Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,552,085 G→T 7.3% intergenic (+94/+313) fdnI → / ← yddM formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,552,0850GT 7.3% 188.7 / 3.8 63intergenic (+94/+313)fdnI/yddMformate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (23/35);  new base T (2/3);  total (25/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAA  >  NC_000913/1551940‑1552232
                                                                                                                                                 |                                                                                                                                                   
tCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGa                                                                                                                                                    <  7:338034/147‑1 (MQ=255)
 cGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAAc                                                                                                                                                  >  5:8335/1‑148 (MQ=255)
   tGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGc                                                                                                                                                <  1:258873/148‑1 (MQ=255)
    gAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTGTTGAACTTTAAGCTCAAAATGGCGCTGTAAAAGGCGACATTTTCATATTGTATACAACGTATGATATGTTCATGCCTGATACGGCGAGAACGcc                                                                                                                                               >  2:77428/1‑148 (MQ=25)
         cGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                          >  5:348128/1‑148 (MQ=255)
              aGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                                                                                                     <  8:440168/148‑1 (MQ=255)
                        gAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCt                                                                                                                           <  6:348128/148‑1 (MQ=255)
                         aaaaaaGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCTGATGCGGCGTGATCGCCTTATCCGGCATGAAAAcgcat                                                                                                                          >  1:415731/1‑144 (MQ=255)
                               gagagTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                                                     <  4:359985/131‑1 (MQ=21)
                               gagagTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                                                                     >  3:359985/1‑131 (MQ=21)
                                 gagTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCa                                                                                                                  <  6:8412/148‑1 (MQ=255)
                                 gagTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCa                                                                                                                  <  8:296733/148‑1 (MQ=255)
                                   gTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCTGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCaa                                                                                                                 >  1:354923/1‑147 (MQ=255)
                                   gTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAAt                                                                                                                <  3:150230/148‑1 (MQ=255)
                                      aagaagGGATTTAATCTCTTTTGAACTTTAAGCTGAAAATGGCGATGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCTGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAg                                                                                                              <  3:262861/147‑1 (MQ=255)
                                          agGGATATAATCTCTTTTGAACTTTAAGCTGAGAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGc                                                                                                         <  6:373603/148‑1 (MQ=255)
                                          agGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCTCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGc                                                                                                         <  8:138396/148‑1 (MQ=255)
                                                ataATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGaa                                                                                                   >  6:335294/1‑148 (MQ=255)
                                                  aaTCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATcc                                                                                                                   <  2:345351/130‑1 (MQ=255)
                                                  aaTCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATcc                                                                                                                   >  1:345351/1‑130 (MQ=255)
                                                  aaTCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACg                                                                                                 >  3:355639/1‑148 (MQ=255)
                                                  aaTCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACg                                                                                                 >  7:44599/1‑148 (MQ=255)
                                                   aTCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGaa                                                                                                   <  8:426842/145‑1 (MQ=255)
                                                   aTCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGaa                                                                                                   >  7:426842/1‑145 (MQ=255)
                                                      tctTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgtg                                                                                               >  4:207201/1‑146 (MQ=255)
                                                      tattttGATTTTTAAGTTGAAAATGTATCTGTAATATGCGCAATTTTAATATTGTATAAAACTTTTGATTTGTTCATGCCGGATTAGGCGTTAACGCCTTATCCGGATTGAAAACCTTTCAAATCCAATAGATTGCAGTGAACgtgt                                                                                              <  8:235423/145‑1 (MQ=2)
                                                        ttttGAATTTTTAGCTGAAAATTGCTCTGTAATATTCTACATTTTAATATTGTATTCTAATTTGTCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgtgt                                                                                              <  2:281616/145‑1 (MQ=14)
                                                        ttttGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgtgt                                                                                              <  8:44599/145‑1 (MQ=255)
                                                          ttGAACTTTATGCTGAACATGGCGCTGTAAATGGCTCGATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATGCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGc                                                                                          <  1:262133/147‑1 (MQ=14)
                                                             aaCTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTa                                                                                             >  2:14415/1‑141 (MQ=255)
                                                             aaCTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTa                                                                                             <  1:14415/141‑1 (MQ=255)
                                                                 ttAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAg                                                                                  >  2:419962/1‑148 (MQ=39)
                                                                 ttAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAg                                                                                  >  2:413844/1‑148 (MQ=39)
                                                                    aGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGt                                                                               <  5:98729/148‑1 (MQ=37)
                                                                      cTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAg                                                                             <  3:207201/148‑1 (MQ=35)
                                                                                     tAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAt                                                                <  5:335294/146‑1 (MQ=32)
                                                                                     tAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGt                                                              >  3:223698/1‑148 (MQ=32)
                                                                                     tAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGt                                                              <  7:240713/148‑1 (MQ=32)
                                                                                     tAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGt                                                              <  5:16996/148‑1 (MQ=32)
                                                                                      aaaaGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGACAACCCTTAAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATAAGGCAATGtt                                                             >  3:141971/1‑148 (MQ=12)
                                                                                                           gTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCaa                                         <  6:137111/147‑1 (MQ=32)
                                                                                                           gTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGAATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCaa                                         <  4:4961/147‑1 (MQ=17)
                                                                                                              gACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATg                                      <  1:419962/147‑1 (MQ=34)
                                                                                                              gACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATg                                      <  1:413844/147‑1 (MQ=34)
                                                                                                                  aCGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCt                                 >  3:125100/1‑148 (MQ=34)
                                                                                                                  aCGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCt                                 >  1:175943/1‑148 (MQ=34)
                                                                                                                    gTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTg                                >  5:93009/1‑147 (MQ=35)
                                                                                                                    gTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTg                                >  3:101487/1‑147 (MQ=35)
                                                                                                                     tAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCGTATCCGGCATGAAACCCCTTCAAATCCAATAGAGTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCAGCAGTTTCAAATGGCGCTGt                               >  7:98094/1‑147 (MQ=255)
                                                                                                                       ggCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTaaa                            <  2:354923/148‑1 (MQ=38)
                                                                                                                            tGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAAGGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGc                        <  5:398155/147‑1 (MQ=255)
                                                                                                                              ttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTc                     <  4:223698/148‑1 (MQ=255)
                                                                                                                               tCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCa                    >  7:300076/1‑148 (MQ=255)
                                                                                                                                 aTGCCGGATGCGGCGTGAAGGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGCACGTGTAGGCCTGATAAGCGGAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCAtt                  <  2:364691/148‑1 (MQ=255)
                                                                                                                                 aTGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTTTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCAt                   <  4:355639/147‑1 (MQ=255)
                                                                                                                                  tGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACCTGTAGGCCTGATAAGCGTAGCGCATAAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCAttt                 >  8:79296/1‑148 (MQ=14)
                                                                                                                                   gCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCAttt                 >  6:37340/1‑147 (MQ=255)
                                                                                                                                      ggATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCat             <  6:93009/148‑1 (MQ=255)
                                                                                                                                       gATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCata            <  2:175943/148‑1 (MQ=255)
                                                                                                                                         tGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAACTCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATAtt          <  8:98094/148‑1 (MQ=255)
                                                                                                                                           cggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGt        <  4:125100/148‑1 (MQ=255)
                                                                                                                                            ggcgTTATCGCCTTATCCGGCATGAAAACCCTTCAAATAAAATAGATTGCAGTGAAAGTGTATTCCTGATAAGCGTAGCGCATCAGTGAATGTTTCTTTTGTCATCATTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTa       <  1:77428/148‑1 (MQ=17)
                                                                                                                                              cgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGa     <  2:48279/148‑1 (MQ=255)
                                                                                                                                                 gAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACaa  >  1:186383/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAA  >  NC_000913/1551940‑1552232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: