Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,968,349 T→A 5.6% V145D (GTC→GAC)  wecA → UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,968,3490TA 5.6% 220.2 / 3.6 72V145D (GTC→GAC) wecAUDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
Reads supporting (aligned to +/- strand):  ref base T (36/32);  new base A (2/2);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01

AGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCC  >  NC_000913/3968201‑3968497
                                                                                                                                                    |                                                                                                                                                    
aGCGTAAAAATCCGGGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGt                                                                                                                                                      <  4:116938/149‑1 (MQ=255)
    tAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTgg                                                                                                                                                  <  1:276936/149‑1 (MQ=255)
          tCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGcc                                                                                                                                            <  5:231445/149‑1 (MQ=255)
             gTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCAtt                                                                                                                                         <  7:229682/149‑1 (MQ=255)
                  accaTACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGc                                                                                                                                    >  2:216368/1‑149 (MQ=255)
                   ccaTACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCg                                                                                                                                   <  3:123824/149‑1 (MQ=255)
                   caaTACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGCTTATATCTTTGGCTCCTGGGAGATGGAGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCg                                                                                                                                   >  5:107589/3‑149 (MQ=255)
                    caTACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGTTTATATCTTTCGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGt                                                                                                                                  >  6:100092/1‑149 (MQ=255)
                     aTACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGtt                                                                                                                                 <  4:285702/149‑1 (MQ=255)
                      tccAGGCCGCTGTTGGCATTGCTATGATGGTGCTCGGCAAGCCTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGCTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTc                                                                                                                                <  6:283351/147‑1 (MQ=255)
                       aCAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTc                                                                                                                                >  1:51707/1‑148 (MQ=255)
                          ggCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACa                                                                                                                            <  6:53026/149‑1 (MQ=255)
                                  ttGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTg                                                                                                                      <  5:286829/147‑1 (MQ=255)
                                  ttGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGAt                                                                                                                    >  6:100325/1‑149 (MQ=255)
                                       aTTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCAt                                                                                                               >  8:90804/1‑149 (MQ=255)
                                           ttATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGAt                                                                                                           <  5:311000/149‑1 (MQ=255)
                                                 tgGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCGGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTg                                                                                                     >  8:470/1‑149 (MQ=255)
                                                 tgGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGtt                                                                                                                       >  5:291476/1‑131 (MQ=255)
                                                 tgGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGtt                                                                                                                       <  6:291476/131‑1 (MQ=255)
                                                 tgGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTg                                                                                                     >  3:301160/1‑149 (MQ=255)
                                                  ggTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTg                                                                                                     <  8:282065/148‑1 (MQ=255)
                                                        cGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAAGGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCg                                                                                              >  3:217815/1‑149 (MQ=255)
                                                           cAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGt                                                                                           >  4:304755/1‑149 (MQ=255)
                                                               cTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAACGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTc                                                                                       <  2:24606/149‑1 (MQ=255)
                                                               cTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCGCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTc                                                                                       <  7:205907/149‑1 (MQ=255)
                                                                tttATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTACTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTcc                                                                                      >  3:26488/1‑149 (MQ=255)
                                                                    tcgcAGTAGCCGGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGc                                                                                   <  7:64232/145‑1 (MQ=255)
                                                                      tcAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGACTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGtc                                                                                >  8:43931/1‑149 (MQ=255)
                                                                            gCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGttt                                                                          >  2:316602/1‑149 (MQ=255)
                                                                            gCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGtt                                                                           <  2:51707/148‑1 (MQ=255)
                                                                            gCCTGGGTTATATCTTTGGCTCCTGGGAGATGGCGCACGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCGGGGCGGCCATTACTGCGTTCATGATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGttt                                                                          <  3:218795/149‑1 (MQ=255)
                                                                               tGGGTTATATCTTTGGCTCCTGGGAGATGGTTCTCGGACCGTATGGTTACTTCCTGAAGCTATTTGCCGTCTTGGCGGCCCTTAATGCGTAAAACATGCTTGATGGCATTGATGGATTGCTTGGCGGGTTGTCCTGCGTCTAGTTTg                                                                         >  7:329578/1‑147 (MQ=255)
                                                                               tGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTg                                                                         >  4:202866/1‑147 (MQ=255)
                                                                                gggTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTgcag                                                                      >  2:325805/1‑149 (MQ=255)
                                                                                 ggTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTgcag                                                                      >  5:114264/1‑148 (MQ=255)
                                                                                      tatCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCg                                                                <  5:100325/149‑1 (MQ=255)
                                                                                      tatCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCg                                                                >  5:115002/1‑149 (MQ=255)
                                                                                        tCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTcc                                                                                      <  4:289748/125‑1 (MQ=255)
                                                                                        tCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTcc                                                                                      >  3:289748/1‑125 (MQ=255)
                                                                                              gCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGAtt                                                        <  6:114264/149‑1 (MQ=255)
                                                                                               ctacTGGGAGATGGTGCGCGGACCGTTTGGTTACTTCCTGACGATATTTGCCTTCTGGGCGGCCATTAATGCGTTCAAGATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCTGCTCGTTTGCAGCAATCGGTATGAttt                                                       >  8:309635/4‑149 (MQ=255)
                                                                                               cTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGAttt                                                       <  7:90804/149‑1 (MQ=255)
                                                                                                tCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGAtttt                                                      >  7:91441/1‑149 (MQ=255)
                                                                                                tCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGAtttt                                                      >  5:235344/1‑149 (MQ=255)
                                                                                                tCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGAtttt                                                      >  5:167440/1‑149 (MQ=255)
                                                                                                  cTGGGAGATGGTGCTCTGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTtgt                                                    <  5:13661/149‑1 (MQ=255)
                                                                                                  cTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTtg                                                     <  3:304755/148‑1 (MQ=255)
                                                                                                  cTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGACTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTa                                                             <  3:327303/140‑1 (MQ=255)
                                                                                                  cTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGACTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTa                                                             >  4:327303/1‑140 (MQ=255)
                                                                                                   tGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTtgtg                                                   >  2:2374/1‑149 (MQ=255)
                                                                                                    gggAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCACCATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTgg                                                  >  2:61561/1‑149 (MQ=255)
                                                                                                    gggAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGt                                                                            >  8:336865/1‑123 (MQ=255)
                                                                                                    gggAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGt                                                                            <  7:336865/123‑1 (MQ=255)
                                                                                                     ggAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGACTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTtgtg                                                   <  7:43931/147‑1 (MQ=255)
                                                                                                      gagaTGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGtt                                                <  1:325805/149‑1 (MQ=255)
                                                                                                       agttGGTGCTCGGACCGTTTGGTTACTTCCTGCCGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCATTCGGTATGATTTTGTGGTTc                                               <  6:107589/146‑1 (MQ=255)
                                                                                                           ggTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGAt                                                                                                           <  1:162548/85‑1 (MQ=255)
                                                                                                           ggTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGAt                                                                                                           >  4:5011/1‑85 (MQ=255)
                                                                                                           ggTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGAt                                                                                                           >  2:162548/1‑85 (MQ=255)
                                                                                                           ggTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTgcag                                                                      >  6:228722/1‑122 (MQ=255)
                                                                                                           ggTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTgcag                                                                      <  5:228722/122‑1 (MQ=255)
                                                                                                           ggTGCTCGGACAGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGAt                                                                                                           <  3:5011/85‑1 (MQ=255)
                                                                                                               cTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGc                                        >  2:277824/1‑148 (MQ=255)
                                                                                                                 cGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCaaa                                     >  1:24034/1‑149 (MQ=255)
                                                                                                                  ggACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCaaa                                     <  8:229114/148‑1 (MQ=255)
                                                                                                                         ttGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTc                             >  7:201567/1‑149 (MQ=255)
                                                                                                                            gTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTgca                                                                       >  6:335451/1‑104 (MQ=255)
                                                                                                                            gTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTgca                                                                       <  5:335451/104‑1 (MQ=255)
                                                                                                                                 ttCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTg                     >  3:280323/1‑149 (MQ=255)
                                                                                                                                   ccTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGt                   >  3:291785/1‑149 (MQ=255)
                                                                                                                                                gCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCgatga       <  1:316602/148‑1 (MQ=255)
                                                                                                                                                 ccGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCAGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATc     <  3:202866/149‑1 (MQ=255)
                                                                                                                                                  cGTCCGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATcg    >  3:159941/1‑149 (MQ=255)
                                                                                                                                                    tCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCGTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATcgcc  <  1:47537/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCC  >  NC_000913/3968201‑3968497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑