Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,224,192 | A→G | 5.4% | G520G (GGA→GGG) | ebgA → | evolved beta‑D‑galactosidase, alpha subunit |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,224,192 | 0 | A | G | 5.4% | 233.2 / 3.1 | 74 | G520G (GGA→GGG) | ebgA | evolved beta‑D‑galactosidase, alpha subunit |
Reads supporting (aligned to +/- strand): ref base A (27/43); new base G (2/2); total (29/45) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01 |
GGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGG > NC_000913/3224045‑3224335 | ggTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGa > 1:85677/1‑148 (MQ=255) gCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACcg > 2:307982/1‑148 (MQ=255) tACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACcgggcg > 7:206800/1‑148 (MQ=255) cGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACcgggcggg < 6:105112/148‑1 (MQ=255) cGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACcgggcgg < 7:50723/147‑1 (MQ=255) cGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACcgggcgg < 1:307982/147‑1 (MQ=255) agaagaTCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCt > 8:239351/1‑147 (MQ=255) agaagaTCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCt > 4:292268/1‑147 (MQ=255) agaacaCCGCTATGCTCAAGTGGTCGATATTATTTCCACCATCTACACCCGCGAGCCGCTGAGGACTGAGTTTGGTGAATACCCGCATCCGAATCCGCGCATCATCTGTGAATATGCTCATCCGATGGGGAACGGACCGGGCGGGCTg < 4:106375/148‑1 (MQ=255) gaagaTCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGGGTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATCTGCTCATGCGATGGGGAACGGACCGGGCGGGCTGa < 4:251322/148‑1 (MQ=255) agaTCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCACATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGAc < 6:227683/147‑1 (MQ=255) gaTCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACgg < 7:367615/148‑1 (MQ=255) gcgATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGt < 6:423044/147‑1 (MQ=255) gcgATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGAGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTa > 3:33483/1‑148 (MQ=255) gATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTAc > 1:189830/1‑147 (MQ=255) aGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACgg < 2:256503/134‑1 (MQ=255) aGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACgg > 1:256503/1‑134 (MQ=255) gTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGtct < 3:61973/148‑1 (MQ=255) tCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCta < 7:29701/148‑1 (MQ=255) tCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCta < 4:14780/148‑1 (MQ=255) cGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCta > 1:257143/1‑147 (MQ=255) gATATTATTTCCACCATGTACACCCGCTTGCCGCTGATTAATGAGTTTGTTGAATAGCCGCATCCGAAGCCGAGCATCATCTGTTAATTTGCTGATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTAGCAGAACGTCTTCtata < 2:96436/148‑1 (MQ=255) atatTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACgg < 4:184119/126‑1 (MQ=255) atatTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACgg > 3:184119/1‑126 (MQ=255) atatTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCtat > 1:13485/1‑146 (MQ=255) tattatTTCCACCATGTACACCCGCGTGCCGATGATTAATGAGTTTGGTGATTACCCGCATCCGAAGCCGGGCTTCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATaa < 7:99389/147‑1 (MQ=255) ttattTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGc < 2:85677/147‑1 (MQ=255) tttCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGa < 7:239351/148‑1 (MQ=255) tCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGAt < 2:110168/147‑1 (MQ=255) caccaTGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGc < 7:17922/148‑1 (MQ=255) caccaTGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGc < 2:7262/148‑1 (MQ=255) ccaTGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCAt > 7:434261/1‑148 (MQ=255) caTGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACAGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAATCAAGATTGCag > 4:325031/1‑146 (MQ=255) tGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCa < 7:37668/148‑1 (MQ=255) tGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCa < 7:253322/148‑1 (MQ=255) tACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAg < 3:346293/147‑1 (MQ=255) cacCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCtata > 7:281720/1‑128 (MQ=255) cacCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCtata < 8:281720/128‑1 (MQ=255) cgcgTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCAtt < 8:103743/148‑1 (MQ=255) tGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATGAGCACGATTGCATTCAGGGTCATTATGt > 4:154597/1‑148 (MQ=255) tGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGt < 8:434261/148‑1 (MQ=255) gCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTc > 8:235656/1‑148 (MQ=255) ccGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATAATCTCTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCt < 8:87477/148‑1 (MQ=255) cTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTggg < 3:356879/148‑1 (MQ=255) cTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTggg < 7:235656/148‑1 (MQ=255) aGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCg < 2:189830/147‑1 (MQ=255) tttGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGAc > 7:89555/1‑147 (MQ=255) aaTACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGAt < 5:78645/148‑1 (MQ=255) tACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAgtg < 3:174998/131‑1 (MQ=255) tACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAgtg > 4:174998/1‑131 (MQ=255) cccGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCa < 3:292268/147‑1 (MQ=255) cGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCCGGGTCATTATGTCTGGGCGTGGTGCGACCCCGGGATCCAgg > 3:215944/1‑147 (MQ=255) catcatCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACgg > 6:412797/1‑147 (MQ=255) tcatcTGTGAATATGCTCATGCGATGGGGAACGGGCCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAg > 2:215317/1‑107 (MQ=255) tcatcTGTGAATATGCTCATGCGATGGGGAACGGGCCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAg < 1:209264/107‑1 (MQ=255) tcatcTGTGAATATGCTCATGCGATGGGGAACGGGCCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAg < 1:215317/107‑1 (MQ=255) tcatcTGTGAATATGCTCATGCGATGGGGAACGGGCCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAg > 2:209264/1‑107 (MQ=255) tcatcTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCATAACGGCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTTTTGCGACCACGGGATCCAGGCACAGTACGACCACGGCaa < 4:33483/148‑1 (MQ=255) catcTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAATCACGATTGCATTAAGGGTCATTATGTCTGGGAGTGGTGCGACCACTGGATCCAGGCACAGGACGACCACGGCaa > 7:379882/1‑147 (MQ=255) catcTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCt < 4:154744/101‑1 (MQ=255) catcTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCt > 3:154744/1‑101 (MQ=255) catcTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAAt > 4:211422/1‑148 (MQ=255) atcTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATg > 7:190099/1‑148 (MQ=255) cTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTc > 1:9196/1‑148 (MQ=255) tgAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCt < 2:257143/146‑1 (MQ=255) tgAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCt < 8:206800/146‑1 (MQ=255) gAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTgg > 3:274149/1‑147 (MQ=255) aaTATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGt < 8:190099/147‑1 (MQ=255) atGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCtat < 7:332111/59‑1 (MQ=255) atGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCtat > 8:332111/1‑59 (MQ=255) tCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTc < 7:190225/148‑1 (MQ=255) cGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTcggcgg < 6:428434/148‑1 (MQ=255) aTGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTc < 3:163184/129‑1 (MQ=255) aTGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTc > 4:163184/1‑129 (MQ=255) aCGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGcggcgactacgg < 2:13485/148‑1 (MQ=255) | GGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGG > NC_000913/3224045‑3224335 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |