Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,015,333 A→T 6.0% intergenic (+19/+21) metE → / ← ysgA 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase/putative carboxymethylenebutenolidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,015,3330AT 6.0% 220.7 / 2.7 82intergenic (+19/+21)metE/ysgA5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase/putative carboxymethylenebutenolidase
Reads supporting (aligned to +/- strand):  ref base A (31/45);  new base T (2/3);  total (33/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACA  >  NC_000913/4015186‑4015481
                                                                                                                                                   |                                                                                                                                                    
gCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAAt                                                                                                                                                     <  4:330284/149‑1 (MQ=255)
gCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAAt                                                                                                                                                     <  8:12252/149‑1 (MQ=255)
    aaCGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATacca                                                                                                                                                 <  2:374624/149‑1 (MQ=255)
         aTTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAACTCCAAACCGGGTGGTAATACCACCCgg                                                                                                                                            >  2:237170/1‑149 (MQ=255)
          ttCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGt                                                                                                                                           >  7:63868/1‑149 (MQ=255)
                 agagCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTtct                                                                                                                                    <  8:63868/149‑1 (MQ=255)
                   agCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCa                                                                                                                                  >  7:172327/1‑149 (MQ=255)
                      gcCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATa                                                                                                                                                    <  4:171337/128‑1 (MQ=255)
                      gcCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATa                                                                                                                                                    >  3:171337/1‑128 (MQ=255)
                      gcCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTa                                                                                                                               <  2:246237/149‑1 (MQ=255)
                       ccTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTa                                                                                                                               >  6:11724/1‑148 (MQ=255)
                       ccTGTGGGTCAACCCGGACTGTGGCATGAAAACGCGCTGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGGCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTAc                                                                                                                              <  6:289849/149‑1 (MQ=255)
                        cTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTa                                                                                                                               >  7:195683/1‑147 (MQ=255)
                        cTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTa                                                                                                                               >  4:320891/1‑147 (MQ=255)
                        cTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTa                                                                                                                               >  3:199664/1‑147 (MQ=255)
                        cTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGGCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACa                                                                                                                             <  5:50155/149‑1 (MQ=255)
                        cTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGGGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACa                                                                                                                             <  7:291572/149‑1 (MQ=255)
                          gtgGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGc                                                                                                                           >  6:162921/1‑149 (MQ=255)
                            gggTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCGGttt                                                                                                                                         <  8:377005/133‑3 (MQ=255)
                             ggTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACa                                                                                                                             <  1:268462/144‑1 (MQ=255)
                             ggTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACa                                                                                                                             >  2:268462/1‑144 (MQ=255)
                             ggTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGa                                                                                                                         <  3:156517/148‑1 (MQ=255)
                             ggTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGAc                                                                                                                        <  6:369561/149‑1 (MQ=255)
                              gTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGAct                                                                                                                       <  3:44904/149‑1 (MQ=255)
                                  aCCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGActtctt                                                                                                                   >  3:368895/1‑149 (MQ=255)
                                   cccGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACttcttc                                                                                                                  >  5:54481/1‑149 (MQ=255)
                                   cccGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACttcttc                                                                                                                  >  3:140901/1‑149 (MQ=255)
                                    ccGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGTCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTcc                                                                                                                 <  4:368895/149‑1 (MQ=255)
                                    ccGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTcc                                                                                                                 <  1:122521/149‑1 (MQ=255)
                                    ccGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTcc                                                                                                                 >  8:126896/1‑149 (MQ=255)
                                     cGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCGGt                                                                                                                                           >  1:117997/1‑122 (MQ=255)
                                     cGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCGGt                                                                                                                                           <  2:117997/122‑1 (MQ=255)
                                      ggACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCcca                                                                                                               <  7:126896/149‑1 (MQ=255)
                                       gACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAACCCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCccac                                                                                                              <  6:21874/149‑1 (MQ=255)
                                       gACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTAGGGGGTAAAATCCAAACCGTGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCccac                                                                                                              <  6:366151/149‑1 (MQ=255)
                                               ccTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGCCTTCTTCCCACCATACTGc                                                                                                      >  4:154236/1‑149 (MQ=255)
                                                cTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCGGttt                                                                                                                                         >  5:40045/1‑111 (MQ=255)
                                                cTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTATTACCACCCGGttt                                                                                                                                         <  6:40045/113‑3 (MQ=255)
                                                cTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTc                                                                                                                                          <  5:23481/112‑1 (MQ=255)
                                                cTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTc                                                                                                                                          >  6:23481/1‑112 (MQ=255)
                                                cTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCt                                                                                                     >  7:89903/1‑149 (MQ=255)
                                                 tGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCt                                                                                                     >  2:126986/1‑148 (MQ=255)
                                                    aaaCGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTaaa                                                                                                 <  1:103758/149‑1 (MQ=255)
                                                     aaCGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAc                                                                                                >  4:185635/1‑149 (MQ=255)
                                                        gcgcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCAt                                                                                             <  5:11724/149‑1 (MQ=255)
                                                        gcgcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCAt                                                                                             >  5:341742/1‑149 (MQ=255)
                                                        gcgcgGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGAAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCAt                                                                                             >  6:291552/1‑149 (MQ=255)
                                                              cTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCATGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGc                                                                                       <  3:10203/149‑1 (MQ=255)
                                                                 gCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGAAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCa                                                                                      <  7:80479/147‑1 (MQ=255)
                                                                    aGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCCGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACg                                                                                  >  2:346350/1‑148 (MQ=255)
                                                                     gAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCt                                                                                <  7:80826/149‑1 (MQ=255)
                                                                       aaCCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTGTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTgc                                                                              <  2:268492/149‑1 (MQ=255)
                                                                            gcgcgGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTgccagc                                                                          >  1:52359/1‑148 (MQ=255)
                                                                                   aCTGGCGAACTTGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCAtcttct                                                                  <  6:341742/149‑1 (MQ=255)
                                                                                   aCTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCAtcttct                                                                  <  6:54481/149‑1 (MQ=255)
                                                                                       gCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTg                                                                 >  8:163687/1‑146 (MQ=255)
                                                                                                 tGCAGGCGGCGCAGAACTGGCGTCGGGGGTCAAATCAAAACCCGGTGGTAATACCACCCGGTGTATTCTCATTACAGCGACTTCTTCCCACCATACTGCTTATACCATTCCAGCATAGGCTGCCAGCCATCTTCTGCAGATGCGGCATg                                                    <  5:338441/149‑1 (MQ=255)
                                                                                                   cAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGa                                                   <  3:320891/148‑1 (MQ=255)
                                                                                                    aGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATa                                                 <  3:154236/149‑1 (MQ=255)
                                                                                                         gcgcAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCg                                            <  5:291552/149‑1 (MQ=255)
                                                                                                          cgcAGAACTTGCGTCGGGTGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCgg                                           <  7:396407/149‑1 (MQ=255)
                                                                                                           gcAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCGCCATACTGCTTAAACCATTCCCGCATACGCTGCCATCCATCTTCTGCAGATGCGGGATGATAGCTCggg                                          >  4:191859/1‑149 (MQ=255)
                                                                                                           gcAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCggg                                          >  4:56295/1‑149 (MQ=255)
                                                                                                             aGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGc                                         >  4:55996/1‑148 (MQ=255)
                                                                                                               aaCTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGAGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGTGCGAt                                      <  4:199664/149‑1 (MQ=255)
                                                                                                                     cGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCa                                 <  3:56295/148‑1 (MQ=255)
                                                                                                                     cGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCa                                 <  5:162921/148‑1 (MQ=255)
                                                                                                                     cGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAg                                <  8:62216/149‑1 (MQ=255)
                                                                                                                         gggggTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGt                             <  1:346350/148‑1 (MQ=255)
                                                                                                                            ggTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGaa                         >  5:199655/1‑149 (MQ=255)
                                                                                                                             gTAAAATCCAAACCGGGTGGTAATACCCCCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATACCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAat                        >  3:280645/1‑149 (MQ=255)
                                                                                                                             gTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAat                        <  2:238454/149‑1 (MQ=255)
                                                                                                                              tAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAatg                       <  6:199655/149‑1 (MQ=255)
                                                                                                                              tAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAatg                       <  7:163687/149‑1 (MQ=255)
                                                                                                                                      aaaCCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCg               >  2:156726/1‑149 (MQ=255)
                                                                                                                                        aCCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGAt                                                  <  5:116783/112‑1 (MQ=255)
                                                                                                                                        aCCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGAt                                                  >  6:116783/1‑112 (MQ=255)
                                                                                                                                            ggtggtAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTcc         <  4:231273/149‑1 (MQ=255)
                                                                                                                                              tggtAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCgg       <  5:38077/149‑1 (MQ=255)
                                                                                                                                                gtACTACCACCCGGTCTTTTCTCATTACCGCGACTTCTTCCCACCATACTGCTTAAACCATACCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGt     <  4:280645/149‑1 (MQ=255)
                                                                                                                                                gtAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTTCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCggg      <  2:170423/148‑1 (MQ=255)
                                                                                                                                                gtAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATACATGCCCGGCGTCCGGGt     <  8:89903/149‑1 (MQ=255)
                                                                                                                                                   aTACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTaca  <  3:55996/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACA  >  NC_000913/4015186‑4015481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: