Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 566,513 C→T 100% pseudogene (138/312 nt) peaD → pseudogene; DLP12 prophage; replication protein family;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913566,5130CT98.6% 7508.8 / 24.0 2604pseudogene (138/312 nt)peaDpseudogene; DLP12 prophage; replication protein family;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base C (22/18);  new base T (1358/1196);  total (1388/1216)
Fisher's exact test for biased strand distribution p-value = 8.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 2606 total aligned reads displayed.

CCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGT  >  NC_000913/566367‑566661
                                                                                                                                                  |                                                                                                                                                      
  tatggtttACGAGTATTGCCGGAAGCGAGGCCTGTATCCCGATGCCCAGACTTATACGTGGAAATCAAACGAGCGCTACTGGCTGGTTACCCACCTGTATCAGAACATGCGGGACAAATCGCTTACTGATGCGGAATTACGCCGTAAgg                                                                                                                                                    >  4:178519/8‑149 (MQ=255)
       tttACGAGTATTGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGccg                                                                                                                                                 >  3:347578/3‑145 (MQ=255)
           cGAGTATTGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGAt                                                                                                                                            <  4:57351/148‑1 (MQ=255)
            gAGTATTGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCGGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACTCCGTAAGGCCGCAGATGa                                                                                                                                          <  8:108082/149‑1 (MQ=255)
            gAGTATTGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGa                                                                                                                                          <  8:309719/149‑1 (MQ=255)
            gAGTATTGCCGGAAGCGACGCCTGTATCCGGATGCGGTTTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGGTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGa                                                                                                                                          <  7:203766/149‑1 (MQ=255)
               tATTGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCt                                                                                                                                     <  1:234598/149‑1 (MQ=255)
                 ttGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGa                                                                                                                                          >  3:2614/1‑144 (MQ=255)
                 ttGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCttg                                                                                                                                   >  1:154712/1‑147 (MQ=255)
                    ccGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCttgtcc                                                                                                                                <  2:19618/149‑6 (MQ=255)
                      ggAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCttgtccat                                                                                                                              <  1:207493/149‑8 (MQ=255)
                      ggAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCttgtccat                                                                                                                              <  3:32478/149‑8 (MQ=255)
                             aggCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGAt                                                                                                                                            >  1:308851/2‑130 (MQ=255)
                               gCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGc                                                                                                                                                <  8:190582/124‑1 (MQ=255)
                                    aatccggatccggAGTCTTATCCGTGGAAAGCACACGCGCACTACTGGCGGGTTACCAACCTGTAGCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAAt                                                                                                                <  2:375704/148‑1 (MQ=255)
                                        cGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTaa                                                                                                             <  5:48965/148‑1 (MQ=255)
                                            tGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCgtg                                                                                                        <  7:63518/149‑1 (MQ=255)
                                              cggagTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCgtggt                                                                                                      <  2:325085/147‑1 (MQ=255)
                                               ggagTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCgtggtg                                                                                                     <  5:131674/148‑1 (MQ=255)
                                               ggagTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCgtg                                                                                                        <  3:23002/145‑1 (MQ=255)
                                               ggagTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGAAGATGAGCTTGTCCATATGACTGCGAGAATTAACCgtggt                                                                                                      >  6:299234/2‑148 (MQ=255)
                                                 agTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTgag                                                                                                   <  6:213937/149‑1 (MQ=255)
                                                  gTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTgagg                                                                                                  <  7:119401/149‑2 (MQ=255)
                                                     ttATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCATCCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGa                                                                                               <  4:153434/149‑1 (MQ=255)
                                                     ttATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCtt                                                                                                                                    >  1:88731/1‑111 (MQ=255)
                                                     ttATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTgag                                                                                                   >  1:59318/1‑145 (MQ=255)
                                                     ttATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGa                                                                                               >  3:367958/1‑149 (MQ=255)
                                                     ttATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGGGGTGAGGCGa                                                                                               >  1:118796/1‑149 (MQ=255)
                                                       aTCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGca                                                                                                                                               >  4:291541/1‑100 (MQ=255)
                                                       aTCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCg                                                                                                <  8:156960/146‑1 (MQ=255)
                                                       aTCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGa                                                                                               <  2:305022/147‑1 (MQ=255)
                                                       aTCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGAGAGGCGAtc                                                                                             >  8:136568/1‑148 (MQ=255)
                                                         ccGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAAc                                                                                                            <  6:354394/132‑1 (MQ=255)
                                                           gTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGCTGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCt                                                                                          >  3:202659/1‑148 (MQ=255)
                                                           gTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGAtcc                                                                                            <  3:55458/146‑3 (MQ=255)
                                                           gTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCt                                                                                          >  1:336432/1‑148 (MQ=255)
                                                           gTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCt                                                                                          >  2:276195/1‑148 (MQ=255)
                                                            tGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTg                                                                                         >  8:219808/1‑148 (MQ=255)
                                                              gAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGaa                                                                                       <  3:287027/148‑1 (MQ=255)
                                                                 aTCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAg                                                                                   <  6:108298/149‑1 (MQ=255)
                                                                  tcaaaCGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGt                                                                                  <  8:74637/146‑1 (MQ=255)
                                                                    aaaCGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCTCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTa                                                                                 >  2:139749/2‑148 (MQ=255)
                                                                    aaaCGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTa                                                                                 >  8:36544/2‑148 (MQ=255)
                                                                     aaCGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTa                                                                                                              >  5:278019/1‑118 (MQ=255)
                                                                          gcACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAAc                                                                                                            <  6:5492/115‑1 (MQ=255)
                                                                          gcACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGCTCCCTGAACCAGTAAaacaac                                                                          >  1:101471/1‑149 (MQ=255)
                                                                           cactACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTaa                                                                                                                                                      <  1:367184/71‑1 (MQ=255)
                                                                           cactACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACt                                                                         <  3:174936/146‑1 (MQ=255)
                                                                             ctACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAaacaa                                                                           >  6:37848/2‑145 (MQ=255)
                                                                              tACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTcc                                                                      >  3:118241/1‑149 (MQ=255)
                                                                              tACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTcc                                                                      >  5:211312/1‑149 (MQ=255)
                                                                              tACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTcc                                                                      >  7:377907/1‑149 (MQ=255)
                                                                                cttgctggttACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGGGAGAATTAACCGTGTTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTg                                                                    <  5:161778/144‑1 (MQ=255)
                                                                                   gctggttACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGtca                                                                 <  1:184602/147‑3 (MQ=255)
                                                                                   gctggttACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGtca                                                                 >  2:115849/3‑147 (MQ=255)
                                                                                   gctggttACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGtca                                                                 >  6:161199/3‑147 (MQ=255)
                                                                                   gctggttACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGt                                                                   <  7:149972/145‑1 (MQ=255)
                                                                                       gttACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATggg                                                             >  7:88025/1‑149 (MQ=255)
                                                                                       gttACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATgg                                                              <  7:35055/148‑1 (MQ=255)
                                                                                          aCCAACCTGTATCAGAACATGCGGGCCAAGGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCATGAACCAGTAAAACAACTTCCTGACATGGGCgg                                                          >  2:347809/1‑149 (MQ=255)
                                                                                           ccAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCCTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCgg                                                          <  3:148559/148‑1 (MQ=255)
                                                                                             aaCCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAg                                                       >  5:323489/1‑149 (MQ=255)
                                                                                              acctGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCg                                                           <  4:98953/141‑1 (MQ=255)
                                                                                              acctGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGa                                                      >  5:302933/4‑149 (MQ=255)
                                                                                              acctGTATCAGAACATGCGGGCCAATGAGCTTACTGATGCGGAATTACGCAGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGa                                                      >  6:97818/4‑149 (MQ=255)
                                                                                               cctGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGAc                                                     >  5:188237/3‑149 (MQ=255)
                                                                                               cctGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGAc                                                     <  7:231735/147‑1 (MQ=255)
                                                                                                  ttataaGAACATGCCGCCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGCCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCTTGGTGAGTCGATCCCTTAACCAGTAAAACAACTTCCTGACATGCGCGGTAGACctc                                                  >  6:136386/6‑149 (MQ=255)
                                                                                                     tcAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATCACTGCGAGAATTAACCGTGGGGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTa                                                >  2:159517/2‑148 (MQ=255)
                                                                                                     tcAGAACATGAGGGCCAATGCGCTTACTGATGCGGAATTACTCCGTAAGGCCGCACATGAGCATGTCCATATGACTGCGAGAATTAACCGTGGTGAGCCGATCCCTGCACCAGTAAAACAACTTCCTGTCATCGGCGGTAGACCTCTa                                                >  6:187935/2‑148 (MQ=255)
                                                                                                      cAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTaaa                                              >  7:43098/1‑149 (MQ=255)
                                                                                                        gAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACTCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAAATAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATc                                            >  6:327974/1‑149 (MQ=255)
                                                                                                          aCATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGt                                          <  4:23298/149‑1 (MQ=255)
                                                                                                          aCATGCGGGCCAATGCGCTTACTGATGCGGAATTACGACGTAAGGCCGCAGATGAGCTTGTCCATTTGACTGCGAGAATTAAGCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTGATGGGGGGTAGACCTCTAAATCGt                                          <  5:359395/149‑1 (MQ=255)
                                                                                                           cATGCGGGCCAATGCGCTTACTGATGCGGACTAACGCCGTAAGGCCGCAGATGAGCTGGTCCATATGACTGCGAGACTTACCCGTGGTGAGGCGATCCCTGAACCAGTAAAACACCTTCCTGTCATGGGCGGTAGACCTCTAAATCgag                                         <  4:211577/149‑3 (MQ=255)
                                                                                                           cATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTg                                         <  7:317049/149‑1 (MQ=255)
                                                                                                               cGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGca                                       >  3:93063/1‑147 (MQ=255)
                                                                                                                  gCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGt                                                         >  8:332988/1‑126 (MQ=255)
                                                                                                                    cAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGctc                                >  2:251691/1‑149 (MQ=255)
                                                                                                                     aaTGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGctct                               >  7:173283/1‑149 (MQ=255)
                                                                                                                      aTGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTg                              >  5:245226/1‑149 (MQ=255)
                                                                                                                      aTGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTg                              <  4:264065/149‑1 (MQ=255)
                                                                                                                       tGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCTATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGGAGACCTCTAAATCGTGCACAGGCTCTgg                             <  1:283767/149‑1 (MQ=255)
                                                                                                                       tGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTgg                             <  7:288870/149‑1 (MQ=255)
                                                                                                                       tGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATAAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTgg                             >  6:24495/1‑149 (MQ=255)
                                                                                                                         cgcttactgATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGc                            <  6:76540/142‑1 (MQ=255)
                                                                                                                           cttactgATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATTGGCGTTAGACCTCTAAATCGTGCACAGTCTCTGGCGaa                         <  2:233816/145‑1 (MQ=255)
                                                                                                                           cttactgATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCg                           >  1:258878/5‑147 (MQ=255)
                                                                                                                           cttactgATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGaa                         <  4:321455/145‑1 (MQ=255)
                                                                                                                            ttactgATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCg                           >  7:259754/4‑146 (MQ=255)
                                                                                                                              actgATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTcc                                                                      >  8:283003/2‑101 (MQ=255)
                                                                                                                               ctgATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATc                     <  2:52506/149‑1 (MQ=255)
                                                                                                                                  atgcgGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCg                    <  8:48287/145‑1 (MQ=255)
                                                                                                                                   tgcgGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGaa                         <  5:75460/140‑1 (MQ=255)
                                                                                                                                   tgcgGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCg                    >  2:83356/2‑146 (MQ=255)
                                                                                                                                   tgcgGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAg                 <  4:36116/148‑1 (MQ=255)
                                                                                                                                    gcgGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGt                                          >  6:264197/1‑123 (MQ=255)
                                                                                                                                      ggAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAATACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGaaa              <  1:38727/149‑1 (MQ=255)
                                                                                                                                       gAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAAt             >  4:125756/1‑149 (MQ=255)
                                                                                                                                        aaTTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAAt             >  8:244922/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
CCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGT  >  NC_000913/566367‑566661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: