Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,439,588 G→C 6.7% intergenic (‑79/+28) yhdN ← / ← rplQ DUF1992 family protein/50S ribosomal subunit protein L17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,439,5880GC 6.7% 376.3 / 12.3 113intergenic (‑79/+28)yhdN/rplQDUF1992 family protein/50S ribosomal subunit protein L17
Reads supporting (aligned to +/- strand):  ref base G (63/42);  new base C (4/4);  total (67/46)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

GGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGG  >  NC_000913/3439440‑3439733
                                                                                                                                                    |                                                                                                                                                 
ggTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACccgccg                                                                                                                                                   <  6:185389/149‑1 (MQ=255)
 gTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCgg                                                                                                                                                  >  5:151362/1‑149 (MQ=255)
 gTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCgg                                                                                                                                                  <  3:21064/149‑1 (MQ=255)
       aaaCTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCggg                                                                                                                                            >  8:247435/1‑149 (MQ=255)
        aaCTCACCTTTCGCTTGCTCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGt                                                                                                                                           <  7:368314/149‑1 (MQ=255)
        aaCTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGt                                                                                                                                           >  1:116879/1‑149 (MQ=255)
         aCTCACCTTTAGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCAAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGtt                                                                                                                                          >  3:270984/1‑149 (MQ=255)
             aCCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGtttttt                                                                                                                                      <  3:155195/149‑1 (MQ=255)
             aCCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCCCGGCGGGtttt                                                                                                                                        <  6:79628/147‑1 (MQ=255)
              ccTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGttttttt                                                                                                                                     <  1:227393/149‑1 (MQ=255)
              ccTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGtttttt                                                                                                                                      >  1:45481/1‑148 (MQ=255)
              ccTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGtttttt                                                                                                                                      >  1:89178/1‑148 (MQ=255)
                     cTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGc                                                                                                                              >  1:221126/1‑149 (MQ=255)
                          gcTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCtt                                                                                                                            >  8:170272/1‑146 (MQ=255)
                            ttCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAATAGAGTACCGCTATTGTAGATAAGTGGTGATACTAGGGGTATAAAAAAACCCGCAGGGGCGGGTTTTTTTACGTTGCTTCAGag                                                                                                                       >  5:91122/1‑148 (MQ=255)
                            ttCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCCCGGCGGGttttt                                                                                                                                       >  4:185202/1‑133 (MQ=255)
                            ttCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCCCGGCGGGttttt                                                                                                                                       <  3:185202/133‑1 (MQ=255)
                              cTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTa                                                                                                                     >  7:170641/1‑149 (MQ=255)
                                 cTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTActc                                                                                                                  <  3:258974/149‑1 (MQ=255)
                                   atatGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTg                                                                                                                >  8:361546/1‑149 (MQ=255)
                                                cccACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTc                                                                                                      <  5:191605/146‑1 (MQ=255)
                                                cccACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTc                                                                                                      <  8:237562/146‑1 (MQ=255)
                                                cccACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGc                                                                                                   >  5:116003/1‑149 (MQ=255)
                                                   aCTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGtttttt                                                                                                                                      >  4:325859/1‑111 (MQ=255)
                                                   aCTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGtttttt                                                                                                                                      <  3:325859/111‑1 (MQ=255)
                                                   aCTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGAAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCttt                                                                                                >  7:119757/1‑149 (MQ=255)
                                                    cTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACg                                                                                                                                  >  2:188635/1‑114 (MQ=255)
                                                    cTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACg                                                                                                                                  <  1:188635/114‑1 (MQ=255)
                                                     tGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTg                                                                                                                               <  6:14050/116‑1 (MQ=255)
                                                       gTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtct                                                                                              <  4:315562/147‑1 (MQ=255)
                                                       gTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTctct                                                                                            >  3:284893/1‑149 (MQ=255)
                                                          aaGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGaa                                                                                         >  5:331046/1‑149 (MQ=255)
                                                           aGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAAc                                                                                        <  7:170272/149‑1 (MQ=255)
                                                            gTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAAc                                                                                        >  8:325968/1‑148 (MQ=255)
                                                               aCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATc                                                                                    <  1:173906/149‑1 (MQ=255)
                                                                ccACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATc                                                                                    >  8:316059/1‑148 (MQ=255)
                                                                ccACATAAATAATCCAGGGGATGAACAAAAAGAGTACATCTATTGTAGATCAGTGGGGATACTACGGGTAAAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCa                                                                                   <  6:359265/149‑1 (MQ=255)
                                                                 cacaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCaa                                                                                  <  6:221980/149‑1 (MQ=255)
                                                                  acaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAc                                                                                 >  3:24225/1‑149 (MQ=255)
                                                                   caTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAc                                                                                 >  1:116518/1‑148 (MQ=255)
                                                                    aTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCa                                                                               <  6:54970/149‑1 (MQ=255)
                                                                    aTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCa                                                                               <  2:45481/149‑1 (MQ=255)
                                                                        aTACACCAGGGGATGAACAAACAGAGTACAGCTATTGTAGATAAGGGGGGATACTACGGGTATAAACAAACCCGCCGGGGCGGGTTTTTTTACGGTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTc                                                                           <  2:258806/149‑1 (MQ=255)
                                                                         tACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTc                                                                                                      >  6:14486/1‑121 (MQ=255)
                                                                         tACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTc                                                                                                      <  5:14486/121‑1 (MQ=255)
                                                                           cTCCAGGGGATGAACAAAAAGAGTACAGCTATTGAAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGa                                                                         <  8:119757/148‑1 (MQ=255)
                                                                            tCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATg                                                                       >  7:176288/1‑149 (MQ=255)
                                                                            tCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAACTGGGGATACTACGGGTATAAAAAAACCCGCAGGGTCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATg                                                                       >  1:261517/1‑149 (MQ=255)
                                                                              cAGGGGATGAACAAAAAGAGTACAGCTATTGTAGAGAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGCTTACTATGCAGCAGCTTCTGCTGTCTCTGAACGATCAACCAGCTCGATGt                                                                      >  8:332383/1‑148 (MQ=255)
                                                                                      gaagaaAAAGAGTACATCTATTGTTGATATGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCATCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTaa                                                                    <  2:281065/142‑1 (MQ=255)
                                                                                        aCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgc                                                           <  8:170641/149‑1 (MQ=255)
                                                                                        aCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCAGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgc                                                           >  1:166407/1‑149 (MQ=255)
                                                                                         cAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGATTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcg                                                          >  6:31482/1‑149 (MQ=255)
                                                                                           aaaaGAGTACAGCTATAGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGATTCAGATTACTCTGACTCAGATTCTCATTTCTCTGAACGAACAAACAGCTCGATGTAACCCATCcgcgcg                                                        >  8:106172/1‑149 (MQ=255)
                                                                                            aaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGAt                                                                        <  7:351199/132‑1 (MQ=255)
                                                                                            aaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGAt                                                                        >  8:351199/1‑132 (MQ=255)
                                                                                            aaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGt                                                       >  5:57548/1‑149 (MQ=255)
                                                                                                agTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTc                                                   <  7:316059/149‑1 (MQ=255)
                                                                                                     aGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCt                                               >  3:98528/1‑148 (MQ=255)
                                                                                                     aGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTg                                              >  1:265384/1‑149 (MQ=255)
                                                                                                     aGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCCCGGCGGGttttt                                                                                                                                       >  8:263625/1‑60 (MQ=37)
                                                                                                          ttGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACg                                          >  5:210950/1‑148 (MQ=255)
                                                                                                          ttGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACg                                          >  4:113820/1‑148 (MQ=255)
                                                                                                             tAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGaa                                       <  7:361546/148‑1 (MQ=255)
                                                                                                             tAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGaa                                       <  7:325968/148‑1 (MQ=255)
                                                                                                             tAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAg                                      >  5:16654/1‑149 (MQ=255)
                                                                                                              aGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCATCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACTCTCAACACGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGc                                     >  2:278152/1‑149 (MQ=255)
                                                                                                               gATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAAc                                                                                        <  3:268159/97‑1 (MQ=255)
                                                                                                               gATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAAc                                                                                        >  4:268159/1‑97 (MQ=255)
                                                                                                                   aGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCcaca                                 <  3:185854/148‑1 (MQ=255)
                                                                                                                       gggATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACtt                              >  8:25572/1‑147 (MQ=255)
                                                                                                                         gATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCACCCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAg                           >  7:98273/1‑148 (MQ=255)
                                                                                                                         gATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgc                                                           >  7:64917/1‑116 (MQ=255)
                                                                                                                         gATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAg                           >  5:99994/1‑148 (MQ=255)
                                                                                                                         gATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCAAGGAAGCCACACTTCAg                           >  5:142178/1‑148 (MQ=255)
                                                                                                                           tactacGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTTAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAAt                        <  4:98528/149‑1 (MQ=255)
                                                                                                                            actacGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTaa                                                                    <  6:183124/104‑1 (MQ=255)
                                                                                                                            actacGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTaa                                                                    >  5:183124/1‑104 (MQ=255)
                                                                                                                              tacGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCAACCTTCAGAATAc                      >  5:201410/1‑148 (MQ=255)
                                                                                                                              tacGGGTATAAAAAAACCCGCCGGGGCGGGTTTTGTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATAc                      >  8:396856/1‑148 (MQ=255)
                                                                                                                               acGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATAc                      >  5:381559/1‑147 (MQ=255)
                                                                                                                                cGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAg                   <  6:151362/149‑1 (MQ=255)
                                                                                                                                cGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACGCTGCAGCAGCTTCTGCTTTCTCTGAAGGATCAACCAGCTCGATGTAAGCCATCGGCGCGATGTCGCCTGCACGGAAGCCACACTTCAGAATACGa                    <  1:3198/148‑1 (MQ=255)
                                                                                                                                 gggTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAgt                  <  4:237351/149‑1 (MQ=255)
                                                                                                                                      taAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTaa              <  2:221126/148‑1 (MQ=255)
                                                                                                                                      taAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCAGCTTTCTCTGAACGATCAACCAGCTCGATGAAAGCCATCGGCGCGTTGTCGCCTGCTCGGAAGCGACACTTCAGAATACGAGTGTagc             >  3:38604/1‑147 (MQ=255)
                                                                                                                                        aaaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAccca           >  6:292186/1‑149 (MQ=255)
                                                                                                                                        aaaaaaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAacca           >  7:219244/1‑149 (MQ=255)
                                                                                                                                         aaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTTCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGATTACGAGTGTAaccac          >  1:123975/1‑149 (MQ=255)
                                                                                                                                         aaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAaccac          >  6:369464/1‑149 (MQ=255)
                                                                                                                                         aaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAaccac          >  4:14963/1‑149 (MQ=255)
                                                                                                                                         aaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTGCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTGCAGAATACGCGTGGAacccc          >  1:323802/1‑147 (MQ=255)
                                                                                                                                           aaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCCACCAGCTCGATGTACGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCCCACGTCAGAATACGAGTGTAACCCCCg        >  4:196900/1‑149 (MQ=255)
                                                                                                                                           aaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCg        >  6:360034/1‑149 (MQ=255)
                                                                                                                                            aaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGTTTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCgg       <  2:261517/149‑1 (MQ=255)
                                                                                                                                            aaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtctc                                                                                             >  3:90527/1‑63 (MQ=255)
                                                                                                                                            aaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtctc                                                                                             <  4:90527/63‑1 (MQ=255)
                                                                                                                                               ccgccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcgc                                                         >  4:131932/1‑96 (MQ=255)
                                                                                                                                               ccgccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcgc                                                         <  3:131932/96‑1 (MQ=255)
                                                                                                                                                 gccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACgg  >  1:252539/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGG  >  NC_000913/3439440‑3439733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: