Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,750,351 A→G 5.2% N221N (AAT→AAC ydhX ← putative 4Fe‑4S ferridoxin‑type protein; FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,750,3510AG 5.2% 241.8 / 2.8 76N221N (AAT→AACydhXputative 4Fe‑4S ferridoxin‑type protein; FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex
Reads supporting (aligned to +/- strand):  ref base A (32/40);  new base G (2/2);  total (34/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

CAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGT  >  NC_000913/1750211‑1750495
                                                                                                                                            |                                                                                                                                                
cAGCCCCAGCACCAACCACATCGCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCt                                                                                                                                           <  1:74606/148‑1 (MQ=255)
cAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCTGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTcc                                                                                                                                            <  3:13236/147‑1 (MQ=255)
  gCCCCAGCACCAACCACATCCCAACCAGCAATACTCCCGCAATGATCAACCACACCGGCCAAAATTGGGGAGTGAACTGTGGCACATAGTTCGATAACTGGCTCTGAAACTGTTCAGCATGTTGCGACTGTTTCATACATTTTCCttt                                                                                                                                         >  4:112642/1‑148 (MQ=255)
   ccccAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCtttt                                                                                                                                        >  3:355433/1‑148 (MQ=255)
      cAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTaa                                                                                                                                     >  4:304380/1‑148 (MQ=255)
      cAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTaa                                                                                                                                     >  2:105848/1‑148 (MQ=255)
           ccaaccaCATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCaaa                                                                                                                                >  7:266375/1‑148 (MQ=255)
            caaccaCATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAAt                                                                                                                               >  3:241762/1‑148 (MQ=255)
              accaCATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGt                                                                                                                             <  8:266375/148‑1 (MQ=255)
                cacaTCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTg                                                                                                                           <  3:308506/148‑1 (MQ=255)
                cacaTCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATAAATTTTCCTTTTTAATCAAATGtt                                                                                                                            >  7:113551/1‑147 (MQ=255)
                 acaTCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGa                                                                                                                          <  4:412427/148‑1 (MQ=255)
                      ccAACCAGCAATACTCCGTCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGaa                                                                                                                     <  2:251663/148‑1 (MQ=255)
                        aaCCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAAcc                                                                                                                   >  4:211049/1‑148 (MQ=255)
                         aCCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGTGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCg                                                                                                                  <  6:412420/148‑1 (MQ=255)
                         aCCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCg                                                                                                                  <  3:285301/148‑1 (MQ=255)
                         aCCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCg                                                                                                                  >  5:250733/1‑148 (MQ=255)
                               aaTACTCCGGCAATGATCATCCTGACCGGACAAAATTCGGGAGTGATCTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGTTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATa                                                                                                            <  2:4825/148‑1 (MQ=255)
                               aaTACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGAtc                                                                                                            <  1:105848/148‑2 (MQ=255)
                                      cGGCAATTATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATg                                                                                                      <  1:150378/147‑1 (MQ=255)
                                         cAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGt                                                                                                  >  8:288234/1‑148 (MQ=255)
                                            tGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTc                                                                                               <  4:92666/148‑1 (MQ=255)
                                             gATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTcc                                                                                              >  7:161337/1‑148 (MQ=255)
                                              aTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTccc                                                                                             >  8:64269/1‑148 (MQ=255)
                                                     acacGGGACAAAATTGGGGAGTGAACTGTGGCACATAGTTCGCTAACGGGCTCTTAAACTGTACAGCATGTTGCGACGGGTTCATACATTTTCCTTATTTATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCa                                                                                      <  6:246409/148‑1 (MQ=255)
                                                     acacCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCa                                                                                      <  3:211049/148‑1 (MQ=255)
                                                       acCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAgg                                                                                    <  4:355433/148‑1 (MQ=255)
                                                                 aTTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGatat                                                                          <  7:64269/148‑1 (MQ=255)
                                                                    cGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTg                                                                        <  6:250733/147‑1 (MQ=255)
                                                                     gggAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGa                                                                       <  1:335090/147‑1 (MQ=255)
                                                                      ggAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGa                                                                       >  6:175274/1‑146 (MQ=255)
                                                                          tGAACTGTGGCACATAGTTCGCTATCTGGCTCTGATACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGta                                                                  <  4:199126/147‑1 (MQ=255)
                                                                           gAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGtatt                                                                >  2:211065/1‑148 (MQ=255)
                                                                             aCTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGtattta                                                              >  1:394980/1‑148 (MQ=255)
                                                                                gtgGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAAt                                                                                                                               <  4:317000/80‑1 (MQ=255)
                                                                                gtgGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAAt                                                                                                                               >  3:317000/1‑80 (MQ=255)
                                                                                   gCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTtgc                                                        <  7:64580/148‑1 (MQ=255)
                                                                                    cacaTAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTtgct                                                       >  2:37857/1‑148 (MQ=255)
                                                                                     acaTAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTtgctg                                                      <  2:394980/148‑1 (MQ=255)
                                                                                      caTAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTCGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTtgctg                                                      >  7:192989/1‑147 (MQ=255)
                                                                                          gTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGa                                                                       >  5:253345/1‑126 (MQ=255)
                                                                                          gTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGa                                                                       <  6:253345/126‑1 (MQ=255)
                                                                                          gTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAAcc                                                 <  3:304380/148‑1 (MQ=255)
                                                                                            tCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAAcc                                                 <  1:109816/146‑1 (MQ=255)
                                                                                            tCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAAcc                                                 >  2:109816/1‑146 (MQ=255)
                                                                                                    tGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAAcc                                                 <  6:214706/138‑1 (MQ=255)
                                                                                                    tGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAAcc                                                 >  5:214706/1‑138 (MQ=255)
                                                                                                      gCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAAttt                                     >  7:29346/1‑148 (MQ=255)
                                                                                                      gCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAAttt                                     <  1:211065/148‑1 (MQ=255)
                                                                                                       ctctGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTc                                    <  4:241762/148‑1 (MQ=255)
                                                                                                        tctGAAACTGTTCAGCATGTTGCGACGGGTGCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCt                                   <  5:180931/148‑1 (MQ=255)
                                                                                                          tGAAACTGTTCAGCATGTTCCGACGGGTTCATACAGTTTCCTTTTTATTCAAATGTTGAACGAACAGGCTATTCAGAGGAGGTTTCCCGGCACAAGGTATCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTcg                                   <  7:306444/146‑2 (MQ=255)
                                                                                                           gAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCa                                                                                      <  6:311036/94‑1 (MQ=255)
                                                                                                           gAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCa                                                                                      >  5:311036/1‑94 (MQ=255)
                                                                                                            aaaCTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTgg                                 <  5:175274/146‑1 (MQ=255)
                                                                                                             aaCTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCAGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGc                               >  7:202621/1‑147 (MQ=255)
                                                                                                              aCTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTa                             >  8:334962/1‑148 (MQ=255)
                                                                                                                 gTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCt                          >  3:140611/1‑148 (MQ=255)
                                                                                                                    cAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTc                        <  8:161337/147‑1 (MQ=255)
                                                                                                                     aGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTcg                       <  8:113551/147‑1 (MQ=255)
                                                                                                                        aTGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCa                                                                                      <  4:269276/81‑1 (MQ=255)
                                                                                                                        aTGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCa                                                                                      >  3:269276/1‑81 (MQ=255)
                                                                                                                            tGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCaaa               <  8:192989/148‑1 (MQ=255)
                                                                                                                             gCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCa                 >  6:320392/1‑145 (MQ=255)
                                                                                                                                      tCATACGTTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCa          <  8:76842/143‑1 (MQ=255)
                                                                                                                                      tCATACGTTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCa          >  7:76842/1‑143 (MQ=255)
                                                                                                                                      tCATACGTTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCa          >  7:69908/1‑143 (MQ=255)
                                                                                                                                      tCATACGTTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCa          <  8:69908/143‑1 (MQ=255)
                                                                                                                                       cATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCg     <  7:334962/147‑1 (MQ=255)
                                                                                                                                         tACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCCCCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCaa                                                   <  8:430718/99‑1 (MQ=255)
                                                                                                                                         tACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCCCCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCaa                                                   >  7:430718/1‑99 (MQ=255)
                                                                                                                                         tACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCa          >  6:61277/1‑140 (MQ=255)
                                                                                                                                         tACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCa          <  5:61277/140‑1 (MQ=255)
                                                                                                                                         tACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCgtgt  >  2:58748/1‑148 (MQ=255)
                                                                                                                                          aCATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCa                                                    <  8:278322/97‑1 (MQ=255)
                                                                                                                                          aCATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCa                                                    >  7:278322/1‑97 (MQ=255)
                                                                                                                                            |                                                                                                                                                
CAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGT  >  NC_000913/1750211‑1750495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: