| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,729,944 | T→C | 6.7% | L286L (CTT→CTC) | lhr → | putative ATP‑dependent helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,729,944 | 0 | T | C | 6.7% | 184.2 / 3.6 | 60 | L286L (CTT→CTC) | lhr | putative ATP‑dependent helicase |
| Reads supporting (aligned to +/- strand): ref base T (25/31); new base C (2/2); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01 | |||||||||||
CCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGGCCCATTTCGAGTC > NC_000913/1729798‑1730090 | ccgcccgcAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTtg < 3:409035/148‑1 (MQ=255) cgcAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAAGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGaa < 1:92928/148‑1 (MQ=255) aTGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATTTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAgtgt < 4:127722/148‑1 (MQ=255) cgcCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTgc > 2:379780/1‑148 (MQ=255) ccGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATc > 5:388763/1‑148 (MQ=255) cAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCt > 3:288400/1‑148 (MQ=255) aCGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTCGATGAAGTGTTGCGCCATCGCTCGAc > 8:725/1‑147 (MQ=255) cGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTgc < 1:186980/133‑1 (MQ=255) cGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTgc > 2:186980/1‑133 (MQ=255) gAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCAt > 5:219887/1‑148 (MQ=255) ttGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGt < 8:274884/148‑1 (MQ=255) cGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTTTCtt > 3:264863/1‑147 (MQ=255) gTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTa < 1:379780/148‑1 (MQ=255) tACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTtg > 5:83766/1‑111 (MQ=255) tACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTtg < 6:83766/111‑1 (MQ=255) ccGGTCGACAATATGAATGATGGCTCATCGTGCGCCAGCTGCACCGGCGCAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGCTGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTa < 8:421262/148‑1 (MQ=255) tCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTc < 3:278840/148‑1 (MQ=255) tCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTc < 3:284368/148‑1 (MQ=255) gCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTcg < 6:388763/147‑1 (MQ=255) ccAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTcgcg > 8:125964/1‑148 (MQ=255) atGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGTAGTGTTGCGCCATCGCTCGACCATTGTCTTTACGAATTCGCGGGGGc > 4:268017/1‑148 (MQ=255) tGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCt < 6:219887/148‑1 (MQ=255) tgatgTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTCGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCg > 5:155144/1‑148 (MQ=255) tctcATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGaaaa < 8:248225/148‑1 (MQ=255) ctcATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGaaaaa > 8:1652/1‑148 (MQ=255) aTCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGTGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGaaaaa < 8:191307/145‑1 (MQ=255) tCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTg > 7:82115/1‑147 (MQ=255) tCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTg < 8:82115/147‑1 (MQ=255) tCGGGCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGa < 6:190864/148‑1 (MQ=255) cGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGAc > 8:283879/1‑148 (MQ=255) cGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCa > 5:73001/1‑148 (MQ=255) cAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGa > 8:216153/1‑148 (MQ=255) aGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGAt < 6:73001/148‑1 (MQ=255) ggCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCGGGCGGAAAAACTGACGGCACGATTaa < 5:88298/148‑1 (MQ=255) aCCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAAt > 1:66959/1‑147 (MQ=255) aCCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATg < 3:326112/148‑1 (MQ=255) cGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCtt > 1:163875/1‑148 (MQ=255) gAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTCGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCttt < 7:725/148‑1 (MQ=255) cAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTAcgcc > 4:43975/1‑148 (MQ=255) cAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTAcgcc < 4:288400/148‑1 (MQ=255) aGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTCGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTAcgccg < 6:155144/148‑1 (MQ=255) gccggccgGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGAGCATTGTCTTTACTAGTTGGCGGGGGCTGGCGGCGAGACTGACGGGAGGATTATATGAGCTTTGCGCCGCACGCt > 4:332590/1‑148 (MQ=255) aaGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTACGCCGCACGCTTACAGCGTTc < 7:216153/148‑1 (MQ=255) ggCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTACGCCGCACGCTTACAGCGTTccc < 2:163875/148‑1 (MQ=255) cTCCATCTGGACATATATTTAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCTGGGTCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTACGCCGCACGCTTACAGCGTTCCCCg < 3:11044/148‑1 (MQ=255) cATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTa < 6:412015/71‑1 (MQ=255) cATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTa > 5:412015/1‑71 (MQ=255) tCTGGACATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATAGCTCTACCATTCTCATTACTAATTAGCCGGGGCTGGCGGAAAAACAGACGGCAAGATTAAATGAGCTTTACGCCGCACGCTTACAGCGTTCCACGTCTAt > 4:215825/1‑148 (MQ=255) ccATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGc > 6:280074/1‑80 (MQ=255) ccATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGc < 5:280074/80‑1 (MQ=255) cATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGt < 7:283879/148‑1 (MQ=255) atTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATg > 4:352595/1‑148 (MQ=255) ttGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAg > 3:178616/1‑101 (MQ=255) ttGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAg < 4:178616/101‑1 (MQ=255) ttGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATg < 3:268017/147‑1 (MQ=255) gggTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTcgcg < 5:194551/57‑1 (MQ=255) gggTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTcgcg > 6:194551/1‑57 (MQ=255) ggTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGGCCCAtt < 2:401393/148‑1 (MQ=255) gTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGGCCCAttt < 3:352595/148‑1 (MQ=255) aTCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGGCCCATTTc > 7:309538/1‑147 (MQ=255) tCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGGCCCATTTCg < 7:125964/147‑1 (MQ=255) ccTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCttt > 1:390990/1‑92 (MQ=255) ccTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCttt < 2:390990/92‑1 (MQ=255) ttGATGAAGTGTTGCGCCATCTCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGGCCCATTTCGAGTc > 1:437668/1‑148 (MQ=255) | CCGCCCGCAATGCGCCATCCGCAGATACGAATTGTCGTACCGGTCGCCAATATGGATGATGTCTCATCGGTCGCCAGCGGCACCGGCGAAGACAGCCATGCCGGCCGGGAAGGCTCCATCTGGCCATATATTGAAACGGGTATCCTTGATGAAGTGTTGCGCCATCGCTCGACCATTGTCTTTACTAATTCGCGGGGGCTGGCGGAAAAACTGACGGCACGATTAAATGAGCTTTACGCCGCACGCTTACAGCGTTCCCCGTCTATCGCCGTTGATGCGGCCCATTTCGAGTC > NC_000913/1729798‑1730090 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |