Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,085,969 C→G 5.6% G365A (GGT→GCT)  plaP ← putrescine importer, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,9690CG 5.6% 249.1 / 5.7 72G365A (GGT→GCT) plaPputrescine importer, low affinity
Reads supporting (aligned to +/- strand):  ref base C (32/36);  new base G (2/2);  total (34/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

ACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCGAAGAAGCTTTTCGGGAATACGCCGTCACG  >  NC_000913/2085829‑2086114
                                                                                                                                            |                                                                                                                                                 
acaGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGt                                                                                                                                             >  5:178715/1‑147 (MQ=255)
acaGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGt                                                                                                                                             >  5:172323/1‑147 (MQ=255)
 caGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCCCGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGtt                                                                                                                                            >  7:21392/1‑147 (MQ=255)
   gcgcACCAACGGTCAGCGCACAACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCCTCTTGATACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAAt                                                                                                                                         >  2:61355/1‑148 (MQ=255)
     gcACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCa                                                                                                                                       <  6:178715/148‑1 (MQ=255)
     gcACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCATTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCa                                                                                                                                       <  6:172323/148‑1 (MQ=255)
             ggTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGtt                                                                                                                               >  4:129561/1‑148 (MQ=255)
              gTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTg                                                                                                                              >  2:405079/1‑148 (MQ=255)
                    gcACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTa                                                                                                                        <  7:379848/148‑1 (MQ=255)
                            caTCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTc                                                                                                                 >  1:113875/1‑147 (MQ=255)
                                ggCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGGCGAAGt                                                                                                            <  1:61355/148‑1 (MQ=255)
                                  cAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGTCCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCGGTTGCCATTACCAGGTCGAAGTTg                                                                                                          <  4:113495/148‑1 (MQ=255)
                                  cAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCACACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGtt                                                                                                           >  2:40305/1‑147 (MQ=255)
                                              cTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGCCCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGGTGCCATTACCAGGTCGAAGTTGATTGCcagcag                                                                                               <  8:90009/147‑1 (MQ=255)
                                                     tGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATcg                                                                                       <  6:262418/148‑1 (MQ=255)
                                                      gATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCAGCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGc                                                                                            >  3:79438/1‑142 (MQ=255)
                                                      gATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCAGCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGc                                                                                            <  4:79436/142‑1 (MQ=255)
                                                      gATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCAGCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGc                                                                                            <  4:79438/142‑1 (MQ=255)
                                                      gATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCAGCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGc                                                                                            >  3:79436/1‑142 (MQ=255)
                                                      gATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCAtt                                                                                                                         <  3:260220/113‑1 (MQ=255)
                                                      gATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCAtt                                                                                                                         >  1:245914/1‑113 (MQ=255)
                                                      gATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCAtt                                                                                                                         >  4:260220/1‑113 (MQ=255)
                                                      gATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCAtt                                                                                                                         <  2:245914/113‑1 (MQ=255)
                                                         cTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCgcgc                                                                                    >  5:265645/1‑147 (MQ=255)
                                                          tttCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGcc                                                                                   >  8:148569/1‑147 (MQ=255)
                                                              aGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAAcc                                                                               <  1:419713/147‑1 (MQ=255)
                                                                      gTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGgatgat                                                                       <  1:441468/147‑1 (MQ=255)
                                                                      gTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGgatgat                                                                       <  6:404145/147‑1 (MQ=255)
                                                                       ttACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAgg                                                                                              >  6:160961/1‑123 (MQ=255)
                                                                       ttACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAgg                                                                                              <  5:160961/123‑1 (MQ=255)
                                                                       ttACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAAcc                                                                               <  5:370861/138‑1 (MQ=255)
                                                                       ttACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAAcc                                                                               >  6:370861/1‑138 (MQ=255)
                                                                       ttACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGt                                                                     >  8:232493/1‑148 (MQ=255)
                                                                         aCGCTTCTCCCGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGAAGGGAAATCGCGCCACCCAGGATGATGtt                                                                    >  8:178887/1‑147 (MQ=255)
                                                                         aCGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGtt                                                                    <  1:98830/147‑1 (MQ=255)
                                                                          cGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCa                                                                  <  3:129561/148‑1 (MQ=255)
                                                                           gCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCcagcag                                                                                               <  7:396119/118‑1 (MQ=255)
                                                                           gCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCcagcag                                                                                               >  8:396119/1‑118 (MQ=255)
                                                                                 cACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTTCCAGGTCGAAGTTg                                                                                                          >  5:311091/1‑101 (MQ=255)
                                                                                 cACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTg                                                                                                          <  8:29790/101‑1 (MQ=255)
                                                                                 cACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTg                                                                                                          <  6:311091/101‑1 (MQ=255)
                                                                                 cACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTg                                                                                                          >  7:29790/1‑101 (MQ=255)
                                                                                   cGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAg                                                         >  5:97150/1‑148 (MQ=255)
                                                                                   cGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAg                                                         >  5:167893/1‑148 (MQ=255)
                                                                                     gATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTa                                                       <  1:40305/148‑1 (MQ=255)
                                                                                       tCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTAGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACg                                                     >  8:387754/1‑148 (MQ=255)
                                                                                              cTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTc                                              <  7:232493/148‑1 (MQ=255)
                                                                                               tGCGAGATGACAGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCg                                             >  3:372403/1‑148 (MQ=255)
                                                                                                gCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCg                                                                                                                                 >  2:232439/1‑63 (MQ=255)
                                                                                                gCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCg                                                                                                                                 <  1:232439/63‑1 (MQ=255)
                                                                                                gCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCgg                                            >  1:240198/1‑148 (MQ=255)
                                                                                                   agaTGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGgtg                                         <  6:265645/148‑1 (MQ=255)
                                                                                                    gaTGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGgtgt                                        <  2:240198/148‑1 (MQ=255)
                                                                                                     aTGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTa                                       <  7:148569/148‑1 (MQ=255)
                                                                                                         ccGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATa                                   <  8:21392/148‑1 (MQ=255)
                                                                                                           gACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCAATTTCGGGTGTACATAAc                                 >  8:144504/1‑148 (MQ=255)
                                                                                                            aCAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCggg                                           >  3:200696/1‑137 (MQ=255)
                                                                                                            aCAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCggg                                           <  4:200696/137‑1 (MQ=255)
                                                                                                              aGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCga                              <  6:167893/148‑1 (MQ=255)
                                                                                                               gcgtcaCGAAAGGGAACGCCACCAGCCGACCAAAGTTTATCAGCGGAGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCACGGCAATCGCGCCAACCTGGATGATGTTCATCGCCGGCGTACGCCATTTCGGGTGTACATAACCgaa                             <  6:311637/143‑1 (MQ=255)
                                                                                                                   aaCGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAg                                                         >  4:209708/1‑116 (MQ=255)
                                                                                                                   aaCGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAg                                                         <  3:209708/116‑1 (MQ=255)
                                                                                                                       aaaGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCACCCAGGATGATGTTCATCGCCGGAGTACGCCATTTCggg                                           >  5:231402/1‑126 (MQ=255)
                                                                                                                       aaaGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCggg                                           <  6:231402/126‑1 (MQ=255)
                                                                                                                       aaaGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCGAAGAAGCttt                     >  7:152884/1‑148 (MQ=255)
                                                                                                                       aaaGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCGAAGAAGCttt                     >  8:295319/1‑148 (MQ=255)
                                                                                                                        aaGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCGAAGAAGCtttt                    <  1:405079/148‑1 (MQ=255)
                                                                                                                        aaGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCGAAGAAGCtttt                    <  6:97150/148‑1 (MQ=255)
                                                                                                                             aaCGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCGAAGAAGCTTTTCggg                <  7:178887/147‑1 (MQ=255)
                                                                                                                             aaCGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCGAAGAAGCTTTTCggg                <  8:152884/147‑1 (MQ=255)
                                                                                                                                      aGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCGAAGAAGCTTTTCGGGAATACGCCGt      >  5:102415/1‑148 (MQ=255)
                                                                                                                                          cACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCGAAGAAGCTTTTCGGGAATACGCCGTCACg  <  4:413694/148‑1 (MQ=255)
                                                                                                                                            ccAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGATTACGCCATTTCgg                                            <  4:273889/104‑1 (MQ=255)
                                                                                                                                            ccAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCgg                                            >  3:273889/1‑104 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
ACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGCACCAAAGTTAATCAGCGCCGTTGCCATTACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTTCATCGCCGGAGTACGCCATTTCGGGTGTACATAACCGAAGAAGCTTTTCGGGAATACGCCGTCACG  >  NC_000913/2085829‑2086114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: