Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,454,593 G→A 5.0% intergenic (+15/+18) yjfF → / ← fbp putative sugar ABC transporter permease/fructose‑1,6‑bisphosphatase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,454,5930GA 5.0% 322.5 / 4.0 99intergenic (+15/+18)yjfF/fbpputative sugar ABC transporter permease/fructose‑1,6‑bisphosphatase I
Reads supporting (aligned to +/- strand):  ref base G (48/46);  new base A (3/2);  total (51/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

AGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATC  >  NC_000913/4454447‑4454736
                                                                                                                                                  |                                                                                                                                               
aGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGtgtg                                                                                                                                               <  3:193062/149‑1 (MQ=255)
  ttCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGtgtg                                                                                                                                               >  3:37875/1‑147 (MQ=255)
  ttCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGtgtg                                                                                                                                               >  7:56112/1‑147 (MQ=255)
     cTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGagag                                                                                                                                          <  2:53945/149‑1 (MQ=255)
     cTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGagag                                                                                                                                          <  3:134058/149‑1 (MQ=255)
     cTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGagag                                                                                                                                          <  7:367466/149‑1 (MQ=255)
        gtggACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGgcg                                                                                                                                       >  6:215386/1‑149 (MQ=255)
           gACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGtgtg                                                                                                                                               <  6:35458/138‑1 (MQ=255)
           gACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGtgtg                                                                                                                                               >  5:35458/1‑138 (MQ=255)
           gACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCa                                                                                                                                     <  3:313043/148‑1 (MQ=255)
            aCGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAg                                                                                                                                    >  6:363813/1‑148 (MQ=255)
             cGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTCCAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGga                                                                                                                                  <  5:265012/149‑1 (MQ=255)
                aaaTCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGCGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCTCAGGAGAt                                                                                                                               >  7:121015/1‑149 (MQ=255)
                 aaTCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGAt                                                                                                                               >  6:83984/1‑148 (MQ=255)
                  aTCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTa                                                                                                                             >  5:39377/1‑149 (MQ=255)
                  aTCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTa                                                                                                                             <  8:14569/149‑1 (MQ=255)
                     gCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTAcgc                                                                                                                          <  6:232771/149‑1 (MQ=255)
                        aTCGGTATTTTGTTGTTTATTTTTTTAGCATTACACCGAGGATTAACGGTGCAGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGAGTGGAGAGGCGCAGGAGATTACGCGTc                                                                                                                       >  8:182052/1‑149 (MQ=255)
                        aTCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTc                                                                                                                       >  5:39476/1‑149 (MQ=255)
                           ggTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCg                                                                                                                     <  4:175892/148‑1 (MQ=255)
                            gTATTTTGTTGTTTATTATTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGGCAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCgg                                                                                                                    >  2:249562/1‑148 (MQ=255)
                             tATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCggg                                                                                                                   >  1:266716/1‑148 (MQ=255)
                                    ttgttTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCAc                                                                                                            <  5:20771/148‑1 (MQ=255)
                                      gttTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACgg                                                                                                          <  3:355776/148‑1 (MQ=255)
                                      gttTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGa                                                                                                         >  5:154892/1‑149 (MQ=255)
                                      gttTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGa                                                                                                         >  8:43767/1‑149 (MQ=255)
                                      gttTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGAAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGa                                                                                                         >  6:136937/1‑149 (MQ=255)
                                        ttATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACg                                                                                                           <  1:112404/145‑1 (MQ=255)
                                        ttATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACg                                                                                                           >  2:112404/1‑145 (MQ=255)
                                          aTTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATa                                                                                                       >  2:391206/1‑147 (MQ=255)
                                             tttATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGc                                                                                                   <  6:283170/148‑1 (MQ=255)
                                             tttATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGc                                                                                                   <  6:151496/148‑1 (MQ=255)
                                                   gCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGAc                                                                                            <  7:254026/149‑1 (MQ=255)
                                                    cATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACa                                                                                           <  5:215386/149‑1 (MQ=255)
                                                     aTTACAGCGTGGATTAACGGTGCTATGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACAt                                                                                          >  5:320537/1‑149 (MQ=255)
                                                       tACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCTGGAACTCACGGATAAAGCGTTCGACATc                                                                                         <  6:370757/148‑1 (MQ=255)
                                                        aCAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCtt                                                                                       >  4:238801/1‑149 (MQ=255)
                                                        aCAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCt                                                                                        <  6:39377/148‑1 (MQ=255)
                                                        aCAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCt                                                                                        <  6:39476/148‑1 (MQ=255)
                                                            cGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAAc                                                                                   >  7:75758/1‑149 (MQ=255)
                                                                gATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGGCAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCata                                                                               <  1:249562/149‑1 (MQ=255)
                                                                gATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCata                                                                               >  7:234337/1‑149 (MQ=255)
                                                                  ttAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATTAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATg                                                                             <  1:125819/149‑1 (MQ=255)
                                                                   tAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATgg                                                                            >  5:215696/1‑149 (MQ=255)
                                                                    aaCGGTGCTATGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATgg                                                                            <  6:320537/148‑1 (MQ=255)
                                                                        gTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGtt                                                                       <  5:193376/149‑1 (MQ=255)
                                                                        gTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGtt                                                                       <  6:154892/149‑1 (MQ=255)
                                                                           cTGTGGGAGAATCGTCAGAGGTCGCCAGGGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGcc                                                                    <  3:253606/149‑1 (MQ=255)
                                                                              tgGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGAc                                                                 <  8:121015/149‑1 (MQ=255)
                                                                                 gagaATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACaaa                                                              <  3:325958/149‑1 (MQ=255)
                                                                                 gagaATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACaaa                                                              <  7:43767/149‑1 (MQ=255)
                                                                                   gaATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAg                                                             >  5:302216/1‑148 (MQ=255)
                                                                                     aTCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAAt                                                          <  6:215696/149‑1 (MQ=255)
                                                                                     aTCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAAt                                                          <  3:238801/149‑1 (MQ=255)
                                                                                      tCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAAt                                                          >  1:35334/1‑148 (MQ=255)
                                                                                        gTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGACCTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGaa                                                       <  2:320680/149‑1 (MQ=255)
                                                                                        gTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGaa                                                       >  7:335042/1‑149 (MQ=255)
                                                                                         tCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATg                                                                             >  4:299577/1‑126 (MQ=255)
                                                                                         tCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATg                                                                             <  3:299577/126‑1 (MQ=255)
                                                                                         tCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAAc                                                      <  6:302216/149‑1 (MQ=255)
                                                                                            gagTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCACGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCAAATGGTCGTTGCCGACAAAGAATGAACGgc                                                   >  1:345136/1‑149 (MQ=255)
                                                                                                  gCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGt                                             >  8:117687/1‑149 (MQ=255)
                                                                                                  gCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGt                                             <  1:241646/149‑1 (MQ=255)
                                                                                                     aGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCa                                          <  6:144159/149‑1 (MQ=255)
                                                                                                        gACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGACCTCACGGATAAAGCGTTCGACATCTTCACCCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAggg                                       >  1:140019/1‑149 (MQ=255)
                                                                                                         aCAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGt                                      >  6:363545/1‑149 (MQ=255)
                                                                                                         aCAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGt                                      >  7:315888/1‑149 (MQ=255)
                                                                                                           aaGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCa                                                                                     <  7:78894/100‑1 (MQ=255)
                                                                                                              agTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCg                                 <  5:363813/149‑1 (MQ=255)
                                                                                                               gTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCgg                                >  4:150360/1‑149 (MQ=255)
                                                                                                               gTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCgg                                <  7:165950/149‑1 (MQ=255)
                                                                                                               gTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGGCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAAGCATATGGTCGTTGCGGAGAAAGCTTGAACGGCGCTGGGGCAGGGTGTCCgg                                >  1:346667/1‑149 (MQ=255)
                                                                                                                tCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCgg                                >  5:295419/1‑148 (MQ=255)
                                                                                                                tCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCgg                                >  8:257497/1‑148 (MQ=255)
                                                                                                                   aCATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATCTGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGgatg                            <  2:266716/149‑1 (MQ=255)
                                                                                                                   aCATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGgat                             >  8:311565/1‑148 (MQ=255)
                                                                                                                     aTTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGgatgat                          <  6:295419/149‑1 (MQ=255)
                                                                                                                     aTTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCAGATGGTGGTTGCCGTCAAAGAATGAACGGCGGTGGTGCAGGGTTTCCGGgatga                           >  6:302839/1‑148 (MQ=255)
                                                                                                                        gcgcAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGatat                        <  5:83984/148‑1 (MQ=255)
                                                                                                                          gcagcaATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCa                     <  8:234337/149‑1 (MQ=255)
                                                                                                                           cagcaATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATc                       >  1:377289/1‑146 (MQ=255)
                                                                                                                            agcaATAAAAACGCCTCTCCGTGTGGAGAGGCGCTGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGAGATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGGTGGTGCAGGGTTTCCGGGATGATATc                       >  1:346871/1‑145 (MQ=255)
                                                                                                                              caATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGaa                  >  4:314965/1‑148 (MQ=255)
                                                                                                                              caATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAAt                 <  8:335042/149‑1 (MQ=255)
                                                                                                                                  tcctgcGCCTCTCCACACGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGc             >  8:248513/6‑149 (MQ=255)
                                                                                                                                  aaaaaCGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGc             >  1:231048/1‑149 (MQ=255)
                                                                                                                                   aaaaCGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTAACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGct            >  1:350628/1‑149 (MQ=255)
                                                                                                                                    ctgcGCCTCTCCACACGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGctc           >  1:273282/4‑149 (MQ=255)
                                                                                                                                    aaaCGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGa                                                            <  5:368449/100‑1 (MQ=255)
                                                                                                                                    aaaCGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGa                                                            >  6:368449/1‑100 (MQ=255)
                                                                                                                                    aaaCGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGctc           <  8:164214/149‑1 (MQ=255)
                                                                                                                                     tgcGCCTCTCCACACGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGga                              <  4:299185/127‑1 (MQ=255)
                                                                                                                                      aCGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCtt         >  5:335309/1‑149 (MQ=255)
                                                                                                                                       cGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCttt        <  5:136937/149‑1 (MQ=255)
                                                                                                                                          ctctCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGACCTCACGGATAAAGCGGTCGCCATCTTCACCCATATGGGCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGGTCTTTGcc     >  6:273284/1‑149 (MQ=255)
                                                                                                                                          ctctCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGATCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGc      >  4:113093/1‑148 (MQ=255)
                                                                                                                                             tcCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCAtc  >  4:221354/1‑149 (MQ=255)
                                                                                                                                             tcCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCAtc  >  1:340634/1‑149 (MQ=255)
                                                                                                                                               cGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGaa                                                       <  8:209645/94‑1 (MQ=255)
                                                                                                                                               cGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGaa                                                       >  7:209645/1‑94 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATC  >  NC_000913/4454447‑4454736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: