New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2276138 =41 (1.040)6 (0.160) 6/254 3.6 13.4% coding (866/1026 nt) yejE microcin C ABC transporter permease
?NC_000913 2276159 = 39 (1.050)coding (887/1026 nt) yejE microcin C ABC transporter permease
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGA  >  NC_000913/2276011‑2276303
                                                                                                                                                    |                                                                                                                                                
aGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAAct                                                                                                                                                  >  2:1199/1‑149 (MQ=255)
         cTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTAc                                                                                                                                          <  5:59020/148‑1 (MQ=255)
            cGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAg                                                                                                                                       <  7:143885/148‑1 (MQ=255)
                     ttGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGt                                                                                                                                             >  8:119220/1‑133 (MQ=255)
                     ttGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGt                                                                                                                                             <  7:119220/133‑1 (MQ=255)
                       gCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAAcc                                                                                                                           >  5:147449/1‑149 (MQ=255)
                          tAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCtt                                                                                                                         >  5:145107/1‑148 (MQ=255)
                            aTGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCa                                                                                                                       <  6:145107/148‑1 (MQ=255)
                               ccATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGcc                                                                                                                   >  8:136229/1‑149 (MQ=255)
                               ccATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGcc                                                                                                                   >  2:155605/1‑149 (MQ=255)
                                 aTGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGcccc                                                                                                                 <  6:147449/149‑1 (MQ=255)
                                        cgACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCtt                                                                                                          <  7:136229/149‑1 (MQ=255)
                                          aCCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTc                                                                                                                        <  6:90965/133‑1 (MQ=255)
                                          aCCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTc                                                                                                                        >  5:90965/1‑133 (MQ=255)
                                              tCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTTCAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGAt                                                                                                     >  7:73908/1‑148 (MQ=255)
                                              tCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTAc                                                                                                                                          >  2:155885/1‑111 (MQ=255)
                                              tCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGcccc                                                                                                                 <  6:8501/136‑1 (MQ=255)
                                              tCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGcccc                                                                                                                 >  5:8501/1‑136 (MQ=255)
                                              gcACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCCCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATACCCTTCAGGCCCCGTGGCTTGGGATc                                                                                                    >  7:73909/2‑149 (MQ=255)
                                                           gTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGt                                                                                       >  4:87196/1‑149 (MQ=255)
                                                                         gTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGAtatt                                                                          >  2:197141/1‑148 (MQ=255)
                                                                                 accaccCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCttt                                                                 <  5:31491/149‑1 (MQ=255)
                                                                                    accCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCt                                                              >  7:149856/1‑149 (MQ=255)
                                                                                      ccTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGa                                                                              <  5:156826/131‑1 (MQ=255)
                                                                                      ccTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGa                                                                              >  6:156826/1‑131 (MQ=255)
                                                                                      ccTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTc                                                                    <  4:35256/141‑1 (MQ=255)
                                                                                      ccTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTc                                                                    >  3:35256/1‑141 (MQ=255)
                                                                                          aCCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCt                                                         <  1:155605/148‑1 (MQ=255)
                                                                                             gcgcgcgATTTCCTCGGCTTCGGGCTACCGCTCGGTTCACAGTCACTAGGTGAACTGCGGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCGTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTAt                                                     <  8:73909/148‑1 (MQ=255)
                                                                                               gctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTTCAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTAt                                                     <  8:73908/147‑1 (MQ=255)
                                                                                               gctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTg                                                   <  3:87196/149‑1 (MQ=255)
                                                                                               gctcgATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTACTGTCTTTGCTGATCTTTAt                                                     <  1:197141/147‑1 (MQ=255)
                                                                                                   gATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATTACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTg                                                >  2:17957/1‑148 (MQ=255)
                                                                                                   gATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGt                                                 <  1:1199/147‑1 (MQ=255)
                                                                                                   gATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTg                                                >  4:151246/1‑148 (MQ=255)
                                                                                                        ccTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATattatt                                                                       >  4:114558/1‑120 (MQ=255)
                                                                                                        ccTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATattatt                                                                       <  3:114558/120‑1 (MQ=255)
                                                                                                                 cGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGAc                                  <  8:149856/148‑1 (MQ=255)
                                                                                                                                       tCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCACCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGgt           <  4:31298/149‑1 (MQ=255)
                                                                                                                                             ggTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGa                                                                              <  7:95149/76‑1 (MQ=255)
                                                                                                                                             ggTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGa                                                                              >  8:95149/1‑76 (MQ=255)
                                                                                                                                               tGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCAt    >  5:11148/1‑148 (MQ=255)
                                                                                                                                                gAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGa  <  3:151246/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AGTATCATCCTGCGTCATATGTTGCCTAATGCCATGGTCGCGACCCTCACCTTTTTACCGTTTATTTTATGTAGTTCGATAACCACCCTGACCTCGCTCGATTTCCTCGGCTTCGGTCTACCGCTCGGTTCACCGTCACTCGGTGAACTGCTGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGA  >  NC_000913/2276011‑2276303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.