Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	757,572	C→T	H556Y (CAC→TAC)	sdhA →	succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	757,572	0	C	T	100.0%	65.9 / NA	20	H556Y (CAC→TAC)	sdhA	succinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/9);  total (11/9)

GAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGC  >  NC_000913/757440‑757715                                                                                                                                     |                                                                                                                                                gAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGc                                                                                                                                  <  6:141835/148‑1 (MQ=255)                     ccTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGCGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGAcgc                                                                                                             >  6:90713/1‑149 (MQ=255)                          tGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCg                                                                                                         >  1:2875/1‑148 (MQ=255)                              aaCGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCg                                                                                                    >  8:70476/1‑149 (MQ=255)                                  gCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCTTCGGCGCGTCat                                                                                                <  1:101229/149‑2 (MQ=255)                                               cTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGaaa                                                                                   <  2:2875/149‑1 (MQ=255)                                                      tGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGcc                                                                            >  1:149297/1‑149 (MQ=255)                                                        ccAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGcc                                                                            <  7:70476/147‑1 (MQ=255)                                                                           ccGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGCATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTc                                                       >  5:175274/1‑149 (MQ=255)                                                                              tGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGTCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGta                                                    <  2:149297/149‑1 (MQ=255)                                                                                                    ttCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGa                               >  4:25649/1‑148 (MQ=255)                                                                                                    ttCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGa                               >  6:33450/1‑148 (MQ=255)                                                                                                      cccGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGaaa                             <  3:25340/148‑1 (MQ=255)                                                                                                      cccGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGa                               <  5:33450/146‑1 (MQ=255)                                                                                                                    atgaAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGttt              >  4:90443/1‑149 (MQ=255)                                                                                                                      gaAAACTGGCTGTGCTACTCCCTGCATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGtttt             <  6:175274/148‑1 (MQ=255)                                                                                                                        aaaCTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCaa          >  4:137532/1‑149 (MQ=255)                                                                                                                         aaCTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCg                                        >  5:82848/1‑118 (MQ=255)                                                                                                                         aaCTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCg                                        <  6:82848/118‑1 (MQ=255)                                                                                                                                tgtgCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGc  >  8:148425/1‑149 (MQ=255)                                                                                                                                     |                                                                                                                                                GAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGC  >  NC_000913/757440‑757715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑