New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 951207 =135 (1.990)5 (0.080) 5/250 NT 3.8% intergenic (‑127/+65) pflA/pflB pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase/formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
?NC_000913 951233 = 125 (1.930)intergenic (‑153/+39) pflA/pflB pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase/formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
Rejected: Frequency below/above cutoff threshold.

Only 100 of 155 total aligned reads displayed.

AATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGA  >  NC_000913/951060‑951355
                                                                                                                                                   |                                                                                                                                                    
aaTGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTaaaa                                                                                                                                                      >  8:294338/1‑148 (MQ=255)
 aTGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTaaaaaa                                                                                                                                                    <  2:278370/149‑1 (MQ=255)
 aTGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTaaaa                                                                                                                                                      >  3:105346/1‑147 (MQ=255)
  tccgcagctgaccatccgtgtatctggctacgcagtacgtttcaactcgctgactaaagaacagcagcaggacgttattactcgtaccttcactcaatctatgtaattagatttgactgaaatcgtacagtaaaaagcgtacaataaa                                                                                                                                                    >  1:225696‑M1/146‑148 (MQ=255)
  tGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAg                                                                                                                                                   >  2:28010/1‑149 (MQ=255)
   ccgcagctgaccatccgtgtatctggctacgcagtacgtttcaactcgctgactaaagaacagcagcaggacgttattactcgtaccttcactcaatctatgtaattagatttgactgaaatcgtacagtaaaaagcgtacaatAAAgg                                                                                                                                                  <  5:338372‑M1/5‑1 (MQ=255)
    ctaCCAATAACTCACATTGCGGTGGTTCTCCAGATGGTGCCCTTCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAACGCTCTCGCGCTAAAATAGGc                                                                                                                                                 <  4:148129/147‑1 (MQ=255)
    cgcagctgaccatccgtgtatctggctacgcagtacgtttcaactcgctgactaaagaacagcagcaggacgttattactcgtaccttcactcaatctatgtaattagatttgactgaaatcgtacagtaaaaagcgtacaatAAAgg                                                                                                                                                  >  3:168862‑M1/144‑148 (MQ=255)
    cGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAgg                                                                                                                                                  <  7:195677/148‑1 (MQ=255)
     gACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGcc                                                                                                                                                >  2:100077/1‑149 (MQ=255)
        cAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCa                                                                                                                                             >  7:361376/1‑149 (MQ=255)
               tGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGt                                                                                                                                      >  1:348069/1‑149 (MQ=255)
                 aCATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTg                                                                                                                                     <  8:325556/148‑1 (MQ=255)
                  cATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGa                                                                                                                                   <  2:238639/149‑1 (MQ=255)
                  cATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGa                                                                                                                                   <  2:348069/149‑1 (MQ=255)
                  cATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGa                                                                                                                                   <  2:344512/149‑1 (MQ=255)
                      tatctggctacgcagtacgtttcaactcgctgactaaagaacagcagcaggacgttattactcgtaccttcactcaatctatgtaattagatttgactgaaatcgtacagtaaaaagcgtacaatAAAGGCCCCACTTTCGTGGAGCCt                                                                                                                               >  6:338372‑M1/126‑149 (MQ=255)
                        ggTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCtt                                                                                                                              >  5:245313/1‑148 (MQ=255)
                            tttCTCCAGATGTGGCCCATCTGAGGCCGTGTTTGTGCGCAGCTCGAAGTCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTAttt                                                                                                                         >  1:88005/1‑148 (MQ=255)
                            tttCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGCCAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCAGTTATTg                                                                                                                         >  1:82704/1‑149 (MQ=255)
                            tttCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTGCGTGGAGCCTTTATTg                                                                                                                         >  1:382129/1‑149 (MQ=255)
                                 ccAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGc                                                                                                                    >  5:8478/1‑149 (MQ=255)
                                        tgGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTa                                                                                                              <  7:234271/148‑1 (MQ=255)
                                         ggCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTa                                                                                                                            >  8:107302/1‑133 (MQ=255)
                                         ggCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTa                                                                                                                            <  7:107302/133‑1 (MQ=255)
                                         ggCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACt                                                                                                            >  2:375198/1‑149 (MQ=255)
                                          gCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTg                                                                                                           <  1:28010/149‑1 (MQ=255)
                                          gCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTg                                                                                                           >  4:171311/1‑149 (MQ=255)
                                           cccATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTa                                                                                                              >  1:317658/1‑145 (MQ=255)
                                           cccATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTg                                                                                                           >  7:109889/1‑148 (MQ=255)
                                            ccAGCTGAGGCCGGGTTGGGCCGCAGCTCGAAGGCTACGGCGAGTCTGTTTTGGCAGTCACCTTAAAGTAAAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTa                                                                                                         <  2:268303/149‑1 (MQ=255)
                                               tCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGa                                                                                                      >  5:80937/1‑149 (MQ=255)
                                               tCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGa                                                                                                      >  2:163750/1‑149 (MQ=255)
                                               tCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGa                                                                                                      >  1:369537/1‑149 (MQ=255)
                                                  gAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGAttt                                                                                                   >  2:383536/1‑149 (MQ=255)
                                                   aGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGAttt                                                                                                   >  8:160436/1‑148 (MQ=255)
                                                   aGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGAtt                                                                                                    <  2:376002/147‑1 (MQ=255)
                                                      actaaagaacagcagcaggacgttattactcgtaccttcactaaatctatgtaattctatttgacttaaatcgtacattaaacagcgtacaatAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCa                                                                                                 >  7:276582‑M1/94‑147 (MQ=255)
                                                        gtgtTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTc                                                                                              >  3:113684/1‑148 (MQ=255)
                                                           ttGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCaaa                                                                                           >  4:110798/1‑148 (MQ=255)
                                                             ggTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTAGCGCTAAAAAAGGCCCCACTTTCGTGTAGCCTTTATTTTACGCTTTTTACTGTAAGATTTCAGTCAAATc                                                                                         >  4:69894/1‑148 (MQ=255)
                                                                 cgcAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCTCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAAt                                                                                     <  7:45660/148‑1 (MQ=255)
                                                                 cgcAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAAtt                                                                                    <  3:301404/149‑1 (MQ=255)
                                                                 cgcAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAAt                                                                                     <  6:245313/148‑1 (MQ=255)
                                                                 cgcAGCTCGAAGGCTACGGCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAATGGCTCTCGCTCTAAAAAATGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAAt                                                                                     <  1:53247/148‑1 (MQ=255)
                                                                   cAGCTCGACGGCTACGGCGAGTCTGTGTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAACGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTAc                                                                                  <  5:278950/149‑1 (MQ=255)
                                                                    aGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACa                                                                                 >  5:267873/1‑149 (MQ=255)
                                                                      cTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATa                                                                               <  3:171311/149‑1 (MQ=255)
                                                                            ggCTACGTCGAGTCTGTTTT‑GCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATCGATTGAg                                                                        <  3:133217/149‑1 (MQ=255)
                                                                             gCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGAttt                                                                                                   >  6:266492/1‑122 (MQ=255)
                                                                             gCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAg                                                                        <  4:113684/149‑1 (MQ=255)
                                                                               tACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGAttt                                                                                                   <  2:366039/120‑1 (MQ=255)
                                                                                  gTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAg                                                                   <  6:267873/149‑1 (MQ=255)
                                                                                   tcgtaccttcactcaatctatgtaattagatttgactgaaatcgtacagtaaaaagcgtacaatAAAGGc                                                                                                                                                 >  7:37998‑M1/65‑70 (MQ=255)
                                                                                   tcgtaccttcactcaatctatgtaattagatttgactgaaatcgtacagtaaaaagcgtacaatAAAGGc                                                                                                                                                 <  8:37998‑M1/6‑1 (MQ=255)
                                                                                       gTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTAc                                                                                                             >  4:263272/1‑102 (MQ=255)
                                                                                       gTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTAc                                                                                                             <  3:263272/102‑1 (MQ=255)
                                                                                        tCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTAc                                                               >  7:282248/1‑147 (MQ=255)
                                                                                        tCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGa                                                             <  4:360932/149‑1 (MQ=255)
                                                                                          tGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAg                                                            >  4:369025/1‑148 (MQ=255)
                                                                                            ttttGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAg                                                            <  1:133756/146‑1 (MQ=255)
                                                                                            ttttGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGta                                                          <  3:369025/148‑1 (MQ=255)
                                                                                                ggCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGtaataa                                                      <  6:232114/148‑1 (MQ=255)
                                                                                                 gCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACg                                                    <  8:2612/149‑1 (MQ=255)
                                                                                                   aGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTc                                                  <  8:361376/149‑1 (MQ=255)
                                                                                                   aGTCACCTTAAAGTATAGATAGATGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTc                                                  <  8:100763/149‑1 (MQ=255)
                                                                                                     tCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCtt                                                                                                                  >  7:253243/1‑83 (MQ=255)
                                                                                                     tCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCtt                                                                                                                  <  8:253243/83‑1 (MQ=255)
                                                                                                         cTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgctg                                            <  3:69894/149‑1 (MQ=255)
                                                                                                         cTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCctgctg                                            >  8:46765/1‑149 (MQ=255)
                                                                                                              aGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGtt                                       >  2:95129/1‑149 (MQ=255)
                                                                                                                tataGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCt                                     >  1:36621/1‑149 (MQ=255)
                                                                                                                tataGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCt                                     >  5:261002/1‑149 (MQ=255)
                                                                                                                 tttgactgaaatcgtacagtaaaaagcgtacaatAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                    <  6:306546‑M1/115‑1 (MQ=255)
                                                                                                                  tagatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                    <  6:8478/148‑1 (MQ=255)
                                                                                                                  tagatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                    >  5:239383/1‑148 (MQ=255)
                                                                                                                  tagatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt                                    <  2:307499/148‑1 (MQ=255)
                                                                                                                   tgactgaaatcgtacagtaaaaagcgtacaatAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCttt                                   >  5:306546‑M1/33‑148 (MQ=255)
                                                                                                                    gatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGtaataa                                                      >  4:305743/1‑128 (MQ=255)
                                                                                                                    gatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTa                                  <  2:36621/148‑1 (MQ=255)
                                                                                                                    gatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTa                                  <  3:92150/148‑1 (MQ=255)
                                                                                                                    gatagCTGACAAAAAAGGATCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTa                                  <  7:294644/148‑1 (MQ=255)
                                                                                                                     atagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt                                <  7:34845/149‑1 (MQ=255)
                                                                                                                       agCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGCGCCTTTATTGTACGCTTTTTACTGTACGCTTTCAGTCAAATCTAATTACAGAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCGTTAgg                                >  1:242056/1‑146 (MQ=255)
                                                                                                                          tGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGTGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCg                           >  3:246935/1‑149 (MQ=255)
                                                                                                                            aCAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAg                         >  7:168010/1‑149 (MQ=255)
                                                                                                                              aaaaaaGTCTCTAGCTCTAAAAAAGGCCCCACTTTCGTGGAGCATTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGtt                       <  2:88005/149‑1 (MQ=255)
                                                                                                                              aaaaaaGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGtt                       >  7:326043/1‑149 (MQ=255)
                                                                                                                              aaaaaaGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGtt                       <  1:95129/149‑1 (MQ=255)
                                                                                                                              aaaaaaGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCATTTATTGTAAGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGtt                       <  2:82704/149‑1 (MQ=255)
                                                                                                                                 aaaGGCTCTCGCGCTAAAAAAGGCCCCCCTTTCGTGGAGCCTGTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGGGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGaa                    >  1:55634/1‑149 (MQ=255)
                                                                                                                                       tctcGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTAc              >  5:19195/1‑149 (MQ=255)
                                                                                                                                         tcGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTg            >  7:23900/1‑149 (MQ=255)
                                                                                                                                          cgcgCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGc           >  8:392153/1‑149 (MQ=255)
                                                                                                                                           gcgcTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGc           <  3:366278/148‑1 (MQ=255)
                                                                                                                                            cgcTAAAAAAGGCCCCAATTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTTTTCTTTAGTCAGCGAGTTGAAACGTACTGCGt         <  1:100077/149‑1 (MQ=255)
                                                                                                                                             gcTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGtt                       >  1:29967/1‑134 (MQ=255)
                                                                                                                                             gcTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGtt                       <  2:29967/134‑1 (MQ=255)
                                                                                                                                             gcTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCCAGtt                       >  8:350096/1‑134 (MQ=255)
                                                                                                                                                   AAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGc      <  5:151173‑M1/145‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGA  >  NC_000913/951060‑951355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.