Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,044,385 Δ1 bp 100% coding (187/1431 nt) yihF → DUF945 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,044,3850T.100.0% 529.5 / NA 116coding (187/1431 nt)yihFDUF945 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (52/64);  total (52/64)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 120 total aligned reads displayed.

CCGTAATCTGGGGTGGTGGCACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCATGGTAATGTC  >  NC_000913/4044239‑4044528
                                                                                                                                                  |                                                                                                                                               
ccGTAATCTGGGGTGGTGGCACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCttt                                                                                                                                               <  7:10644/149‑1 (MQ=255)
 cGTAATCTGGGGTGGTGGCACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCtttt                                                                                                                                              <  1:103351/149‑1 (MQ=255)
 cGTAATCTGGGGTGGTGGCACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCtttt                                                                                                                                              <  7:147570/149‑1 (MQ=255)
   tAATCTGGGGTGGTGGCACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCttttt                                                                                                                                             >  5:375200/1‑148 (MQ=255)
        tGGGGTGGTGGCACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTcc                                                                                                                                      >  4:12029/1‑149 (MQ=255)
             tggtggCACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACACGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGttt                                                                                                                                 <  2:32904/149‑1 (MQ=255)
                   cacaTGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTcaaat                                                                                                                           <  3:3418/149‑1 (MQ=255)
                   cacaTGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTcaaat                                                                                                                           <  6:220268/149‑1 (MQ=255)
                   cacaTGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTcaaat                                                                                                                           <  1:308298/149‑1 (MQ=255)
                    acaTGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTcaaatg                                                                                                                          <  1:216229/149‑1 (MQ=255)
                     caTGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTcaaatg                                                                                                                          >  7:151931/1‑148 (MQ=255)
                       tGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTcaaatgcaa                                                                                                                       <  5:110861/149‑1 (MQ=255)
                        ggTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAATAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCACGTTTTcaaatgcaaa                                                                                                                      >  1:81780/1‑149 (MQ=255)
                        ggTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTcaaatgcaaa                                                                                                                      >  1:303493/1‑149 (MQ=255)
                         ggacacAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTcaaatgcaaa                                                                                                                      <  6:35130/146‑1 (MQ=255)
                          tACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTACCGATGCTAAAAAGAAAGGTGACCATGCATACGATATGACGTTATTTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTcaaatgtaaat                                                                                                                     >  3:95523/1‑148 (MQ=255)
                          tACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTcaaatgcaaat                                                                                                                     >  4:14516/1‑148 (MQ=255)
                          tACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTcaaa                                                                                                                            <  6:357613/141‑1 (MQ=255)
                               aGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACAtt                                                                                                               >  6:80082/1‑149 (MQ=255)
                                    cGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATa                                                                                                          <  7:307440/149‑1 (MQ=255)
                                       aaaTTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACg                                                                                                       >  1:138165/1‑149 (MQ=255)
                                        aaTTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACgg                                                                                                      >  5:172319/1‑149 (MQ=255)
                                             aGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCa                                                                                                  <  3:12286/148‑1 (MQ=255)
                                             aGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCa                                                                                                  <  8:111876/148‑1 (MQ=255)
                                             aGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCAc                                                                                                 >  2:339586/1‑149 (MQ=255)
                                             aGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCAc                                                                                                 >  7:377163/1‑149 (MQ=255)
                                              gCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCAcc                                                                                                <  8:377163/149‑1 (MQ=255)
                                              gCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCAcc                                                                                                <  3:204952/149‑1 (MQ=255)
                                               ccTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCAccc                                                                                               >  6:74566/1‑149 (MQ=255)
                                                   gtgtCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGAtc                                                                                           >  3:357737/1‑149 (MQ=255)
                                                      tCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGAtctc                                                                                         >  8:194846/1‑148 (MQ=255)
                                                        gAATAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACTTGCCTACGATTTGACGTTATGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCatt                                                                                      <  6:327905/149‑3 (MQ=255)
                                                        gAAAAATTTATTAAAGATTTTAACGATTCTAAAAAGAAAGGTGAACATGCCTACGATATGACTTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCaa                                                                                       <  4:78686/148‑1 (MQ=255)
                                                        gAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCaa                                                                                       >  3:264482/1‑148 (MQ=255)
                                                        gAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAat                                                                                      <  5:268574/149‑1 (MQ=255)
                                                         aaaaaTTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAata                                                                                     >  2:212358/1‑149 (MQ=255)
                                                         aaaaaTTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAata                                                                                     <  2:329019/149‑1 (MQ=255)
                                                             aTTTATTAAAGATTTTAACGATGCTAAAAAGACAGGTGAACCTGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCGTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCaa                                                                                 <  2:150792/149‑1 (MQ=255)
                                                                tattaAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAgc                                                                               <  3:12029/148‑1 (MQ=255)
                                                                  ttaAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAgtcag                                                                            >  8:24936/1‑149 (MQ=255)
                                                                  ttaAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAgccag                                                                            <  1:291983/149‑1 (MQ=255)
                                                                     aaGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAgccaggc                                                                          >  7:343268/1‑148 (MQ=255)
                                                                     aaGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAgccaggc                                                                          >  7:86206/1‑148 (MQ=255)
                                                                     aaGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAgccaggc                                                                          >  1:382141/1‑148 (MQ=255)
                                                                     aaGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTCTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAgccaggc                                                                          >  5:34850/1‑148 (MQ=255)
                                                                      aGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAgccaggcca                                                                        <  1:356689/149‑1 (MQ=255)
                                                                       gATTTTAACGATGCTAAAAAGAAAGGGTAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATACCCGTTTTCAAATGTAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAgccaggccag                                                                       <  4:95523/149‑1 (MQ=255)
                                                                         ttttAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGTCAGGCCAGa                                                                      >  2:177838/1‑148 (MQ=255)
                                                                              aCGATGCTAAAAAGTAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGtt                                                                 <  2:131322/148‑1 (MQ=255)
                                                                              aCGATGCTAAAAAGATAGGGTAACATGCCTACGATATGACGTTAAGTTATCTAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGTTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGtt                                                                 <  8:371789/148‑1 (MQ=255)
                                                                              aCGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGtt                                                                 <  4:221312/148‑1 (MQ=255)
                                                                              aCGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGtt                                                                 <  8:187728/148‑1 (MQ=255)
                                                                              aCGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGtt                                                                 <  7:284017/148‑1 (MQ=255)
                                                                                 aTGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGTCAGGCCAGAAAGTTGTAt                                                             <  1:177838/149‑1 (MQ=255)
                                                                                  tGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAAATCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTAtt                                                            <  6:275346/149‑1 (MQ=255)
                                                                                     tAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGAAAAAGGCTTTTTAATTCCCGTTTTCAAATTCAAATGACTTTCGATAACGGTGCAACCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGa                                                         >  5:32588/1‑149 (MQ=255)
                                                                                      aaaaaGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGAt                                                        >  1:59652/1‑149 (MQ=255)
                                                                                       aaaaGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTAAATTACCGATTTCAAATGCAAATGACATTCGATAACTGTTCACACGATATCAATATCAAGACAGGACAGAAAGTTGTATTTGAtg                                                       >  1:198775/1‑149 (MQ=255)
                                                                                        aaagaaagGTGAACATGACTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGAtgt                                                      <  6:239732/149‑1 (MQ=255)
                                                                                         aagaaagGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGAtgtg                                                     >  4:281116/1‑149 (MQ=255)
                                                                                            aaagGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGAt                                                  <  2:157703/149‑1 (MQ=255)
                                                                                            aaagGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGAt                                                  <  5:49281/149‑1 (MQ=255)
                                                                                             aagGTGAACATGCATACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATg                                                 <  5:74566/149‑1 (MQ=255)
                                                                                              agGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCAAGAAAGTTGTATTTGATGTGGATGt                                                >  6:338623/1‑149 (MQ=255)
                                                                                                 tGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGa                                             <  5:4851/149‑1 (MQ=255)
                                                                                                  gAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAg                                            >  3:358910/1‑149 (MQ=255)
                                                                                                    aCATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCa                                          <  7:92336/149‑1 (MQ=255)
                                                                                                        gCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGt                                      >  1:151529/1‑149 (MQ=255)
                                                                                                             cGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGtt                                 <  7:124/149‑1 (MQ=255)
                                                                                                             cGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGt                                  <  5:80082/148‑1 (MQ=255)
                                                                                                                tattaagTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCAGTTTTCAAATGCAAATGAAATTCTATAACGGTGCACCCTATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGc                               <  2:81780/142‑1 (MQ=255)
                                                                                                                tatGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAat                                                                                      <  1:395832/93‑1 (MQ=255)
                                                                                                                tatGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAat                                                                                      >  2:395832/1‑93 (MQ=255)
                                                                                                                      gTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATca                         >  2:67655/1‑148 (MQ=255)
                                                                                                                           gTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGt                   <  4:6651/149‑1 (MQ=255)
                                                                                                                           gTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGt                   <  5:276061/149‑1 (MQ=255)
                                                                                                                            ttATCAATATTTTGACAATGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACTGTGCTCCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGtt                  <  6:375200/149‑1 (MQ=255)
                                                                                                                             tATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATACCGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGtt                  >  2:359146/1‑148 (MQ=255)
                                                                                                                              aTCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGCCATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGAACGGTCCGTTGCCCATCACAATGTaaa                >  5:178663/1‑149 (MQ=255)
                                                                                                                               tCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAAt               <  2:138165/149‑1 (MQ=255)
                                                                                                                               tCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAAt               <  6:19198/149‑1 (MQ=255)
                                                                                                                                 aaaaTTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGaa                                                                     <  8:395861/93‑1 (MQ=255)
                                                                                                                                 aaaaTTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGaa                                                                     >  7:395861/1‑93 (MQ=255)
                                                                                                                                 aaaaTTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAAt               >  6:296779/1‑147 (MQ=255)
                                                                                                                                 aaaaTTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAAt               <  5:296779/147‑1 (MQ=255)
                                                                                                                                  aaaTTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCa            <  6:172319/149‑1 (MQ=255)
                                                                                                                                  aaaTTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCa            <  2:151529/149‑1 (MQ=255)
                                                                                                                                  aaaTTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCa            <  4:357737/149‑1 (MQ=255)
                                                                                                                                  aaaTTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACCGTGCACCCGATCTCAATATCAAGCCAGGCCTGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACATTGTTAATGCa            >  3:20571/1‑149 (MQ=255)
                                                                                                                                   aaTTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCAt           <  2:59652/149‑1 (MQ=255)
                                                                                                                                     ttttGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCATgg         >  6:346196/1‑149 (MQ=255)
                                                                                                                                     ttttGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCATgg         >  6:311892/1‑149 (MQ=255)
                                                                                                                                     ttttGACAAAGGCTTTTTAATTCCCCTTTTCCAATGCAAATGACATTCGTTAACGGTGCACCCGATCTCAAAAACCAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCATgg         >  4:10779/1‑149 (MQ=255)
                                                                                                                                      tttGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCATgg         >  4:268608/1‑148 (MQ=255)
                                                                                                                                         gACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCATGGTaa      <  7:194846/148‑1 (MQ=255)
                                                                                                                                         gACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGAGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCATGGTaa      <  8:151931/148‑1 (MQ=255)
                                                                                                                                          aCAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCATGGTAAt     <  3:268608/148‑1 (MQ=255)
                                                                                                                                           ccaaGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAAAGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCATTGTAATGt   <  5:122453/147‑1 (MQ=255)
                                                                                                                                           cAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCATGGTAATGt   >  3:213294/1‑149 (MQ=255)
                                                                                                                                            aaaGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCATGGTAATGTc  <  2:382141/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CCGTAATCTGGGGTGGTGGCACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATGCATGGTAATGTC  >  NC_000913/4044239‑4044528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: