Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,566,420 G→C 5.4% L241V (CTG→GTG)  dosC ← diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,566,4200GC 5.4% 251.8 / 5.5 74L241V (CTG→GTG) dosCdiguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator
Reads supporting (aligned to +/- strand):  ref base G (36/34);  new base C (2/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

TTCTTCAAACAATTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTTCCCA  >  NC_000913/1566274‑1566567
                                                                                                                                                  |                                                                                                                                                   
ttcttcAAACAATTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGAc                                                                                                                                                   >  3:225334/1‑149 (MQ=255)
ttcttcAAACAATTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTATGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGAc                                                                                                                                                   >  1:197251/1‑149 (MQ=255)
     cAAACAATTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCTTCGAAATCCTGAATCAGACgg                                                                                                                                                 >  6:244023/1‑146 (MQ=255)
     cAAACAATTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGga                                                                                                                                               <  4:153215/148‑1 (MQ=255)
      aaaCAATTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACgg                                                                                                                                                 >  2:185315/1‑145 (MQ=255)
      aaaCAATTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACgg                                                                                                                                                 >  2:15011/1‑145 (MQ=255)
      aaaCAATTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGgaga                                                                                                                                             <  2:196930/149‑1 (MQ=255)
      aaaCAATTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGgag                                                                                                                                              <  4:212593/148‑1 (MQ=255)
      aaaCAATTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGAAgg                                                                                                                                                 >  4:249776/1‑145 (MQ=255)
       aaCAATTCACGCAGCAAGGTAATAATTTGCGATAAGGTATTTCTTATCTGTAATAAAAATTTCACCAGCAGACTTCTGTTATTCAAATTTATGGTGTTACGAATGGTTTGATTGAAAATACCGTCGAAATAATGAATAAGACGGgaga                                                                                                                                             >  7:34945/1‑148 (MQ=255)
        aCAATTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGata                                                                                                                                           >  7:150210/1‑149 (MQ=255)
         cAATTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGatat                                                                                                                                          <  4:220231/149‑1 (MQ=255)
          aaTTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATg                                                                                                                                         <  1:306270/149‑1 (MQ=255)
          aaTTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATg                                                                                                                                         <  8:247487/149‑1 (MQ=255)
             tCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGcc                                                                                                                                      >  7:77718/1‑149 (MQ=255)
               aCGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTa                                                                                                                                    >  5:298887/1‑149 (MQ=255)
                      aaGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGc                                                                                                                             <  8:6607/149‑1 (MQ=255)
                       aGGTAATAATTAGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCa                                                                                                                            <  2:110579/149‑1 (MQ=255)
                        ggTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGgaga                                                                                                                                             <  3:124781/131‑1 (MQ=255)
                        ggTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGgaga                                                                                                                                             >  4:124781/1‑131 (MQ=255)
                        ggTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCaa                                                                                                                           >  3:317994/1‑149 (MQ=255)
                           aataatTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTTGTGTTACGCATGGTTTGATTGAAAATACAGTCGAATTCCTGAATCAGACGGGAGATATGGCCTAGTTCGGGAATAc                                                                                                                        >  5:87041/1‑149 (MQ=255)
                            atattTTGAGATTCGGTATTTCTTATCTGTAATAAAAATATCACCCGAAGACTTCTGTTATTCAAATTTGTGGCTTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATAc                                                                                                                        <  8:34945/148‑1 (MQ=255)
                               atTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACt                                                                                                                    <  6:298887/149‑1 (MQ=255)
                                     gATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAaata                                                                                                              <  8:174733/149‑1 (MQ=255)
                                                    aTCTGTAATAAAAATTTCACCCGCAGACTTCTGATATTCAACTCTCTGGTGATACGCATGGTTTGATGGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTa                                                                                                 <  6:163147/147‑1 (MQ=255)
                                                     tCTGTAATAAAAATTTCACCCGCAGACTGCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTGCGGCAATACCACTAAAATAATGTCGACCTTTATgg                                                                                              >  4:254342/1‑149 (MQ=255)
                                                       tGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGt                                                                                             >  6:243785/1‑148 (MQ=255)
                                                             aaaaaTTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACca                                                                                      >  7:57964/1‑149 (MQ=255)
                                                             aaaaaTTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCACACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACca                                                                                      >  8:55777/1‑149 (MQ=255)
                                                             aaaaaTTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCACACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACca                                                                                      >  8:61368/1‑149 (MQ=255)
                                                              taattttCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCAGGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGTCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACcac                                                                                     <  6:41195/145‑1 (MQ=255)
                                                              aaaaTTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACca                                                                                      >  4:155129/1‑148 (MQ=255)
                                                                      aCCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCaa                                                                              >  6:189744/1‑148 (MQ=255)
                                                                            aGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGCTTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTGCGGCAATACCACTACAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGc                                                                       <  8:251413/149‑1 (MQ=255)
                                                                              aCTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCt                                                                     <  8:150210/149‑1 (MQ=255)
                                                                              aCTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCt                                                                     >  5:100130/1‑149 (MQ=255)
                                                                              aCTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCt                                                                     <  4:317994/149‑1 (MQ=255)
                                                                                  cTGTTATTCAAATTTCTGGTGTTACGCATGGTGTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGc                                                                  <  1:300846/148‑1 (MQ=255)
                                                                                   tGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCt                                                                 >  2:351067/1‑148 (MQ=255)
                                                                                     ttATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCtt                                                                <  7:36193/147‑1 (MQ=255)
                                                                                       aTTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTaaaa                                                                                                                >  2:188216/1‑97 (MQ=255)
                                                                                       aTTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTaaaa                                                                                                                <  1:188216/97‑1 (MQ=255)
                                                                                       aTTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCACACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTaaa                                                             <  7:61368/148‑1 (MQ=255)
                                                                                       aTTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCACACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTaaa                                                             <  7:55777/148‑1 (MQ=255)
                                                                                        ttCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAgg                                                           >  7:203032/1‑149 (MQ=255)
                                                                                         tCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCTTCGAAATCCTTAATCAGACGGGAGATATGGCCTACTTCGTCAATACCACTAAAATAATGTCGACCTTAATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAgg                                                           >  3:43405/1‑148 (MQ=255)
                                                                                          cAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGc                                                          <  5:258814/148‑1 (MQ=255)
                                                                                             aTTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAac                                                      >  1:268825/1‑149 (MQ=255)
                                                                                                   ggTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGc                                                >  3:28745/1‑149 (MQ=255)
                                                                                                     tgtTACGCATGGTTTGATTGAAAATACCGTCGACATCCTGATTCTGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACATGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCt                                              <  2:293119/149‑1 (MQ=255)
                                                                                                           gCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTTAACCACAGGCCAAAATAAGCCTGGCTTAAAGGCAAACTACTGCCGAAAt                                          >  6:284883/1‑147 (MQ=255)
                                                                                                           gCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAAt                                          >  5:1136/1‑147 (MQ=255)
                                                                                                             aTGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGa                                      <  8:77718/149‑1 (MQ=255)
                                                                                                             aTGGGTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGa                                      <  4:45558/149‑1 (MQ=255)
                                                                                                               ggTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAAt                                    >  7:256224/1‑149 (MQ=255)
                                                                                                                gTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAactac                                                   >  8:53692/1‑133 (MQ=255)
                                                                                                                gTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAactac                                                   <  7:53692/133‑1 (MQ=255)
                                                                                                                gTTTGATTGAAAATACCGTCGAAATCCTGAAGCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATc                                   <  3:249776/149‑1 (MQ=255)
                                                                                                                     aatgaAAAAACCTTCGAAATCCTGAATAAGACAGGAGAAATTGCCTACATCGGCAAAACAACTAAAATAATGTCGACCTTTATAGTTAAACAACACGCCAAAAAAAGCCAAACTTAAACGCAAACTACTCCCTAAAAAACAATAcagca                              >  4:73983/3‑149 (MQ=255)
                                                                                                                      ttgaAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCcaacag                             >  1:133950/1‑149 (MQ=255)
                                                                                                                      ttgaAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACAACTAAAATAATGTCGACCTTTATGCTTAAACCACAGGCCAAAATCAGCCTGGATTAAAGGCAAACTAATCCCTAAATCAGAATCcagaaa                             >  4:254057/1‑146 (MQ=255)
                                                                                                                         aaaaTACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTCCTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGTCAAACTACTGCCTAAATCAGAATCCAGCAGGa                           <  5:189744/148‑1 (MQ=255)
                                                                                                                               ccGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTtata                    <  1:185315/149‑1 (MQ=255)
                                                                                                                               ccGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTtata                    <  1:15011/149‑1 (MQ=255)
                                                                                                                               ccGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTtat                     <  1:191067/148‑1 (MQ=255)
                                                                                                                                  tCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGCCCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGa                  >  5:262738/1‑148 (MQ=255)
                                                                                                                                  tCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGa                  >  3:153756/1‑148 (MQ=255)
                                                                                                                                     aaaTCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAac                                                      >  7:37101/1‑109 (MQ=255)
                                                                                                                                     aaaTCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAac                                                      <  8:37101/109‑1 (MQ=255)
                                                                                                                                             aaTCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTAttt      <  4:28745/149‑1 (MQ=255)
                                                                                                                                               tCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGCCCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTTcc    >  5:4647/1‑149 (MQ=255)
                                                                                                                                                cAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATATTATCTAtaaaat   <  7:323178/149‑6 (MQ=255)
                                                                                                                                                 aGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTTCCCa  <  3:155129/149‑1 (MQ=255)
                                                                                                                                                  gACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGc                                                                       >  8:265860/1‑79 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTCTTCAAACAATTCACGCAGCAAGGTAATAATTTGCGATACGGTATTTCTTATCTGTAATAAAAATTTCACCCGCAGACTTCTGTTATTCAAATTTCTGGTGTTACGCATGGTTTGATTGAAAATACCGTCGAAATCCTGAATCAGACGGGAGATATGGCCTACTTCGGCAATACCACTAAAATAATGTCGACCTTTATGGTTAAACCACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTTCCCA  >  NC_000913/1566274‑1566567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: