Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,224,829 A→G 5.5% L178P (CTG→CCG)  minD ← inhibitor of FtsZ ring polymerization; chromosome‑membrane tethering protein; membrane ATPase of the MinCDEE system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,224,8290AG 5.5% 230.1 / 2.9 72L178P (CTG→CCG) minDinhibitor of FtsZ ring polymerization; chromosome‑membrane tethering protein; membrane ATPase of the MinCDEE system
Reads supporting (aligned to +/- strand):  ref base A (38/30);  new base G (2/2);  total (40/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CGGTTCACCCTGGTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAA  >  NC_000913/1224684‑1224974
                                                                                                                                                 |                                                                                                                                                 
cGGTTCACCCTGGTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAgg                                                                                                                                                 >  6:25770/1‑148 (MQ=255)
   ttCACCCTGGTTAGAGGCGCGCAATACTGATTGGTCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGc                                                                                                                                              >  2:346253/1‑148 (MQ=255)
   ttCACCCTGGTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGc                                                                                                                                              >  2:60998/1‑148 (MQ=255)
   ttCACCCTGGTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGc                                                                                                                                              >  1:173746/1‑148 (MQ=255)
      aCCCTGGTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGTGTTATAGCGCGTTAACAGCAGGTGctct                                                                                                                                           <  6:307677/148‑1 (MQ=255)
           ggTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTaa                                                                                                                                       >  6:267302/1‑147 (MQ=255)
            gTTAGAGGCGCGCAATACTGAGTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCACTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAAt                                                                                                                                      <  3:73693/147‑1 (MQ=255)
             ttAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAg                                                                                                                                    <  1:60998/148‑1 (MQ=255)
              tAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAgg                                                                                                                                   >  8:12476/1‑148 (MQ=255)
                gagGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGct                                                                                                                                 >  1:56899/1‑148 (MQ=255)
                        cAATACTGATTGATCCTCTGGGATCCCGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCa                                                                                                                         >  2:232472/1‑148 (MQ=255)
                        cAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCCGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCGGGTGCTCTTTAATAGGCTCTTCGCCa                                                                                                                         >  2:131112/1‑148 (MQ=255)
                        cAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCGGGTGCTCTTTAATAGGCTCTTCGCCa                                                                                                                         >  2:125208/1‑148 (MQ=255)
                            aCTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAAcagca                                                                                                                                                   >  3:77399/1‑118 (MQ=255)
                            aCTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAAcagca                                                                                                                                                   <  4:77399/118‑1 (MQ=255)
                            aCTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCAtttt                                                                                                                     >  7:249367/1‑148 (MQ=255)
                                            ggATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGAttt                                                                                                     <  2:310271/148‑1 (MQ=255)
                                            ggATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGAtt                                                                                                      <  8:249367/147‑1 (MQ=255)
                                            ggATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCATCCATGCTCAGCAGGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGAttt                                                                                                     <  2:197256/148‑1 (MQ=255)
                                               tCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTa                                                                                                                                        <  4:251103/110‑1 (MQ=255)
                                               tCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTa                                                                                                                                        >  3:251103/1‑110 (MQ=255)
                                                     cgacgaGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAAcagca                                                                                                                                                   >  4:314399/1‑93 (MQ=255)
                                                     cgacgaGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAAcagca                                                                                                                                                   <  3:314399/93‑1 (MQ=255)
                                                      gacgaGTTTGATGCGCAGGATCGCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGa                                                                                           <  5:17373/148‑1 (MQ=255)
                                                          aGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAg                                                                                                                                    <  8:421257/103‑1 (MQ=255)
                                                          aGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAg                                                                                                                                    >  7:421257/1‑103 (MQ=255)
                                                            tttGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCt                                                                                     <  5:357931/148‑1 (MQ=255)
                                                             ttGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGaa                                                                                          >  3:273860/1‑142 (MQ=255)
                                                                     cAGGATCTCCAGCACATGTTCCATGCTCAGCATGTCTCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACgg                                                                            <  1:232472/148‑1 (MQ=255)
                                                                     cAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACgg                                                                            <  5:267302/148‑1 (MQ=255)
                                                                     cAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACgg                                                                            >  1:45933/1‑148 (MQ=255)
                                                                     cAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGAGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACgg                                                                            >  2:403894/1‑148 (MQ=255)
                                                                         aTCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTc                                                                                                                    <  2:276459/104‑1 (MQ=255)
                                                                         aTCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTc                                                                                                                    >  1:276459/1‑104 (MQ=255)
                                                                         aTCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGt                                                                           >  6:174282/1‑145 (MQ=255)
                                                                              cAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTcg                                                                   <  5:174282/148‑1 (MQ=255)
                                                                               aGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTcgc                                                                  >  3:320204/1‑148 (MQ=255)
                                                                                 cacaTCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGt                                                                <  5:25770/148‑1 (MQ=255)
                                                                                    aTCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATAc                                                                              >  2:142339/1‑131 (MQ=255)
                                                                                    aTCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATAc                                                                              <  1:142339/131‑1 (MQ=255)
                                                                                    aTCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACt                                                             <  1:234501/148‑1 (MQ=255)
                                                                                      cTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGAtt                                                                                                      >  6:122234/1‑105 (MQ=255)
                                                                                      cTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGAtt                                                                                                      <  5:122234/105‑1 (MQ=255)
                                                                                      cTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCag                                                                        <  6:256783/135‑1 (MQ=255)
                                                                                      cTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCag                                                                        >  5:256783/1‑135 (MQ=255)
                                                                                      cTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACt                                                             >  3:81015/1‑146 (MQ=255)
                                                                                        tCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTgag                                                          >  7:92712/1‑147 (MQ=255)
                                                                                        tCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTgag                                                          >  1:194875/1‑147 (MQ=255)
                                                                                         ccATGCTCAGCATGTCCCCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTgag                                                          >  8:360410/1‑146 (MQ=255)
                                                                                         ccATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGGGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTgagg                                                         <  8:281703/147‑1 (MQ=255)
                                                                                           aTGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGgaga                                                      <  1:321641/148‑1 (MQ=255)
                                                                                             gCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGa                                                                                           <  8:38494/109‑1 (MQ=255)
                                                                                             gCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGa                                                                                           >  7:38494/1‑109 (MQ=255)
                                                                                                  gCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCgg                                               >  7:376724/1‑148 (MQ=255)
                                                                                                      gTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTtg                                           >  8:397252/1‑148 (MQ=255)
                                                                                                      gTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTtg                                           >  6:124028/1‑148 (MQ=255)
                                                                                                      gTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACGGTGGCGACTTGCGGGTtg                                           >  6:63602/1‑148 (MQ=255)
                                                                                                       tCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGAAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTtgg                                          >  8:436741/1‑148 (MQ=255)
                                                                                                        cACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTtggt                                         >  5:131924/1‑148 (MQ=255)
                                                                                                           ctctGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCGGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTggtggt                                      <  1:131112/148‑1 (MQ=255)
                                                                                                           ctctGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCGGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTggtggt                                      <  1:125208/148‑1 (MQ=255)
                                                                                                              tGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGta                                     <  7:397252/146‑1 (MQ=255)
                                                                                                              tGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGAGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGAAGCCAGAATGCATAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTGCGGGTTGGTGGtaa                                    >  5:23975/1‑147 (MQ=255)
                                                                                                                cTTACGCGGCCTGGGTTATAGAGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGtaa                                    <  1:403894/145‑1 (MQ=255)
                                                                                                                    cgcgGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGc                             <  2:194875/148‑1 (MQ=255)
                                                                                                                            gggTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTg                     >  8:53700/1‑148 (MQ=255)
                                                                                                                            gggTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTg                     >  3:46608/1‑148 (MQ=255)
                                                                                                                             ggTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTg                     >  4:360588/1‑147 (MQ=255)
                                                                                                                               ttATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCt                            <  7:47685/138‑1 (MQ=255)
                                                                                                                               ttATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCt                            >  8:47685/1‑138 (MQ=255)
                                                                                                                                    gcgcGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGGCAGCGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATag             <  5:124028/148‑1 (MQ=255)
                                                                                                                                     cgcgTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATaga            <  5:63602/148‑1 (MQ=255)
                                                                                                                                     cgcgTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATa              >  3:277813/1‑146 (MQ=255)
                                                                                                                                      gcgTTAACAGCAGGTGCGCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATagag           <  1:346253/148‑1 (MQ=255)
                                                                                                                                         ttAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACGTCCGGGTTGGTGGTAATAATGGCGTCGTCTGCAAAATAGAGTGc        >  4:280239/1‑148 (MQ=255)
                                                                                                                                            aCAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTTGTGGTAATAATGGCTTCTTCTGCAAAATAGAGTGCCa      <  4:320204/147‑1 (MQ=255)
                                                                                                                                               gcagGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTaa  <  3:161354/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CGGTTCACCCTGGTTAGAGGCGCGCAATACTGATTGATCCTCTGGGATCACGCCGACGAGTTTGATGCGCAGGATCTCCAGCACATCTTCCATGCTCAGCATGTCACCTCTGCTTACGCGGCCTGGGTTATAGCGCGTTAACAGCAGGTGCTCTTTAATAGGCTCTTCGCCATTTTCTGCGCGGCGTGATTTCGACGCCAGAATGCCTAAAATACGGTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAA  >  NC_000913/1224684‑1224974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: