Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 578,196 A→C 39.4% A30A (GCA→GCC rzpD → DLP12 prophage; putative murein endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913578,1960AC39.4% 80.0 / 66.0 76A30A (GCA→GCCrzpDDLP12 prophage; putative murein endopeptidase
Reads supporting (aligned to +/- strand):  ref base A (22/24);  new base C (17/13);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCTGAAAA  >  NC_000913/578050‑578342
                                                                                                                                                   |                                                                                                                                                  
gAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAAt                                                                                                                                                  <  8:274063/149‑1 (MQ=255)
  aaGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCAGTCAATCATTACCGTGATAACGCAATCg                                                                                                                                                >  7:220323/1‑149 (MQ=255)
     gCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATAATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGcc                                                                                                                                              >  4:317541/1‑148 (MQ=255)
         gatgaCTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGAATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACa                                                                                                                                          >  6:187840/1‑148 (MQ=255)
          atgaCTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACaaa                                                                                                                                        <  6:123472/149‑1 (MQ=255)
                 gtcgtGAGATTGAGCGTGAAGTCTCTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGATCAATAATTAACTTGATAAATCAATAGCCTACAAAGTCAAgc                                                                                                                                 >  7:353498/1‑149 (MQ=255)
                          ttGAGCGTGAAGTCTGTTTGTGCCGCCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTCATTATCTGCATCATCGTCAGCCTGTCATGGGCGGGCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCACCGCGACaaa                                                                                                                         >  5:4801/1‑148 (MQ=37)
                                 tGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGc                                                                                                                    >  3:255292/1‑146 (MQ=255)
                                   aaGTCTGTTTGTTGGGGCAACAGGGAGCAGAGTCACCGCGATTATATACGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCaga                                                                                                                <  6:105064/148‑1 (MQ=255)
                                     gtattgttGTGGGGGCAACAGTGAGCAGAGTAACCGAGAGTATATCCGCTCTGATTATCTGCATCATCTTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGaa                                                                                                             <  2:199497/143‑1 (MQ=25)
                                                ggggCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGc                                                                                                  <  4:255292/149‑1 (MQ=255)
                                                   gCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGaa                                                                                               <  5:148594/149‑1 (MQ=255)
                                                    gatgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGCTTATCTGCATCATCGTCTGCCTGTCATGGTCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAAc                                                                                              >  2:272482/7‑149 (MQ=11)
                                                     atgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGc                                                                                                                    <  3:203957/121‑1 (MQ=11)
                                                     atgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCa                                                                                                                  >  4:315251/6‑128 (MQ=11)
                                                     atgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCa                                                                                                                  <  3:315251/123‑1 (MQ=11)
                                                      tgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCCTCGTCTGCCTGTCATGGGCGGTTTATCATTCCCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAAcgc                                                                                            >  3:300436/5‑149 (MQ=11)
                                                       gtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCaga                                                                                                                >  1:183910/4‑128 (MQ=11)
                                                       gtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCaga                                                                                                                <  2:183910/125‑1 (MQ=11)
                                                       gtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAAc                                                                                                            <  1:217212/129‑1 (MQ=11)
                                                       gtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAAc                                                                                                            >  2:217212/4‑132 (MQ=11)
                                                       cAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAAcgcg                                                                                           >  7:344002/1‑149 (MQ=255)
                                                        tatGAGCAGAGTCACCGCGATTATCTCCGCTCTGTTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATACCGGCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAAcgcg                                                                                           <  6:191777/146‑1 (MQ=11)
                                                        tatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCa                                                                                                                  >  6:105395/3‑125 (MQ=11)
                                                        tatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCa                                                                                                                  <  5:105395/123‑1 (MQ=11)
                                                         gTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCgg                                                                                          >  6:174608/1‑148 (MQ=255)
                                                          tGAGCAGAGTCACCGCGATTATCTCCGCTCTGTTTATCTGCATCATCGTCTGCCTGTCATTGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGc                                                                                         <  6:109236/148‑1 (MQ=11)
                                                          tGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCagag                                                                                                               >  3:311728/1‑126 (MQ=11)
                                                          tGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCagag                                                                                                               <  4:311728/126‑1 (MQ=11)
                                                          tGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCtg                                                                                                    >  3:230302/1‑136 (MQ=11)
                                                          tGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCtg                                                                                                    <  4:230302/137‑2 (MQ=11)
                                                           gAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGa                                                                                                         <  7:210068/131‑1 (MQ=11)
                                                           gAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGa                                                                                                         >  8:210068/1‑131 (MQ=11)
                                                           gAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCtg                                                                                                    <  2:218203/136‑2 (MQ=11)
                                                            aGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCagag                                                                                                               <  6:6182/124‑1 (MQ=11)
                                                            aGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCagag                                                                                                               >  5:6182/1‑124 (MQ=11)
                                                            aGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCAGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAAt                                                                                      <  8:220323/149‑1 (MQ=255)
                                                             gCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAgcgc                                                                                                                               >  8:65908/1‑107 (MQ=11)
                                                               agagTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCTGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGc                                                                                                  <  6:91822/134‑1 (MQ=255)
                                                               agagTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGc                                                                                                  >  5:91822/1‑134 (MQ=255)
                                                                      ccGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATg                                                                            >  2:96416/1‑149 (MQ=11)
                                                                       cgcgATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATg                                                                            >  8:169899/1‑148 (MQ=255)
                                                                                ctCCGATCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATGATTACCGAGATAACGACATTACCTACAACGCCCAGCGCGCCAAAAATGCAAGAGAACTGAAGCTGGAGCACGCGGCAATTACTGACATGAAGATGCGt                                                                   >  6:236726/2‑148 (MQ=255)
                                                                                    gCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCTAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCg                                                              <  8:103696/149‑1 (MQ=255)
                                                                                        tGATTATCTGCATCATCGTCACCCTGTCATGGGCGGTCAATCATTACCGTGATAACGGATTCGCCTACAATGTCCAGCGCGACAAAAATGCCAGAGATCTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGAt                                                          <  6:4801/149‑1 (MQ=17)
                                                                                          gtgaTCTTCATCATCGTCTGCCTGTCATGGGCGGGTAATCATTACCGTGATAACGCCATTACCTAAAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGt                                                        <  4:188766/146‑1 (MQ=11)
                                                                                          aTTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGt                                                        >  1:106550/1‑149 (MQ=255)
                                                                                                  catcatCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTtgct                                                    <  5:174608/145‑1 (MQ=255)
                                                                                                  catcatCGTCAGCATGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTgcgc                                                >  8:153312/1‑149 (MQ=255)
                                                                                                   atcatcGTCAGCCTGTCATGGGCTGTAAATCATTACCGTGATAAAGCCATTACATACAAAGCCCATCGCGACAAAAATGCAAGAGAACTGAACCTGGCTAACACGGCAATAACTGACATGCAGATGCGTCAGAGTGATGTTGCTGCGCt                                               >  8:249689/1‑149 (MQ=255)
                                                                                                    tcatcGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTc                                              >  7:305095/1‑149 (MQ=255)
                                                                                                     catcGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCACAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCTGCGTGATGTTGCTGCGCTc                                              >  6:382667/1‑148 (MQ=11)
                                                                                                     catcGTCTGCCTGTCATGGGCTGTTAATCATTAACGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGATAACTGAAGCTGTCTAACTAGGCAATTACTGACATTCAGATGAGTCAGAGTGATGTTTCTGAGCTc                                              >  6:67882/1‑148 (MQ=255)
                                                                                                      atcGGCAGCCTGTCATGGGCGGTCTATCTTTACCGTGATCTGTCATTCGCGTACTTAGTCCAGCGCGTGATATATGCCAGTGAACTGATGCTAGCGATCGGGGCTATTACTGACATGCATATGCGTCAGCGTGATGTTGCTGCGCTCgt                                            <  6:71059/149‑2 (MQ=12)
                                                                                                       tcGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGAt                                           <  6:133371/149‑1 (MQ=255)
                                                                                                             gCCTGTCATGGGCGGTCAATCATTAGCGTGATAACGCAATCGCCTACAAAGTCCTGCGCGACAAAACTGCCAGAGCACTGAAGCTAGCGATCGCGGCAATTACTGACCTGCAGATGCGGCAGCGTGATGTTGCTGCGCTCGATGCaaaa                                     <  2:278439/149‑1 (MQ=17)
                                                                                                                  tCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAg                                                                         >  6:347098/1‑108 (MQ=255)
                                                                                                                  tCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAg                                                                         <  5:347098/108‑1 (MQ=255)
                                                                                                                  tCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATac                                  <  5:187840/147‑1 (MQ=255)
                                                                                                                       ggatgttaaTCATTACCGTGATAACGCAATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAAATGAAGCTTGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAg                            <  1:97848/141‑1 (MQ=11)
                                                                                                                       ggCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAg                            <  2:106550/148‑1 (MQ=255)
                                                                                                                        gCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAg                            >  2:314392/1‑147 (MQ=255)
                                                                                                                          ggTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGAGACAAAAATGCCAGAGACCTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGt                        <  5:341392/149‑1 (MQ=255)
                                                                                                                            tCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTa                      <  7:352744/149‑1 (MQ=255)
                                                                                                                            tCAATCATTACCGTGATAACGCAATCGACTACAAAGTCCAGCGCGAAAAAAATGCCAGAGAACTGAAGATAGCGAACGCGGCAATTACTGACATGAAGATGCGTCAGCTTGATGTTGCTGCTCTCGATGCAAAATACACGaa                             >  8:217084/1‑142 (MQ=14)
                                                                                                                                  aTTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAATCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGAt                <  3:317541/149‑1 (MQ=255)
                                                                                                                                  aTTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGa                 <  7:169899/148‑1 (MQ=255)
                                                                                                                                   ttACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGAAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATg               >  4:359584/1‑149 (MQ=255)
                                                                                                                                   ttACAGTGATAACGACAATACATACAAATCCCAGCGAGACAAAAAAGCCAGAGAACTGAAGCTGGCGAACGAGGCAATTACTGACAAGCAGATGCGTCAGAGAGAAGTTGCTGCGATAGATGAAAAATACACGAAGGAGTTAGCTGAt                >  4:314430/1‑148 (MQ=2)
                                                                                                                                    tACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGc              >  6:149577/1‑149 (MQ=255)
                                                                                                                                        gTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGaaa          <  8:305095/149‑1 (MQ=255)
                                                                                                                                         tGATAACGCCATTACATACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTCGCGAACGCGGCAATTACTGACATGAAGATGCGTCAGCGTGATCTTCCTGCCCTCGAAGCAAAATCCACGAAGCAGTAAGCTGATGATAAAg         >  4:283889/1‑149 (MQ=11)
                                                                                                                                           aTAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCt       >  2:165247/1‑149 (MQ=255)
                                                                                                                                               cGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTa                      >  2:303938/1‑130 (MQ=255)
                                                                                                                                               cGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTa                      <  1:303938/130‑1 (MQ=255)
                                                                                                                                                gCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCTGaaaa  >  7:225440/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCTGAAAA  >  NC_000913/578050‑578342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: