Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,632,046	G→T	P164T (CCG→ACG)	guaA ←	GMP synthetase (glutamine aminotransferase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,632,046	0	G	T	100.0%	146.6 / NA	42	P164T (CCG→ACG)	guaA	GMP synthetase (glutamine aminotransferase)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/22);  total (20/22)

TGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTGCGCTG  >  NC_000913/2631906‑2632182                                                                                                                                             |                                                                                                                                         tGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCttt                                                                                                                                  >  8:85467/1‑149 (MQ=255)   gCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCg                                                                                                                                >  4:51463/1‑149 (MQ=255)        cacaGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGc                                                                                                                            <  6:53804/148‑1 (MQ=255)         acaGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTg                                                                                                                          >  7:80916/1‑149 (MQ=255)             ggCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGc                                                                                                                                        <  3:60122/131‑1 (MQ=255)             ggCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGc                                                                                                                                        >  4:60122/1‑131 (MQ=255)             ggCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTa                                                                                                                      <  8:80916/149‑1 (MQ=255)               cTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCt                                                                                                                           >  2:129131/1‑142 (MQ=255)               cTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCt                                                                                                                           <  1:129131/142‑1 (MQ=255)                ttCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACgg                                                                                                                   >  6:55189/1‑149 (MQ=255)                 tCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGt                                                                                                                  >  3:94049/1‑149 (MQ=255)                  cacaCTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCTTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTgg                                                                                                                         <  8:13706/141‑1 (MQ=255)                  cacaCTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTgg                                                                                                                         >  7:13706/1‑141 (MQ=255)                   acacTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGtg                                                                                                                 <  3:51463/148‑1 (MQ=255)                     acTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTgg                                                                                                                         <  5:65720/138‑1 (MQ=255)                     acTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTgg                                                                                                                         >  6:65720/1‑138 (MQ=255)                         gCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGtg                                                                                                                 <  3:87953/142‑1 (MQ=255)                         gCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGtg                                                                                                                 >  4:87953/1‑142 (MQ=255)                                      acaAAGCGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGc                                                                                             <  5:154501/149‑1 (MQ=255)                                      acaAACCGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGc                                                                                             <  5:156973/149‑1 (MQ=255)                                                  aGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATc                                                                                 <  5:55189/149‑1 (MQ=255)                                                     aTGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGcc                                                                              <  7:85467/149‑1 (MQ=255)                                                         gcATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGGGTTTTTCTTCGTTAGACATAATGTCAAACTTGCTGCTTTCGGTGCTGGCTACGGtgat                                                                                                               <  5:97058/112‑1 (MQ=255)                                                               ccTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATc                                                                    <  4:94049/149‑1 (MQ=255)                                                                              tCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGt                                                                            <  6:75983/126‑1 (MQ=255)                                                                              tCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGt                                                                            >  5:75983/1‑126 (MQ=255)                                                                                     cGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGtgat                                                                                                               <  7:140022/84‑1 (MQ=255)                                                                                     cGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGtgat                                                                                                               >  8:140022/1‑84 (MQ=255)                                                                                        gTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGt                                           >  4:128731/1‑149 (MQ=255)                                                                                         tGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTcgga                                                                                                      <  3:97855/89‑1 (MQ=255)                                                                                         tGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTcgga                                                                                                      >  4:97855/1‑89 (MQ=255)                                                                                            aaCTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGc                                       >  7:15919/1‑149 (MQ=255)                                                                                            aaCTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGc                                       >  5:103331/1‑149 (MQ=255)                                                                                                          gAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCtt                         <  6:103331/149‑1 (MQ=255)                                                                                                          gAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCtt                         <  8:15919/149‑1 (MQ=255)                                                                                                             gCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGt                                                                            <  7:100667/95‑1 (MQ=255)                                                                                                             gCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGt                                                                            >  8:100667/1‑95 (MQ=255)                                                                                                                               cATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTc                                                                                                         >  6:134439/1‑48 (MQ=255)                                                                                                                               cATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTc                                                                                                         <  5:134439/48‑1 (MQ=255)                                                                                                                                 tAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTGCGCTg  >  6:122633/1‑149 (MQ=255)                                                                                                                                  aaTGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAAc            <  6:66038/138‑1 (MQ=255)                                                                                                                                  aaTGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAAc            >  5:66038/1‑138 (MQ=255)                                                                                                                                             |                                                                                                                                         TGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTGCGCTG  >  NC_000913/2631906‑2632182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑