATAAAAACATCGCCGa                                                                        <  8:349598/149‑1 (MQ=255)
                                                                             tCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGa                                                                        <  7:228300/148‑1 (MQ=255)
                                                                             tCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGAt                                                                       <  8:350896/149‑1 (MQ=255)
                                                                             tCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCAGTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGa                                                                        >  3:311782/1‑148 (MQ=255)
                                                                                   gtggtATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGATATCGCTGAACCAATAAAAATTAAAAAAAACTAGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTc                                                                 >  8:43702/1‑149 (MQ=255)
                                                                                   gtggtATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTTATCATCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTc                                                                 >  2:172931/1‑149 (MQ=255)
                                                                                    tggtATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGATATCGCTGCACCAATAAAAATTATCATCAAATCGAAAACTCAGCGTCCGGGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTcc                                                                >  6:103967/1‑149 (MQ=255)
                                                                                    tggtATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTTATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTcc                                                                <  8:90464/149‑1 (MQ=255)
                                                                                       tataGGCGTATGCCATATTTACGTTGATGAAAGTGTAGATATCGCTGAACCAATAAAAATTAAAAAAAAAACGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGc                                                             >  4:150491/1‑149 (MQ=255)
                                                                                       tataGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGc                                                             >  8:217717/1‑149 (MQ=255)
                                                                                         taGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGccc                                                           >  4:69297/1‑149 (MQ=255)
                                                                                           ggCGTATGCCATATTTACGTTGATGAAAGTGTAGAGCTCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCAGATGTAATAGGGGTGAAAGGTTGCTGGTGATTAAAATGATCGCGGATAGCTTCCTGCCCGc                                                         >  4:50482/1‑149 (MQ=255)
                                                                                               tATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTa                                                                                                                >  8:248334/1‑90 (MQ=255)
                                                                                               tATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTa                                                                                                                <  7:248334/90‑1 (MQ=255)
                                                                                                 tGCCATATTTACGTTGATGAAAGTGTAGATATCGCTGAACCATTAAAAATTATATTAAACTCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACCTCGCCGATAGCTTCCTGCCCGCATTAAg                                                   >  4:339012/1‑149 (MQ=255)
                                                                                                 tGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATATTAAACGCGAAAACTCAGCGTCCGAGCACATGTAATAGGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAg                                                   >  4:83971/1‑149 (MQ=255)
                                                                                                   ccATATTTACGTTGATGAAAGTGTAGATATCGCTGAACCAATAAAAATTAAAAAAAAAAAGAAAACTCAGCGTCCGAGCACATGTAAGAGGGTTGAAACGTTGCTGGTGAATAAAAACTTCGCCGATAGCTTCCTGACCGCAtttcgc                                                  >  4:157287/1‑144 (MQ=255)
                                                                                                   ccATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGca                                                 <  5:354946/149‑1 (MQ=255)
                                                                                                     atatTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTTCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGcaaa                                               <  4:262179/149‑1 (MQ=255)
                                                                                                      tatTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGcaaac                                              <  6:390276/149‑1 (MQ=255)
                                                                                                      tatTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGcaaa                                               >  7:198844/1‑148 (MQ=255)
                                                                                                          tACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGcaaacaaa                                           <  2:77647/148‑1 (MQ=255)
                                                                                                             gTTGATGAAAGTTTAGAGATCGCTGAAGCATTAAAAGTGATCGTAAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGc                                       >  8:19926/1‑149 (MQ=255)
                                                                                                             gTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGc                                       >  2:267028/1‑149 (MQ=255)
                                                                                                             gTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGc                                       <  7:175420/149‑1 (MQ=255)
                                                                                                               tgatgaAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCg                                      >  8:340106/1‑148 (MQ=255)
                                                                                                                gatgaAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCgg                                     >  3:293678/1‑148 (MQ=255)
                                                                                                                  tgaAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGa                                    >  7:19191/1‑147 (MQ=255)
                                                                                                                    aaaGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAgc                                >  2:137007/1‑149 (MQ=255)
                                                                                                                    aaaGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCCATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAgc                                <  3:50482/149‑1 (MQ=255)
                                                                                                                     aaGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAgcg                               >  7:119441/1‑149 (MQ=255)
                                                                                                                     aaGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAgcg                               >  1:148798/1‑149 (MQ=255)
                                                                                                                       gtgtAGTTATCGCTGAAGCATAAAAAGTGTTCGTCAACGCGTAATGTCAGCGTACTAGCTCATGTAATACGGTGGAATCGTTGCTGGTGAATATTAACTTCGCCGTTAGCTTCCTGCCCGCATTAAGCAAAGAAATGGCGGAAAgcgtc                             <  8:157511/149‑3 (MQ=255)
                                                                                                                        tgtAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAgcggcg                            >  3:131882/1‑149 (MQ=255)
                                                                                                                         gtAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAgcggcg                            <  2:148798/148‑1 (MQ=255)
                                                                                                                           agagATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGa                         >  1:137269/1‑149 (MQ=255)
                                                                                                                               aTCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAACCATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACAt                      >  1:291445/1‑148 (MQ=255)
                                                                                                                               aTCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTAAGAGTCAGAGCACATGTAAGAAGGTTGAAACTTTGATCGTGAATAAAAACATCGCCGATATCTTCATGACCGCATTAAGAAAACAAATGGAGGATAGCGGCGTGACAt                      >  7:281806/1‑148 (MQ=255)
                                                                                                                                 cGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTa                    >  6:277169/1‑148 (MQ=255)
                                                                                                                                      aaGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAAc                                                                                                   <  2:287584/64‑1 (MQ=255)
                                                                                                                                      aaGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAAc                                                                                                   >  1:287584/1‑64 (MQ=255)
                                                                                                                                      aaGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGc               <  1:267028/148‑1 (MQ=255)
                                                                                                                                       aGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAg             <  5:217864/149‑1 (MQ=255)
                                                                                                                                          aTTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATg          >  4:142042/1‑149 (MQ=255)
                                                                                                                                           ttAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATg          >  7:226327/1‑148 (MQ=255)
                                                                                                                                              aaaGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAATCGGCGTGACATTACACGCAGATGCAg       <  8:198844/148‑1 (MQ=255)
                                                                                                                                              aaaGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAg       <  4:131882/148‑1 (MQ=255)
                                                                                                                                               aaGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCt     <  3:69297/149‑1 (MQ=255)
                                                                                                                                                 gTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCa                                                        <  3:134071/96‑1 (MQ=255)
                                                                                                                                                 gTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCa                                                        >  4:134071/1‑96 (MQ=255)
                                                                                                                                                 gTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGc   <  2:364644/149‑1 (MQ=255)
                                                                                                                                                 gTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGAATTAAGCAAACAAATGGAGGAAAGCGGCGTGAAATTACACGCAGATGAAGCTg    >  1:189304/1‑148 (MQ=255)
                                                                                                                                                  tGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCa  <  8:226327/149‑1 (MQ=255)
                                                                                                                                                  tGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCa  <  7:217717/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTGGTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGCGGCGTGACATTACACGCAGATGCAGCTGCA  >  NC_000913/262078‑262372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: