Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,167,309 C→G 5.6% H409D (CAT→GAT)  ndh → respiratory NADH dehydrogenase 2/cupric reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,167,3090CG 5.6% 240.3 / 5.5 72H409D (CAT→GAT) ndhrespiratory NADH dehydrogenase 2/cupric reductase
Reads supporting (aligned to +/- strand):  ref base C (34/34);  new base G (2/2);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

AAAGATCATGGTTCGCTGGTATCGCTGTCGAACTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAGCGCGATCGGCAGC  >  NC_000913/1167162‑1167456
                                                                                                                                                   |                                                                                                                                                   
aaaGATCATGGTTCGCTGGTATCGCTGTCGAACTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCa                                                                                                                                                    >  1:23934/1‑149 (MQ=255)
          gTTCGCTGGTATCGCTGGCGAACTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAGTGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACtt                                                                                                                                           <  6:93585/148‑1 (MQ=255)
              gCTGGTATCGCTGTCGAACTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTaaaa                                                                                                                                      >  4:46635/1‑149 (MQ=255)
               cTGGTATCGCTGTCGAACTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTaaaaa                                                                                                                                     >  5:208483/1‑148 (MQ=255)
               cTGGTATCGCTCTCGAACTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAAc                                                                                                                                     >  8:327372/1‑149 (MQ=255)
                tGGTATCGCTGTCGAACTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAAcc                                                                                                                                    >  8:149584/1‑149 (MQ=255)
                       gCTGTCGAACTTCTCCACCGTCTTTATCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTaa                                                                                                                             <  3:99391/149‑1 (MQ=255)
                           tCGAACTTCTCCACCGTCGGTAGCCTGATTGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAatga                                                                                                                          <  1:77952/148‑1 (MQ=255)
                           tCGAACTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGGGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAatgat                                                                                                                         <  5:204309/149‑1 (MQ=255)
                           tCGAACTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTTTACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAatgat                                                                                                                         >  3:123750/1‑149 (MQ=255)
                           tCGAACTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAatgat                                                                                                                         >  6:52217/1‑149 (MQ=255)
                           tCGAACTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGAATGCGCTGCATGGTTACTTTAAAACCGGATTAatga                                                                                                                          <  6:108555/148‑1 (MQ=255)
                              aaCTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAat                                                                                                                            <  4:227113/143‑1 (MQ=255)
                              aaCTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAat                                                                                                                            >  3:227113/1‑143 (MQ=255)
                              aaCTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGATATACCTAATGCATCAGATTGCTCTGCATGGTTACTTTAAAACCTGATTAATGATGCt                                                                                                                      >  6:232400/1‑149 (MQ=255)
                                cTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCtgg                                                                                                                    >  1:2321/1‑149 (MQ=255)
                                   ctcCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCtggt                                                                                                                   <  5:52217/147‑1 (MQ=255)
                                      cACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGa                                                                                                              <  3:46635/149‑1 (MQ=255)
                                      cACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGa                                                                                                              <  1:300882/149‑1 (MQ=255)
                                      cACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGa                                                                                                              <  4:182546/149‑1 (MQ=255)
                                       aCCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAg                                                                                                             >  1:295130/1‑149 (MQ=255)
                                              gTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATgg                                                                                                                                                 >  6:166162/1‑106 (MQ=255)
                                              gTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATgg                                                                                                                                                 <  5:166162/106‑1 (MQ=255)
                                              gTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAAc                                                                                                      >  2:334838/1‑149 (MQ=255)
                                               tAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAAcc                                                                                                     <  7:327372/149‑1 (MQ=255)
                                                   cTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGt                                                                                                 >  3:227559/1‑149 (MQ=255)
                                                   cTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGt                                                                                                 >  2:127498/1‑149 (MQ=255)
                                                     gATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCtggt                                                                                                                   <  8:105851/129‑1 (MQ=255)
                                                     gATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCtggt                                                                                                                   >  7:105851/1‑129 (MQ=255)
                                                      aTGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTAt                                                                                              <  7:149584/149‑1 (MQ=255)
                                                       tGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTAtc                                                                                             >  8:8399/1‑149 (MQ=255)
                                                         ggTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTAtcc                                                                                            <  2:2321/148‑1 (MQ=255)
                                                          gTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTAtccgt                                                                                          >  6:313583/1‑149 (MQ=255)
                                                             aCCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTAtccgtcc                                                                                        <  2:23934/148‑1 (MQ=255)
                                                                  aCGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGttt                                                                                  <  6:239057/149‑1 (MQ=255)
                                                                    gcgcgGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGa                                                                                <  2:295130/149‑1 (MQ=255)
                                                                     cgcgGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATCCCGAATGCATCAGATAGCGCTGCATGGTTACTTTAAACCCGGATTAAAGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGaa                                                                               <  6:210195/149‑1 (MQ=255)
                                                                     cgcgGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACcgcg                                                                                                  <  4:107752/130‑1 (MQ=255)
                                                                     cgcgGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACcgcg                                                                                                  >  3:107752/1‑130 (MQ=255)
                                                                     cgcgGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGaa                                                                               <  7:44573/149‑1 (MQ=255)
                                                                             aaTGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCCTCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGGGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCAt                                                                        <  3:255632/148‑1 (MQ=255)
                                                                               tgatgaTTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTaa                                                                     >  6:29151/1‑149 (MQ=255)
                                                                                gatgagTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAAt                                                                    >  5:57165/1‑149 (MQ=255)
                                                                                    atTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGAc                                                                >  2:48950/1‑149 (MQ=255)
                                                                                    atTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACAGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCAGCGTTTGAAGTTGCATTAATCGAc                                                                >  7:246509/1‑149 (MQ=255)
                                                                                    atTGAACGAAGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACAGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCAGCGTTTGAAGTTGCATTAATCGAc                                                                >  6:42139/1‑149 (MQ=255)
                                                                                     ttGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTTTACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGAc                                                                <  4:123750/148‑1 (MQ=255)
                                                                                        aaGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGGGGGGAGTATTAACCGCTTTAGCCGTCCGCGTTTGAAGTTGCTTTAAGCGACGtcc                                                            >  6:100622/1‑147 (MQ=255)
                                                                                                    cgcgcTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGc                                                                          <  6:213794/123‑1 (MQ=255)
                                                                                                    cgcgcTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGc                                                                          >  5:213794/1‑123 (MQ=255)
                                                                                                     gcgcTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGAtgtg                                               <  8:289334/149‑1 (MQ=255)
                                                                                                      cgcTTTGTATATATCTCGCTATACCGACTGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTgg                                              <  5:232400/149‑1 (MQ=255)
                                                                                                       gcTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGc                                             <  1:127498/149‑1 (MQ=255)
                                                                                                       gcTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCGGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGc                                             <  6:268421/149‑1 (MQ=255)
                                                                                                                atatCTCGCTATACCGAATGCATCAGATTGCGCTGGATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGAtgtg                                               <  8:41045/138‑1 (MQ=255)
                                                                                                                atatCTCGCTATACCGAATGCATCAGATTGCGCTGGATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGAtgtg                                               >  7:41045/1‑138 (MQ=255)
                                                                                                                atatCTCGCTATACCGAATGCATCAGATTGCGCTGGATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGAtgtg                                               >  7:46381/1‑138 (MQ=255)
                                                                                                                atatCTCGCTATACCGAATGCATCAGATTGCGCTGGATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGAtgtg                                               <  8:46381/138‑1 (MQ=255)
                                                                                                                atatCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGcc                                    >  8:124362/1‑149 (MQ=255)
                                                                                                                   tctcGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGAc                                                                >  3:109647/1‑118 (MQ=255)
                                                                                                                   tctcGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGAc                                                                <  4:109647/118‑1 (MQ=255)
                                                                                                                         tataCCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGcc                           >  1:251646/1‑149 (MQ=255)
                                                                                                                           taCCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTg                                                        <  7:262738/118‑1 (MQ=255)
                                                                                                                           taCCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTg                                                        >  8:262738/1‑118 (MQ=255)
                                                                                                                              cGATTGCATCAGATTGCGCTGCATGGGTACTTAAAAAACGGATTCATGATGCTGGGGGGGAGTATTAACCGCGTTAACCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGGGGCATAAACGCCTAATCCGCCCTTGa                      <  2:106986/149‑1 (MQ=255)
                                                                                                                                aaTGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAgg                    <  7:8399/149‑1 (MQ=255)
                                                                                                                                    cATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACa                <  5:29151/149‑1 (MQ=255)
                                                                                                                                      tCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAgc              >  2:328884/1‑149 (MQ=255)
                                                                                                                                            tGCGCTGCATGGTTACTTTAAAACCGGAGTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAGCGCGATc        >  3:290534/1‑149 (MQ=255)
                                                                                                                                                cTGCCTGGTTACTTTAAAACCGGATTAATGATGCGGGGGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTACTCGACGTACACTGGCGGATGTGGCATAAACGCCGCATCCGCCCTTGAGGAACAGCGCGATCGGCa    <  4:227559/149‑1 (MQ=255)
                                                                                                                                                 tGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAGCGCGATCGGCAg   >  8:335202/1‑149 (MQ=255)
                                                                                                                                                  gCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAGCGCGATCGGCAGc  >  7:117551/1‑149 (MQ=255)
                                                                                                                                                   cATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAGCGCGATCGGCAGc  <  8:117551/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AAAGATCATGGTTCGCTGGTATCGCTGTCGAACTTCTCCACCGTCGGTAGCCTGATGGGTAACCTGACGCGCGGCTCAATGATGATTGAAGGACGAATTGCGCGCTTTGTATATATCTCGCTATACCGAATGCATCAGATTGCGCTGCATGGTTACTTTAAAACCGGATTAATGATGCTGGTGGGGAGTATTAACCGCGTTATCCGTCCGCGTTTGAAGTTGCATTAATCGACGTACACTGGCGGATGTGGCATAAACGCCTCATCCGCCCTTGAGGAACAGCGCGATCGGCAGC  >  NC_000913/1167162‑1167456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: