Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,221,431 T→C 5.4% T187A (ACC→GCC)  bglX ← beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,221,4310TC 5.4% 224.4 / 2.9 75T187A (ACC→GCC) bglXbeta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (34/36);  new base C (2/2);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

TAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAGGCCATCATCTGCCGCTTCATAAGCAGAGACACGTCCGACC  >  NC_000913/2221283‑2221579
                                                                                                                                                    |                                                                                                                                                    
taATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGt                                                                                                                                                      <  8:316183/149‑1 (MQ=255)
 aaTCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATCGtt                                                                                                                                                     >  7:263479/1‑149 (MQ=255)
     aTTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCTCATTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCCCCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCCCCATGGTTTTAc                                                                                                                                                 >  8:110064/1‑149 (MQ=255)
     aTTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGCTTTAc                                                                                                                                                 >  7:196187/1‑149 (MQ=255)
     aTTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGCTTTAc                                                                                                                                                 >  7:191446/1‑149 (MQ=255)
     aTTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACACTGCATCGCTTCCACCATGGTTTTAc                                                                                                                                                 >  5:273783/1‑149 (MQ=255)
         aaCAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCAt                                                                                                                                             >  6:147425/1‑149 (MQ=255)
         aaCAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCAt                                                                                                                                             >  6:30585/1‑149 (MQ=255)
           cAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCAttg                                                                                                                                           <  4:324439/149‑1 (MQ=255)
                gcTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAg                                                                                                                                      <  1:160269/149‑1 (MQ=255)
                      gACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGt                                                                                                                                    >  4:51475/1‑145 (MQ=255)
                         tCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATAc                                                                                                                             >  6:294243/1‑149 (MQ=255)
                           atatCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACgg                                                                                                                           >  8:63299/1‑148 (MQ=255)
                           atatCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACg                                                                                                                            >  1:67587/1‑148 (MQ=255)
                            tatCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTa                                                                                                                          >  3:295518/1‑149 (MQ=255)
                                   ggTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGc                                                                                                                   <  8:344946/149‑1 (MQ=255)
                                      gttgtACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCa                                                                                                                 >  1:252984/1‑148 (MQ=255)
                                         gtACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAAc                                                                                                             >  5:226077/1‑149 (MQ=255)
                                          tACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAAcc                                                                                                            >  6:154942/1‑149 (MQ=255)
                                           aCTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCt                                                                                                           >  2:327458/1‑149 (MQ=255)
                                            ctctTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCtt                                                                                                          <  2:67587/149‑1 (MQ=255)
                                            ctctTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGGATCTTCGCCAAAACCtt                                                                                                          >  5:101592/1‑149 (MQ=255)
                                             tctTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCAtt                                                                                                                                            >  7:253627/1‑114 (MQ=255)
                                             tctTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCAtt                                                                                                                                            <  8:253627/114‑1 (MQ=255)
                                             tctTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTc                                                                                                         >  7:270773/1‑149 (MQ=255)
                                               tttACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTc                                                                                                         <  5:147425/147‑1 (MQ=255)
                                               tttACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTc                                                                                                         <  8:209679/147‑1 (MQ=255)
                                                  aCCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCTTTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGaa                                                                                                     <  3:51475/148‑1 (MQ=255)
                                                  aCCGCCTTCTACCGCGCCGAATGCGGCAAAGTGTTTGCCGCTGCTCATCACCGAGTAGCGATATGCCGTGCTGTTACCCTGCATCGCTTCCACCATGGTTTTACCCAGTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAg                                                                                                    >  2:223004/1‑149 (MQ=255)
                                                   ccGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCAACATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAg                                                                                                    <  6:273783/148‑1 (MQ=255)
                                                      ccTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACg                                                                                                >  2:82229/1‑149 (MQ=255)
                                                        ttCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTc                                                                                              >  5:42091/1‑149 (MQ=255)
                                                         tCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTcc                                                                                             >  5:316559/1‑149 (MQ=255)
                                                              cgcgCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAgc                                                                                        >  8:335910/1‑149 (MQ=255)
                                                                  ccGTATGCGGCAAAGTGTTTGACGCTGGTCTTCACCTAGTAGCGATCTTCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATGCTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGcgcg                                                                                     >  1:143801/1‑148 (MQ=255)
                                                                     tATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATc                                                                                 >  6:291387/1‑149 (MQ=255)
                                                                       tGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCCTTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATcg                                                                                <  5:334718/148‑1 (MQ=255)
                                                                         cGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATcgcgc                                                                             <  1:327458/149‑1 (MQ=255)
                                                                           gCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCga                                                                           <  6:42091/149‑1 (MQ=255)
                                                                           gCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCga                                                                           <  6:104835/149‑1 (MQ=255)
                                                                           gCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCga                                                                           <  2:252984/149‑1 (MQ=255)
                                                                            cAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATcgcgc                                                                             >  6:300801/1‑146 (MQ=255)
                                                                             aaaGTCTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTTCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTAGCCAATACCTTCGGAAGCTCGTCCCCAGCGCGGATCGCTCgaga                                                                         <  4:302576/149‑1 (MQ=255)
                                                                               aGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTg                                                                                                                                   <  1:214514/89‑1 (MQ=255)
                                                                               aGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTg                                                                                                                                   >  2:214514/1‑89 (MQ=255)
                                                                                 tgtTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATc                                                                     <  4:295518/149‑1 (MQ=255)
                                                                                      gACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAg                                                                                                    >  3:206433/1‑113 (MQ=255)
                                                                                      gACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAg                                                                                                    <  4:206433/113‑1 (MQ=255)
                                                                                             gTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGc                                                         <  8:206059/149‑1 (MQ=255)
                                                                                                 tcaCCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGCTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAg                                                     <  8:196187/149‑1 (MQ=255)
                                                                                                     cGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGCTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAg                                                     <  8:191446/145‑1 (MQ=255)
                                                                                                      gAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCa                                                 >  8:42984/1‑148 (MQ=255)
                                                                                                       aGTAGCGCTCTGCCGGGGTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCata                                               <  5:268923/149‑1 (MQ=255)
                                                                                                       aGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCata                                               >  7:131386/1‑149 (MQ=255)
                                                                                                        gTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCg                                                             <  2:84423/134‑1 (MQ=255)
                                                                                                        gTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCg                                                             >  1:84423/1‑134 (MQ=255)
                                                                                                           gCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCa                                           <  5:291387/149‑1 (MQ=255)
                                                                                                            cGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAg                                          <  5:154942/149‑1 (MQ=255)
                                                                                                             gATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGGCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAgg                                         <  7:110064/149‑1 (MQ=255)
                                                                                                                 tGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATAtt                                             >  2:35438/1‑141 (MQ=255)
                                                                                                                 tGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATAtt                                             <  1:35438/141‑1 (MQ=255)
                                                                                                                   ccGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAGGCcatca                                   <  8:131386/149‑1 (MQ=255)
                                                                                                                   ccGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAGGCca                                      <  5:30585/146‑1 (MQ=255)
                                                                                                                   ccGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGGGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAGGCcatca                                   <  5:294243/149‑1 (MQ=255)
                                                                                                                   ccGGGCTTTTACCCTGCATCGCTTCCACCATGGGTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAgg                                         <  2:142180/143‑1 (MQ=255)
                                                                                                                              cccTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAGGCCATCATCTGCCGCTTc                        <  6:316559/149‑1 (MQ=255)
                                                                                                                              cccTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAGGCCATCATCTGCCGCTTc                        <  6:281800/149‑1 (MQ=255)
                                                                                                                                     tCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAGGCCATCATCTGCCGCTTCATAAGCa                 <  7:169798/149‑1 (MQ=255)
                                                                                                                                       gCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAGGCCATCATCTGCCGCTTCATAAGCaga               <  8:270773/149‑1 (MQ=255)
                                                                                                                                              ccaTGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACGTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATGGGTGCCCAGGTCATATTCAGGCCATCATCTGCCGCTTCATAAGCAGAGACACGt        <  8:263479/149‑1 (MQ=255)
                                                                                                                                              ccaTGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAGGCCATCATCTGCCGCTTCATAAGCAGAGACACGt        >  5:138639/1‑149 (MQ=255)
                                                                                                                                              ccaTGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAGGCCATCATCTGCCGCTTCATAAGCAGAGACACGt        >  8:89050/1‑149 (MQ=255)
                                                                                                                                              ccaTGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGAGCGCGCGAGACATCGACCATCGGTGCCCAGGTCat                                                <  2:79498/109‑1 (MQ=255)
                                                                                                                                               caTGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAgg                                                    <  2:197675/104‑1 (MQ=255)
                                                                                                                                               caTGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAgg                                                    >  1:197675/1‑104 (MQ=255)
                                                                                                                                                   gTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCTGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAGGCCATCATCTGCCGCTTAATAAGCAGAGACACGTCCTAc   >  3:27333/1‑149 (MQ=255)
                                                                                                                                                   gTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAGGCCATCATCTGCCGCTTCATAAGCAGAGACACGTCCGa    <  6:226077/148‑1 (MQ=255)
                                                                                                                                                    ttttACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAGGCCATCATCTGCCGCTTCATAAGCAGAGACACGTCCGAcc  >  5:179461/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TAATCATTAAACAGGCGCTGCGGACTCATATCGACGGTGTTGTACTCTTTACCGCCTTCTACCGCGCCGTATGCGGCAAAGTGTTTGACGCTGGTCATCACCGAGTAGCGATCTGCCGGGCTTTTACCCTGCATCGCTTCCACCATGGTTTTACCCATTGTTGAGGTGAGATACGTATCTTCGCCAAAACCTTCGGAAGCACGTCCCCAGCGCGGATCGCGCGAGACATCGACCATCGGTGCCCAGGTCATATTCAGGCCATCATCTGCCGCTTCATAAGCAGAGACACGTCCGACC  >  NC_000913/2221283‑2221579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: