Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,733,247 C→T 5.4% D349D (GAC→GAT)  xylG → D‑xylose ABC transporter dual domain ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,733,2470CT 5.4% 237.3 / 2.4 74D349D (GAC→GAT) xylGD‑xylose ABC transporter dual domain ATPase
Reads supporting (aligned to +/- strand):  ref base C (40/30);  new base T (2/2);  total (42/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

CATTCAGTGCCTGTTTGGTGTGTGGCCCGGACAATGGGAAGGAAAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCC  >  NC_000913/3733109‑3733394
                                                                                                                                          |                                                                                                                                                   
cATTCAGTGCCTGTTTGGTGTGTGGCCAGGACAAAGGTAATGAAAAATTTATATTGATGGCAAACAGGTAGATATTGGTAACTGTCATAAAGCAATAGCCCATGGGATTGCGTTGGTCCCAGAAGCCATAAAGCGCGACGGCATCGTTc                                                                                                                                           >  7:239707/1‑149 (MQ=255)
 aTTCAGTGCCTGTTTGGTGTGTGGCCCGGACAATGGGAAGGAAAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTcc                                                                                                                                          >  6:245961/1‑149 (MQ=255)
   tCAGTGCCTGTTTGGTGTGTGGCCCGGACAATGGGAAGGAAAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCgg                                                                                                                                        >  5:320082/1‑149 (MQ=255)
         ccTGTTTGGTGTGTGGCCCGGACAATGGGAAGGAAAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCCAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAATCGCGACGGCCTCGTTCCGGTAATgt                                                                                                                                  >  5:167870/1‑148 (MQ=255)
         ccTGTTTGGTGTGTGGCCCGGACAATGGGAAGGAAAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATgg                                                                                                                                  >  3:284160/1‑149 (MQ=255)
            gTTTGGTGTGTGGCCCGGACAATGGGAAGGAAAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGATGGCATCGTTCCGGTAATGGCgg                                                                                                                               >  6:186125/1‑149 (MQ=255)
             tttGGTGTGTGGCCCGGACAATGGGAAGGAAAAATTTATATTGATGGCAAACAGGTAGATATACGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGt                                                                                                                              >  2:150621/1‑149 (MQ=255)
               tGGTGTGTGGCCCGGACAATGGGAAGGAAAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGtt                                                                                                                             <  4:242929/148‑1 (MQ=255)
                     gtgGCCCGGACAATGGGAAGGAAAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTaaa                                                                                                                       >  8:221894/1‑148 (MQ=255)
                        gCCCGGACAATGGGAAGGAAAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAata                                                                                                                   >  1:117315/1‑149 (MQ=255)
                          ccGGACAATGGGAAGGAAAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATAtt                                                                                                                 <  8:328218/149‑1 (MQ=255)
                              aCAATGGGAAGGAAAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTAccc                                                                                                             >  8:77605/1‑149 (MQ=255)
                                aaTGGGAAGGAAAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTc                                                                                                           >  3:280892/1‑149 (MQ=255)
                                    ggaaggaaAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTcgcc                                                                                                        <  5:245961/148‑1 (MQ=255)
                                    ggaaggaaAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTcgcc                                                                                                        <  7:256324/148‑1 (MQ=255)
                                       aggaaAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCAc                                                                                                    <  6:320082/149‑1 (MQ=255)
                                       aggaaAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCAc                                                                                                    <  3:187125/149‑1 (MQ=255)
                                       aggaaAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCAc                                                                                                    >  1:234827/1‑149 (MQ=255)
                                         gaaAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACt                                                                                                   >  7:25418/1‑148 (MQ=255)
                                          aaaaaTTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCTCCCAGGGGATTGCGATGGTCCCCGAAGAATGAAAGCGCGACGGCATCGTTCCTGTAATGGCGGTTGGTAAAAATATTTCCCTCGCCGAACTc                                                                                                  >  1:314925/1‑148 (MQ=255)
                                               tttATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCACAGGCGATTGCGATGGTACAAGAAGACAGAAAGCGACACGGCATCGTTACGGTAATGTCGGTTGGAAAAAATATTACCCTCGCCGCACTCAATaaa                                                                                            >  7:121764/1‑149 (MQ=255)
                                                     ttGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTAcc                                                                                      >  7:205069/1‑149 (MQ=255)
                                                     ttGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTAc                                                                                       <  2:234827/148‑1 (MQ=255)
                                                      tGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCg                                                                                     >  6:16429/1‑149 (MQ=255)
                                                         tGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGTGCGATGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTg                                                                                  >  1:273676/1‑149 (MQ=255)
                                                           gCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTgg                                                                                 >  3:129783/1‑148 (MQ=255)
                                                           gCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTgg                                                                                 >  4:131606/1‑148 (MQ=255)
                                                               aCAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTa                                                                            <  2:140196/149‑1 (MQ=255)
                                                                   gTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGc                                                                          >  8:143574/1‑147 (MQ=255)
                                                                   gTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGc                                                                          >  6:21187/1‑147 (MQ=255)
                                                                   gTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGc                                                                          >  5:120682/1‑147 (MQ=255)
                                                                   gTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCa                                                                        >  6:260634/1‑149 (MQ=255)
                                                                   gTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCa                                                                        <  8:221425/149‑1 (MQ=255)
                                                                                aCTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGTGCGATGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGAcgc                                                            <  2:273676/148‑1 (MQ=255)
                                                                                aCTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGAcgc                                                            <  5:16429/148‑1 (MQ=255)
                                                                                    tCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCa                                                        <  4:280892/148‑1 (MQ=255)
                                                                                      aGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGAcgc                                                            >  6:286856/1‑142 (MQ=255)
                                                                                      aGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGAcgc                                                            <  5:286856/142‑1 (MQ=255)
                                                                                       gCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGAATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACACTCGCAACACTCAATAAATTTACAGATCGCATTATCCAGCAAGATGACGCGGCagag                                                     >  2:304912/1‑148 (MQ=255)
                                                                                        cAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGc                                                    >  2:235661/1‑148 (MQ=255)
                                                                                          aGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCaa                                                  >  2:130150/1‑148 (MQ=255)
                                                                                          aGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCAGCACTCAATAAATTTACCGGTGGCATTATCCAGCTTGATGACTCGGCAGAGCaa                                                  >  4:205773/1‑148 (MQ=255)
                                                                                             cATCGCCCATGTGTTTGCGATTGTCCCCGCATACATAAAGCGCGACGTCATCGTTGCGGGAATTGCGGGTGGTAAAAATATTACCCTCGCCGCACTCAAAAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAAt                                              <  8:239707/149‑1 (MQ=255)
                                                                                             cATCGCCCAGGGGATTGCGATGGTCCCGGAAGACAGAAAGCGCGACGGTATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAAt                                              >  7:20967/1‑149 (MQ=255)
                                                                                             cATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAAt                                              <  1:235661/149‑1 (MQ=255)
                                                                                             cATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAAt                                              <  7:221894/149‑1 (MQ=255)
                                                                                             cATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAAt                                              >  7:108693/1‑149 (MQ=255)
                                                                                               tCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGt                                            <  4:129783/149‑1 (MQ=255)
                                                                                                 gCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTa                                           <  7:77605/148‑1 (MQ=255)
                                                                                                 gCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTAt                                          >  5:113505/1‑149 (MQ=255)
                                                                                                  cccAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTAtt                                         <  2:117315/149‑1 (MQ=255)
                                                                                                   ccAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTg                                                                                  >  2:262823/1‑107 (MQ=255)
                                                                                                   ccAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTg                                                                                  <  1:262823/107‑1 (MQ=255)
                                                                                                    cAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCt                                       <  5:21187/149‑1 (MQ=255)
                                                                                                    cAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCt                                       >  8:120029/1‑149 (MQ=255)
                                                                                                     aGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTg                                      <  8:25418/149‑1 (MQ=255)
                                                                                                     aGGGGATTGCGATGGTCCCCGAAGACAGAAAGAGCGACGGCATCGTTCCGGTAATGGCGGTTTGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTg                                      <  6:167870/149‑1 (MQ=255)
                                                                                                     aGGGGATTGCGATGGTCACCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTg                                      <  8:121764/149‑1 (MQ=255)
                                                                                                        ggATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGaa                                   <  1:150621/149‑1 (MQ=255)
                                                                                                              cGATGGTCCCCGAAGACAGAAAGCGCGATGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGaatcaat                              <  5:186125/148‑1 (MQ=255)
                                                                                                              cGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGaatcaat                              <  7:143574/148‑1 (MQ=255)
                                                                                                               gATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTTGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATcc                            <  4:284160/149‑1 (MQ=255)
                                                                                                               gATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATcc                            <  5:260634/149‑1 (MQ=255)
                                                                                                                       ccGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCaa                       >  6:71820/1‑146 (MQ=255)
                                                                                                                       ccGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCaa                       <  5:71820/146‑1 (MQ=255)
                                                                                                                       ccGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAAc                      <  7:71637/147‑1 (MQ=255)
                                                                                                                       ccGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAAc                      >  8:71637/1‑147 (MQ=255)
                                                                                                                       ccGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACt                     >  8:151437/1‑148 (MQ=255)
                                                                                                                              cAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGtt              >  2:67377/1‑148 (MQ=255)
                                                                                                                               aGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTa             >  1:131115/1‑148 (MQ=255)
                                                                                                                                gAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTaaa           <  1:130150/149‑1 (MQ=255)
                                                                                                                                 aaaGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGAAGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTaaaa          >  6:274157/1‑149 (MQ=255)
                                                                                                                                        gACGGCATCGATCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACgtcgtc   >  2:221771/1‑149 (MQ=255)
                                                                                                                                         aCGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTcc  >  4:266165/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
CATTCAGTGCCTGTTTGGTGTGTGGCCCGGACAATGGGAAGGAAAAATTTATATTGATGGCAAACAGGTAGATATTCGTAACTGTCAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCTGGAATCAATCCAGCAACTCAAAGTTAAAACGTCGTCC  >  NC_000913/3733109‑3733394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑