Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,485,594	G→A	5.0%	R75R (CGC→CGT)	yhfA ←	OsmC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,485,594	0	G	A	5.0%	261.5 / 2.0	79	R75R (CGC→CGT)	yhfA	OsmC family protein
Reads supporting (aligned to +/- strand):  ref base G (35/40);  new base A (2/2);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTT  >  NC_000913/3485446‑3485737                                                                                                                                                     |                                                                                                                                                tatTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTcg                                                                                                                                                 >  5:117338/1‑149 (MQ=255)     cACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCgcgg                                                                                                                                              <  7:40216/148‑1 (MQ=255)      aCCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCgcggcg                                                                                                                                            >  3:12467/1‑149 (MQ=255)      aCCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCgcggcg                                                                                                                                            >  4:185272/1‑149 (MQ=255)       ccGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGt                                                                                                                                           >  8:234623/1‑149 (MQ=255)       ccGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGt                                                                                                                                           <  8:23405/149‑1 (MQ=255)          cTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCa                                                                                                                                        <  5:366758/149‑1 (MQ=255)          cTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCa                                                                                                                                        <  2:184197/149‑1 (MQ=255)          cTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCa                                                                                                                                        <  2:164112/149‑1 (MQ=255)              ttCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCagag                                                                                                                                     <  3:239262/148‑1 (MQ=255)              atCCAGCCGCAACGCCACTGACCAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGGCGCGACCGGGGACGATAAAATGCAGATTAATGTGCGTACACAGGCGCGGTGCCTCTTCGCGGCGTTCagag                                                                                                                                     <  6:371011/147‑1 (MQ=255)                   gCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAAt                                                                                                                               <  4:12708/149‑1 (MQ=255)                   gCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAAt                                                                                                                               <  1:155219/149‑1 (MQ=255)                    cATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAAtt                                                                                                                              >  8:356982/1‑149 (MQ=255)                      tCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAAttt                                                                                                                             >  7:288541/1‑148 (MQ=255)                      tCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAAttt                                                                                                                             >  4:265869/1‑148 (MQ=255)                      tCAAAGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGAAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAAtgt                                                                                                                             >  6:157923/1‑146 (MQ=255)                        aaCGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTAc                                                                                                                          >  6:42943/1‑149 (MQ=255)                        aaCGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTAc                                                                                                                          >  2:393409/1‑149 (MQ=255)                          cGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACtt                                                                                                                        <  7:356982/149‑1 (MQ=255)                           gCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTc                                                                                                                       <  3:10199/149‑1 (MQ=255)                             cACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCACGGCGTTCAGAGGTCAATTTTACTTca                                                                                                                      >  2:240225/1‑148 (MQ=255)                             cACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGAAAACAGGCGTGGTGCCTCATAACTGCTTTAAGAGGTCAATTTTagatca                                                                                                                      >  2:240226/1‑143 (MQ=255)                                  aaCAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATc                                                                                                                 <  7:234623/148‑1 (MQ=255)                                    cAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGAc                                                                                                              <  4:120717/149‑1 (MQ=255)                                     aaTATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGAAGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGAc                                                                                                              >  6:376586/1‑148 (MQ=255)                                       tatTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACca                                                                                                            >  6:266121/1‑148 (MQ=255)                                         tttCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACAt                                                                                                         >  5:206659/1‑149 (MQ=255)                                                      agaTCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCttt                                                                                            >  2:309694/1‑149 (MQ=255)                                                        aTCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCt                                                                                              <  8:367895/145‑1 (MQ=255)                                                        aTCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCt                                                                                              >  7:367895/1‑145 (MQ=255)                                                         tCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCTACAGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGCCCACATCCTGACGCGCGTTTTg                                                                                         >  4:62218/1‑149 (MQ=255)                                                           aaCCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCa                                                                                       <  8:288541/149‑1 (MQ=255)                                                              cGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGCCCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGa                                                                                    >  5:240048/1‑149 (MQ=255)                                                               gCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGAt                                                                                   <  6:117338/149‑1 (MQ=255)                                                                cACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGa                                                                                                               >  5:56981/1‑120 (MQ=255)                                                                cACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGa                                                                                                               <  6:56981/120‑1 (MQ=255)                                                                cACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATc                                                                                  <  8:344511/149‑1 (MQ=255)                                                                     gcAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGAc                                                                                                              <  2:408841/116‑1 (MQ=255)                                                                     gcAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGAc                                                                                                              >  1:408841/1‑116 (MQ=255)                                                                       aaCCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATc                                                                                                        <  2:157979/120‑1 (MQ=255)                                                                        aCCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACca                                                                           >  5:245184/1‑148 (MQ=255)                                                                         ccGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTAc                                                                                                                          <  8:131249/100‑1 (MQ=255)                                                                         ccGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTAc                                                                                                                          >  7:131249/1‑100 (MQ=255)                                                                          cGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACAt                                                                        <  4:12467/149‑1 (MQ=255)                                                                           gCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATc                                                                       <  7:48115/149‑1 (MQ=255)                                                                            cTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATc                                                                       >  7:54704/1‑148 (MQ=255)                                                                             tGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGa                                                                     >  2:44897/1‑149 (MQ=255)                                                                             tGCGTCTTTCAGGTCGCGACCGGGGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCGCAATCGACCACATCCTGCCGCCCTGTTTGCAGGATCGAAACCACCTCGa                                                                     >  2:82810/1‑149 (MQ=255)                                                                               cGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGAt                                                                    <  3:265869/148‑1 (MQ=255)                                                                                     tCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACt                                                              <  6:206659/148‑1 (MQ=255)                                                                                       aGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATc                                                                                                        >  3:19322/1‑104 (MQ=255)                                                                                       aGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATc                                                                                                        <  4:19322/104‑1 (MQ=255)                                                                                        ggTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCAACCACATCCTGa                                                                                                    >  2:220432/1‑107 (MQ=255)                                                                                        ggTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCAACCACATCCTGa                                                                                                    <  1:220432/107‑1 (MQ=255)                                                                                              gACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATc                                                                                                        >  2:142087/1‑97 (MQ=255)                                                                                              gACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATc                                                                                                        <  1:142087/97‑1 (MQ=255)                                                                                                    tGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCgccgcc                                              <  3:134799/149‑1 (MQ=255)                                                                                                    tGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTGCACAATCGACCACATCCGGCCGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCACCCACCcgccgc                                               >  6:43682/1‑148 (MQ=255)                                                                                                      aCGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCAt                                            <  5:266121/149‑1 (MQ=255)                                                                                                         aTAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGAGGGCACTGCAACCACCCGCCGCCATCaa                                         <  5:376586/149‑1 (MQ=255)                                                                                                              aTGCAGATTAATGTGCGTAAACACGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCa                                    <  5:46812/149‑1 (MQ=255)                                                                                                                 cAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCACGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCAttt                                 <  1:240225/149‑1 (MQ=255)                                                                                                                 cAGATTAATGTGCGTAAACAGGCGTGGGGCCTCTTCACGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCAttt                                 <  1:240226/149‑1 (MQ=255)                                                                                                                  aGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCAAAATCGACCACATCATGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCAATCAACACCATTTc                                >  1:260728/1‑149 (MQ=255)                                                                                                                   gATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTcc                               <  3:55500/149‑1 (MQ=255)                                                                                                                      tAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGTAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATc                            >  7:223328/1‑149 (MQ=255)                                                                                                                       aaTGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATc                            <  5:382093/148‑1 (MQ=255)                                                                                                                          gtgCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGAc                        >  2:105599/1‑149 (MQ=255)                                                                                                                            gCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGGCATCAACACCATTTCCATCGGAGtt                      >  1:697/1‑149 (MQ=255)                                                                                                                             cGTAACCAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCAATGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTg                     <  3:62218/149‑1 (MQ=255)                                                                                                                             cGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTg                     <  1:309694/149‑1 (MQ=255)                                                                                                                             cGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCACTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTg                     <  7:153993/149‑1 (MQ=255)                                                                                                                               tAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCa                                                                >  4:40078/1‑104 (MQ=255)                                                                                                                               tAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCa                                                                <  3:40078/104‑1 (MQ=255)                                                                                                                               tAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGt                   >  8:366353/1‑149 (MQ=255)                                                                                                                                aaaCAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTg                  >  3:123246/1‑149 (MQ=255)                                                                                                                                     ggCGTGTTGCCTCTTCGGGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTa             <  3:221229/149‑1 (MQ=255)                                                                                                                                      gCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACATCACAAGCGCCCACATCCTGACGCCCTTTTTGCAGGATCGAACCCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTAt            <  5:157923/149‑1 (MQ=255)                                                                                                                                            tgCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACCTCCTGACGCCCTTTTTGCAGGATCTAACCCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCt       >  8:302028/1‑148 (MQ=255)                                                                                                                                                tctTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGtt  >  4:116979/1‑149 (MQ=255)                                                                                                                                                  ttCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGt   <  4:273746/146‑1 (MQ=255)                                                                                                                                                  ttCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGt   >  3:273746/1‑146 (MQ=255)                                                                                                                                                     |                                                                                                                                                TATTCACCGCTTTTTCCAGCATCAACGCCACTGAACAATATTTCTCGGCAGAGAGATCAACCGCACGCGCAACCGCTGCGTCTTTCAGGTCGCGACCGGTGACGATAAAATGCAGATTAATGTGCGTAAACAGGCGTGGTGCCTCTTCGCGGCGTTCAGAGGTCAATTTTACTTCACAATCGACCACATCCTGACGCCCTTTTTGCAGGATCGAAACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTT  >  NC_000913/3485446‑3485737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑