Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,317,667	T→G	E737A (GAA→GCA)	rcsC ←	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,317,667	0	T	G	100.0%	108.7 / NA	31	E737A (GAA→GCA)	rcsC	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/15);  total (16/15)

TGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAACG  >  NC_000913/2317529‑2317811                                                                                                                                           |                                                                                                                                                 tgtACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtgttg                                                                                                                                         <  3:150719/148‑1 (MQ=255)      ccACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAacga                                                                                                                                   >  6:70179/1‑149 (MQ=255)            cccTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGAt                                                                                                                               <  5:70179/147‑1 (MQ=255)               tGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgc                                                                                                                          >  5:13753/1‑149 (MQ=255)                     tttCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtgt                                                                                                                                           >  3:130241/1‑126 (MQ=255)                     tttCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtgt                                                                                                                                           <  4:130241/126‑1 (MQ=255)                           cAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAAc                                                                                                              >  3:1166/1‑149 (MQ=255)                                         tatGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAAc                                                                                                <  6:13753/149‑1 (MQ=255)                                                               tACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGa                                                                          >  4:45704/1‑149 (MQ=255)                                                                 ccGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGa                                                                          <  3:45704/147‑1 (MQ=255)                                                                                     ttACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTAc                                                     <  4:1166/148‑1 (MQ=255)                                                                                           aCTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgctgcgct                                                                                                                    <  1:9778/79‑1 (MQ=255)                                                                                           aCTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgctgcgct                                                                                                                    >  2:9778/1‑79 (MQ=255)                                                                                                    tCAGTGGTCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCAc                                     >  8:68795/1‑149 (MQ=255)                                                                                                       gTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGcc                                  >  3:76242/1‑149 (MQ=255)                                                                                                             aaCACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCtttttt                            >  1:76599/1‑149 (MQ=255)                                                                                                              acacATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTc                           <  4:76242/149‑1 (MQ=255)                                                                                                                  aTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTg                                                                              >  7:36224/1‑94 (MQ=255)                                                                                                                  aTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTg                                                                              <  8:36224/94‑1 (MQ=255)                                                                                                                   tCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCgg                      >  3:4403/1‑149 (MQ=255)                                                                                                                     ttCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGAc                                                                                    >  7:11660/1‑85 (MQ=255)                                                                                                                     ttCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGAc                                                                                    <  8:11660/85‑1 (MQ=255)                                                                                                                     ttCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCggg                     >  4:24831/1‑148 (MQ=255)                                                                                                                        gggAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTAc                  <  7:68795/148‑1 (MQ=255)                                                                                                                                gTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGc                        >  4:96276/1‑134 (MQ=255)                                                                                                                                gTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGc                        <  3:96276/134‑1 (MQ=255)                                                                                                                                    cTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTa                                                      <  5:2951/100‑1 (MQ=255)                                                                                                                                    cTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTa                                                      >  6:2951/1‑100 (MQ=255)                                                                                                                                    cTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCttt                               <  2:140052/123‑1 (MQ=255)                                                                                                                                    cTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCttt                               >  1:140052/1‑123 (MQ=255)                                                                                                                                       cccTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAACg  <  2:76599/149‑1 (MQ=255)                                                                                                                                           |                                                                                                                                                 TGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAACG  >  NC_000913/2317529‑2317811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑