New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 326218837 (1.030)4 (0.120) 4/236 3.5 10.6% coding (231/2295 nt) tdcE pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
?NC_000913 = 3262210 33 (0.990)coding (209/2295 nt) tdcE pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGTTTCAACGGCGCATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATCGCGGACATTAATTTCGTTTTTCCAGTCCGTAC  >  NC_000913/3262084‑3262357
                                                                                                                              |                                                                                                                                                   
cGTTTCAACGGCGCATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCtt                                                                                                                                   >  4:154021/1‑145 (MQ=255)
cGTTTCAACGGCGCATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCtt                                                                                                                                   <  3:154021/145‑1 (MQ=255)
             cATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTcaca                                                                                                                   >  5:8481/1‑148 (MQ=255)
             cATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCca                                                                                                                   >  1:79780/1‑148 (MQ=255)
                   tttGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTcc                                                                                                            <  1:44465/149‑1 (MQ=255)
                                                 ggTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCgtg                                                                                                         <  1:37260/122‑1 (MQ=255)
                                                 ggTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAAATCCgtg                                                                                                         >  2:37260/1‑122 (MQ=255)
                                                  gTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCgtggtg                                                                                                      <  8:59606/124‑1 (MQ=255)
                                                  gTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCgtggtg                                                                                                      >  7:59606/1‑124 (MQ=255)
                                                  gTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGaaa                                                                              <  6:8481/148‑1 (MQ=255)
                                                  gTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGa                                                                                >  1:182596/1‑146 (MQ=255)
                                                          atCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTc                                                                                                                                                 <  3:131806/73‑1 (MQ=255)
                                                          atCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTc                                                                                                                                                 >  4:131806/1‑73 (MQ=255)
                                                             ccGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCt                                                                  >  7:103265/1‑149 (MQ=255)
                                                               gCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGAt                                                                                                                                              <  8:37523/71‑1 (MQ=255)
                                                               gCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGAt                                                                                                                                              >  7:37523/1‑71 (MQ=255)
                                                                  tcatGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCa                                                                                                   >  6:49047/1‑111 (MQ=255)
                                                                  tcatGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCa                                                                                                   <  5:49047/111‑1 (MQ=255)
                                                                    atGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCgt                                                                                                          <  8:23539/102‑1 (MQ=255)
                                                                    atGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCgt                                                                                                          >  7:23539/1‑102 (MQ=255)
                                                                                    gtggCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGtt                                            <  8:103265/148‑1 (MQ=255)
                                                                                     tggCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATa                                                             <  7:62229/130‑1 (MQ=255)
                                                                                     tggCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATa                                                             >  8:62229/1‑130 (MQ=255)
                                                                                              tGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATcg                                 >  1:57735/1‑149 (MQ=255)
                                                                                                gTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATcgcg                               >  5:32186/1‑149 (MQ=255)
                                                                                                 tATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATcgcg                               >  2:197939/1‑148 (MQ=255)
                                                                                                 tATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATcgcg                               <  1:197939/148‑1 (MQ=255)
                                                                                                   tCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGtt                                                 >  1:154288/1‑128 (MQ=255)
                                                                                                   tCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGtt                                                 <  2:154288/128‑1 (MQ=255)
                                                                                                     gAAATGAACCGGCGCGTGTGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGta                                                      <  8:129010/121‑1 (MQ=255)
                                                                                                        aTCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCtt                                                                 <  4:148770/107‑1 (MQ=255)
                                                                                                        aTCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCtt                                                                 >  3:148770/1‑107 (MQ=255)
                                                                                                                     tGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATCGCGGACATTAATTTCGTTTTTCCa          <  2:57735/149‑1 (MQ=255)
                                                                                                                         ttGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATCGCGGACATTAATTTCGTTTTTc            >  4:14394/1‑143 (MQ=255)
                                                                                                                         ttGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATCGCGGACATTAATTTCGTTTTTc            <  3:14394/143‑1 (MQ=255)
                                                                                                                         ttGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATCGCGGACATTAATTTCGTTTTTCCAGTcc      >  2:14803/1‑149 (MQ=255)
                                                                                                                             aTTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATCGCGGACATTAATTTCGTTTTTCCAGTCCGTAc  <  1:14803/149‑1 (MQ=255)
                                                                                                                              |                                                                                                                                                   
CGTTTCAACGGCGCATCCGTTTGCAGGCCAACAATTTTTTCCAGCGGCTGGTTAATATATCCCGCATCATGAGCGGTAATTGTGGTGGCAATATTGGTATCGAAATCAACCGGCGCGTGGGTTGCATTTTCGATACGGATGCCTTCCATTACTTTTTCCCACAATTCCGTGGTGGCAGGCGTCGCTTCGGCGAGGAAAGATTCATCGCCTTCATACGGTGTATAGTTATGTTGAATAAAATCGCGGACATTAATTTCGTTTTTCCAGTCCGTAC  >  NC_000913/3262084‑3262357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.