Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,285,719	Δ1 bp	5.5%	coding (1653/1959 nt)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,719	0	C	.	5.5%	246.5 / 7.2	73	coding (1653/1959 nt)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (36/33);  new base . (2/2);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

GTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACG  >  NC_000913/4285572‑4285865                                                                                                                                                    |                                                                                                                                                   gTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCg                                                                                                                                                   <  5:74145/149‑1 (MQ=255)  tCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCgg                                                                                                                                                  <  6:162601/149‑1 (MQ=255)    ggTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGaa                                                                                                                                                <  6:256477/149‑1 (MQ=255)        cAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATAcc                                                                                                                                            >  8:160929/1‑149 (MQ=255)         aGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGGTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCGCCCCGTGATTAAGCGTGACGTAGGCGTAGCTCGCCTGACCTGTAATATTGTGCGGAATAcc                                                                                                                                            >  5:212781/1‑148 (MQ=255)          gTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACct                                                                                                                                           >  8:212092/1‑148 (MQ=255)           tGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACctac                                                                                                                                         >  2:39725/1‑149 (MQ=255)                caCGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTAcgg                                                                                                                                    >  4:287455/1‑149 (MQ=255)                caCGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTAcgg                                                                                                                                    >  4:282720/1‑149 (MQ=255)                 aCGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTAcggc                                                                                                                                   <  1:286653/149‑1 (MQ=255)                   gTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTAcggcgg                                                                                                                                 <  8:112163/149‑1 (MQ=255)                    tCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACggcggc                                                                                                                                <  1:23830/149‑1 (MQ=255)                        gcggcgCCAGCGGGCCAATCTCTTTACGCCCCCACTTGCGGACTTCTGCGTACAGTTCGGGTGCCGGGTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGCTCGCCTGACCTTTACTATTGTGCGGACTACCTACTACGGCGGCTTCg                                                                                                                            <  4:241475/149‑1 (MQ=255)                        gCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCTGAATACCTACTACGGCGGCttg                                                                                                                             <  7:240060/148‑2 (MQ=255)                        gCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCg                                                                                                                            <  6:7646/149‑1 (MQ=255)                        gCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTGCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTc                                                                                                                             <  4:262349/148‑1 (MQ=255)                         cGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCgg                                                                                                                           <  7:212092/149‑1 (MQ=255)                             gCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAAt                                                                                                                       >  5:50224/1‑149 (MQ=255)                             gCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAAt                                                                                                                       >  8:199856/1‑149 (MQ=255)                                     gCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGCCCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGAt                                                                                                               >  5:318093/1‑149 (MQ=255)                                         aTCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATgcgc                                                                                                           >  4:289461/1‑149 (MQ=255)                                             ctTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGccac                                                                                                        >  2:341077/1‑148 (MQ=255)                                                  cGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAgcgc                                                                                                  <  5:114302/149‑1 (MQ=255)                                                       ccAGTTGCGGACTTCTGCGTGCAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGCAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACt                                                                                             >  8:142497/1‑149 (MQ=255)                                                           ttGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAAt                                                                                         <  1:341077/149‑1 (MQ=255)                                                           ttGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAAt                                                                                         >  7:76809/1‑149 (MQ=255)                                                                   ttCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCg                                                                                 >  8:342241/1‑149 (MQ=255)                                                                     cTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTc                                                                               >  6:177884/1‑149 (MQ=255)                                                                         gTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCa                                                                           <  5:177884/149‑1 (MQ=255)                                                                               ttCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGt                                                                     <  1:39725/149‑1 (MQ=255)                                                                               ttCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCATTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGt                                                                     >  4:90280/1‑149 (MQ=255)                                                                                    gTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATgcgc                                                                                                           <  4:283104/106‑1 (MQ=255)                                                                                    gTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATgcgc                                                                                                           >  3:283104/1‑106 (MQ=255)                                                                                    gTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCg                                                                >  8:56807/1‑149 (MQ=255)                                                                                    gTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCg                                                                <  3:289461/149‑1 (MQ=255)                                                                                    gTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCg                                                                <  4:48008/149‑1 (MQ=255)                                                                                     tGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCgg                                                               <  6:50224/149‑1 (MQ=255)                                                                                       aCGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCg                                                                >  5:91828/1‑146 (MQ=255)                                                                                       aCGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGgag                                                             >  6:178025/1‑149 (MQ=255)                                                                                       aCGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGT‑‑GGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGcc                                                                                  <  5:272610/126‑1 (MQ=255)                                                                                       aCGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGT‑‑GGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGcc                                                                                  >  6:272614/1‑126 (MQ=255)                                                                                       aCGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGT‑‑GGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGcc                                                                                  >  6:272610/1‑126 (MQ=255)                                                                                       aCGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGT‑‑GGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGcc                                                                                  <  5:272614/126‑1 (MQ=255)                                                                                        cGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGgaga                                                            <  7:342241/149‑1 (MQ=255)                                                                                          gTTCCTCCCCGTGATTAAGCGTGCCGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACg                                                          >  5:159636/1‑149 (MQ=255)                                                                                              ctccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTc                                                       <  3:209845/148‑1 (MQ=255)                                                                                               tccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGAcc                                                                 <  4:146763/137‑1 (MQ=255)                                                                                               tccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGAcc                                                                 >  3:146763/1‑137 (MQ=255)                                                                                               tccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAg                                                     >  5:19824/1‑149 (MQ=255)                                                                                                 cccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCa                                                   <  6:91828/149‑1 (MQ=255)                                                                                                 cccGTGATTAAGCGTGAAGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCa                                                   >  5:3631/1‑149 (MQ=255)                                                                                                           aGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCca                                          >  6:87489/1‑148 (MQ=255)                                                                                                            gCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCcacc                                        >  6:213165/1‑148 (MQ=255)                                                                                                             cGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCacac                                       <  3:282720/149‑1 (MQ=255)                                                                                                             cGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCacac                                       <  3:287455/149‑1 (MQ=255)                                                                                                                gACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGa                                                                         >  6:119737/1‑112 (MQ=255)                                                                                                                  cGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGtt                                                        >  6:307090/1‑127 (MQ=255)                                                                                                                  cGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGtt                                                        <  5:307090/127‑1 (MQ=255)                                                                                                                     aGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGTCTTCGTCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGt                                >  2:245141/1‑148 (MQ=255)                                                                                                                       gCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTAt                             <  5:87489/149‑1 (MQ=255)                                                                                                                        cGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATc                            <  5:178025/149‑1 (MQ=255)                                                                                                                         gTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATcc                           <  6:3631/149‑1 (MQ=255)                                                                                                                             aTCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGt                        >  3:23046/1‑148 (MQ=255)                                                                                                                                     aCCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCAt               >  6:305384/1‑149 (MQ=255)                                                                                                                                     aCCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCAt               >  4:112200/1‑149 (MQ=255)                                                                                                                                        tttAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAAt                     >  2:240015/1‑140 (MQ=255)                                                                                                                                        tttAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAAt                     <  1:240015/140‑1 (MQ=255)                                                                                                                                        tttAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAg                   >  4:175122/1‑142 (MQ=255)                                                                                                                                        tttAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAg                   <  3:175122/142‑1 (MQ=255)                                                                                                                                            atatTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATc        <  7:152456/149‑1 (MQ=255)                                                                                                                                            atatTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATc        <  7:160929/149‑1 (MQ=255)                                                                                                                                               ttGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATcgc      >  1:159095/1‑148 (MQ=255)                                                                                                                                                  tgCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGCCTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAcg  >  6:70665/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACG  >  NC_000913/4285572‑4285865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑