Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,303 C→A 92.4% R133S (CGC→AGC)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,3030CA92.4% 228.6 / 10.6 79R133S (CGC→AGC) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (35/38);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

TATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTA  >  NC_000913/756167‑756447
                                                                                                                                        |                                                                                                                                                
tatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCgg                                                                                                                                         >  3:389617/1‑146 (MQ=255)
tatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGgc                                                                                                                                        <  8:142509/147‑1 (MQ=255)
  tgtgTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGgcagc                                                                                                                                     <  6:45706/148‑1 (MQ=255)
  tgtgTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGgcag                                                                                                                                      >  3:254749/1‑147 (MQ=255)
     gtAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCagcagc                                                                                                                                  >  1:230980/1‑148 (MQ=255)
     gtAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCagcagc                                                                                                                                  >  4:324849/1‑148 (MQ=255)
        aaaCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGa                                                                                                                               >  8:235544/1‑148 (MQ=255)
         aaCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTTGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGcagcag                                                                                                                                   <  7:300627/143‑1 (MQ=255)
         aaCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTTGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGcagcag                                                                                                                                   >  8:300627/1‑143 (MQ=255)
         aaCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGAc                                                                                                                              >  1:94689/1‑148 (MQ=255)
         aaCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGAc                                                                                                                              >  1:125773/1‑148 (MQ=255)
          aCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGAcc                                                                                                                             >  1:239767/1‑148 (MQ=255)
            cGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGt                                                                                                                           <  2:94689/148‑1 (MQ=255)
             gggCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTa                                                                                                                          <  6:111101/148‑1 (MQ=255)
             gggCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTa                                                                                                                          <  4:254749/148‑1 (MQ=255)
                ccGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCg                                                                                                                       <  8:293772/148‑1 (MQ=255)
                   gAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTc                                                                                                                    <  3:74407/148‑1 (MQ=255)
                    aaGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCa                                                                                                                   >  5:403364/1‑148 (MQ=255)
                    aaGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCa                                                                                                                   <  7:236387/148‑1 (MQ=255)
                         aTTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCAc                                                                                                              >  2:11359/1‑148 (MQ=255)
                           tCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTg                                                                                                            >  8:357201/1‑148 (MQ=255)
                               gAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGt                                                                                                           <  5:321433/145‑1 (MQ=255)
                               gAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTg                                                                                                         <  3:324849/147‑1 (MQ=255)
                                 aCTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGTCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGcac                                                                                                      <  8:317009/148‑1 (MQ=255)
                                    cGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACg                                                                                                   >  3:268392/1‑148 (MQ=255)
                                    cGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACg                                                                                                   >  4:272709/1‑148 (MQ=255)
                                        cacaTGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCttt                                                                                               <  4:268392/148‑1 (MQ=255)
                                            tGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTAt                                                                                             >  1:359144/1‑146 (MQ=255)
                                             gggCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATcag                                                                                          >  1:396148/1‑148 (MQ=255)
                                             gggCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATcag                                                                                          >  6:356193/1‑148 (MQ=255)
                                               gccggccgTTCTCGCGTCTCGATGATGGGCGTATCTATCCACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATcagca                                                                                        <  8:331074/148‑1 (MQ=255)
                                                   gCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGgcaagca                                                                                                                                               >  7:251656/1‑89 (MQ=255)
                                                   gCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGgcaagca                                                                                                                                               <  8:251656/89‑1 (MQ=255)
                                                   gCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTc                                                                                                                    >  6:386536/1‑116 (MQ=255)
                                                   gCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTc                                                                                                                    <  5:386536/116‑1 (MQ=255)
                                                   gCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAAc                                                                                    >  6:386181/1‑148 (MQ=255)
                                                   gCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAAc                                                                                    >  7:359152/1‑148 (MQ=255)
                                                        tctcGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGa                                                                                >  4:43796/1‑147 (MQ=255)
                                                           cgcgTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGaaaaa                                                                            >  8:215836/1‑148 (MQ=255)
                                                           cgcgTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGaaaaa                                                                            >  7:66280/1‑148 (MQ=255)
                                                            gcgTCTCGGTGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGaaaa                                                                             <  4:79052/146‑1 (MQ=255)
                                                             cgTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAacc                                                                          <  7:395946/148‑1 (MQ=255)
                                                             cgTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAacc                                                                          <  1:11359/148‑1 (MQ=255)
                                                             cgTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAac                                                                           >  7:394950/1‑147 (MQ=255)
                                                              gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAacca                                                                         <  3:43796/148‑1 (MQ=255)
                                                              gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAacca                                                                         <  1:86196/148‑1 (MQ=255)
                                                                ctcGATGATGGGCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAaccacc                                                                       <  4:33897/148‑2 (MQ=255)
                                                                    atgatgGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAaccacaccac                                                                   >  2:328934/1‑148 (MQ=255)
                                                                       atgGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGGCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCAt                                                                <  7:235544/148‑1 (MQ=255)
                                                                               atctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGgcaagc                                                                                                                                                >  6:148165/1‑60 (MQ=255)
                                                                               atctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGgcaagc                                                                                                                                                <  5:148165/60‑1 (MQ=255)
                                                                               atctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATcagc                                                                                         <  1:292493/115‑1 (MQ=255)
                                                                               atctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATcagc                                                                                         >  2:292493/1‑115 (MQ=255)
                                                                                 ctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGc                                                                                                        >  8:313723/1‑98 (MQ=255)
                                                                                 ctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGc                                                                                                        <  7:313723/98‑1 (MQ=255)
                                                                                   atcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTg                                                    >  4:67086/1‑148 (MQ=255)
                                                                                    tcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTg                                                                                                            >  1:36616/1‑91 (MQ=255)
                                                                                    tcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTg                                                                                                            <  2:36616/91‑1 (MQ=255)
                                                                                    tcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACCCCTCCATTTTCTCCGAGTg                                                    <  5:356193/147‑1 (MQ=255)
                                                                                    tcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCCTTTTCTCCgcg                                                      <  2:239767/145‑3 (MQ=255)
                                                                                    tcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGa                                                       <  5:386181/144‑1 (MQ=255)
                                                                                    tcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGGACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTgg                                                   <  2:230980/148‑1 (MQ=255)
                                                                                        cGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTg                                                                                                            >  6:328982/1‑87 (MQ=255)
                                                                                        cGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTg                                                                                                            <  5:328982/87‑1 (MQ=255)
                                                                                            cGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCt                                           <  2:359144/148‑1 (MQ=255)
                                                                                            cGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCt                                           <  2:396148/148‑1 (MQ=255)
                                                                                                 ggCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATc                                      >  1:442776/1‑148 (MQ=255)
                                                                                                     gTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGt                                  <  3:67086/148‑1 (MQ=255)
                                                                                                          tCGAAAAACTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGaaa                              >  7:114864/1‑147 (MQ=255)
                                                                                                             aaaaaCTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTgg                                                   >  3:324539/1‑123 (MQ=255)
                                                                                                             aaaaaCTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTgg                                                   <  4:324539/123‑1 (MQ=255)
                                                                                                             aaaaaCTTCGGCGGCGACCAGGCGGCAAGCACTGCGGCAGAACCTGACCGTACAGGTCACGCACTCATGCACACGCTTTATCAGCAGAACCTCAAAAACCACACCACCATTTTATCCGAGAGGTAAGCGCTGGATCTGGTGAAAAAc                           >  3:37437/1‑147 (MQ=255)
                                                                                                              aaaaCTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCa                         <  3:119308/148‑1 (MQ=255)
                                                                                                               aaaCTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAACCCACACCCCCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAg                        >  2:307381/1‑148 (MQ=255)
                                                                                                               aaaCTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAg                        >  4:41090/1‑148 (MQ=255)
                                                                                                                aaCTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAgg                       <  7:215836/148‑1 (MQ=255)
                                                                                                                  cTTCGGCGGCGAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGAt                     <  6:403364/148‑1 (MQ=255)
                                                                                                                       gcggcgAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGgcgc                <  3:272709/148‑1 (MQ=255)
                                                                                                                         ggcgAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGTTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCgg              <  8:359152/148‑1 (MQ=255)
                                                                                                                           cgAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCggtg            >  8:49084/1‑148 (MQ=255)
                                                                                                                           cgAGCAGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCggtg            <  8:66280/148‑1 (MQ=255)
                                                                                                                                aGGCGGCAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTggg       >  1:99946/1‑148 (MQ=255)
                                                                                                                                 ggcggcAAGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGt      <  7:357201/148‑1 (MQ=255)
                                                                                                                                     gcaagcacTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTa  <  8:394950/148‑1 (MQ=255)
                                                                                                                                        cgcacTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATgc                                              <  8:141519/101‑1 (MQ=255)
                                                                                                                                        cgcacTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATgc                                              >  7:141519/1‑101 (MQ=255)
                                                                                                                                        |                                                                                                                                                
TATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTA  >  NC_000913/756167‑756447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: