Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,261,297	G→T	D193E (GAC→GAA)	prs ←	phosphoribosylpyrophosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,261,297	0	G	T	100.0%	353.7 / NA	99	D193E (GAC→GAA)	prs	phosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (49/50);  total (49/50)

TTAGCACCACGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATAC  >  NC_000913/1261151‑1261440                                                                                                                                                   |                                                                                                                                                ttAGCACCACGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTc                                                                                                                                               >  6:336214/1‑149 (MQ=255)  tAGCACCACGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTc                                                                                                                                               <  5:127368/148‑1 (MQ=255)  tAGCACCACGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTc                                                                                                                                               <  4:494892/148‑1 (MQ=255)  tAGCACCACGTTCATTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTc                                                                                                                                               <  3:303911/148‑1 (MQ=255)    gCACCACGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCgat                                                                                                                                            <  7:350037/149‑1 (MQ=255)    gCACCACGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCga                                                                                                                                             <  4:438450/148‑1 (MQ=255)    gCACCACGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCga                                                                                                                                             <  7:446078/148‑1 (MQ=255)        cacGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGAtt                                                                                                                                        <  1:485163/149‑1 (MQ=255)        cacGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGAtt                                                                                                                                        >  1:89819/1‑149 (MQ=255)         acGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTg                                                                                                                                       >  8:71759/1‑149 (MQ=255)          cGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGc                                                                                                                                      <  7:176543/149‑1 (MQ=255)               tttCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCgatga                                                                                                                                          <  1:487442/140‑1 (MQ=255)               tttCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCgatga                                                                                                                                          >  2:487442/1‑140 (MQ=255)                 tCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATc                                                                                                                                <  2:89819/148‑1 (MQ=255)                   agagCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGt                                                                                                                             <  3:504200/149‑1 (MQ=255)                            gcagcTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTcag                                                                                                                    >  2:366354/1‑149 (MQ=255)                             cagcTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTcag                                                                                                                    >  4:201424/1‑148 (MQ=255)                              agcTTTACACAGCGTACCGCCAGTGTCGAGCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTcagca                                                                                                                  <  2:56886/149‑1 (MQ=255)                                cTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCagcagc                                                                                                                >  8:537460/1‑149 (MQ=255)                                    acacAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTa                                                                                                             <  8:74840/148‑1 (MQ=255)                                     cacaGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAg                                                                                                            <  4:199696/148‑1 (MQ=255)                                     cacaGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGACGATTGCCATATCGGTATCGTTCAGCAGCTTAg                                                                                                            <  2:278328/148‑1 (MQ=255)                                        aGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGa                                                                                                         >  4:211233/1‑148 (MQ=255)                                        aGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTGTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGa                                                                                                         >  5:399889/1‑148 (MQ=255)                                                gCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCggg                                                                                                >  7:491082/1‑149 (MQ=255)                                                gCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCggg                                                                                                <  6:364140/149‑1 (MQ=255)                                                 tactggtCGATGATATGATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGGATCGTTCAGCAGCTTAGCGATAGCGCGGgc                                                                                               >  5:426931/6‑149 (MQ=255)                                                 ccAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCggg                                                                                                >  1:23342/1‑148 (MQ=255)                                                       tCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATa                                                                                                       <  4:129185/135‑1 (MQ=255)                                                       tCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATa                                                                                                       >  3:129185/1‑135 (MQ=255)                                                         gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACaa                                                                                       >  2:50134/1‑149 (MQ=255)                                                         gATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACaa                                                                                       >  5:327883/1‑149 (MQ=255)                                                           tcatatcatCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAAcg                                                                                     >  3:372110/1‑149 (MQ=255)                                                              tatcatCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAAcgccg                                                                                  <  7:537460/149‑1 (MQ=255)                                                                  atcGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGccgccg                                                                               <  6:327883/148‑1 (MQ=255)                                                                  atcGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGccgccg                                                                               <  5:350172/148‑1 (MQ=255)                                                                  atcGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGa                                                                              <  7:210217/149‑1 (MQ=255)                                                                   tcGACCAGTACGCAGTCACGTCCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGAt                                                                             <  2:265294/149‑1 (MQ=255)                                                                   tcGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGAtt                                                                                                                                        <  4:147775/90‑1 (MQ=255)                                                                   tcGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGAtt                                                                                                                                        >  3:147775/1‑90 (MQ=255)                                                                     gACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGt                                                                           >  1:148950/1‑149 (MQ=255)                                                                       ccAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGCTAGCGCGGGCACGCACAACGCCGCCGATGTcc                                                                         <  6:92571/149‑1 (MQ=255)                                                                       ccAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGgcac                                                                                             <  5:423790/129‑1 (MQ=255)                                                                       ccAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGgcac                                                                                             >  6:423790/1‑129 (MQ=255)                                                                        cAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTa                                                                                                             >  3:266585/1‑112 (MQ=255)                                                                        cAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTa                                                                                                             <  4:266585/112‑1 (MQ=255)                                                                        cAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCg                                                                        >  7:268911/1‑149 (MQ=255)                                                                          gTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCGATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGga                                                                      <  5:316295/149‑1 (MQ=255)                                                                           tACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAAcgc                                                                                    <  8:90970/134‑1 (MQ=255)                                                                           tACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAAcgc                                                                                    >  7:90970/1‑134 (MQ=255)                                                                                  tCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACca                                                               >  4:130957/1‑148 (MQ=255)                                                                                        ccTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTgg                                                        <  2:148950/149‑1 (MQ=255)                                                                                        ccTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGACACGCACAACGCCGCCGAt                                                                             >  7:59883/1‑128 (MQ=255)                                                                                        ccTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGACACGCACAACGCCGCCGAt                                                                             <  8:59883/128‑1 (MQ=255)                                                                                         cTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTggg                                                       <  1:366354/149‑1 (MQ=255)                                                                                             aaCGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTAt                                                   <  7:71759/149‑1 (MQ=255)                                                                                              aCGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATc                                                  <  3:61450/149‑1 (MQ=255)                                                                                               cGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATcc                                                 <  3:181890/149‑1 (MQ=255)                                                                                                gTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCa                                                <  5:336214/149‑1 (MQ=255)                                                                                                 tCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATc                                                  >  6:551167/1‑146 (MQ=255)                                                                                                   aCCGATGATATGCATCACCTGTGAAACGTTCGTACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGa                                              <  2:296905/148‑1 (MQ=255)                                                                                                     cGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGAtt                                            >  4:136415/1‑148 (MQ=255)                                                                                                      gatgatATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGAtt                                            >  4:53725/1‑147 (MQ=255)                                                                                                           tatGCATCACCTGTGAAACGTTCGCCCGCGGACGACGTTTTTCGAAGATTGCCATATCGGTATCGTTCCGCAGCTTAGCGATCGCGCGGGCACGCACAACGCCCCCGATGTACGGAGAAACCACAATTTGGTTATCCAGATTCAGcag                                      >  5:134899/1‑146 (MQ=255)                                                                                                           tatGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTg                                      >  8:29078/1‑148 (MQ=255)                                                                                                              gCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGCCACCACAATTGGGTTATCCAGAGTCAGCTgcag                                   >  6:295423/1‑148 (MQ=255)                                                                                                              gCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTgcag                                   >  5:293648/1‑148 (MQ=255)                                                                                                                aTCACCTTTGAAACGTTCGCAGGCGGTCGACGGTTTTCGCTGATGGCCTTATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTgcagca                                 <  6:426931/148‑1 (MQ=255)                                                                                                                aTCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCAt                                >  5:256619/1‑149 (MQ=255)                                                                                                                 tCACCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATg                               >  4:97627/1‑149 (MQ=255)                                                                                                                   aCCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTgc                                     >  7:19735/1‑141 (MQ=255)                                                                                                                   aCCTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTgc                                     <  8:19735/141‑1 (MQ=255)                                                                                                                     cTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCTGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCtt                           >  4:131783/1‑149 (MQ=255)                                                                                                                     cTGTGAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCtt                           >  4:102754/1‑149 (MQ=255)                                                                                                                       gtgAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTc                          >  3:485639/1‑148 (MQ=255)                                                                                                                        tgAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTcc                         <  3:201424/148‑1 (MQ=255)                                                                                                                        tgAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTcc                         <  3:211233/148‑1 (MQ=255)                                                                                                                        tgAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTc                          >  3:27537/1‑147 (MQ=255)                                                                                                                        tgAAACGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTc                          <  3:130957/147‑1 (MQ=255)                                                                                                                          aaaCGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCcag                       >  1:79402/1‑148 (MQ=255)                                                                                                                           aaCGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCcagca                     <  3:102754/149‑1 (MQ=255)                                                                                                                           aaCGTTCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCcagca                     <  4:372110/149‑1 (MQ=255)                                                                                                                               ttCGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGAt                 >  3:51290/1‑149 (MQ=255)                                                                                                                                 cGCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATc                <  4:27537/148‑1 (MQ=255)                                                                                                                                  gCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCgg              >  2:387156/1‑149 (MQ=255)                                                                                                                                  gCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCgg              >  8:252513/1‑149 (MQ=255)                                                                                                                                  gCACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCgg              <  7:487463/149‑1 (MQ=255)                                                                                                                                   cACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCggg             <  1:50134/149‑1 (MQ=255)                                                                                                                                   cACGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCggg             >  6:452213/1‑149 (MQ=255)                                                                                                                                    aCGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGc            >  7:215694/1‑149 (MQ=255)                                                                                                                                    aCGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGc            <  6:256619/149‑1 (MQ=255)                                                                                                                                    aCGCGGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGCATCGGGc            >  1:382886/1‑149 (MQ=255)                                                                                                                                       cgGACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTAc         <  3:97627/149‑1 (MQ=255)                                                                                                                                        ggACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTggg                                                       <  6:420083/102‑1 (MQ=255)                                                                                                                                        ggACGACGTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTggg                                                       >  5:420083/1‑102 (MQ=255)                                                                                                                                            gacgTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCaaa     >  1:491127/1‑148 (MQ=255)                                                                                                                                              cgTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATAc  >  7:433491/1‑149 (MQ=255)                                                                                                                                              cgTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATAc  <  2:382886/149‑1 (MQ=255)                                                                                                                                              cgTTTTTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATAc  >  1:145381/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                TTAGCACCACGTTCTTTCAGAGCTTCAGCAGCTTTACACAGCGTACCGCCAGTGTCGATCATATCATCGACCAGTACGCAGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATAC  >  NC_000913/1261151‑1261440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑