Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,550,574 C→G 5.0% intergenic (‑29/+67) yfeX ← / ← yfeY porphyrinogen oxidase, cytoplasmic/RpoE‑regulated lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,550,5740CG 5.0% 244.2 / 2.9 73intergenic (‑29/+67)yfeX/yfeYporphyrinogen oxidase, cytoplasmic/RpoE‑regulated lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (35/33);  new base G (2/2);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GCAAATGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCACG  >  NC_000913/2550433‑2550718
                                                                                                                                             |                                                                                                                                                
gCAAATGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGt                                                                                                                                           >  7:26772/1‑149 (MQ=255)
  aaaTGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCCATCCGTgg                                                                                                                                         >  3:70422/1‑149 (MQ=255)
  aaaTGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTgg                                                                                                                                         >  4:164268/1‑149 (MQ=255)
     tGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGGATTGCGTGGCAt                                                                                                                                      <  4:201236/149‑1 (MQ=255)
       ttttACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCAttg                                                                                                                                    >  8:52161/1‑149 (MQ=255)
        tttACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGGATTGCGTGGCAtta                                                                                                                                    <  1:150153/148‑2 (MQ=255)
            cTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCATATCCCCGCCCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGATACATTGTTCCTCCTGAAAATAATAATTCCACGCAATCCGTGTCATTGTTGa                                                                                                                                <  6:25417/148‑1 (MQ=255)
                 cgcacgcaGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTa                                                                                                                          >  4:138124/1‑149 (MQ=255)
                 cgcacgcaGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTa                                                                                                                          <  3:118863/149‑1 (MQ=255)
                    acgcaGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTAttt                                                                                                                       >  4:195619/1‑149 (MQ=255)
                       caGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTAc                                                                                                                    <  3:53899/149‑1 (MQ=255)
                       caGGGCGTCAACTTCCCCTTTCACGTGGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGAAAAATGCCCCTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCCCGCAATCCGTGGCATTGTTGAGCGCTATTTTAc                                                                                                                    <  4:202255/149‑1 (MQ=255)
                            cGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGtt                                                                                                               <  3:262477/149‑1 (MQ=255)
                               cAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGttttt                                                                                                            >  8:336817/1‑149 (MQ=255)
                                 aCTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTc                                                                                                          <  1:297890/149‑1 (MQ=255)
                                   ttCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTc                                                                                                          >  3:118469/1‑147 (MQ=255)
                                   ttCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTc                                                                                                          <  4:118469/147‑1 (MQ=255)
                                        cTTTCACGTCGGCTTCGATCCAAATCGCCGCTCGGCAATGTTCTGGCAAACTGCCACTCTGAACCTGACACATTGATCCTCCTGAAAATATTAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATATTACGTGTTTTTTCgctgctg                                                                                                   <  2:77338/149‑3 (MQ=255)
                                           tCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGcggcggcgg                                                                                                >  1:225721/1‑149 (MQ=255)
                                             aCGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGt                                                                                               <  5:326009/148‑1 (MQ=255)
                                             aCGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGt                                                                                               <  3:138124/148‑1 (MQ=255)
                                               gTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTg                                                                                            >  2:230686/1‑149 (MQ=255)
                                               gTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTg                                                                                            >  4:148024/1‑149 (MQ=255)
                                                 tGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACGTTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGtt                                                                                          <  3:184109/149‑1 (MQ=255)
                                                   gCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGtt                                                                                          >  1:13887/1‑147 (MQ=255)
                                                   gCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTc                                                                                         <  2:178762/148‑1 (MQ=255)
                                                          tCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCa                                                                                  >  1:343456/1‑148 (MQ=255)
                                                             aaaTCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAAt                                                                               >  6:132638/1‑148 (MQ=255)
                                                               aTCGCCGCGCGCCAATGATCTGGCAAACTGCCACTCAGCACCTGCGACATTGTTCCTCCTGAAAATAATACTCCCCCCCAAGCCGGCGCTTTGTTGAGCGCTATGTACCCGGCCTTTCCGCCGCGGCGGTCGGTTCCCACGCCAATTaa                                                                            <  4:70384/149‑1 (MQ=255)
                                                                         ggCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAAcgccgc                                                                      >  6:335137/1‑145 (MQ=255)
                                                                         ggCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGa                                                                  <  3:164268/149‑1 (MQ=255)
                                                                            aaTGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAATTAATAATGCCACGAAAGCCGTGGCATTGTTGAGCGGTATTTTACGTGTTTTTTCCCGCCGCCGTTTTGTACAGAGGCAAATTACCGCCCCAAGaaaa                                                               >  4:135449/1‑149 (MQ=255)
                                                                            aaTGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATaa                                                               >  2:219204/1‑149 (MQ=255)
                                                                                   tGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCGCGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAAGTAACGCCGCCAGATAATTTTACt                                                        >  4:296642/1‑149 (MQ=255)
                                                                                     gCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGtt                                                                                          >  6:51879/1‑113 (MQ=255)
                                                                                     gCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGtt                                                                                          <  5:51879/113‑1 (MQ=255)
                                                                                     gCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACt                                                        <  8:26772/147‑1 (MQ=255)
                                                                                     gCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGa                                                      <  1:230686/149‑1 (MQ=255)
                                                                                             ccACTCTGCACCTGAGCCATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTATACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCa                                               <  4:207694/148‑1 (MQ=255)
                                                                                                 tctGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGtttt                                          >  8:146660/1‑149 (MQ=255)
                                                                                                   tGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGtttt                                          <  7:89084/147‑1 (MQ=255)
                                                                                                     gaCCTGAGTAATTGTTACTGCTGTAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCa                                       <  3:135449/147‑1 (MQ=255)
                                                                                                     aaCCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGttttt                                         <  7:146660/146‑1 (MQ=255)
                                                                                                     aaCCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCa                                       <  5:132638/148‑1 (MQ=255)
                                                                                                     aaCCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAg                                      >  8:96055/1‑149 (MQ=255)
                                                                                                     aaCCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAg                                      >  5:314783/1‑149 (MQ=255)
                                                                                                      aCCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAgg                                     <  5:335137/149‑1 (MQ=255)
                                                                                                      aCCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAgg                                     <  3:195619/149‑1 (MQ=255)
                                                                                                         tGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGtt                                            >  4:153853/1‑139 (MQ=255)
                                                                                                         tGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGtt                                            <  3:153853/139‑1 (MQ=255)
                                                                                                         tGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTa                                  >  6:105563/1‑149 (MQ=255)
                                                                                                          gagaCATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTAt                                 >  4:222653/1‑149 (MQ=255)
                                                                                                           agaCATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATc                                <  5:105563/149‑1 (MQ=255)
                                                                                                               aTTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTTACGCCGCCAGATAATTTTACTGACTATCCAGTTTTTCAGGTTATCGTcc                            >  6:304685/1‑149 (MQ=255)
                                                                                                                      ctcctGAAAATAATAATGCCACGCAATCCTTTGCATTTTTTATCGCTATTTTACGTGTTTTTTCGCGGCGTCGTTTTGTTCATACGCAAATTAACGCCTCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGc                      >  2:223869/1‑148 (MQ=255)
                                                                                                                      ctcctGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGc                      <  7:96055/148‑1 (MQ=255)
                                                                                                                       tcctGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCAt                    >  8:230535/1‑149 (MQ=255)
                                                                                                                         ctGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGTGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAgg                                     <  6:179349/130‑1 (MQ=255)
                                                                                                                         ctGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGTGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAgg                                     >  5:179349/1‑130 (MQ=255)
                                                                                                                           gAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTagc                >  3:38094/1‑147 (MQ=255)
                                                                                                                            aaaaTAATAATGCCACGCAATCCGTGTCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAAc                <  2:22410/148‑1 (MQ=255)
                                                                                                                            aaaaTAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAAc                <  3:222653/148‑1 (MQ=255)
                                                                                                                            aaaaTAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAAc                <  4:233594/148‑1 (MQ=255)
                                                                                                                             aaaTAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAAccc              >  4:133202/1‑149 (MQ=255)
                                                                                                                               tcaacaatGCCACGGATTGCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCtt            >  7:296612/6‑149 (MQ=255)
                                                                                                                               tcaacaatGCCACGGATTGCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCtt            >  3:190573/6‑149 (MQ=255)
                                                                                                                                 aataatGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCAtt            >  2:292893/1‑147 (MQ=255)
                                                                                                                                  ataatGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAg         >  7:328698/1‑149 (MQ=255)
                                                                                                                                  ataatGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAg         <  1:219204/149‑1 (MQ=255)
                                                                                                                                      tGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTa                                                          >  1:194540/1‑96 (MQ=255)
                                                                                                                                      tGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTa                                                          <  2:194540/96‑1 (MQ=255)
                                                                                                                                        ccACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCAc   >  6:293414/1‑149 (MQ=255)
                                                                                                                                         cACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCACg  >  7:170599/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                
GCAAATGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCACG  >  NC_000913/2550433‑2550718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: