Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,411,551	G→A	5.0%	A180V (GCG→GTG)	yfbT ←	sugar phosphatas

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,411,551	0	G	A	5.0%	264.8 / 2.4	79	A180V (GCG→GTG)	yfbT	sugar phosphatas
Reads supporting (aligned to +/- strand):  ref base G (30/45);  new base A (2/2);  total (32/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01

CCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCT  >  NC_000913/2411404‑2411695                                                                                                                                                    |                                                                                                                                                 cccGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGAGTATCTGCCGGAGCGTTAACCg                                                                                                                                                  >  3:302846/1‑148 (MQ=255)   cgacgaGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCa                                                                                                                                                <  7:196364/148‑1 (MQ=255)   cgacgaGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCa                                                                                                                                                <  5:431585/148‑1 (MQ=255)    gacgaGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCaa                                                                                                                                               >  5:298204/1‑148 (MQ=255)      cgaGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatg                                                                                                                                             <  4:143211/148‑1 (MQ=255)      cgaGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatg                                                                                                                                             <  2:422757/148‑1 (MQ=255)       gagaCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatga                                                                                                                                            <  4:49112/148‑1 (MQ=255)         gaCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatgaca                                                                                                                                          <  8:212745/148‑1 (MQ=255)            ggggCTAAATCATGATCAGGTGGGTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatgacatga                                                                                                                                       <  5:77326/148‑1 (MQ=255)              ggCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAAtgacatgaca                                                                                                                                     <  4:143936/148‑1 (MQ=255)                cTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAAc                                                                                                                                   >  1:283153/1‑148 (MQ=255)                  aaaTCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAAccc                                                                                                                                 >  7:382456/1‑148 (MQ=255)                      cATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGc                                                                                                                                                 <  2:439228/128‑1 (MQ=255)                      cATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGc                                                                                                                                                 >  1:439228/1‑128 (MQ=255)                        tGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCcgccgc                                                                                                                            >  1:93069/1‑147 (MQ=255)                        tGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCgccgcc                                                                                                                           <  6:210646/148‑1 (MQ=255)                        tGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCgccgcc                                                                                                                           <  7:295884/148‑1 (MQ=255)                          aTCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCgccag                                                                                                                         <  2:103117/148‑1 (MQ=255)                          aTCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCgcca                                                                                                                          <  2:239253/147‑1 (MQ=255)                           tCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCgccagg                                                                                                                        <  1:38209/148‑1 (MQ=255)                           tCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCgccagg                                                                                                                        <  7:227777/148‑1 (MQ=255)                             aGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCgccaggcc                                                                                                                      <  8:382456/148‑1 (MQ=255)                              ggTTTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTGCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCgccaggcca                                                                                                                     <  6:136241/148‑1 (MQ=255)                                  tttCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGaa                                                                                                                  <  7:251875/147‑1 (MQ=255)                                   ttCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCACAATGACATGACAACCCGCCGCCAGGCCAGAAAg                                                                                                                >  5:408151/1‑148 (MQ=255)                                   ttCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCACAATGACATGACAACCCGCCGCCAGGCCAGAAAg                                                                                                                >  5:163295/1‑148 (MQ=255)                                    tCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAg                                                                                                                <  4:232302/147‑1 (MQ=255)                                     cACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGc                                                                                                               <  1:428552/147‑1 (MQ=255)                                     cACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGc                                                                                                               <  2:283153/147‑1 (MQ=255)                                      aCTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCa                                                                                                              <  2:385246/147‑1 (MQ=255)                                        tGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGc                                                                                                           >  5:371082/1‑148 (MQ=255)                                        tGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGc                                                                                                           >  3:222699/1‑148 (MQ=255)                                         gAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGc                                                                                                           >  7:371811/1‑147 (MQ=255)                                           ataataACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAg                                                                                                        <  6:371082/148‑1 (MQ=255)                                               taaCATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCggg                                                                                                    >  7:266202/1‑148 (MQ=255)                                                      gCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATc                                                                                              <  4:352888/147‑1 (MQ=255)                                                         aTTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCACAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTcc                                                                                          <  6:408151/148‑1 (MQ=255)                                                         aTTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCACAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTcc                                                                                          <  6:163295/148‑1 (MQ=255)                                                             ggCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTccacc                                                                                       <  6:372425/147‑1 (MQ=255)                                                              gCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTccaccac                                                                                     <  8:230702/148‑1 (MQ=255)                                                              gCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGAGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACACGCCGCCAGGCCAGAAAGCACGCCAGAGGGAGCATCTTccaccac                                                                                     <  4:302846/148‑1 (MQ=255)                                                               cTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTccaccacc                                                                                    <  8:371811/148‑1 (MQ=255)                                                                         acaGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCTGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCt                                                                          <  2:24107/148‑1 (MQ=255)                                                                          caGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTg                                                                         >  5:154921/1‑148 (MQ=255)                                                                          caGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTg                                                                         >  1:107554/1‑148 (MQ=255)                                                                              aaTTTGCTCCAGACTGTGGATGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAATCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTgcgg                                                                      <  3:134097/147‑1 (MQ=255)                                                                                      ccAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGccc                                                             >  3:354595/1‑148 (MQ=255)                                                                                        aGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGaa                                                                                                                  <  1:182574/93‑1 (MQ=255)                                                                                        aGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGaa                                                                                                                  >  2:182574/1‑93 (MQ=255)                                                                                        aGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCca                                                           <  5:149430/148‑1 (MQ=255)                                                                                        aGACAGTGGAGCACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCAAGGCCAGAACGCACGCCACCGGGAGCATCTTccacc                                                                                       >  5:241772/1‑120 (MQ=255)                                                                                         gACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCcag                                                          <  1:87792/148‑1 (MQ=255)                                                                                         gACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTGAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCcag                                                          >  6:275554/1‑148 (MQ=255)                                                                                          aCTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCcagc                                                         <  7:20148/148‑1 (MQ=255)                                                                                             gtgGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCagcagc                                                      >  3:194688/1‑148 (MQ=255)                                                                                               ggaggaCCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCCGGCCAGAAAGCACGCCCGCGGGAGCATCTGCCCCCACCCCACCCTCCTGCGGCGCAAGCCCCAGCAGCTg                                                    >  1:398301/1‑148 (MQ=255)                                                                                               ggaggaCCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTg                                                    >  4:246178/1‑148 (MQ=255)                                                                                                       aaaTCGACCTCATTCAGGCGCGGTGTATCTGCCGGAGCGTTAACCGCGATGACATGACAACCCGCCGCCAGTCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTa                                            <  1:111867/148‑1 (MQ=255)                                                                                                       aaaTCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCCCCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTa                                            <  6:1363/148‑1 (MQ=255)                                                                                                       aaaTCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCt                                             >  8:267117/1‑147 (MQ=255)                                                                                                            gACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAg                                        <  4:354595/147‑1 (MQ=255)                                                                                                              ccTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATa                                     >  3:116650/1‑148 (MQ=255)                                                                                                                tCATTCCGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCAGCGCGCCTAACAGATACg                                   >  6:9414/1‑148 (MQ=255)                                                                                                                tCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAACGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCGCAGCAGCTGCGCGCCTAACAGATACg                                   >  3:21328/1‑148 (MQ=255)                                                                                                                tCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTcc                                                                                          >  1:89794/1‑93 (MQ=255)                                                                                                                tCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTcc                                                                                          <  2:89794/93‑1 (MQ=255)                                                                                                                tCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACg                                   >  8:56704/1‑148 (MQ=255)                                                                                                                tCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACg                                   >  7:295751/1‑148 (MQ=255)                                                                                                                  aTTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACg                                   <  6:298204/146‑1 (MQ=255)                                                                                                                     cAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATc                               <  3:246178/147‑1 (MQ=255)                                                                                                                     cAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATc                               <  2:93069/147‑1 (MQ=255)                                                                                                                                      ccGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTcc                <  6:212890/145‑1 (MQ=255)                                                                                                                                          aGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTc         <  7:267117/148‑1 (MQ=255)                                                                                                                                               tAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACcacac                                                                                <  7:359030/72‑1 (MQ=255)                                                                                                                                               tAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACcacac                                                                                >  8:359030/1‑72 (MQ=255)                                                                                                                                                aaCCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCTGGAGCATCTTCCACCCCCACACACTCCTGAGGCGCAAGCCCCATCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGc   >  7:179213/1‑148 (MQ=255)                                                                                                                                                aaCCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGc   >  5:134253/1‑148 (MQ=255)                                                                                                                                                   cGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCcagca                                                        >  2:406857/1‑92 (MQ=255)                                                                                                                                                   cGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCcagca                                                        <  1:406857/92‑1 (MQ=255)                                                                                                                                                   cGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCt  >  8:422422/1‑146 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGACAACCCGCCGCCAGGCCAGAAAGCACGCCAGCGGGAGCATCTTCCACCACCACACACTCCTGCGGCGCAAGCCCCAGCAGCTGCGCGCCTAACAGATACGCATCAGGTTCTGGTTTTCCGCGCTTCACTCGCT  >  NC_000913/2411404‑2411695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑