Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,197,519 C→T 6.4% A206V (GCG→GTG)  nudC → NADH pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,197,5190CT 6.4% 240.9 / 3.8 77A206V (GCG→GTG) nudCNADH pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (39/33);  new base T (3/2);  total (42/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCA  >  NC_000913/4197372‑4197664
                                                                                                                                                   |                                                                                                                                                  
aaaactgATTCGTCGAAGTGGGCGAAACACTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCg                                                                                                                                                   <  3:204968/143‑1 (MQ=255)
   ccGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGaa                                                                                                                                                <  4:231836/149‑1 (MQ=255)
      gATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAatat                                                                                                                                             <  3:295131/149‑1 (MQ=255)
           gtcgAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGc                                                                                                                                                    <  6:114603/137‑1 (MQ=255)
           gtcgAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGc                                                                                                                                                    >  5:114603/1‑137 (MQ=255)
              gAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAACCTTGCGGTACGTGACTTCTCAGCCGTGGCCGTATCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACCgcgg                                                                                                                                     >  1:123311/1‑149 (MQ=255)
              gAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGTCGGAATATGACAgcgg                                                                                                                                     >  6:63749/1‑149 (MQ=255)
               aaGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAgcggc                                                                                                                                    >  8:5082/1‑149 (MQ=255)
                aGTGGGCGAAACCCTCGAGCAGGCAGGCGCGCGGGAAGTGATGGAAGAGAGCGTAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAgcggcg                                                                                                                                   <  5:19738/149‑1 (MQ=255)
                         aaCCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑a                                                                                                                         <  1:181710/149‑1 (MQ=255)
                            ccTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATc                                                                                                                       >  8:227662/1‑148 (MQ=255)
                            ccTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATc                                                                                                                       >  4:298180/1‑148 (MQ=255)
                            ccTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATc                                                                                                                       >  3:114448/1‑148 (MQ=255)
                              tCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGAc                                                                                                                    >  2:223500/1‑149 (MQ=255)
                                gAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGAc                                                                                                                    <  7:227662/147‑1 (MQ=255)
                                  gcaggcagTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCTGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCg                                                                                                                 >  6:282312/1‑148 (MQ=255)
                                       cagTCGCGCGGGAATTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTAAGTGACTTATCAGCCGTGGCAGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGTAATATGACACAGGCGACATCGTGAAACGA‑CCGAAAGaa                                                                                                           >  6:218288/1‑149 (MQ=255)
                                             cgcgGGAAGTGATGGAAGCGAGCGGAATTAACGTTCAAAACTTCCGTTACGTGACATCGCAGCCGTGGCCGTCTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTc                                                                                                     <  4:190712/149‑1 (MQ=255)
                                                  gAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGc                                                                                                <  8:151553/149‑1 (MQ=255)
                                                    aGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCg                                                                                               >  4:28438/1‑148 (MQ=255)
                                                      tGATGGAAGAGAGCGGAATTACACTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGCTTCCTCAGTCTTTAATGACCGCGTTTATCGCGGAATATGACAGCGCCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAAc                                                                                            <  5:121249/149‑1 (MQ=255)
                                                      tGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAAc                                                                                            <  7:218896/149‑1 (MQ=255)
                                                        aTGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGCCATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAAcgg                                                                                          >  4:92533/1‑147 (MQ=255)
                                                        aTGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAg                                                                                                  <  6:326450/141‑1 (MQ=255)
                                                        aTGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAg                                                                                                  >  5:326450/1‑141 (MQ=255)
                                                        aTGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTg                                                                                          <  7:5082/149‑1 (MQ=255)
                                                        aTGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGAGAACTg                                                                                          >  6:34599/1‑149 (MQ=255)
                                                                 agCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGt                                                                                                                            <  7:12450/107‑1 (MQ=255)
                                                                 agCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGt                                                                                                                            >  8:12450/1‑107 (MQ=255)
                                                                 agCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTa                                                                                 >  6:288622/1‑149 (MQ=255)
                                                                  gCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTAt                                                                                <  3:298180/149‑1 (MQ=255)
                                                                  gCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTAt                                                                                <  1:223500/149‑1 (MQ=255)
                                                                   cGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTAt                                                                                >  6:301424/1‑148 (MQ=255)
                                                                    ggAATTAAAGTTAAAAACTTGCGTTACGGGACTTCTCAGCCGTGGCAGTTTCCTCAGTCTTTAATGACCGCGGTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGa                                                                              <  2:271574/149‑1 (MQ=255)
                                                                     gAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGTGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGAc                                                                             >  3:218119/1‑149 (MQ=255)
                                                                       aTTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGa                                                                           >  1:106387/1‑149 (MQ=255)
                                                                          aaaGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAgcggcg                                                                                                                                   >  3:106222/1‑91 (MQ=255)
                                                                          aaaGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAgcggcg                                                                                                                                   <  4:106222/91‑1 (MQ=255)
                                                                           aaGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTg                                                                       >  4:307663/1‑149 (MQ=255)
                                                                           aaGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGGCTTTAATGACCGCGTTTATGGCGGAATAGGACAGCGGCGCCATCGTG‑ATCGACCCGATAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTg                                                                       >  2:87115/1‑149 (MQ=255)
                                                                            aGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGc                                                                      >  4:287971/1‑149 (MQ=255)
                                                                            aGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGc                                                                      <  5:301424/149‑1 (MQ=255)
                                                                              ttAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCg                                                                    >  5:135195/1‑149 (MQ=255)
                                                                               tAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGTGGAATATGACAGCGGCGACATCGTG‑ATc                                                                                                                       <  1:227628/97‑1 (MQ=255)
                                                                               tAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGTGGAATATGACAGCGGCGACATCGTG‑ATc                                                                                                                       >  2:227628/1‑97 (MQ=255)
                                                                               tAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGTGGAATATGACAGCGGCGACATCGTG‑ATc                                                                                                                       >  2:232460/1‑97 (MQ=255)
                                                                               tAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGTGGAATATGACAGCGGCGACATCGTG‑ATc                                                                                                                       <  1:232460/97‑1 (MQ=255)
                                                                                   aaCTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACt                                                               <  2:106387/149‑1 (MQ=255)
                                                                                   aaCTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACt                                                               >  3:54294/1‑149 (MQ=255)
                                                                                     cTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTcc                                                             <  3:287971/149‑1 (MQ=255)
                                                                                                 gACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCa                                                 >  8:170629/1‑149 (MQ=255)
                                                                                                 gACTTCTCAGCCGTGGCCGTTTCGTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGATCGAGGCGAGATGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCa                                                 >  1:88404/1‑149 (MQ=255)
                                                                                                      ctcAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGCCAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTa                                            >  6:14498/1‑149 (MQ=255)
                                                                                                      cgatctcGTGGCCGTTTCCTCAGTCTATAATGACCGCGTTTATGGCGGAATATGACCGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCCATGACGATTTGCCGTTACTCCCCCCGCCCGGCACCg                                              <  6:154103/141‑1 (MQ=255)
                                                                                                       tcAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAg                                           >  7:69427/1‑149 (MQ=255)
                                                                                                         aGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTa                                            <  4:114448/146‑1 (MQ=255)
                                                                                                                   gTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGa                               >  4:195809/1‑149 (MQ=255)
                                                                                                                   gTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGa                               <  3:28438/149‑1 (MQ=255)
                                                                                                                    tttCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGAt                              <  3:307663/149‑1 (MQ=255)
                                                                                                                     ttCCTCCGTCTTTATTGACAGCGTTTATGGCGGAATATGACAGCGGCGACAGCGTG‑TGCGACCCGAAAGAATTGCTCGAGGCGAACTGGTAGCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGa                               <  5:218288/147‑1 (MQ=255)
                                                                                                                         tCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTcc                                                             <  6:62199/113‑1 (MQ=255)
                                                                                                                         tCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTcc                                                             >  5:62199/1‑113 (MQ=255)
                                                                                                                           aGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATagaaga                        >  4:91322/1‑148 (MQ=255)
                                                                                                                             tCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGAACCGAAAGAATTGCTCGAGGCGAACTGGTATCTCTATGACGATTTGCCGTTACTCACGCCGCCCGGCAACGTAGCGCGCCGTCTGATAGAAGATAc                     >  5:58367/1‑149 (MQ=255)
                                                                                                                               tttAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACgg                   >  7:154178/1‑149 (MQ=255)
                                                                                                                               tttAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACgg                   >  4:125724/1‑149 (MQ=255)
                                                                                                                               tttAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACg                    >  2:194610/1‑148 (MQ=255)
                                                                                                                                 tAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACg                    <  1:194610/146‑1 (MQ=255)
                                                                                                                                  aaTGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTg                 <  3:91322/148‑1 (MQ=255)
                                                                                                                                   aTGACCGCGTTTCTGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGc               <  4:229300/149‑1 (MQ=255)
                                                                                                                                   aTGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGc               >  7:3145/1‑149 (MQ=255)
                                                                                                                                      aCCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCTACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGAt            <  4:139334/149‑1 (MQ=255)
                                                                                                                                      aCCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGATTTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGAt            <  2:177549/149‑1 (MQ=255)
                                                                                                                                       ccGCGTTTATGGCGGAATATGACAGAGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGAGTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATATAAGATACGGTGGCg              >  3:35522/1‑146 (MQ=255)
                                                                                                                                        cgcgTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGAtgt          >  6:339112/1‑149 (MQ=255)
                                                                                                                                                tGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTgg                >  6:39348/1‑135 (MQ=255)
                                                                                                                                                tGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTgg                <  5:39348/135‑1 (MQ=255)
                                                                                                                                                tGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCa  <  5:288622/149‑1 (MQ=255)
                                                                                                                                                  gCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCa  >  5:302461/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTG‑ATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCA  >  NC_000913/4197372‑4197664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑