Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,030,891 | G→A | 6.8% | R10C (CGC→TGC) | vsr ← | DNA mismatch endonuclease of very short patch repair |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,030,891 | 0 | G | A | 6.8% | 186.9 / 2.6 | 61 | R10C (CGC→TGC) | vsr | DNA mismatch endonuclease of very short patch repair |
Reads supporting (aligned to +/- strand): ref base G (28/28); new base A (2/2); total (30/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01 |
TCATCAACGACAAAATCCGGACGTCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGA > NC_000913/2030752‑2031034 | tcatcaACGACAAAATCCGGACGTCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGGGTGGCCt > 7:138788/1‑148 (MQ=255) tcaACGACAAAATCCGGACGTCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATc < 8:266797/149‑1 (MQ=255) aCGACAAAATCCGGACGTCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGt > 3:32203/1‑148 (MQ=255) cAAAATCCGGACGTCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGACCTTATCGTGAACg > 2:71644/1‑149 (MQ=255) tCCGGACGTCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGGGTGGCCTTATCGTGAACGTCGGc < 8:138788/149‑1 (MQ=255) tCCGGACGTCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGc > 1:198452/1‑149 (MQ=255) tCCGGACGTCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCAAGTGGCCTTATCGTg < 4:156882/140‑1 (MQ=255) tCCGGACGTCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCAAGTGGCCTTATCGTg > 3:156882/1‑140 (MQ=255) tCCGGACGTCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCAAGCGGCCTTATCGTg < 6:54411/140‑1 (MQ=255) tCCGGACGTCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCAAGAGGCCTTATCGTg > 5:54411/1‑140 (MQ=255) ccGGACGTCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGc > 7:292795/1‑148 (MQ=255) cGTCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCAtgtt < 7:561/149‑1 (MQ=255) tCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCAtgttgt < 6:150066/149‑1 (MQ=255) gACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTg < 2:198452/149‑1 (MQ=255) gCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGa < 6:8276/148‑1 (MQ=255) gCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGAc < 8:38971/149‑1 (MQ=255) gCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGACCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGa < 1:71644/148‑1 (MQ=255) tGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACg > 1:230945/1‑149 (MQ=255) tGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACg > 5:211590/1‑149 (MQ=255) gcaCGCCAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTCCCAATCGCGCGCATATTTTTTCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCATGCTGCCGCCACGc > 8:157073/3‑149 (MQ=255) gAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCg > 6:151380/1‑110 (MQ=255) gAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCg < 5:151380/110‑1 (MQ=255) gAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCAtgttg > 4:271319/1‑122 (MQ=255) gAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCAtgttg < 3:271319/122‑1 (MQ=255) gAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGc < 7:157073/149‑1 (MQ=255) tGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGt < 1:139867/149‑1 (MQ=255) aGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGa > 6:47891/1‑149 (MQ=255) gCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGAtt > 3:281833/1‑149 (MQ=255) ccTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCGGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGAtttt < 5:47891/149‑1 (MQ=255) cTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGAttttt < 2:225488/149‑1 (MQ=255) tGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTg > 8:29559/1‑149 (MQ=255) cccGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTg < 2:230945/147‑1 (MQ=255) ccGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCt > 6:283792/1‑149 (MQ=255) gTTAACAGAGTGGCGAGGGGATTCTCTTTCGCCGTATCACGAGTGGCAATCGCGCGGATATTTTTGCTGCGAGTGGCCTTATCGTTAACGTCGGCCATGTTGTGCCTCTTGCTGa < 8:95185/115‑1 (MQ=255) gTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGa > 7:95185/1‑115 (MQ=255) aaCAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAg > 8:96772/1‑147 (MQ=255) aCAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGa > 1:11593/1‑111 (MQ=255) aCAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGa < 2:11593/111‑1 (MQ=255) aGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGtt > 4:62868/1‑149 (MQ=255) ggCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGtt > 3:166012/1‑139 (MQ=255) ggCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGtt < 4:166012/139‑1 (MQ=255) ggCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACcgc < 7:29559/149‑1 (MQ=255) cgcTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTc < 4:85337/129‑1 (MQ=255) cgcTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTc > 3:85337/1‑129 (MQ=255) cgcTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCgg < 6:211590/149‑1 (MQ=255) tctcTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGATTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCa > 7:30412/1‑147 (MQ=255) tATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCACCGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGAcc > 1:107897/1‑149 (MQ=255) cGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCt > 2:348234/1‑99 (MQ=255) cGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCt < 1:348234/99‑1 (MQ=255) aTCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCctc > 3:94088/1‑73 (MQ=255) aTCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCctc < 4:94088/73‑1 (MQ=255) aCGCGTGGCAATCGCGCGCATATTTTTGCTGCGCGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCTTCAAATACAGGCACGaccacc < 4:281833/149‑1 (MQ=255) aCGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGaccacc > 3:255226/1‑149 (MQ=255) gcgTGCCAATCGCGCGCATCCTCTTGCTCCCAGCGGCCTTATCCGGCACGTCGCCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGCATCCCTTTTGGCTCAAGCAGGTTTGCCAGCGCGTATAAGACCTCGCCGACCACCGa < 8:292795/149‑1 (MQ=255) ctgtcgCAACCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGGCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCTCGGAAAAGTACGGCACGACCACCGa < 2:60813/144‑1 (MQ=255) tGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGa > 1:60813/1‑146 (MQ=255) gCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTAc > 3:293101/1‑149 (MQ=255) gcgcgcATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACt > 6:234574/1‑149 (MQ=255) gcgcATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTgg < 4:32203/149‑1 (MQ=255) ctgcGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGa < 7:96772/148‑1 (MQ=255) cggcGAGTGGCCTTATCGTGCACCTCGGCCATGTGGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGGTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGa < 4:70178/146‑1 (MQ=255) | TCATCAACGACAAAATCCGGACGTCCGGGCAGACTGGCGTCCTGAACGCGAAATGCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGA > NC_000913/2030752‑2031034 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |