Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,188,953 G→A 100% E1202K (GAA→AAA)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,188,9530GA100.0% 346.8 / NA 99E1202K (GAA→AAA) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (51/48);  total (51/48)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAAC  >  NC_000913/4188808‑4189096
                                                                                                                                                 |                                                                                                                                               
ggCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAAc                                                                                                                                              <  4:103825/149‑1 (MQ=255)
    aTCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACgtgt                                                                                                                                          <  4:242895/149‑1 (MQ=255)
     tCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACgtg                                                                                                                                           >  8:136509/1‑147 (MQ=255)
     tCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTa                                                                                                                                         <  1:43762/149‑1 (MQ=255)
     tCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAAACAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTa                                                                                                                                         <  6:279521/149‑1 (MQ=255)
       cTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGGCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGCTGAATCCGAAATGGCGTCAGCTCAACGTGTTCTAAGGTAAACGTGTAGa                                                                                                                                       >  8:173446/1‑149 (MQ=255)
          gCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAAc                                                                                                                                     <  4:297764/148‑1 (MQ=255)
            tGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACgtg                                                                                                                                  >  7:231616/1‑149 (MQ=255)
            tGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACgtg                                                                                                                                  >  5:43916/1‑149 (MQ=255)
              aaaTCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACgtg                                                                                                                                  <  2:261311/147‑1 (MQ=255)
               aaTCCGCGGTATCGTTTCATTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCTGTAAACGGTATAGATCCTTACGAAGAGATGATTCCGAAATGGCGTCAGATCAACGTGTTCGAACGTAAACCTGTATAAActggt                                                                                                                                >  2:348528/1‑148 (MQ=255)
                aTCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGa                                                                                                                              >  5:253761/1‑149 (MQ=255)
                            gTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACgt                                                                                                                                   <  5:148809/132‑1 (MQ=255)
                            gTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACgt                                                                                                                                   >  6:148809/1‑132 (MQ=255)
                            gTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGa                                                                                                                  >  3:327322/1‑149 (MQ=255)
                                  ttCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTcc                                                                                                            >  5:315103/1‑149 (MQ=255)
                                  ttCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTcc                                                                                                            >  2:211527/1‑149 (MQ=255)
                                         aaGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACTAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAgc                                                                                                      <  8:231616/148‑1 (MQ=255)
                                         aaGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAgcg                                                                                                     >  6:146098/1‑149 (MQ=255)
                                          aGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTAAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTATTTTCCGACGGTCCGGAAgcgc                                                                                                    >  1:300836/1‑149 (MQ=255)
                                           gAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAAAGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGcgcc                                                                                                   >  1:297536/1‑149 (MQ=255)
                                              aCCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCa                                                                                                >  5:290534/1‑149 (MQ=255)
                                                        aaCGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATtct                                                                                       <  1:211527/148‑1 (MQ=255)
                                                         aCGTCGTCTGGTTATCACCCCTGTAGACGGTAGCGATCCGTACGTATAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCTCCGCACGACATtctgc                                                                                     <  4:327322/149‑1 (MQ=255)
                                                         aCGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATtctgc                                                                                     <  7:25377/149‑1 (MQ=255)
                                                              gtcTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATtctgcgtct                                                                                 <  8:8797/148‑1 (MQ=255)
                                                                   gTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGAc                                                                                                                             >  1:260610/1‑99 (MQ=255)
                                                                   gTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGAc                                                                                                                             <  2:260610/99‑1 (MQ=255)
                                                                   gTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCgtgg                                                                           >  7:209387/1‑149 (MQ=255)
                                                                   gTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAACGTAAACGTGTAGAACTAGGTGACCTAATTAACAACATAAAATAAGCGCACCACGACATACTGCGTCTGCgtgt                                                                           >  6:134768/1‑148 (MQ=255)
                                                                       tCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGtt                                                                       <  6:43916/149‑1 (MQ=255)
                                                                         aCCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACgtg                                                                                                                                           <  5:6350/79‑1 (MQ=255)
                                                                         aCCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACgtg                                                                                                                                           >  6:6350/1‑79 (MQ=255)
                                                                         aCCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATtctgc                                                                                     >  6:12338/1‑133 (MQ=255)
                                                                         aCCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATtctgc                                                                                     <  5:12338/133‑1 (MQ=255)
                                                                         aCCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCa                                                                     <  6:42481/149‑1 (MQ=255)
                                                                         aCCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCa                                                                     >  2:196978/1‑149 (MQ=255)
                                                                          ccccGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCAc                                                                                               <  4:173263/122‑1 (MQ=255)
                                                                          ccccGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCAc                                                                                               >  3:173263/1‑122 (MQ=255)
                                                                          ccccGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCgtggt                                                                          <  1:313991/143‑1 (MQ=255)
                                                                          ccccGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCgtggt                                                                          >  2:313991/1‑143 (MQ=255)
                                                                          ccccGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCAt                                                                    <  6:315103/149‑1 (MQ=255)
                                                                          ccccGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCAt                                                                    >  5:34057/1‑149 (MQ=255)
                                                                           cccGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGTTCGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATg                                                                   <  2:260350/149‑1 (MQ=255)
                                                                           cccGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACTAGGTGAACTAATTAACAAAAAAAAAAAAACGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATg                                                                   >  2:12725/1‑149 (MQ=255)
                                                                           cccGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATtct                                                                                       >  8:96915/1‑129 (MQ=255)
                                                                           cccGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATtct                                                                                       <  7:96915/129‑1 (MQ=255)
                                                                            ccGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGc                                                                  >  7:118197/1‑149 (MQ=255)
                                                                             cGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCt                                                                 <  6:290534/149‑1 (MQ=255)
                                                                                  gACGGTAGCGATCCTTACGAAGATATGATTCCGAAATGGCGTCAGCTGAACGTGTTCGAAGGTACAGGGGTAGAACGTTGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTa                                                             <  1:348528/148‑1 (MQ=255)
                                                                                   aCGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACt                                                           <  7:136509/149‑1 (MQ=255)
                                                                                          cGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGAGGTAATTTACGACGATACGGAAGCGCCGCACGACAATATGCTTCTGCGTGGTGTTGATGCTGTTACTCGTTAc                                                     >  2:325956/1‑148 (MQ=255)
                                                                                          cGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCAc                                                                                               <  7:157094/106‑1 (MQ=255)
                                                                                          cGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCAc                                                                                               >  8:157094/1‑106 (MQ=255)
                                                                                          cGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACa                                                    >  2:321134/1‑149 (MQ=255)
                                                                                           gATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCATACGTTAAGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTAGTTACAt                                                   >  8:169913/1‑149 (MQ=255)
                                                                                            aTCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATc                                                  >  2:203481/1‑149 (MQ=255)
                                                                                            aTCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATc                                                  >  7:55547/1‑149 (MQ=255)
                                                                                               cGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGtt                                               <  8:145278/149‑1 (MQ=255)
                                                                                                gTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCTCCGCAAGACATTCTGAGTGTGCGTTGTGTTCATGCTGtt                                                              >  8:239556/1‑133 (MQ=255)
                                                                                                gTACGAAGAGATGATACCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTTACGTAATTTCCGACGGTCCGTAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGtt                                                              <  7:239556/133‑1 (MQ=255)
                                                                                                   cGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCTCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAAc                                            <  4:161809/148‑1 (MQ=255)
                                                                                                   cGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAAc                                            >  8:251680/1‑148 (MQ=255)
                                                                                                    gAAGAGATGATTCCGAAATGGCGTCAGCTCGACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACg                                           >  7:197548/1‑148 (MQ=255)
                                                                                                     aaGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGaa                                         <  8:209387/149‑1 (MQ=255)
                                                                                                      agagATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAg                                        <  6:253761/149‑1 (MQ=255)
                                                                                                      agagATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAg                                        <  6:34057/149‑1 (MQ=255)
                                                                                                        agaTGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGAc                                                                                            <  8:12380/95‑1 (MQ=255)
                                                                                                         gatgatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTa                                      >  8:210829/1‑148 (MQ=255)
                                                                                                          atgatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACa                                    <  5:146098/149‑1 (MQ=255)
                                                                                                          atgatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACa                                    <  1:296240/149‑1 (MQ=255)
                                                                                                           tgatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAg                                   <  7:225691/149‑1 (MQ=255)
                                                                                                               tCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCt                                                                 <  8:54770/115‑1 (MQ=255)
                                                                                                               tCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCt                                                                 >  7:54770/1‑115 (MQ=255)
                                                                                                               tCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACg                               >  8:248085/1‑149 (MQ=255)
                                                                                                               tCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACg                               >  5:177813/1‑149 (MQ=255)
                                                                                                                ccGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACg                               <  7:8628/148‑1 (MQ=255)
                                                                                                                 cGAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGta                             <  7:210829/149‑1 (MQ=255)
                                                                                                                  gAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGtat                            <  7:251680/149‑1 (MQ=255)
                                                                                                                  gAAATGGCGTCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGta                             >  5:315214/1‑148 (MQ=255)
                                                                                                                        gCGTCAGCGCAACGTGTTCGAAGGTAAACGTGTAGATCGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTTGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGt                       <  2:297536/148‑1 (MQ=255)
                                                                                                                           tCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAAc                                            >  3:309023/1‑124 (MQ=255)
                                                                                                                           tCAGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAAc                                            <  4:309023/124‑1 (MQ=255)
                                                                                                                             aGCTCTACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTACGACGGTCCGGAAGCGCCGCACGACATTCTGAGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCa                  <  1:325956/148‑1 (MQ=255)
                                                                                                                             aGCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCa                  <  1:321134/148‑1 (MQ=255)
                                                                                                                              gCTCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAgg                >  2:36353/1‑149 (MQ=255)
                                                                                                                                tCAACGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGc              >  1:306450/1‑149 (MQ=255)
                                                                                                                                  aaCGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGt            >  4:51759/1‑149 (MQ=255)
                                                                                                                                  aaCGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGt            >  5:71634/1‑149 (MQ=255)
                                                                                                                                  aaCGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGGGGTGGTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGt            <  7:173446/149‑1 (MQ=255)
                                                                                                                                   aCGTGTTCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGt            >  7:147469/1‑148 (MQ=255)
                                                                                                                                        ttCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGa       <  1:196978/148‑1 (MQ=255)
                                                                                                                                        ttCGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGAt      >  5:256280/1‑149 (MQ=255)
                                                                                                                                          cGAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGAt      >  8:329333/1‑147 (MQ=255)
                                                                                                                                           gAAGGTAAACGTGTAGAACGTTGTGACGTAATTTCCGACGGTCCGGAAGCGCCGAACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTaa   <  5:134768/149‑1 (MQ=255)
                                                                                                                                           gAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTaa   >  3:96245/1‑149 (MQ=255)
                                                                                                                                           gAAGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTaa   <  2:300836/149‑1 (MQ=255)
                                                                                                                                            aaGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAAc  >  7:70584/1‑149 (MQ=255)
                                                                                                                                             aGGTAAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAAc  >  8:174758/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAAC  >  NC_000913/4188808‑4189096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: