Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,047,453 C→T 5.0% T839I (ACT→ATT)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,047,4530CT 5.0% 263.5 / 2.5 79T839I (ACT→ATT) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base C (37/38);  new base T (2/2);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

ACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATGTTAATTCAGCAGAGGCGAAA  >  NC_000913/2047306‑2047601
                                                                                                                                                   |                                                                                                                                                    
acaaTCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACt                                                                                                                                                     <  7:165594/149‑1 (MQ=255)
    tCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTgcg                                                                                                                                                  >  7:142180/1‑148 (MQ=255)
      aaaaCACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAg                                                                                                                                               >  7:141560/1‑149 (MQ=255)
            ccGCAAACACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTACGCACGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGGCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGAt                                                                                                                                         <  2:281917/149‑1 (MQ=255)
            ccGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACTCGGTTGAAGTCACCCTTGAAAATGGCGTGATACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGAt                                                                                                                                         <  6:82931/149‑1 (MQ=255)
            ccGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGAt                                                                                                                                         <  4:141986/149‑1 (MQ=255)
            ccGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGAt                                                                                                                                         <  3:173587/149‑1 (MQ=255)
            ccGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGAt                                                                                                                                         <  7:297805/149‑1 (MQ=255)
                  aaaCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGct                                                                                                                                    >  2:43896/1‑147 (MQ=255)
                  aaaCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAg                                                                                                                                   <  3:162341/149‑1 (MQ=255)
                    aCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGaa                                                                                                                                 <  4:314489/149‑1 (MQ=255)
                         tGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGa                                                                                                                            <  3:35287/149‑1 (MQ=255)
                              aTGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAAt                                                                                                                       >  4:238818/1‑149 (MQ=255)
                               tGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATg                                                                                                                      <  4:172966/149‑1 (MQ=255)
                                      gCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTg                                                                                                                 <  2:311638/147‑1 (MQ=255)
                                      gCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGt                                                                                                               <  8:386521/149‑1 (MQ=255)
                                               gATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACg                                                                                                       <  1:365849/148‑1 (MQ=255)
                                                aTCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACgg                                                                                                      >  8:263165/1‑148 (MQ=255)
                                                 tCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTTAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGaa                                                                                                                                 >  8:53203/1‑120 (MQ=255)
                                                 tCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTTAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGaa                                                                                                                                 <  7:53203/120‑1 (MQ=255)
                                                 tCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGc                                                                                                     >  8:117800/1‑148 (MQ=255)
                                                   tGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAAt                                                                                                  >  3:174113/1‑149 (MQ=255)
                                                    gAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGc                                                                                                                                     <  2:361008/113‑1 (MQ=255)
                                                    gAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAAt                                                                                                  >  7:90949/1‑148 (MQ=255)
                                                    gAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAAt                                                                                                  >  5:244900/1‑148 (MQ=255)
                                                     aaaaGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAAt                                                                                                  <  6:370641/147‑1 (MQ=255)
                                                     aaaaGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGa                                                                                                >  8:54617/1‑149 (MQ=255)
                                                     aaaaGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGa                                                                                                <  6:96827/149‑1 (MQ=255)
                                                     aaaaGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTCAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCaa                                                                                                   <  2:318578/146‑1 (MQ=255)
                                                      aaaGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGAc                                                                                               <  3:348377/149‑1 (MQ=255)
                                                        agtagtAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAg                                                                                             <  7:263165/149‑1 (MQ=255)
                                                             taAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTca                                                                                        >  7:293763/1‑149 (MQ=255)
                                                                 cAGGAAGACAACACGTTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAAt                                                                                    <  3:204689/149‑1 (MQ=255)
                                                                  aGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAAt                                                                                    <  6:244900/148‑1 (MQ=255)
                                                                    gAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGAc                                                                                 >  8:351405/1‑149 (MQ=255)
                                                                       gACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACcgc                                                                              >  3:239644/1‑149 (MQ=255)
                                                                          aacaCGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGAc                                                                           >  2:173734/1‑149 (MQ=255)
                                                                           acacGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGAcc                                                                          >  8:3862/1‑149 (MQ=255)
                                                                            cacGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCg                                                                         >  8:49228/1‑149 (MQ=255)
                                                                            cacGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCg                                                                         >  2:169386/1‑149 (MQ=255)
                                                                            cacGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCg                                                                         >  2:369543/1‑149 (MQ=255)
                                                                            cacGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAAACGGCAGTTATGTCGGCGCCTAGAGTCCTGCGCATGTTGATCTGCAGAAGTCGAAAAATGTCGTGGATGCTGACGGCAATGACCGCGTCCCAATGCGCTCGACCg                                                                         >  6:366796/1‑149 (MQ=255)
                                                                               ggTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTcc                                                                      >  6:320215/1‑149 (MQ=255)
                                                                                     aGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATg                                                                <  1:169386/149‑1 (MQ=255)
                                                                                      gTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGc                                                               <  4:106853/149‑1 (MQ=255)
                                                                                       tCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTATTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGc                                                               >  7:34050/1‑148 (MQ=255)
                                                                                       tCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGc                                                               >  5:126741/1‑148 (MQ=255)
                                                                                       tCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGc                                                               >  7:359010/1‑148 (MQ=255)
                                                                                       tCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGc                                                               >  7:302752/1‑148 (MQ=255)
                                                                                       tCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCa                                                              <  6:204820/149‑1 (MQ=255)
                                                                                       tCACCATTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTATTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACTGCAATGCCAGCGTCACAATGACCGCGACCGTCCGGGATGc                                                               >  7:27855/1‑148 (MQ=255)
                                                                                          cccTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCaaaa                                                           <  7:117800/149‑1 (MQ=255)
                                                                                           ccTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTg                                                                                                                                      >  3:103306/1‑73 (MQ=255)
                                                                                           ccTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTg                                                                                                                                      <  4:103306/73‑1 (MQ=255)
                                                                                           ccTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGATAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAg                                                          <  3:238818/149‑1 (MQ=255)
                                                                                           ccTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAg                                                          >  7:213825/1‑149 (MQ=255)
                                                                                                 aaaTGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAAcc                                                    >  2:77965/1‑149 (MQ=255)
                                                                                                 aaaTGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAAcc                                                    >  3:335038/1‑149 (MQ=255)
                                                                                                            aacaaacGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGAc                                         <  8:213825/149‑1 (MQ=255)
                                                                                                               aaacGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGt                                       >  3:96933/1‑148 (MQ=255)
                                                                                                                      aaTCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTc                                <  5:320215/148‑1 (MQ=255)
                                                                                                                       aTCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCAc                              >  6:166267/1‑149 (MQ=255)
                                                                                                                          gTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACttt                           >  6:183239/1‑149 (MQ=255)
                                                                                                                          gTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACttt                           >  1:231613/1‑149 (MQ=255)
                                                                                                                             aGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCaa                        <  4:239644/149‑1 (MQ=255)
                                                                                                                              gTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATTAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAAt                       <  8:379760/149‑1 (MQ=255)
                                                                                                                              gTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAAt                       <  4:174113/149‑1 (MQ=255)
                                                                                                                               ttttGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATg                      >  1:46473/1‑149 (MQ=255)
                                                                                                                                tttGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATg                      <  7:54617/148‑1 (MQ=255)
                                                                                                                                     cGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATGTTAAtt                >  5:23018/1‑149 (MQ=255)
                                                                                                                                      ggCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATGTTAAtt                <  8:142180/148‑1 (MQ=255)
                                                                                                                                          aCTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATGTTAATTcagca           <  4:182613/149‑1 (MQ=255)
                                                                                                                                              gAGTATTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATGTTAATTCAGCaga         <  8:27855/147‑1 (MQ=255)
                                                                                                                                              gAGTATTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGGCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATGTTAATTCAGCaga         <  8:34050/147‑1 (MQ=255)
                                                                                                                                              gAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCTTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATGTTAATTCAGCAGAgg       <  1:43896/149‑1 (MQ=255)
                                                                                                                                              gAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATGTTAATTCAGCagag        <  1:369543/148‑1 (MQ=255)
                                                                                                                                                 tACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATGTTAATTCAGCAGAGGCg     >  1:197800/1‑148 (MQ=255)
                                                                                                                                                 tACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATGTTAATTCAGCAGAGGCg     >  6:339319/1‑148 (MQ=255)
                                                                                                                                                 tACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATGTTAATTCAGCAGAGGCg     >  8:335735/1‑148 (MQ=255)
                                                                                                                                                   cTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGGTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATGTTAATTCAGCAGAGGCGaaa  <  8:141560/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTTTCAATGTTAATTCAGCAGAGGCGAAA  >  NC_000913/2047306‑2047601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑