Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 755,646 C→A Y29* (TAC→TAA sdhD → succinate dehydrogenase, membrane subunit, binds cytochrome b556

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913755,6460CA98.9% 307.2 / NA 89Y29* (TAC→TAAsdhDsuccinate dehydrogenase, membrane subunit, binds cytochrome b556
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (43/45);  minor base T (0/1);  total (43/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGG  >  NC_000913/755503‑755790
                                                                                                                                               |                                                                                                                                                
cGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAaatcat                                                                                                                                             >  7:226463/1‑149 (MQ=255)
cGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAaatcat                                                                                                                                             >  3:253285/1‑149 (MQ=255)
  ccAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCAtt                                                                                                                                            >  3:50312/1‑148 (MQ=255)
            cTTTGTTATTACTGTCGTGCTTTCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATgg                                                                                                                                     <  7:30600/145‑1 (MQ=255)
            cTTTGTTATTACTGTCGTGCTTTCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTc                                                                                                                                   <  8:257899/147‑1 (MQ=255)
               tGTTATTACTGTCGTGCTTTCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGt                                                                                                                                <  2:228983/147‑1 (MQ=255)
                    ttaCTGTCGTGCTTTCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCg                                                                                                                         >  1:356424/1‑149 (MQ=255)
                    ttaCTGTCGTGCTTTCACTTCTCGCAGGAGTCCTCGTATGGGAACGAACTCCTCCGGATTAGGACTCATTGGCGTACATGATTTCATCCTCGTTATCGCTTCCGCTATCGTCCTGAAGCTCTATATCTTTTATATGGTCGGTGTTTTc                                                                                                                          <  2:171804/148‑1 (MQ=255)
                            gTGCTTTCACTTCTCGCAGGCGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGATCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGt                                                                                                                 <  8:209460/149‑1 (MQ=255)
                              gCTTTCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTgg                                                                                                               >  3:251715/1‑149 (MQ=255)
                               cTTTCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTgg                                                                                                               <  4:251715/148‑1 (MQ=255)
                                tttCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCg                                                                                                             >  5:48572/1‑149 (MQ=255)
                                   cACTTCTCGCAGGCGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTCCCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAg                                                                                                           <  4:292434/148‑1 (MQ=255)
                                   cACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGc                                                                                                          >  5:29744/1‑149 (MQ=255)
                                    aCTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCt                                                                                                         <  2:338255/149‑1 (MQ=255)
                                      ttCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGa                                                                                                       >  4:267170/1‑149 (MQ=255)
                                           gCAGGAGTCCTAGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGTCa                                                                                                     <  3:353441/146‑1 (MQ=255)
                                             aGGAGTCCTCGTATGGTAAGCAACGCCTCCTCATTAGGACGCAAAGGCGTACATGATTTCATCATCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATg                                                                                                 >  4:294666/1‑148 (MQ=255)
                                              ggAGTCCTCGTATGGTAAGCACCGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTCCCAGTGGCGAGCTGACATATg                                                                                                 >  4:225269/1‑147 (MQ=255)
                                              ggAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGaa                                                                                               <  5:334981/149‑1 (MQ=255)
                                                 gTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTc                                                                                            <  5:368775/149‑1 (MQ=255)
                                                    cTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGa                                                                                                       <  2:361348/135‑1 (MQ=255)
                                                    cTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGa                                                                                                       >  1:361348/1‑135 (MQ=255)
                                                     tCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGa                                                                                        >  5:349405/1‑149 (MQ=255)
                                                     tCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGa                                                                                        >  2:8578/1‑149 (MQ=255)
                                                     tCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGa                                                                                        >  7:304089/1‑149 (MQ=255)
                                                      cGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTTGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGAt                                                                                       >  1:236253/1‑149 (MQ=255)
                                                       gTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGa                                                                                        <  8:130726/147‑1 (MQ=255)
                                                       gTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGa                                                                                        <  2:235478/147‑1 (MQ=255)
                                                          tGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGt                                                                                   <  4:253285/149‑1 (MQ=255)
                                                               aGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATGATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTttctt                                                                              <  4:50312/149‑1 (MQ=255)
                                                               aGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTttctt                                                                              <  3:371450/149‑1 (MQ=255)
                                                                gCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTttcttc                                                                             <  6:349405/149‑1 (MQ=255)
                                                                gCAACGCCTCCGCATTAGGACGCAATGGAGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTttcttc                                                                             <  5:275578/149‑1 (MQ=255)
                                                                   aCGCCTCCGCATTAGGACGCAATGGCGTACATTATTTCAACCTCGTTCGCCCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCCAGCTGACATATGAACTCTGGATCGGTATCTTCgac                                                                          >  6:70832/1‑147 (MQ=255)
                                                                    cGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCct                                                                         >  6:356615/1‑149 (MQ=255)
                                                                       cTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCGTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGAGCGGTTTCTTCGCCTCTg                                                                      >  4:320142/1‑149 (MQ=255)
                                                                       cTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTg                                                                      >  1:345070/1‑149 (MQ=255)
                                                                        tCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGc                                                                     >  1:64903/1‑149 (MQ=255)
                                                                         ccGCATTAGGACCCAATGGCGTACATGATTTCAACCTCGATCGCGCTACCGCTATAGTCCTGACGCTCTAAATCATTTATATGGTCGGTATTTTCGCTACCAGTGGCGAGCTCACATAAGAAGTCTGGATCGGTTTCTTCGCCTCTCCg                                                                    >  2:169841/1‑149 (MQ=255)
                                                                           gCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTTACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGtt                                                                  >  7:30061/1‑149 (MQ=255)
                                                                           gCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGATGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGtt                                                                  >  3:172147/1‑149 (MQ=255)
                                                                             aTTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCa                                                                >  7:267870/1‑149 (MQ=255)
                                                                              ttAGGACTCAATGGCGTACATTATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATAGGTTTCTTCGCCTCTGCGTTCAc                                                               <  3:50162/149‑1 (MQ=255)
                                                                                  gACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCaaa                                                           <  8:226463/149‑1 (MQ=255)
                                                                                  gACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCaaa                                                           <  2:52859/149‑1 (MQ=255)
                                                                                  gACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCaaa                                                           <  7:148457/149‑1 (MQ=255)
                                                                                    cGCAATTGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAgt                                                         <  3:237655/149‑1 (MQ=255)
                                                                                    cGCAATGGCGTACTTGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAgt                                                         <  1:47072/149‑1 (MQ=255)
                                                                                       aaTGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGtt                                                      >  7:267102/1‑149 (MQ=255)
                                                                                           gCGTACATGATTTCCTCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCTCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCAcc                                                  <  8:361946/149‑1 (MQ=255)
                                                                                           gCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCAcc                                                  <  3:128613/149‑1 (MQ=255)
                                                                                               aCATGATTTCATCCTCGTTCGCGCTTCCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGc                                                                                                              <  2:39204/85‑1 (MQ=255)
                                                                                               aCATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGc                                                                                                              >  1:39204/1‑85 (MQ=255)
                                                                                               aCATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCctgc                                              >  1:208624/1‑149 (MQ=255)
                                                                                                cATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGtt                                                                  <  6:366037/128‑1 (MQ=255)
                                                                                                cATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGtt                                                                  >  5:366037/1‑128 (MQ=255)
                                                                                                cATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCctgct                                             <  1:313158/148‑1 (MQ=255)
                                                                                                 aTGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGt                                                                   <  5:407536/126‑1 (MQ=255)
                                                                                                 aTGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCctgctg                                            >  5:153549/1‑149 (MQ=255)
                                                                                                  tGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTgg                                           >  1:206180/1‑149 (MQ=255)
                                                                                                    aTTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGc                                                                           >  6:260288/1‑115 (MQ=255)
                                                                                                    aTTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGc                                                                           <  5:260288/115‑1 (MQ=255)
                                                                                                         aTCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGtt                                    >  3:380268/1‑149 (MQ=255)
                                                                                                         aTCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGtt                                    >  3:51902/1‑149 (MQ=255)
                                                                                                            cTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGtttt                                  <  2:345070/148‑1 (MQ=255)
                                                                                                            cTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGtttt                                  <  2:64903/148‑1 (MQ=255)
                                                                                                            cTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTc                                 >  1:339872/1‑149 (MQ=255)
                                                                                                            cTCGTTCGCGCGACCGCTATCGTCCTGACGCGCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATCAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTc                                 <  2:404816/149‑1 (MQ=255)
                                                                                                             tCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTc                                 >  3:382994/1‑148 (MQ=255)
                                                                                                             tCGTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTc                                 >  4:113403/1‑148 (MQ=255)
                                                                                                               gTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGgcgc                                        >  5:51977/1‑139 (MQ=255)
                                                                                                               gTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGgcgc                                        <  6:51977/139‑1 (MQ=255)
                                                                                                               gTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCt                                <  8:304089/147‑1 (MQ=255)
                                                                                                               gTTCGCGCTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCt                                <  6:48572/147‑1 (MQ=255)
                                                                                                                     gcTACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCTAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGAt                        <  6:311858/149‑1 (MQ=255)
                                                                                                                       tACCGCTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATc                       >  8:123106/1‑148 (MQ=255)
                                                                                                                            cTATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGcc                 >  1:11184/1‑149 (MQ=255)
                                                                                                                             tATCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCTCTGTTTTCTATCTTGATCCATGCCt                >  6:371028/1‑149 (MQ=255)
                                                                                                                               tCGTCCTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCt                <  5:356615/147‑1 (MQ=255)
                                                                                                                                   ccTGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCg          >  5:285775/1‑149 (MQ=255)
                                                                                                                                     tGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCg                                                                    >  8:347535/1‑89 (MQ=255)
                                                                                                                                     tGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCg                                                                    <  7:347535/89‑1 (MQ=255)
                                                                                                                                     tGACGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCgg         >  8:381249/1‑148 (MQ=255)
                                                                                                                                       aCGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGc        <  4:382994/147‑1 (MQ=255)
                                                                                                                                       aCGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCAt      <  3:225269/149‑1 (MQ=255)
                                                                                                                                       aCGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCAt      <  6:153549/149‑1 (MQ=255)
                                                                                                                                        cGCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCAtg     <  8:223913/149‑1 (MQ=255)
                                                                                                                                         gCTCTAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCAtgt    <  5:70832/149‑1 (MQ=255)
                                                                                                                                           tctAAATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTgg  >  1:42151/1‑149 (MQ=255)
                                                                                                                                           tctAAATCATTTATATGGTCGGTTATTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGCTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATAGGCATGTgg  >  4:83072/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCGTACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGG  >  NC_000913/755503‑755790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: