Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,058,627 G→C 5.9% intergenic (‑217/+39) serA ← / ← rpiA D‑3‑phosphoglycerate dehydrogenase/ribose 5‑phosphate isomerase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,058,6270GC 5.9% 182.0 / 3.2 68intergenic (‑217/+39)serA/rpiAD‑3‑phosphoglycerate dehydrogenase/ribose 5‑phosphate isomerase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (28/36);  new base C (2/2);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

ATATTGCGGCAATATGAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCG  >  NC_000913/3058479‑3058774
                                                                                                                                                    |                                                                                                                                                   
atatTGCGGCAATATGAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATg                                                                                                                                                     <  4:335733/149‑1 (MQ=255)
   ttGCGGCAATATGAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGaa                                                                                                                                                   <  8:34287/148‑1 (MQ=255)
            tatGAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAAttttt                                                                                                                                         >  1:182315/1‑149 (MQ=255)
            tatGAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAAttttt                                                                                                                                         <  4:137498/149‑1 (MQ=255)
              tGAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAAtttttt                                                                                                                                        >  4:147043/1‑148 (MQ=255)
               gAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAAttttttt                                                                                                                                       >  2:368916/1‑148 (MQ=255)
                    tttGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACggg                                                                                                                                 >  5:113700/1‑149 (MQ=255)
                      tGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACgggg                                                                                                                                <  8:33166/148‑1 (MQ=255)
                      tGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACgggg                                                                                                                                <  5:170493/148‑1 (MQ=255)
                       gagTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACggggg                                                                                                                               <  3:347462/146‑1 (MQ=255)
                        cGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAg                                                                                                                             <  1:161410/149‑1 (MQ=255)
                        cGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAg                                                                                                                             <  4:325747/149‑1 (MQ=255)
                        cGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAg                                                                                                                             <  7:37429/149‑1 (MQ=255)
                                 gTTCAGGAAAAATCACGCAAAGGAAAATCTTGGAAGAATATTCAGGTTTAGACGATTTATATAGAGCATTCGACAATAGGCTCGTAAAAGCGTGAGACATGGCTCCAAATTTAATGAAGAGAAGTTTTTTAACGGGGGAGGTTCCACCg                                                                                                                    >  4:208513/1‑149 (MQ=255)
                                    gAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTc                                                                                                                  <  2:148426/148‑1 (MQ=255)
                                     aGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGAGTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCa                                                                                                                 <  7:367369/148‑1 (MQ=255)
                                     aGGAAAAATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCa                                                                                                                 <  7:181836/148‑1 (MQ=255)
                                        aaaTATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATc                                                                                                             <  8:341893/149‑1 (MQ=255)
                                        aaaTATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATc                                                                                                             <  2:48036/149‑1 (MQ=255)
                                           tatCACGCATAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCAtt                                                                                                          <  7:30146/149‑1 (MQ=255)
                                           tatCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTTGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCAtt                                                                                                          <  6:171369/149‑1 (MQ=255)
                                           tatCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTaa                                                                                                                                     >  2:25946/1‑122 (MQ=255)
                                                 gCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTccc                                                                                                                       <  8:227421/130‑1 (MQ=255)
                                                 gCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTccc                                                                                                                       >  7:227421/1‑130 (MQ=255)
                                                 gCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACaa                                                                                                    >  8:124743/1‑149 (MQ=255)
                                                      ggTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGtt                                                                                               <  3:147043/149‑1 (MQ=255)
                                                       gTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGttt                                                                                              <  7:124743/149‑1 (MQ=255)
                                                        tAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGtttt                                                                                             <  7:56562/149‑1 (MQ=255)
                                                          aTTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGa                                                                                           <  6:113700/149‑1 (MQ=255)
                                                                ggATGAATATGCTGGTTTAGAGTATGTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTGACCAAATTTTATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGAGCATTTCACAATGGTTTTGacac                                                                                        <  4:18015/146‑1 (MQ=255)
                                                                ggATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGacac                                                                                        <  2:182315/146‑1 (MQ=255)
                                                                 gATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGt                                                                                     >  7:98042/1‑148 (MQ=255)
                                                                 gATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTc                                                                                    <  4:267740/149‑1 (MQ=255)
                                                                  aTGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCa                                                                                   <  3:322793/149‑1 (MQ=255)
                                                                          gCTGGTTTAGAGGATTTATTTGGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGc                                                                            >  4:264303/1‑148 (MQ=255)
                                                                          gCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGc                                                                            >  4:305948/1‑148 (MQ=255)
                                                                              gTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAAt                                                                        <  8:379504/148‑1 (MQ=255)
                                                                                  agagGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGCGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAgc                                                                    <  2:250334/148‑1 (MQ=255)
                                                                                     ggATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCaa                                                                >  3:133602/1‑149 (MQ=255)
                                                                                           atttaGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTc                                                            <  4:21609/147‑1 (MQ=255)
                                                                                           atttaGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTc                                                            >  3:21609/1‑147 (MQ=255)
                                                                                               agagCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATCAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCgcgc                                                       >  1:100111/1‑148 (MQ=255)
                                                                                               agagCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATCAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCgcgc                                                       >  1:94997/1‑148 (MQ=255)
                                                                                                  gCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACg                                                   >  5:361370/1‑149 (MQ=255)
                                                                                                    aaTCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGt                                                 >  1:263626/1‑149 (MQ=255)
                                                                                                     aTCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGtt                                                <  1:345093/149‑1 (MQ=255)
                                                                                                        gACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAg                                              >  5:75755/1‑148 (MQ=255)
                                                                                                                ccTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGGCAATCAGCGCAACGTCTGCGCCACGGTTAGCAAACAAGc                                     >  7:237202/1‑149 (MQ=255)
                                                                                                                   ggTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTAAGGTGTTCCAATCAGCGCAACGTCCGCCCCACGGTAAGCAAACAAGCCaa                                  >  4:206984/1‑149 (MQ=255)
                                                                                                                     tAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACa                                <  8:324781/149‑1 (MQ=255)
                                                                                                                     tAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACa                                <  3:264303/149‑1 (MQ=255)
                                                                                                                     tAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACa                                <  3:305948/149‑1 (MQ=255)
                                                                                                                          gCGTGACACATGTCCCCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCACCGTCAGATCATTTCACAATGTTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCAGCGCCACGGTTAGCAAACAATCCcacacaggt                           >  6:332801/1‑144 (MQ=255)
                                                                                                                          gCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTcac                           <  8:98042/149‑1 (MQ=255)
                                                                                                                           cGTGACACATGTCAACAAATTTTATGAATAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACAGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTcacc                          <  3:206984/149‑1 (MQ=255)
                                                                                                                            gTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTcacca                         >  2:288638/1‑149 (MQ=255)
                                                                                                                             tGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGGCAATCAGCGCAACGTCTGCGCCACGGTTAGCAAACAAGCCAACAGTcaccac                        <  8:237202/149‑1 (MQ=255)
                                                                                                                                 acaTGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGc                      >  3:97268/1‑147 (MQ=255)
                                                                                                                                 acaTGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGc                      >  6:303484/1‑147 (MQ=255)
                                                                                                                                 acaTGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGc                      >  3:99010/1‑147 (MQ=255)
                                                                                                                                  caTGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAg                   <  4:325059/149‑1 (MQ=255)
                                                                                                                                  caTGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCACATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGACCGCGCCACGGTTAGAAAACAAGCCAACATTCACCCCCCCAg                   >  3:295128/1‑149 (MQ=255)
                                                                                                                                   aTGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAg                   >  4:10129/1‑148 (MQ=255)
                                                                                                                                        aCCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCg             >  5:142937/1‑149 (MQ=255)
                                                                                                                                         ccAAATTTAATCAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGc            <  2:94997/149‑1 (MQ=255)
                                                                                                                                         ccAAATTTAATCAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGc            <  2:100111/149‑1 (MQ=255)
                                                                                                                                          cAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCa           >  3:285392/1‑149 (MQ=255)
                                                                                                                                              tttAATGATTAGAATTTTTTTTACGGGTGAGGTTCCCCCGTCTGATCATTTAACAATGGTTTTGACACCGTCAGTTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCAAAGATTCACCACGCCATGAATCGCATTTa       <  4:295128/149‑1 (MQ=255)
                                                                                                                                                   tGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCg  >  3:318683/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATATTGCGGCAATATGAACGTTTGCGTCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCG  >  NC_000913/3058479‑3058774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: