Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,792,766 A→T 6.2% intergenic (‑215/+60) kbl ← / ← yibB glycine C‑acetyltransferase/YibB family protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,792,7660AT 6.2% 330.6 / 8.2 107intergenic (‑215/+60)kbl/yibBglycine C‑acetyltransferase/YibB family protein, function unknown
Reads supporting (aligned to +/- strand):  ref base A (50/50);  new base T (3/4);  total (53/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 113 total aligned reads displayed.

GCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTC  >  NC_000913/3792619‑3792914
                                                                                                                                                   |                                                                                                                                                    
gcgTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCa                                                                                                                                                      >  3:161843/1‑148 (MQ=255)
gcgTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCa                                                                                                                                                      >  2:195431/1‑148 (MQ=255)
 cgTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCaaa                                                                                                                                                    <  8:27058/149‑1 (MQ=255)
 cgTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCaaa                                                                                                                                                    <  1:124238/149‑1 (MQ=255)
     aaCACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGca                                                                                                                                                 <  3:118360/148‑1 (MQ=255)
      acacTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGcat                                                                                                                                                >  8:316789/1‑148 (MQ=255)
          ttCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAttt                                                                                                                                           >  1:134356/1‑149 (MQ=255)
            cTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCAtttt                                                                                                                                          <  3:29763/148‑1 (MQ=255)
             tGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTc                                                                                                                                        >  6:53728/1‑149 (MQ=255)
               cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAttttttc                                                                                                                                       <  4:25790/148‑3 (MQ=255)
               cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCATTTTTTCt                                                                                                                                      <  2:134356/149‑1 (MQ=255)
               cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTca                                                                                                                                       >  4:246691/1‑147 (MQ=255)
               cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCt                                                                                                                                      <  4:205900/149‑1 (MQ=255)
                aaaaTTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTa                                                                                                                                     >  3:249392/1‑149 (MQ=255)
                  aaTTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGCTTTGGCTGCGCCTAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTAtc                                                                                                                                   <  7:238679/149‑1 (MQ=255)
                   aTTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTAtct                                                                                                                                  >  7:189269/1‑149 (MQ=255)
                         gATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTg                                                                                                                            >  7:34292/1‑149 (MQ=255)
                           taaataaCGTGTACCTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTAGCCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGtttg                                                                                                                           >  6:314207/1‑146 (MQ=255)
                           taaataaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGt                                                                                                                          <  1:195431/149‑1 (MQ=255)
                           taaataaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGt                                                                                                                          <  4:161843/149‑1 (MQ=255)
                             aataaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTTGTa                                                                                                                         >  3:179613/1‑148 (MQ=255)
                             aataaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTa                                                                                                                         <  7:287005/148‑1 (MQ=255)
                                  agtgtAACTTGATGTCTCTCAACAACTACAGATAGTAGAAAAACTGTACAGATTTGGCTGCGCATCTAGTTGATTGATTTTACTGCATTGATATAAAAAGAAATATTGATCCCAAAGCATCATTTTTCCTATCTCAGTTTGAATCaaat                                                                                                                   <  2:147333/148‑2 (MQ=255)
                                   gtgtAACTTGATGTCTCTCAACACCTACATTTATTATAAAAACTGTACAGTTTTTGCTGGGCATAATGATTATTGATTTTTCTGCATTGATATAAAAATAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAAc                                                                                                                  <  5:187311/149‑1 (MQ=255)
                                   gtgtAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAAc                                                                                                                  <  8:58701/149‑1 (MQ=255)
                                   gtgtAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAAc                                                                                                                  <  5:333862/149‑1 (MQ=255)
                                     gtAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGc                                                                                                                >  2:328748/1‑149 (MQ=255)
                                        acaagATGTCTCTCAACACCTACAGATACTAGAAAAACTGAACACTTTTGGCTCGGCATAAAGATGATAGATTTTACTGCATTGATATAAAAAGAAAAAAAGAAGCCAAAGCATGATTTATCCTATCTCAGTTGGTATCAAAACGCAAc                                                                                                             >  4:209257/5‑149 (MQ=255)
                                        aCTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                              <  2:64745/148‑1 (MQ=255)
                                        aCTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                              <  4:301319/148‑1 (MQ=255)
                                          ttGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTAcc                                                                                                            >  8:208683/1‑148 (MQ=255)
                                             aTGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCAc                                                                                                         <  7:187324/148‑1 (MQ=255)
                                             aTGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATCATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCATATCTCAGTTGGTATCAAAACGCTACCCAc                                                                                                         >  6:305867/1‑148 (MQ=255)
                                                  tctcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGATGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAATAAAAAATGATGCCAACGCATCATTTTTCCTATCTCAGTTGTGATCAAAACGCTACCCACTAATg                                                                                                    >  5:208825/1‑148 (MQ=255)
                                                    tcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAACAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATg                                                                                                    <  1:223378/146‑1 (MQ=255)
                                                    tcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAttttttc                                                                                                                                       <  2:160192/111‑3 (MQ=39)
                                                    tcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAttttttc                                                                                                                                       >  1:160192/1‑109 (MQ=39)
                                                    tcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATg                                                                                                    <  5:71384/146‑1 (MQ=255)
                                                    tcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAg                                                                                                 >  7:256264/1‑149 (MQ=255)
                                                    tcAACAACTACATATATTATAAAAACTTTACAGTTTTGGATGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATg                                                                                                    <  1:160696/146‑1 (MQ=255)
                                                      aaCACCTACAGATAGTATAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                              <  5:173226/134‑1 (MQ=255)
                                                      aaCACCTACAGATAGTAGAATAACTGTACAGTTTTGGCTGGGAATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAg                                                                                                 <  6:64594/147‑1 (MQ=255)
                                                      aaCACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                              >  6:173226/1‑134 (MQ=255)
                                                      aaCACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAg                                                                                                 <  5:309194/147‑1 (MQ=255)
                                                           cTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAAc                                                                                                                  <  8:123984/125‑1 (MQ=255)
                                                           cTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAAc                                                                                                                  >  7:123984/1‑125 (MQ=255)
                                                               aGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTAtt                                                                                      >  3:136201/1‑149 (MQ=255)
                                                                 aTAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTc                                                                                                                                        >  2:273473/1‑97 (MQ=255)
                                                                 aTAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAAc                                                                                           >  1:145551/1‑142 (MQ=255)
                                                                 aTAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAAc                                                                                           <  2:145551/142‑1 (MQ=255)
                                                                 aTAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTAtttt                                                                                    <  7:208683/149‑1 (MQ=255)
                                                                  tagtagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTc                                                                                   <  8:34292/149‑1 (MQ=255)
                                                                    gtagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCtt                                                                                 <  8:290784/149‑1 (MQ=255)
                                                                    gtagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCtt                                                                                 <  7:316789/149‑1 (MQ=255)
                                                                        aaaaaCTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGa                                                                               >  4:236807/1‑147 (MQ=255)
                                                                         aaaaCTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAg                                                                            >  2:26147/1‑149 (MQ=255)
                                                                                 aCAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGTAAACCTATTTTCTTGAAAGGAAAATtc                                                                    >  7:288461/1‑149 (MQ=255)
                                                                                 aCAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATtc                                                                    >  7:45102/1‑149 (MQ=255)
                                                                                   aGTTTTGGCTTGGCATAATGAAGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATAATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATtctc                                                                  <  1:75177/149‑1 (MQ=255)
                                                                                   aGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATtctc                                                                  <  1:79799/149‑1 (MQ=255)
                                                                                    gTTTTGGCTGGTCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAACTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCa                                                                 <  7:340448/149‑1 (MQ=255)
                                                                                          gCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCt                                                           >  2:159536/1‑149 (MQ=255)
                                                                                          gCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCt                                                           <  5:78049/149‑1 (MQ=255)
                                                                                             gggCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCa                                                        >  2:75230/1‑149 (MQ=255)
                                                                                                    atgatgATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGc                                                 <  5:69214/149‑1 (MQ=255)
                                                                                                       atgatTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGcccc                                              >  5:252313/1‑149 (MQ=255)
                                                                                                       atgatTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGccc                                               <  5:12633/148‑1 (MQ=255)
                                                                                                           ttgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaaa                                          >  3:187708/1‑149 (MQ=255)
                                                                                                           ttgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaaa                                          <  5:53728/149‑1 (MQ=255)
                                                                                                              attTTACTGCATTGATATAAAAAGAAAAACTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGAATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtg                                       <  3:216844/149‑1 (MQ=255)
                                                                                                              attTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtg                                       >  7:331320/1‑149 (MQ=255)
                                                                                                               ttttACTGCATTGATATAAAAAGAAAAAATGATGCCTAAGCATCATTTTTCCTATCTCATTTTGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTATTGAAAGGAAAATTCTCAGCTCCTGCATTTTTGCCCCTAAAgtg                                       <  6:105167/148‑1 (MQ=255)
                                                                                                               ttttACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                      >  5:339661/1‑149 (MQ=255)
                                                                                                               ttttACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                      <  4:136201/149‑1 (MQ=255)
                                                                                                                  tACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAATGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTa                                    <  4:249392/148‑1 (MQ=255)
                                                                                                                   aCTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAACCCTATTTTCTTGAAAGGAAAATTCTCCGCGCCTGCCTTTTTGCCCCTAAAGTGTTACt                                  >  6:162702/1‑149 (MQ=255)
                                                                                                                       cATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTcc                                <  2:102592/147‑1 (MQ=255)
                                                                                                                        aTTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTg                              >  2:12424/1‑148 (MQ=255)
                                                                                                                                aaaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTCCCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCg                      >  8:265018/1‑148 (MQ=255)
                                                                                                                                aaaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTa                                    >  3:148032/1‑134 (MQ=255)
                                                                                                                                aaaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTa                                    <  4:148032/134‑1 (MQ=255)
                                                                                                                                 aaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGa                     >  3:118109/1‑148 (MQ=255)
                                                                                                                                  ataggaaaaaTGATGCTTTGGCATCATTTTTTCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGaaa                   >  5:75893/6‑149 (MQ=255)
                                                                                                                                   aaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTTTCAAACCGCTACCCACGAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTACAGTGTTAAGCCTGAAGCAGCGcaa                   >  4:222958/1‑148 (MQ=255)
                                                                                                                                   aaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAAc                  <  1:328748/149‑1 (MQ=255)
                                                                                                                                   aaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAAc                  <  6:252313/149‑1 (MQ=255)
                                                                                                                                      aaaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGGATCAAAACGCTACCCACTAATGGAGGGAACCCTAGTTTCTTGACAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCAAAAGTGTTACTCCTCAAGCAGAGAAACaa                >  5:215274/1‑148 (MQ=255)
                                                                                                                                       aaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAAt              <  5:239740/149‑1 (MQ=255)
                                                                                                                                        aaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATc             <  3:236807/149‑1 (MQ=255)
                                                                                                                                         aaaTGATGCCAACGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACACACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAATTGTTACTCCTGAAGCAGCGAAACaaagc             >  5:274908/1‑146 (MQ=255)
                                                                                                                                         aaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATc             >  3:32579/1‑148 (MQ=255)
                                                                                                                                          aaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCg            >  8:137245/1‑148 (MQ=255)
                                                                                                                                           atgatgCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGaa          <  6:339661/149‑1 (MQ=255)
                                                                                                                                           atgatgCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGaa          >  5:291309/1‑149 (MQ=255)
                                                                                                                                           atgatgCCAAAGCATCATTTTTCCGATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCGTGAAAGGAAATTTCTCAGCGCCTTCATTTTTGCCCCTAAAGTGTTACTCATGAAGCAGCGAAACAAATctac          >  5:260239/1‑146 (MQ=255)
                                                                                                                                                  cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAgcgc                                                             >  7:97053/1‑91 (MQ=255)
                                                                                                                                                  cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTc                                 >  5:234835/1‑119 (MQ=255)
                                                                                                                                                  cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTc                                 <  6:234835/119‑1 (MQ=255)
                                                                                                                                                  cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCAtttt   >  5:170298/1‑149 (MQ=255)
                                                                                                                                                   aaaGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTc  <  1:159536/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTC  >  NC_000913/3792619‑3792914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: