Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,987,104 T→A 7.9% intergenic (+28/‑432) yqeG → / → yqeH putative transporter/putative LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,987,1040TA 7.9% 234.4 / 8.1 89intergenic (+28/‑432)yqeG/yqeHputative transporter/putative LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (45/37);  new base A (3/4);  total (48/41)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

TTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACT  >  NC_000913/2986957‑2987251
                                                                                                                                                   |                                                                                                                                                   
ttCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCt                                                                                                                                                     <  2:215608/148‑1 (MQ=255)
   atcatcCCTACGCTGTCAACGTATCACATACCGGCGCGTCAACCCTGTCGTTCACTCGTAAATCTGCTTCCGCGGATCGGGGGTCTCCTGTGCGTCTCGGTAATTTTCTTTCGCTCATATCACTTGTGGATGGTTCAAAATTGCTACac                                                                                                                                                 <  4:29936/149‑2 (MQ=255)
   atcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACaa                                                                                                                                                 <  2:224711/149‑1 (MQ=255)
    tcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAt                                                                                                                                                >  4:201364/1‑149 (MQ=255)
          cTACGCTGTCAACGTATCTCATCCCGTCGCTTAAACCCTGGAGTTCGATAGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATTGTTCAAAATTGTAGCAATTTTGaa                                                                                                                                          <  7:47667/149‑1 (MQ=255)
          cTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGaa                                                                                                                                          <  4:325249/149‑1 (MQ=255)
              gCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccat                                                                                                                                      >  3:144170/1‑149 (MQ=255)
                tGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACATTTTTGAAccattc                                                                                                                                    >  3:252221/1‑149 (MQ=255)
                    aaCGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAATTCTGATTACGCTGATCGTGGGTATCATGTGCCTATCGGTAATGTTCGTTTGCTAATATCAGTTGTGGATGGTTCACAATTGCTACAATTTTGAAccattccatt                                                                                                                                >  8:388620/1‑149 (MQ=255)
                        tATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCAttttt                                                                                                                             >  5:136936/1‑148 (MQ=255)
                         aTCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGaa                                                                                                                                          >  5:270987/1‑134 (MQ=255)
                         aTCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGaa                                                                                                                                          <  6:270987/134‑1 (MQ=255)
                          tctcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCa                                                                                                                           <  3:303766/148‑1 (MQ=255)
                           ctcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCat                                                                                                                          >  3:67602/1‑148 (MQ=255)
                            tcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataa                                                                                                                        >  7:256008/1‑149 (MQ=255)
                             cATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataa                                                                                                                        >  5:94053/1‑148 (MQ=255)
                             cATCCCGGCGCTTAAACCCTCGCGTTCCATCGTAAATCTGATTACGCTGATCCTGGCTATACAGTGCGTAACGGTAATGTTCTTTATCTAATATCACATGTGGATGCTTCAAAATTGCTACAATTATGAACAAATCCATTTTtaataa                                                                                                                        >  4:334572/1‑148 (MQ=255)
                                  cGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCa                                                                                                                  >  3:202370/1‑149 (MQ=255)
                                       cTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGt                                                                                                             >  1:363413/1‑149 (MQ=255)
                                        ttAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATTTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCGATTGTTCATAATATTCATTTGtt                                                                                                            >  2:296856/1‑149 (MQ=255)
                                           aaCCCTGGCGTTCCATCTGAAATCTGATTACGCTGATCGTCGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGttat                                                                                                          <  7:382508/148‑1 (MQ=255)
                                            aCCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGttatta                                                                                                        >  4:271576/1‑149 (MQ=255)
                                              ccTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTattatt                                                                                                      >  6:140137/1‑149 (MQ=255)
                                               cTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAttt                                                                                                                                              >  5:201859/1‑108 (MQ=255)
                                               cTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAttt                                                                                                                                              <  6:201859/108‑1 (MQ=255)
                                               cTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTc                                                                                                     >  4:276719/1‑149 (MQ=255)
                                                        cATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTAt                                                                                             >  2:232217/1‑148 (MQ=255)
                                                        cATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTAt                                                                                             <  7:14225/148‑1 (MQ=255)
                                                                 tCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtctctc                                                                                   >  7:169874/1‑149 (MQ=255)
                                                                  cTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTtctctc                                                                                   <  1:301115/148‑1 (MQ=255)
                                                                    gATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGt                                                                                 >  8:59844/1‑148 (MQ=255)
                                                                     aTTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTc                                                                                <  3:13193/148‑1 (MQ=255)
                                                                      ttACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCa                                                                               >  4:80074/1‑148 (MQ=255)
                                                                        aCGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCata                                                                             <  2:363413/148‑1 (MQ=255)
                                                                          gCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAAAATTCATTTTTTATTATTCCACCATATCACTTCTATAGTAATATcc                                                                          >  2:386723/1‑149 (MQ=255)
                                                                              aTCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTa                                                                        <  1:390387/147‑1 (MQ=255)
                                                                              aTCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCACTTGTGGATGGTTCAAAATTGCTTCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTa                                                                        <  2:275425/147‑1 (MQ=255)
                                                                                 gtgtgtATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGtatt                                                                   <  4:247001/149‑1 (MQ=255)
                                                                                 gTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGtatt                                                                   <  6:136936/149‑1 (MQ=255)
                                                                                   ggtgagccTGTGCTTATCGTTAATGTTCTTTAGATAATATCAGTTTGGGATTGTTCAAACTTGCTACAATTTTGAACCATTCCATTTTTCATACTATTCATTTGTTATTATTCCACCCTATCTCTTCTCTAGTCATATCCTAGtattat                                                                 <  2:311972/143‑1 (MQ=255)
                                                                                      tATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCat                                                                              >  5:347589/1‑133 (MQ=255)
                                                                                      tATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCat                                                                              <  6:347589/133‑1 (MQ=255)
                                                                                          cTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTa                                                                        >  1:286996/1‑135 (MQ=255)
                                                                                          cTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTa                                                                        <  2:286996/135‑1 (MQ=255)
                                                                                          cTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagtta                                                           <  8:196838/148‑1 (MQ=255)
                                                                                           tgtgCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagttagt                                                         >  6:306395/1‑149 (MQ=255)
                                                                                           tgtgCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagttagt                                                         >  6:336784/1‑149 (MQ=255)
                                                                                             tgCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTGCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagttagtt                                                        >  4:22767/1‑148 (MQ=255)
                                                                                             tgCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagttagtt                                                        <  3:201364/148‑1 (MQ=255)
                                                                                              gCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTaa                                                      <  8:256008/149‑1 (MQ=255)
                                                                                                 tATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGttatta                                                                                                        <  6:229725/96‑1 (MQ=255)
                                                                                                 tATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGttatta                                                                                                        >  5:229725/1‑96 (MQ=255)
                                                                                                   tCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTACATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAacaca                                                 >  7:204316/1‑149 (MQ=255)
                                                                                                     ggTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAacacac                                                <  8:222494/148‑1 (MQ=255)
                                                                                                     ggTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAacacac                                                >  1:116897/1‑148 (MQ=255)
                                                                                                     ggTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAacacac                                                >  3:243271/1‑148 (MQ=255)
                                                                                                      gTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAAcacaca                                               >  1:283491/1‑148 (MQ=255)
                                                                                                       tAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTAt                                                                                             >  5:328759/1‑101 (MQ=255)
                                                                                                       tAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTAt                                                                                             <  6:328759/101‑1 (MQ=255)
                                                                                                       tAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACaaa                                             <  4:67602/149‑1 (MQ=255)
                                                                                                        aaTGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAAcacaca                                               >  1:177358/1‑146 (MQ=255)
                                                                                                         aTGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccatt                                                                                                                                     >  1:191224/1‑59 (MQ=255)
                                                                                                         aTGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccatt                                                                                                                                     <  2:191224/59‑1 (MQ=255)
                                                                                                          tGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACaaa                                             >  8:27768/1‑146 (MQ=255)
                                                                                                           gTTCTTTAGCTAATATCAGTTGTGGATGGTTCATAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAAAACAaaaa                                             >  3:364539/1‑145 (MQ=255)
                                                                                                           gTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACaaa                                             >  3:358957/1‑145 (MQ=255)
                                                                                                              cTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCt                                      <  4:144170/149‑1 (MQ=255)
                                                                                                                     tAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATc                               >  1:246041/1‑149 (MQ=255)
                                                                                                                          tCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTaaa                          <  5:140137/149‑1 (MQ=255)
                                                                                                                            aGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAAt                         >  3:82037/1‑148 (MQ=255)
                                                                                                                            aGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATa                        <  2:177358/149‑1 (MQ=255)
                                                                                                                            aGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAAAATTCATTTGTTATTATTCCACCCTATAACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAAt                         >  6:218613/1‑148 (MQ=255)
                                                                                                                             ggtgtgTATGGTTCAAAATTGCTACAATTTTGAAACATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATaa                       <  6:94053/147‑1 (MQ=255)
                                                                                                                             gTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATaa                       <  5:306395/149‑1 (MQ=255)
                                                                                                                               tgtgGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGa                     >  5:79568/1‑149 (MQ=255)
                                                                                                                                   gATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTAt                 >  5:52521/1‑149 (MQ=255)
                                                                                                                                   gATGGTTCAAAATTGCTACAATTTTGAACCATTACATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTa                  <  8:204316/148‑1 (MQ=255)
                                                                                                                                   aaTGGTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGGTCTGAACATCTTAAATaa                       >  1:281982/2‑143 (MQ=255)
                                                                                                                                   aaTGGTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGCTCTGAACATCTTAAATaa                       <  2:281982/142‑1 (MQ=255)
                                                                                                                                    aTGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTAtt                >  6:82765/1‑149 (MQ=255)
                                                                                                                                     tGGTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCt                                      <  3:210928/126‑1 (MQ=255)
                                                                                                                                     tGGTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCt                                      >  4:210928/1‑126 (MQ=255)
                                                                                                                                       gTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATaa                       <  6:100150/139‑1 (MQ=255)
                                                                                                                                       gTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATaa                       >  5:100150/1‑139 (MQ=255)
                                                                                                                                        ttCAAAATTGCTACAATTTTGAACCATTGCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTAttt               >  7:176345/1‑146 (MQ=255)
                                                                                                                                        ttCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAg            <  2:246041/149‑1 (MQ=255)
                                                                                                                                          cAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCTTCATATCCTAGTATTATCAGTTAGTTAAACACACATATAGATCTGAACATCTTAAATAAGACTATTTAAGa           <  2:283491/148‑1 (MQ=255)
                                                                                                                                                 gCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATaa    <  7:59844/148‑1 (MQ=255)
                                                                                                                                                  cTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAGTCTTATTTAAGATgttcaga   <  5:336784/148‑7 (MQ=255)
                                                                                                                                                  cTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAAc   <  6:79568/148‑1 (MQ=255)
                                                                                                                                                   tACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTTTTTAAGATGCATaagt  >  8:162806/1‑146 (MQ=255)
                                                                                                                                                   tACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACt  >  3:98806/1‑148 (MQ=255)
                                                                                                                                                   tACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACt  >  8:312740/1‑148 (MQ=255)
                                                                                                                                                   tACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACt  >  8:336978/1‑148 (MQ=255)
                                                                                                                                                   tACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTACACCATATAAATTCTCTCGTATTTTCCTAGTATTATCAGTTAGTTTAAAACACAAAGAGATCTGAACATATTTAATTAGAAATTTTAAGATGAATaaat  >  8:157337/1‑146 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACT  >  NC_000913/2986957‑2987251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: