Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,828,279	G→T	5.6%	A129A (GCC→GCA)	gltS ←	glutamate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,828,279	0	G	T	5.6%	266.6 / 2.6	84	A129A (GCC→GCA)	gltS	glutamate transporter
Reads supporting (aligned to +/- strand):  ref base G (43/36);  new base T (3/2);  total (46/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

CCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGCCAACCACACGCCCACCGGCACGCAAACTGGCAATGTTGGCGTT  >  NC_000913/3828131‑3828425                                                                                                                                                     |                                                                                                                                                   ccGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCg                                                                                                                                                    <  3:149010/149‑1 (MQ=255) ccGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCg                                                                                                                                                    <  3:143933/149‑1 (MQ=255)      ccGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCaa                                                                                                                                               >  8:80239/1‑149 (MQ=255)       cGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAAc                                                                                                                                              >  4:274761/1‑149 (MQ=255)         ccAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAg                                                                                                                                            >  8:88613/1‑149 (MQ=255)           aaTCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGcc                                                                                                                                          <  2:230424/149‑1 (MQ=255)                   cgccCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGc                                                                                                                                  >  7:215312/1‑149 (MQ=255)                         gCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATc                                                                                                                             >  1:237145/1‑148 (MQ=255)                         gCACCAGACCGAACGTTGCACAGGCCATAGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATc                                                                                                                             >  6:63760/1‑148 (MQ=255)                            ccAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCaa                                                                                                                                               <  6:25173/127‑1 (MQ=255)                            ccAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCaa                                                                                                                                               >  5:25173/1‑127 (MQ=255)                            ccAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCAGTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCCGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGACACGGCCAACCGCCCCATCAGCggcacacgc                                                                                                                         >  1:213602/1‑142 (MQ=255)                             cAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCATTGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGcc                                                                                                                        <  1:348059/149‑1 (MQ=255)                             cAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGcccc                                                                                                                                        <  6:268448/133‑1 (MQ=255)                             cAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGcccc                                                                                                                                        >  5:268448/1‑133 (MQ=255)                             cAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGcc                                                                                                                        >  1:39863/1‑149 (MQ=255)                              aGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGccc                                                                                                                       <  2:237145/149‑1 (MQ=255)                              aGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGccc                                                                                                                       >  4:175224/1‑149 (MQ=255)                              aGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGccc                                                                                                                       <  7:88613/149‑1 (MQ=255)                               gcccgcccGTTGCACAGGCCCTCGCCACTGCCGTCGCATTGGTGAAGCCATACCGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCAGGAAAGATTAATAGAACCGGCCACCAGCCCCATACGCGGATCAAGccc                                                                                                                       >  6:290399/8‑148 (MQ=255)                                aCCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTTAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGGACCGTGACCGCCGGAAAGAGTAATAGAACCTGCCAACAGCCCCATCAGCGGATCAAGCCCt                                                                                                                      >  5:29786/1‑148 (MQ=255)                                aCCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCt                                                                                                                      >  4:162459/1‑148 (MQ=255)                                aCCGAACGTTGCACAGGCCATCGCCACTTCCCTCGCATTGGTGAAGCAATAACCTTCAATGAACAATTTACTCCACGCAGCGACCGTACCGTGACCGCCGGAAACAGTAATAGAACCGGCCAACACCCCCATCAGCGCATCAAGccat                                                                                                                      >  5:25319/1‑146 (MQ=255)                                  cGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCCCGCCGCGCCCGTCCCGTGCCCGCCGGAAAGAGTAATAGCACCGGCCGACAGCCCCATCAGCGGCTCAAGCGCTaa                                                                                                                    >  4:45241/1‑148 (MQ=255)                                    aaCGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACaa                                                                                                                 <  7:27978/149‑1 (MQ=255)                                              aGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTGACAAGCTAGCCATa                                                                                                       <  7:5031/149‑1 (MQ=255)                                              aGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATa                                                                                                       >  6:298627/1‑149 (MQ=255)                                              aGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATa                                                                                                       <  3:274761/149‑1 (MQ=255)                                                   aTCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATAccaat                                                                                                  <  7:80239/149‑1 (MQ=255)                                                   aTCGCAACTTCCGTCTCATTGGTGAAGACATAACGTTCAATGAAAAATTTACTCCACGAAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATAccaat                                                                                                  <  2:213602/149‑1 (MQ=255)                                                      gCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCTCGCAGCGCCCGTACCGTGACCGCCTGAAAGAGTAATAGAACCGGCCAACAGCCCCATCTGCGGATCAAGCCCTAACAAGCTAGCCTTAccaatgcc                                                                                               <  2:39863/149‑1 (MQ=255)                                                       ccACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATAccaatgcc                                                                                               >  6:267740/1‑148 (MQ=255)                                                        cACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCTGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATAccaatgccaa                                                                                             >  7:337134/1‑149 (MQ=255)                                                        cACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGa                                                                                                                               >  7:341203/1‑115 (MQ=255)                                                        cACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGa                                                                                                                               <  8:341203/115‑1 (MQ=255)                                                         aCTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATAccaatgccaat                                                                                            >  7:243348/1‑149 (MQ=255)                                                          cTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATAccaatgccaatg                                                                                           >  1:58291/1‑149 (MQ=255)                                                           ttCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCATACCGTGACCGCCGGAAAGAGTAATAGCACCTGCCACCAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACAAATGCCAATgg                                                                                          >  3:241769/1‑149 (MQ=255)                                                              cGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCa                                                                                        <  3:92167/148‑1 (MQ=255)                                                               gTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCAtt                                                                                      <  7:269917/149‑1 (MQ=255)                                                                tCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCAttt                                                                                     >  3:54463/1‑149 (MQ=255)                                                                 cGCATTCGTGAAGCCATAACGTTCAATCAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCAtttt                                                                                    <  5:219163/149‑1 (MQ=255)                                                                  gCATTGGTGAAGCCATAACGTTCGATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTg                                                                                   <  1:306065/149‑1 (MQ=255)                                                                  gCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCAtttt                                                                                    <  3:275161/148‑1 (MQ=255)                                                                   cATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGc                                                                                  <  3:162459/149‑1 (MQ=255)                                                                     ttGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCAt                                                                                <  8:243348/149‑1 (MQ=255)                                                                        gTGATGCCTTTACGTGCAAGGATCAATTTACTGCCCGCAGCGCCCGTACCGTGACCGCCGGATTGAGTAAAAGAACCGGCCAAGAGGCCCATCAGCGGCTCTAGGCCTAACAAGCTAGCCCTACCAATGCCAATGGCATTTTGCATCa                                                                              <  5:36172/148‑1 (MQ=255)                                                                        gTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCAc                                                                             >  6:267135/1‑149 (MQ=255)                                                                         tGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCAc                                                                             >  2:97078/1‑148 (MQ=255)                                                                          gAAGCGATAACGTTCAATGAACAAGTTACTCCACGCAGCGCCCGTACCGTGTCCGCCGGAAAGAGTATGAGAGCCGGCCAGCCGCCCCCTCAGCGGAGCGAGCCCGACCAAGCTAGCCCTACCAATGCCAAGGGCATTTTGctgcccca                                                                           >  6:281405/1‑142 (MQ=255)                                                                           aaGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCaa                                                                          <  8:242945/149‑1 (MQ=255)                                                                             gCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACa                                                                        <  5:63760/149‑1 (MQ=255)                                                                              ccATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCTGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAg                                                                       <  8:337134/149‑1 (MQ=255)                                                                                aTAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGAc                                                                     <  4:54463/149‑1 (MQ=255)                                                                                 tAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGAcc                                                                    <  3:267542/149‑1 (MQ=255)                                                                                           tGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACcac                                                           >  2:236135/1‑148 (MQ=255)                                                                                           tGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCAGTTTGCATCACCAACAGACCAACAACcac                                                           >  1:171731/1‑148 (MQ=255)                                                                                               cAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGa                                                                      <  8:54379/133‑1 (MQ=255)                                                                                               cAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGa                                                                      >  7:54379/1‑133 (MQ=255)                                                                                                aaTTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATAcc                                                                                                     >  8:48825/1‑101 (MQ=255)                                                                                                aaTTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATAcc                                                                                                     <  7:48825/101‑1 (MQ=255)                                                                                                aaTTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATc                                                       >  1:40147/1‑147 (MQ=255)                                                                                                aaTTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCaa                                                     <  3:175224/149‑1 (MQ=255)                                                                                                 aTTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATAccaatgc                                                                                                <  4:98840/105‑1 (MQ=255)                                                                                                 aTTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATAccaatgc                                                                                                >  3:98840/1‑105 (MQ=255)                                                                                                  tttACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAGCAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGCCCACCAACCACAATCaag                                                    >  8:134363/1‑148 (MQ=255)                                                                                                         cACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACcaacaa                                                               <  8:155285/130‑1 (MQ=255)                                                                                                         cACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACcaacaa                                                               >  7:155285/1‑130 (MQ=255)                                                                                                         cACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATg                                              <  1:97078/147‑1 (MQ=255)                                                                                                         cACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGc                                             >  7:206413/1‑148 (MQ=255)                                                                                                         cACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGc                                             >  3:85974/1‑148 (MQ=255)                                                                                                                 gcCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGCCAACCacac                                    >  3:147396/1‑149 (MQ=255)                                                                                                                     gTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGCCAACCACACGccc                                >  2:188399/1‑149 (MQ=255)                                                                                                                        ccGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGCCAACCACACGCCCAcc                             >  2:240031/1‑149 (MQ=255)                                                                                                                        ccGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGCCAACCACACGCCCAc                              <  2:40147/148‑1 (MQ=255)                                                                                                                               cgccgGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGCCAACCACACGCCCACCGGCACg                       >  7:331793/1‑148 (MQ=255)                                                                                                                                gccgGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACACGCTAGCCATACCAATGCCCATGGCATTTTGCATCCCCCACAGACCACCAACCACAAGAAAGCAGATGCCAACCACACGCCCACCGGCACGct                     >  6:325642/1‑148 (MQ=255)                                                                                                                                    gAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTACCAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGCCAACCACACGCCCACCGGCACGCAAACt                 <  4:125050/149‑1 (MQ=255)                                                                                                                                    gAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGCCAACCACACGCCCACCGGCACGCAAACt                 >  2:311416/1‑149 (MQ=255)                                                                                                                                     aaaGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGCCAACCACACGCCCACCGGCACGCAAACTg                <  3:321569/149‑1 (MQ=255)                                                                                                                                      aaGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACATGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGCCAACCACACGCCCACCGGCACGCAAACTgg               <  6:267795/149‑1 (MQ=255)                                                                                                                                         agTAATAGAACCGGCCCACAGCCCCATCAGCGGATCAAGCCCTACCAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGCCCAACACCCACACTCAAGAAGATGCCCGCCACACGCCCCCCGGCACGCACTCTGGCaa            >  5:339971/1‑149 (MQ=255)                                                                                                                                             aTAGAACCTGCCAACAGCCCCATCAGCGGATCAAGCCCTAACCAGATATCCATCCCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCAAAATCAAGAAGATGCCAACAACACTCCCCCCTGCACGCAAACTGGCAATGtt        <  6:29786/149‑1 (MQ=255)                                                                                                                                             aTAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGCCAACCACACGCCCACCGGCACGCAAACTGGCAATGtt        >  5:146181/1‑149 (MQ=255)                                                                                                                                                gAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGCCAACCACACGCCCACCGGCACGCAAACTGGCAATGTTGGc     <  1:236135/149‑1 (MQ=255)                                                                                                                                                   ccGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGCCAACCACACGCCCACCGGCACGCAAACTGGCAATGTTGGCGtt  >  1:24139/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCATCAGCGGATCAAGCCCTAACAAGCTAGCCATACCAATGCCAATGGCATTTTGCATCACCAACAGACCAACAACCACAATCAAGAAGATGCCAACCACACGCCCACCGGCACGCAAACTGGCAATGTTGGCGTT  >  NC_000913/3828131‑3828425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑