Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,139,640 C→A 6.4% intergenic (+47/+76) yghU → / ← hybG putative S‑transferase/hydrogenase 2 accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,139,6400CA 6.4% 239.9 / 2.4 70intergenic (+47/+76)yghU/hybGputative S‑transferase/hydrogenase 2 accessory protein
Reads supporting (aligned to +/- strand):  ref base C (35/30);  new base A (3/2);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGAT  >  NC_000913/3139496‑3139787
                                                                                                                                                |                                                                                                                                                   
ttGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgc                                                                                                                                                 <  6:27310/149‑1 (MQ=255)
  gTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGAATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgccg                                                                                                                                               >  3:220650/1‑149 (MQ=255)
         gCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                                        >  3:115440/1‑149 (MQ=255)
           aCCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                      <  6:221942/149‑2 (MQ=255)
              aaCGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCATGGTTAAGGGTTGGTGTTCGTCGCAGCAAGCAATCCAGGCCGGATAAGGCGTTAACGCCGAATCCGGCAATc                                                                                                                                   >  6:368493/1‑149 (MQ=255)
                 ggACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATGCGGCGTGAACGCCTTATCCGGcctggatg                                                                                                                                <  4:115440/149‑8 (MQ=38)
                      gCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACaa                                                                                                                           <  3:146510/149‑1 (MQ=255)
                          aaTGAGCAGTTGAATGAGCGCCATGACGCCAGTGATTTCGAGAAGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGc                                                                                                                        <  4:220650/148‑1 (MQ=255)
                             gAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGa                                                                                                                    >  4:336532/1‑149 (MQ=255)
                                      cATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCt                                                                                                           <  5:368493/149‑1 (MQ=255)
                                           gcgcCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCcgc                                                                                                      <  3:294910/149‑1 (MQ=255)
                                             gcCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGt                                                                                                    >  6:253670/1‑149 (MQ=255)
                                               cATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCt                                                                                                  >  5:68910/1‑149 (MQ=255)
                                                 tGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAGGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTa                                                                                                >  8:129549/1‑149 (MQ=255)
                                                   aCGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATc                                                                                              <  8:166441/149‑1 (MQ=255)
                                                   aCGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGGCAGGGGTAAGGGGTGGTGGTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATc                                                                                              <  4:126081/149‑1 (MQ=255)
                                                       cAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGc                                                                                          >  4:100413/1‑149 (MQ=255)
                                                           gATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTAc                                                                                      >  7:100928/1‑149 (MQ=255)
                                                             tttCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACg                                                                                     >  1:78138/1‑148 (MQ=255)
                                                                cGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGaaa                                                                                  <  7:355606/148‑1 (MQ=255)
                                                                     cGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAat                                                                            <  3:353027/149‑1 (MQ=255)
                                                                      gAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatc                                                                           <  1:241602/149‑1 (MQ=255)
                                                                      gAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAat                                                                            >  6:294222/1‑148 (MQ=255)
                                                                         tACGGAAGATTAGCGTCAGGGGGAAGGGTGTGTGATCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatcatg                                                                        <  5:265576/149‑2 (MQ=37)
                                                                         tACGGAAGATCAGCGGGAGGGGTAAGGGTTGGTGGTCGACGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatcatg                                                                        <  5:265577/149‑2 (MQ=37)
                                                                         tACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatcatc                                                                        <  8:100928/149‑1 (MQ=255)
                                                                            ggAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcg                                                                     >  6:110379/1‑149 (MQ=255)
                                                                             gAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcgc                                                                    <  3:254400/149‑1 (MQ=255)
                                                                             gAAGATAAGCGTCACGGGTAAGGGTTGGTGTTAGTCGCAGCAAGCCATCCAGGCCGGATAATGCGTTCACGCCGCAACCGGCAATCGTGCACCATGCCTGATGCGACGCAGCCGCGTCTAATCAGGCCTACGAACGCTACTCCTCCcgc                                                                    >  4:20624/1‑149 (MQ=255)
                                                                              aaGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGaaa                                                                                  <  3:313112/134‑1 (MQ=255)
                                                                              aaGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGaaa                                                                                  >  4:313112/1‑134 (MQ=255)
                                                                                 aTAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTg                                                                <  5:95473/149‑1 (MQ=255)
                                                                                     gCGTCAGGGGTAAGGGTTCGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCCTTAACCCCGCATCCGGCAAACGTGCACAATGCCAGAAGCGACGCAGCCGCGTCTTATAAGGACTACGAAAGCTAATCAACACGCGCTGGTaa                                                            >  8:176614/1‑149 (MQ=255)
                                                                                            gggTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGt                                                      >  8:197534/1‑148 (MQ=255)
                                                                                            gggTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGt                                                      >  1:124332/1‑148 (MQ=255)
                                                                                            gggTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTa                                                     <  5:253670/149‑1 (MQ=255)
                                                                                             ggTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTaa                                                    >  5:377740/1‑149 (MQ=255)
                                                                                              gTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAAt                                                   >  1:137361/1‑149 (MQ=255)
                                                                                                    gTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCAttt                                             <  2:223762/149‑1 (MQ=255)
                                                                                                     ttGGTGTTCGTCGCAGCAGGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTg                                            <  7:129549/149‑1 (MQ=255)
                                                                                                        gtgtTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGgcg                                         >  8:111280/1‑149 (MQ=255)
                                                                                                        gtgtTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGgc                                          <  5:110379/148‑1 (MQ=255)
                                                                                                           ttCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAg                                      >  8:220647/1‑149 (MQ=255)
                                                                                                               tcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAAAGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCg                                  >  6:62055/1‑149 (MQ=255)
                                                                                                                cgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCGGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTGTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGt                                 <  5:382834/149‑1 (MQ=255)
                                                                                                                 gcagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTc                                >  6:274414/1‑149 (MQ=255)
                                                                                                                   agcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCt                               >  3:347844/1‑148 (MQ=255)
                                                                                                                     caAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAAt                            <  5:62055/149‑1 (MQ=255)
                                                                                                                        gCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAAtgtg                         <  2:124332/149‑1 (MQ=255)
                                                                                                                         ccATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtgg                        >  4:363783/1‑149 (MQ=255)
                                                                                                                          cctCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggc                       >  7:263528/3‑149 (MQ=255)
                                                                                                                           aTCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggct                      >  1:115596/1‑149 (MQ=255)
                                                                                                                            tCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctt                     >  1:367727/1‑149 (MQ=255)
                                                                                                                            tCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGAGTCTTATCAGGCCTACGAAAGCTAATCATCCCGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctt                     >  5:133526/1‑149 (MQ=255)
                                                                                                                              cAGGCCGGATAATGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggcttt                    <  5:294222/148‑1 (MQ=255)
                                                                                                                                   cGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttggctt               >  3:357527/1‑148 (MQ=255)
                                                                                                                                   cGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttggctt               >  7:75730/1‑148 (MQ=255)
                                                                                                                                    ggATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttg                   <  6:361197/143‑1 (MQ=255)
                                                                                                                                    ggATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttg                   >  5:361197/1‑143 (MQ=255)
                                                                                                                                     gATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCAt            <  2:137361/149‑1 (MQ=255)
                                                                                                                                      aTAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATc           <  7:176614/149‑1 (MQ=255)
                                                                                                                                       tAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggct                      >  2:368464/1‑137 (MQ=255)
                                                                                                                                       tAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggct                      <  1:368464/137‑1 (MQ=255)
                                                                                                                                         aGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTtc        >  3:256335/1‑149 (MQ=255)
                                                                                                                                         aGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTtc        >  3:120742/1‑149 (MQ=255)
                                                                                                                                          ggCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATcc                                                 >  3:311925/1‑107 (MQ=255)
                                                                                                                                          ggCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATcc                                                 <  4:311925/107‑1 (MQ=255)
                                                                                                                                          ggCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttggctt               >  2:175078/1‑141 (MQ=255)
                                                                                                                                          ggCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttggctt               <  1:175078/141‑1 (MQ=255)
                                                                                                                                          ggCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTtcg       >  4:244449/1‑149 (MQ=255)
                                                                                                                                                cACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCgat  <  5:274414/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGAT  >  NC_000913/3139496‑3139787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: