Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,114,610 T→A 7.8% K1501N (AAA→AAT)  sslE ← putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,114,6100TA 7.8% 133.6 / 2.5 51K1501N (AAA→AAT) sslEputative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base T (20/27);  new base A (2/2);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

GCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCT  >  NC_000913/3114466‑3114749
                                                                                                                                                |                                                                                                                                           
gCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCg                                                                                                                                         >  6:81050/1‑149 (MQ=255)
  aGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGCCATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCgg                                                                                                                                        >  8:277694/1‑148 (MQ=255)
           gggCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAg                                                                                                                               <  7:195114/148‑1 (MQ=255)
           gggCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGt                                                                                                                              <  1:275026/149‑1 (MQ=255)
            ggCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGt                                                                                                                              >  7:284401/1‑148 (MQ=255)
             gCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGa                                                                                                                            >  8:7520/1‑149 (MQ=255)
              cAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGAc                                                                                                                           <  2:319739/149‑1 (MQ=255)
                 aTGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGAcgc                                                                                                                         >  5:194961/1‑148 (MQ=255)
                    tgtTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGc                                                                                                                     >  2:196918/1‑149 (MQ=255)
                    tgtTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGc                                                                                                                     <  7:7520/149‑1 (MQ=255)
                        aaCCCGCGGCGCCTTATTTCATGCCGGATCCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCgtg                                                                                                                  <  8:284401/148‑1 (MQ=255)
                         aCCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGTTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGtgtt                                                                                                                >  8:146347/1‑149 (MQ=255)
                         aCCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGtgtt                                                                                                                <  1:52687/149‑1 (MQ=255)
                         aCCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGtgtt                                                                                                                <  3:257075/149‑1 (MQ=255)
                         aCCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGtgtt                                                                                                                <  6:260205/149‑1 (MQ=255)
                             ggTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTaa                                                                                                            <  2:389399/149‑1 (MQ=255)
                                   ccTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGAc                                                                                                      <  3:83326/149‑1 (MQ=255)
                                    cTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACt                                                                                                     <  5:81050/149‑1 (MQ=255)
                                         cgtaggCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACGGCCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGCTCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCt                                                                                                 >  5:282688/6‑148 (MQ=38)
                                                  gATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGccc                                                                                       <  8:178649/149‑1 (MQ=255)
                                                      cggcgCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATAGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGa                                                                                   <  4:17448/149‑1 (MQ=255)
                                                       ggcgCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGATTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCg                                                                                    >  8:12192/1‑147 (MQ=255)
                                                       ggcgCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGATTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGaa                                                                                  >  8:17870/1‑149 (MQ=255)
                                                        gcgcGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAg                                                                                 >  6:91279/1‑149 (MQ=255)
                                                           cgAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCTAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTc                                                                              <  7:53747/149‑1 (MQ=255)
                                                                  cTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATctcgc                                                                       >  5:89901/1‑149 (MQ=255)
                                                                               cGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCAt                                                                            >  5:30842/1‑131 (MQ=255)
                                                                               cGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCAt                                                                            <  6:30842/131‑1 (MQ=255)
                                                                                  gCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTg                                                       <  3:135492/149‑1 (MQ=255)
                                                                                    ttACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTgg                                                      >  3:147319/1‑148 (MQ=255)
                                                                                      aCTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGAAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGt                                                     <  1:196918/147‑1 (MQ=255)
                                                                                             aGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTcg                                            >  4:308000/1‑149 (MQ=255)
                                                                                                 aTCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGcc                                        >  4:314133/1‑149 (MQ=255)
                                                                                                  tCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTa                                                                                                             <  1:344022/79‑1 (MQ=255)
                                                                                                  tCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTa                                                                                                             >  2:344022/1‑79 (MQ=255)
                                                                                                              tAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGATTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTc                           <  7:12192/149‑1 (MQ=255)
                                                                                                              tAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGATTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTc                           <  7:17870/149‑1 (MQ=255)
                                                                                                                  cTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTa                        >  7:365493/1‑148 (MQ=255)
                                                                                                                  cTGATTAATGGGTTCCGGTCCCTGTTCCGGTTGCGGCAGATCGCGTGAAGCGTGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGTAGCGCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCttta                       <  8:384931/149‑3 (MQ=255)
                                                                                                                    gATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTa                        >  7:84724/1‑146 (MQ=255)
                                                                                                                     cttAATGGTTTGCGGGCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGGTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCGGGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTaaa                      <  6:282688/146‑1 (MQ=255)
                                                                                                                     aTTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAg                     <  5:91279/148‑1 (MQ=255)
                                                                                                                        aaTGGTTTCCGGTCCCTGTTCCGGTTTCGGTAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGAGTCCATTTCTGAAAGCACt                 >  8:285295/1‑149 (MQ=255)
                                                                                                                          tGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTcc               >  1:195139/1‑149 (MQ=255)
                                                                                                                               tCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGaaa           <  3:350761/148‑1 (MQ=255)
                                                                                                                                 cGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGa         <  7:277694/148‑1 (MQ=255)
                                                                                                                                 cGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGAt        <  4:147319/149‑1 (MQ=255)
                                                                                                                                   gTCCCTGATCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATcc      <  6:194961/149‑1 (MQ=255)
                                                                                                                                       cTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCt  <  7:146347/149‑1 (MQ=255)
                                                                                                                                          ttCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATc       >  6:91709/1‑141 (MQ=255)
                                                                                                                                          ttCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATc       <  5:91709/141‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                           
GCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCT  >  NC_000913/3114466‑3114749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑