Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,334,440 A→T 7.0% T196S (ACA→TCA)  cysB → N‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,334,4400AT 7.0% 147.0 / 2.1 57T196S (ACA→TCA) cysBN‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor
Reads supporting (aligned to +/- strand):  ref base A (22/30);  new base T (2/2);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GCCACAGAAGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTGGGGGTAGGGGTCATTGCCAG  >  NC_000913/1334296‑1334588
                                                                                                                                                |                                                                                                                                                    
gCCACAGAAGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACata                                                                                                                                                  <  7:183309/149‑1 (MQ=255)
 ccACAGAAGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGTCata                                                                                                                                                  >  1:181365/1‑148 (MQ=255)
 ccACAGAAGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGTCata                                                                                                                                                  >  1:186932/1‑148 (MQ=255)
      gAAGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCtt                                                                                                                                            <  2:148574/149‑1 (MQ=255)
        aGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGTCATATACCTTCg                                                                                                                                          <  2:186932/149‑1 (MQ=255)
        aGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGTCATATACCTTCg                                                                                                                                          <  2:181365/149‑1 (MQ=255)
                aTCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTAcc                                                                                                                                  >  7:394511/1‑149 (MQ=255)
                        gAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGtt                                                                                                                           >  4:330432/1‑148 (MQ=255)
                        gAAGATTTAGTGATGTTACAGTGCTACCACTTGAATCGGGCTATTGTAGTGAGTCCGGATCACCCGCTGGCATGCAAAAAAGCCATTACCATTGAAGAACTGTCGCAATATCCGTTGGTGACATATACCTTc                                                                                                                                           <  1:148433/132‑1 (MQ=255)
                         aaGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGtt                                                                                                                           >  4:169498/1‑147 (MQ=255)
                          aGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAg                                                                                                                        <  6:70648/149‑1 (MQ=255)
                             tttAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAAc                                                                                                                     >  8:200358/1‑149 (MQ=255)
                             tttAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAAc                                                                                                                     <  3:266116/149‑1 (MQ=255)
                              ttAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCAGGATAAAAAGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACATTCGGCTTTACCGGACGTTCAGAACt                                                                                                                    >  7:108224/1‑149 (MQ=255)
                                aGTGATGTTACCGTGCTACCTCTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTg                                                                                                                   <  7:128403/148‑1 (MQ=255)
                                 gggATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTgg                                                                                                                  <  1:59971/146‑1 (MQ=255)
                                 gTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGa                                                                                                                 <  2:383933/149‑1 (MQ=255)
                                 gTGATGTTACCGTGATACCACTGGAATCGGTGTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGa                                                                                                                 <  8:23206/149‑1 (MQ=255)
                                         aCCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCt                                                                                                         >  8:346307/1‑149 (MQ=255)
                                               cTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATc                                                                                                   <  8:394511/149‑1 (MQ=255)
                                                    aCTGGAATCGGCCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATAGAACAACTGGCGCAATATCCGTAGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTATAATcgcg                                                                                                >  8:177432/1‑147 (MQ=255)
                                                        gAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAgg                                                                                            <  1:140590/147‑1 (MQ=255)
                                                           tcaggCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTaa                                                                                       >  4:175873/4‑149 (MQ=255)
                                                           tcaggCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTaa                                                                                       >  4:181699/4‑149 (MQ=255)
                                                              ggCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAAcgc                                                                                    >  2:341474/1‑149 (MQ=255)
                                                                 tATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAAcgccgc                                                                                 >  1:106208/1‑149 (MQ=255)
                                                                   ttGTAGTCACTCCGGATCACCCGCTGGTAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAAcgcctc                                                                                 <  6:162843/147‑3 (MQ=255)
                                                                   ttGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAAcgccgc                                                                                 <  6:282070/147‑1 (MQ=255)
                                                                   ttGTAGTCACTCCGGATCACCCGCGGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAAAATCCGTAGGTGCAATATACCTTCGGCTATACCGTAATTTCAGAACTGGATACTGCCTATAATAGCGCAGGGTTAAcgccgcc                                                                                >  1:348533/1‑147 (MQ=255)
                                                                     gTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTa                                                                              <  7:200358/148‑1 (MQ=255)
                                                                      tAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATc                                                                            >  8:68995/1‑149 (MQ=255)
                                                                         tCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTTCCATAGATGAACTGGCGCAATTTACGTTGGTGACATATAGCTTCTGCTTTACCTGACGTTCAGAAGTGGATACTGCCTTTAATCGCGGTGGGTTAACGCCGCGTATCGtt                                                                         <  6:264620/149‑1 (MQ=255)
                                                                         tCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAgg                                                                                            >  4:337416/1‑130 (MQ=255)
                                                                         tCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAgg                                                                                            <  3:337416/130‑1 (MQ=255)
                                                                         tCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGtt                                                                         <  7:346307/149‑1 (MQ=255)
                                                                                        cGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGAt                                                          >  2:94793/1‑149 (MQ=255)
                                                                                        cGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGAt                                                          <  7:68995/149‑1 (MQ=255)
                                                                                             gcaggcaAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGa                                                     <  4:142216/149‑1 (MQ=255)
                                                                                                  caAAAAAGCCATTACCATTGAAGATCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCa                                                <  8:108224/149‑1 (MQ=255)
                                                                                                   aaaaaaGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCAt                                               >  6:335104/1‑149 (MQ=255)
                                                                                                        aGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTaaaa                                          <  3:193871/149‑1 (MQ=255)
                                                                                                           cATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACtt                                       <  1:341474/149‑1 (MQ=255)
                                                                                                            aTTACCATTGAAGAACTGGCGCAAAATCCGTTGGTGACATATAACTTAGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGTGTTAACGCCGCGTATCGTTTTCACGGCAACGGATCCTGACGTCATTAAAACTTa                                      >  4:201545/1‑149 (MQ=255)
                                                                                                                     gaagaaCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGt                              <  1:94793/148‑1 (MQ=255)
                                                                                                                      aagaaCTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGt                              >  5:3636/1‑147 (MQ=255)
                                                                                                                         aaCTGGCGCAATATCCGTTGGTGACATATTCCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTACTCGCGCAGGGTTAACGCCGAGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAggg                         <  7:142989/149‑1 (MQ=255)
                                                                                                                            tGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTg                      >  1:333474/1‑149 (MQ=255)
                                                                                                                              gcgcAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTggg                    <  3:169498/149‑1 (MQ=255)
                                                                                                                              gcgcAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTggg                    >  6:358342/1‑149 (MQ=255)
                                                                                                                              gcgcAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTggg                    >  6:296096/1‑149 (MQ=255)
                                                                                                                              gcgcAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTgg                     <  3:175873/148‑1 (MQ=255)
                                                                                                                              gcgcAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTgg                     <  3:181699/148‑1 (MQ=255)
                                                                                                                              gcgcAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTgg                     <  2:106208/148‑1 (MQ=255)
                                                                                                                                gcAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTggggg                  <  7:121643/149‑1 (MQ=255)
                                                                                                                                gcAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTggggg                  >  8:355914/1‑149 (MQ=255)
                                                                                                                                      tCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTGGGGGTAggg             <  7:355914/148‑1 (MQ=255)
                                                                                                                                         gTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTGGGGGTAGGGGTCa         >  6:37552/1‑149 (MQ=255)
                                                                                                                                                aCATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTGGGGGTAGGGGTCATTGCCAg  <  5:335104/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GCCACAGAAGCGCTGCATCTGTATGAAGATTTAGTGATGTTACCGTGCTACCACTGGAATCGGGCTATTGTAGTCACTCCGGATCACCCGCTGGCAGGCAAAAAAGCCATTACCATTGAAGAACTGGCGCAATATCCGTTGGTGACATATACCTTCGGCTTTACCGGACGTTCAGAACTGGATACTGCCTTTAATCGCGCAGGGTTAACGCCGCGTATCGTTTTCACGGCAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTGGGGGTAGGGGTCATTGCCAG  >  NC_000913/1334296‑1334588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑