| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,004,644 | G→A | 6.9% | G258D (GGC→GAC) | fliD → | flagellar filament capping protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,004,644 | 0 | G | A | 6.9% | 178.0 / 2.7 | 58 | G258D (GGC→GAC) | fliD | flagellar filament capping protein |
| Reads supporting (aligned to +/- strand): ref base G (31/23); new base A (2/2); total (33/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.98e-01 | |||||||||||
GCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTT > NC_000913/2004496‑2004790 | gCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACCGCAGCAACACCATCCGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACggg > 1:258570/1‑149 (MQ=255) tGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAAcc < 4:120838/149‑1 (MQ=255) agcaACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCCGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGc > 5:194903/1‑148 (MQ=255) gcaACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCt > 7:244143/1‑148 (MQ=255) aaCGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAAc > 1:141109/1‑149 (MQ=255) ggTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCAt < 6:177770/149‑1 (MQ=255) aaTGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAAc < 7:128112/149‑1 (MQ=255) gcAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGACAACCAGACGCTAACCATCACTCAGGac < 8:228134/127‑1 (MQ=255) gcAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGACAACCAGACGCTAACCATCACTCAGGac > 7:228134/1‑127 (MQ=255) cAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCCCCCTGAACCTGAACGATGTCACCCCGGGCCACCAGACGCTAACCATCACTCAGGACCCCTCCAAAGCGCAACCGGCGag > 4:149635/1‑148 (MQ=255) aGCGGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGAtt < 3:149635/149‑1 (MQ=255) cTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTa > 1:161638/1‑148 (MQ=255) aCAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTaaa < 2:161638/147‑1 (MQ=255) aCAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTaaa < 2:141109/147‑1 (MQ=255) aGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGATAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACt > 8:332901/1‑149 (MQ=255) gTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTACCCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTg > 3:125670/1‑149 (MQ=255) aacaacGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGt < 5:310633/149‑1 (MQ=255) aacaacGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGt > 4:311542/1‑149 (MQ=255) aacaacGTCGCAATCGATCAAAGCAGCAACAACATCAGCGACGCGCTGGAAAACATCAACCTGAACCTTAACTATGTCAACACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGt < 6:194903/149‑1 (MQ=255) caacGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTg > 4:46561/1‑148 (MQ=255) acGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAAt > 2:50752/1‑149 (MQ=255) acGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAAt < 8:244143/149‑1 (MQ=255) gTCGCCATCGAGAATATCAGCAACACCATCAGCTACGCGCTGGAAAAAATCACCCTGAACCTGAACTATGTCACCACGGGCAACCAGCCGCTAACCATCACACAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGc > 4:8874/1‑149 (MQ=255) tCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAA‑CAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCt < 8:213144/149‑1 (MQ=255) gagaACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTc > 6:63244/1‑149 (MQ=255) gaACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCg > 3:269830/1‑148 (MQ=255) gaACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGc < 5:63244/149‑1 (MQ=255) gcaACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGAt < 3:46561/149‑1 (MQ=255) aaCACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATAc > 6:220578/1‑149 (MQ=255) cacCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCt > 4:225154/1‑149 (MQ=255) cATCAGCGACGCGTTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTca < 3:322069/149‑1 (MQ=255) cATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCaa > 4:254458/1‑97 (MQ=255) cATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCaa < 3:254458/97‑1 (MQ=255) cATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCtt > 3:168661/1‑147 (MQ=255) tCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTcagc < 7:131050/149‑1 (MQ=255) cgcgCTGGAAAACATCACCCTGACCCTGAACGATGTCACCACGGGCACCCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGa > 8:274460/1‑149 (MQ=255) cgcTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGAcc < 2:258570/149‑1 (MQ=255) cACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACAccgccg > 8:55070/1‑149 (MQ=255) aCCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACAccgccg < 4:125670/148‑1 (MQ=255) aCCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACAccgccg > 7:201973/1‑148 (MQ=255) cctgaacctgaaCGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACAccgccg > 6:179196/1‑146 (MQ=255) cctgaacctgaaCGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACAccg < 6:253930/143‑1 (MQ=255) cctgaacctgaaCGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACAccg > 5:253930/1‑143 (MQ=255) cctgaacGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCgg < 7:332901/149‑1 (MQ=255) cctgaacGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCgg < 3:311542/149‑1 (MQ=255) ctgaacGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCgg > 8:282431/1‑148 (MQ=255) gaacGATGTCACCACGGGCAACCAGACGCTACCCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAg > 3:190915/1‑149 (MQ=255) gaacGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACCCCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAAGGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAAGACACCGCCGTAGATGCGGGAg > 4:283986/1‑149 (MQ=255) gaacGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAg > 1:28856/1‑149 (MQ=255) aacGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGc > 8:326547/1‑149 (MQ=255) gATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTca > 1:50675/1‑109 (MQ=255) gATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTca < 2:50675/109‑1 (MQ=255) tGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATa < 5:220578/149‑1 (MQ=255) caccacGGACAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGc > 2:131907/1‑148 (MQ=255) accacGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGc > 4:97859/1‑147 (MQ=255) ccacGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGc > 8:230477/1‑146 (MQ=255) cacGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCaga < 1:50752/149‑1 (MQ=255) cacGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCa > 6:130486/1‑147 (MQ=255) cacGGACAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCaga < 1:131907/149‑1 (MQ=255) gggCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGtt < 7:230477/149‑1 (MQ=255) | GCCAGTGCCAGCAGCAACGGTATGGAGGTCTCGGTTGCCGCCCAGAATGCGCAGCTGACAGTCAACAACGTCGCCATCGAGAACAGCAGCAACACCATCAGCGACGCGCTGGAAAACATCACCCTGAACCTGAACGATGTCACCACGGGCAACCAGACGCTAACCATCACTCAGGACACCTCCAAAGCGCAAACGGCGATTAAAGACTGGGTGAATGCCTACAACTCGCTAATAGATACCTTCAGCAGCCTGACCAAATACACCGCCGTAGATGCGGGAGCTGATAGCCAGAGTT > NC_000913/2004496‑2004790 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |