Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,457,300 A→T 6.9% intergenic (+142/+14) mpl → / ← yjgA UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase/ribosome‑associated UPF0307 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,457,3000AT 6.9% 154.1 / 2.3 58intergenic (+142/+14)mpl/yjgAUDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase/ribosome‑associated UPF0307 family protein
Reads supporting (aligned to +/- strand):  ref base A (31/23);  new base T (2/2);  total (33/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGC  >  NC_000913/4457152‑4457446
                                                                                                                                                    |                                                                                                                                                  
gcAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTcaca                                                                                                                                                    <  1:269974/149‑1 (MQ=255)
  aGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACAt                                                                                                                                                   >  8:96604/1‑148 (MQ=255)
      aTTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATcaac                                                                                                                                               >  1:208566/1‑148 (MQ=255)
       ttCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATcaacaa                                                                                                                                             >  3:294518/1‑149 (MQ=255)
              ttAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAAt                                                                                                                                      >  5:234561/1‑149 (MQ=255)
                      atatAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTc                                                                                                                              <  7:167824/149‑1 (MQ=255)
                            tATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTtctc                                                                                                                        >  5:113155/1‑149 (MQ=255)
                                cGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCt                                                                                                                    <  3:257290/149‑1 (MQ=255)
                                    ggCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTc                                                                                                                              <  8:260317/135‑1 (MQ=255)
                                    ggCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTc                                                                                                                              >  7:260317/1‑135 (MQ=255)
                                       cGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCg                                                                                                                             >  3:197350/1‑133 (MQ=255)
                                       cGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCg                                                                                                                             <  4:197350/133‑1 (MQ=255)
                                       cGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTcgc                                                                                                             >  7:304538/1‑149 (MQ=255)
                                       cGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTcgc                                                                                                             <  5:13832/149‑1 (MQ=255)
                                              ggCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATa                                                                                                      >  6:195420/1‑149 (MQ=255)
                                               gCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGaaaa                                                                                                                                       <  7:288898/115‑1 (MQ=255)
                                               gCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGaaaa                                                                                                                                       >  8:288898/1‑115 (MQ=255)
                                                  ttCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACAGCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTg                                                                                                   >  4:336973/1‑148 (MQ=255)
                                                       gccgcATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAAt                                                                                             >  5:305126/1‑149 (MQ=255)
                                                          gcATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTg                                                                                          <  8:347151/149‑1 (MQ=255)
                                                                       gAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGAGTTc                                                                             >  2:359962/1‑149 (MQ=255)
                                                                           aTAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTcggcg                                                                         <  5:176341/149‑1 (MQ=255)
                                                                            tAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCtt                                                                                                                               >  7:338290/1‑94 (MQ=255)
                                                                            tAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCtt                                                                                                                               <  8:338290/94‑1 (MQ=255)
                                                                              gTGCCTTATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCggcggc                                                                       <  6:17359/148‑1 (MQ=255)
                                                                                  cTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTgg                                                                                         <  7:14935/126‑1 (MQ=255)
                                                                                  cTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTgg                                                                                         >  8:14935/1‑126 (MQ=255)
                                                                                  cTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTAtt                                                                  <  3:149337/149‑1 (MQ=255)
                                                                                      tGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCct                                                              >  6:287616/1‑149 (MQ=255)
                                                                                         gACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCcttct                                                           >  8:176145/1‑149 (MQ=255)
                                                                                          aCGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCcttctt                                                          <  4:294518/149‑1 (MQ=255)
                                                                                              ttGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTtctttctc                                                      >  1:372274/1‑149 (MQ=255)
                                                                                                 aTGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTtctttctcttt                                                   <  7:176145/149‑1 (MQ=255)
                                                                                                    cGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCtctttctt                                                >  7:208424/1‑149 (MQ=255)
                                                                                                        ttATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTcgcg                                            >  6:209756/1‑149 (MQ=255)
                                                                                                          aTCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGtt                                          >  1:269366/1‑149 (MQ=255)
                                                                                                             aGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCg                                       >  7:271393/1‑149 (MQ=255)
                                                                                                                  tACAGGTGCACCGTATCCGGCAAACCATACTCACTTCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGag                                    >  2:285279/1‑146 (MQ=255)
                                                                                                                  tACAGGTGCACCGTATCCGGCAAACCATACTCACTTCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGAt                                    >  2:290549/1‑147 (MQ=255)
                                                                                                                  tACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGAt                                    >  8:345160/1‑147 (MQ=255)
                                                                                                                    cAGGTGCACCGTATCCGGCAAACCATACTCACTTCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCaga                                <  1:290549/149‑1 (MQ=255)
                                                                                                                    cAGGTGCACCGTATCCGGCAAACCATACTCACTTCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCaga                                <  1:285279/149‑1 (MQ=255)
                                                                                                                    cAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCaga                                <  6:234561/149‑1 (MQ=255)
                                                                                                                    cAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCa                                  <  2:208566/147‑1 (MQ=255)
                                                                                                                      ggTGCACCGTATCAGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGAGTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCagag                               >  2:347195/1‑148 (MQ=255)
                                                                                                                           aCCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCa                         <  5:195420/149‑1 (MQ=255)
                                                                                                                            ccGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAg                        <  7:345160/149‑1 (MQ=255)
                                                                                                                            ccGTATCCGGCAAACCATACTCACATCAACAACGAAAATTAACCTTCGTTCTCCGCTAACTCGCGCAGAGACTGGAAAATCTGGCGTGCGGATTTAGGCGGCTTATTCCCTTATTTCTCTTTATTAGCGTTGATGATCAGAGTACGCAg                        >  3:60590/1‑149 (MQ=255)
                                                                                                                             cttATCCGGCAATCCATTCTCACATCAACAAAGAAAATTACTCTTCGTTCTCCGCTAACTCGCGCAGATACTGTAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGt                       <  8:304538/147‑1 (MQ=255)
                                                                                                                             cGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATc                                   <  6:289632/137‑1 (MQ=255)
                                                                                                                             cGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATc                                   >  5:289632/1‑137 (MQ=255)
                                                                                                                             cGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGt                       >  7:15939/1‑149 (MQ=255)
                                                                                                                                 tCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGc                                                                                        <  6:27607/80‑1 (MQ=255)
                                                                                                                                 tCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGc                                                                                        >  5:27607/1‑80 (MQ=255)
                                                                                                                                  ccGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCt                   >  8:2619/1‑148 (MQ=255)
                                                                                                                                   cGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTg                  <  7:2619/148‑1 (MQ=255)
                                                                                                                                          ccATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCgg          >  7:318097/1‑149 (MQ=255)
                                                                                                                                                tCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCa                                  >  2:311260/1‑119 (MQ=255)
                                                                                                                                                tCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCa                                  <  1:311260/119‑1 (MQ=255)
                                                                                                                                                  acaTCCCCAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACGGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGc  >  6:89129/1‑149 (MQ=255)
                                                                                                                                                  acaTCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCgg    <  5:209756/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGC  >  NC_000913/4457152‑4457446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑