| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| JC | NC_000913 | 756,291 | Δ11 bp | 36.9% | coding (385‑395/1767 nt) | sdhA → | succinate dehydrogenase, flavoprotein subunit |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 756290 | 41 (0.600) | 22 (0.340) | 22/250 | NT | 36.9% | coding (384/1767 nt) | sdhA | succinate dehydrogenase, flavoprotein subunit |
| ? | NC_000913 | 756302 = | 36 (0.560) | coding (396/1767 nt) | sdhA | succinate dehydrogenase, flavoprotein subunit | |||||
CGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTG > NC_000913/756155‑756445 | cgcgATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGgcac < 7:259637/149‑1 (MQ=255)cgcgATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGGCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGgcac < 5:366117/149‑1 (MQ=255) tgaccaggacgcgattgaatatatgtgtaaaaccgggccggaagcgattctggaactcgaacacatgggcctgccgttctcgcgtctcgatgatggtcgtatctatcaacgtccgtttggcggtcagtcgaaaaacttcggcggcacg > 7:368686‑M2/146‑148 (MQ=255) aaTATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGc < 5:145936/148‑1 (MQ=255) acgcgattgaatatatgtgtaaaaccgggccggaagcgattctggaactcgaacacatgggcctgccgttctcgcgtctcgatgatggtcgtatctatcaacgtccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCGgc < 1:78422‑M2/12‑1 (MQ=255) tgtgTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGgcag > 8:9425/1‑147 (MQ=255) tAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTg > 4:34463/1‑149 (MQ=255) aaaCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGa < 6:91143/148‑1 (MQ=255) cGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTa < 8:355454/149‑1 (MQ=255) tgtaaaaccgggcaggaagcgattctggaactcgaacacatgggcctgccgttctcgcgtctcgatgatgggcgtctctatcaacgtccgtttggcgggcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCg < 6:200707‑M2/29‑1 (MQ=255) gAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCa > 6:325286/1‑149 (MQ=255) gAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGc < 7:9425/148‑1 (MQ=255) gAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCt > 6:35749/1‑149 (MQ=255) aaCTCATGGGGCTGCGGTTCTGGGGTCTGGATGATGGTCGTATGTATCAAGGTCCGTTTGGGGGTCAGTCGATATCCTT‑‑‑‑‑‑‑‑‑G‑‑GGCGGCACGCACTGCGGCAGGAGGTGTCCGTACCGGTCACGCTCTGTTGCACACGCTTTATCAGCAGaa < 5:289351/149‑1 (MQ=255) acacATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCttt < 3:281116/149‑1 (MQ=255) ggaactcgaacacatgggcctgccgttctcgcgtctcgatgatggtcgtatctatcaacgtccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCCCACGCTTTAt > 3:270572‑M2/95‑149 (MQ=255) gaactcgaacacatgggcctgccgttctcgcgtctcgatgatggtcgtatctatcaacgtccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATc < 4:176493‑M2/56‑1 (MQ=255) caTGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTAt > 7:82982/1‑148 (MQ=255) caTGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATc < 5:337124/149‑1 (MQ=255) tGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATcag < 3:34463/149‑1 (MQ=255) tcgaacacatgggcctgccgttctcgcgtctcgatgatggtcgtatctatcaacgtccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTc < 4:246962‑M2/32‑1 (MQ=255) tcgaacacatgggcctgccgttctcgcgtctcgatgatggtcgtatctatcaacgtccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTc > 3:246962‑M2/90‑121 (MQ=255) gCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCACCGTCCGTTTTGCGGTCAGTCGAAAAACTACGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATcag > 6:97456/1‑146 (MQ=255) gaacacatgggcctgccgttctcgcgtctcgatgatggtcgtatctatcaacgtccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATcagcag > 8:197396‑M2/88‑148 (MQ=255) cTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGaa < 4:91203/149‑1 (MQ=255) tGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGcac > 2:11789/1‑131 (MQ=255) tGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGcac < 1:11789/131‑1 (MQ=255) tGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGcac < 1:5072/131‑1 (MQ=255) tGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGcac > 2:5072/1‑131 (MQ=255) tGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAAc > 3:300266/1‑149 (MQ=255) acatgggcctgccgttctcgcgtctcgatgatggtcgtatctatcaacgtccgtttggcggtcagtcgaaaaacttcggcgtcACGCACTGCGGCAGCCGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCt > 4:150982‑M2/84‑149 (MQ=255) tgccgttctcgcgtctcgatgatggtcgtatctatcaacgtccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAacca > 8:119561‑M2/75‑149 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAacca > 2:386090/1‑148 (MQ=255) tctcGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAaccaca < 8:375548/149‑1 (MQ=255) cgttctcgcgtctcgatgatggtcgtatctatcaacgtccgtttggcggtcagttgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAAc > 7:192970‑M2/72‑135 (MQ=255) cgttctcgcgtctcgatgatggtcgtatctatcaacgtccgtttggcggtcagttgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAAc < 8:192970‑M2/64‑1 (MQ=255) tcGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTc < 5:367522/102‑1 (MQ=255) tcGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTc > 6:367522/1‑102 (MQ=255) ttctcgcgtctcgatgatggtcgtatctatcaacgtccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTGCCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAaccacacca > 2:150303‑M2/70‑149 (MQ=255) gatgatGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAaccacacca < 5:35749/148‑1 (MQ=255) tatctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTcc > 2:36390/1‑148 (MQ=255) gatgatggtcgtatctatcaacgtccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTcc < 7:392380‑M2/91‑1 (MQ=255) tctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAg > 4:260420/1‑149 (MQ=255) tatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTg > 5:336173/1‑149 (MQ=255) tcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTg < 8:82982/147‑1 (MQ=255) gtcgtatctatcaacgtccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACTCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGt < 8:368686‑M2/98‑1 (MQ=255) tcgtatctatcaacgtccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTAt > 3:151291‑M2/50‑149 (MQ=255) gtatctatcaacgtccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCAGAGTGGTATgc > 2:8976‑M2/48‑149 (MQ=255) tctatcaacgtccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCCGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTCGTATGCGCt > 7:398217‑M2/45‑149 (MQ=255) acgtccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTGCCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTg < 1:150303‑M2/112‑1 (MQ=255) tccgtttggcggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTg < 3:57124‑M2/115‑1 (MQ=255) gAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGaa < 7:56478/90‑1 (MQ=255) gAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGaa > 8:56478/1‑90 (MQ=255) gAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAAc > 7:59916/1‑148 (MQ=255) aaaaaCTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCa < 5:325286/149‑1 (MQ=255) aaaaaCTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCa < 1:36390/149‑1 (MQ=255) cggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAg < 7:119561‑M2/124‑1 (MQ=255) tcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGAt > 1:152788‑M2/23‑149 (MQ=255) ttCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTgg > 6:382017/1‑133 (MQ=255) ttCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTgg < 5:382017/133‑1 (MQ=255) aaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCggt < 5:371606‑M2/135‑1 (MQ=255) cAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATTCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTggg > 6:289119/1‑149 (MQ=255) cAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGTATCTTGTTAAAAACCAGGATGTCGCGGTGGTggg < 5:97456/149‑1 (MQ=255) cggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTg > 3:96118‑M2/5‑148 (MQ=255) | CGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTG > NC_000913/756155‑756445 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |