Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,551,532	C→T	6.0%	L65L (CTG→CTA)	yfeZ ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,551,532	0	C	T	6.0%	213.6 / 2.7	67	L65L (CTG→CTA)	yfeZ	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (31/32);  new base T (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

TTCACCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGG  >  NC_000913/2551385‑2551679                                                                                                                                                    |                                                                                                                                                    ttCACCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCa                                                                                                                                                    <  3:183986/149‑1 (MQ=255)    aCCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCCCCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGcc                                                                                                                                                 >  4:91142/1‑149 (MQ=255)    aCCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGTAAACGTAGCCCGCCGACCGCTCAAACACAACGCCAGCACCGCCGGAATGCCAGGAATCAGcc                                                                                                                                                 >  8:267384/1‑149 (MQ=255)    aCCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCCGCACCGCCGGAATGCCAGGAATCAGcc                                                                                                                                                 >  3:27024/1‑149 (MQ=255)    aCCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGcc                                                                                                                                                 >  7:25799/1‑149 (MQ=255)    aCCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCGCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGc                                                                                                                                                  <  5:211911/148‑1 (MQ=255)                    cacGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATc                                                                                                                                 <  4:321562/149‑1 (MQ=255)                     acGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCg                                                                                                                                >  7:222298/1‑149 (MQ=255)                       ggTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAACCGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTTTCGTg                                                                                                                              >  5:118772/1‑149 (MQ=255)                            ccAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAAGCAGCCCCAGCCAGAAATTATCGTGATCgg                                                                                                                         <  3:105199/149‑1 (MQ=255)                             cAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCgg                                                                                                                         >  7:210158/1‑148 (MQ=255)                              aGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGa                                                                                                                       <  8:318406/149‑1 (MQ=255)                               gCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGCCCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATTAGCCCCAGCCAGaa                                                                                                                                       >  3:314/1‑132 (MQ=255)                               gCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGTAATTAGCCCCAGCCAGaa                                                                                                                                       <  4:314/132‑1 (MQ=255)                               gCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATTAGCCCCAGCCAGaa                                                                                                                                       <  8:184691/132‑1 (MQ=255)                               gCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATTAGCCCCAGCCAGaa                                                                                                                                       >  7:184691/1‑132 (MQ=255)                                cAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGaa                                                                                                                                       <  7:229073/131‑1 (MQ=255)                                cAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGaa                                                                                                                                       >  8:229073/1‑131 (MQ=255)                                  aaGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATaa                                                                                                                    >  2:296087/1‑148 (MQ=255)                                     tAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATaaac                                                                                                                  >  5:86884/1‑146 (MQ=255)                                            accTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTag                                                                                                         <  6:192350/149‑1 (MQ=255)                                              cttcgcCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCAGCCGACCGCTCAACAAAAACGCCAGAACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTagca                                                                                                       <  2:77687/146‑1 (MQ=255)                                               tGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTagcag                                                                                                      >  7:71459/1‑149 (MQ=255)                                                   ccAACAGCATCAGAAACAAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCGGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCa                                                                                                  <  6:135163/149‑1 (MQ=255)                                                   ccAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGCGATAAACCGGATTTAGCAgcg                                                                                                    >  7:333001/1‑147 (MQ=255)                                                    caacagcaacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCCGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAg                                                                                                 >  3:314388/1‑149 (MQ=255)                                                    caacagcaacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAg                                                                                                 >  2:335894/1‑149 (MQ=255)                                                        agcaacagAAAATAGAGTACACGCCAGGTGCGAGTAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTAcc                                                                                             <  4:27024/149‑1 (MQ=255)                                                          caacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTagc                                                                                                        <  7:129690/136‑1 (MQ=255)                                                          caacagAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTagc                                                                                                        >  8:129690/1‑136 (MQ=255)                                                           aacagAAAATAGAGTACCCGCCATGTGCGAGGACACGTAGACCGCCGACCGCTCAACAAACACTCCAGCACCGCAGGAATGCCAGGAATCAGCCCCAGCCAGAAATAATCTTGCTCTGGAGAAAACAGATTTAGCAGAGCAGTatacag                                                                                          >  5:131434/1‑144 (MQ=255)                                                              agAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCCCAGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTc                                                                                       >  5:161749/1‑149 (MQ=255)                                                                 aaaTAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTcgcg                                                                                    >  8:147344/1‑149 (MQ=255)                                                                  aaTAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGTAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTcgcgc                                                                                   >  6:242835/1‑149 (MQ=255)                                                                   aTAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTcgcgc                                                                                   >  2:263244/1‑148 (MQ=255)                                                                    tAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGa                                                                                 <  5:242835/149‑1 (MQ=255)                                                                    tAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGa                                                                                 <  8:25799/149‑1 (MQ=255)                                                                      gagTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGAc                                                                                <  3:91142/148‑1 (MQ=255)                                                                            acGCCAGGTTCGAGGAAACGTAGCCAGCAGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCAAAGCCAGAAATTATCGTGATATGGAAAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCgg                                                                         >  4:37013/1‑149 (MQ=255)                                                                             cGCCAGGGGCGAGGAAACGTAGCCCGCCGACCGCGCAACAAACACCCCCGCACCGCCGTAATGCCAGGAAGCAGCCCCAGCCAGAAATTATCGTGCTCGGGATAAAACAGCTTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGc                                                                        <  6:118772/149‑1 (MQ=255)                                                                                cAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAAt                                                                     <  8:210158/149‑1 (MQ=255)                                                                                  ggTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGa                                                                   >  7:183138/1‑149 (MQ=255)                                                                                   gTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACACTGCTCGCGCGACGCACCGGCAATGa                                                                   >  3:41435/1‑148 (MQ=255)                                                                                     gCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCGATGACaa                                                                <  6:86884/149‑1 (MQ=255)                                                                                       gAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAAc                                                              <  1:12311/149‑1 (MQ=255)                                                                                        aGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAAc                                                              <  3:154439/148‑1 (MQ=255)                                                                                        aGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAAc                                                              >  4:154439/1‑148 (MQ=255)                                                                                        aGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACa                                                             <  1:158944/149‑1 (MQ=255)                                                                                         ggAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAg                                                            >  3:61233/1‑149 (MQ=255)                                                                                                  gCCCGCCGCCCGCGCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCCGCCAGAAATAATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGt                                                   >  5:336804/1‑149 (MQ=255)                                                                                                       ccgACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCCGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCAGCTCGCGCGACGCACGGGCAATGACAAACATCACCCAGGATcgcg                                              >  4:214111/1‑149 (MQ=255)                                                                                                        cgACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCgcgc                                             <  1:263244/149‑1 (MQ=255)                                                                                                              cTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAg                                       <  7:147344/149‑1 (MQ=255)                                                                                                              cTCAACAAAAACGACAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAg                                       <  7:267384/149‑1 (MQ=255)                                                                                                                 aacaaAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCaa                                    <  1:296087/149‑1 (MQ=255)                                                                                                                   caaAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGaa                                        >  6:54453/1‑143 (MQ=255)                                                                                                                   caaAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGaa                                        <  5:54453/143‑1 (MQ=255)                                                                                                                    aaaaaCGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAg                                 >  5:50247/1‑149 (MQ=255)                                                                                                                     aaaaCGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGaa                                        <  5:324393/141‑1 (MQ=255)                                                                                                                     aaaaCGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGaa                                        >  6:324393/1‑141 (MQ=255)                                                                                                                          gCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAg                           >  2:164196/1‑149 (MQ=255)                                                                                                                                 ccgccgGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCagaag                    <  4:61233/149‑1 (MQ=255)                                                                                                                                        aaTGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCTCCTGAAGCAATAGCACAAGCCAGAAGCGCAAgg             <  6:161749/149‑1 (MQ=255)                                                                                                                                        aaTGCCAGGAATCAGCCCCAGACAGATATTATCGTGATCGGGATATATCAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAACCAGGACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCaa               <  3:37013/147‑1 (MQ=255)                                                                                                                                          tGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGt           <  1:335894/149‑1 (MQ=255)                                                                                                                                                   tCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAgg  <  6:336804/149‑1 (MQ=255)                                                                                                                                                   tCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTGCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAgg  <  8:183138/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TTCACCGTTCAGCCACAGCCACGGTTGCCAGCAAAGTAAAACCACCTGCGCCAACAGCAACAGAAAATAGAGTACACGCCAGGTGCGAGGAAACGTAGCCCGCCGACCGCTCAACAAAAACGCCAGCACCGCCGGAATGCCAGGAATCAGCCCCAGCCAGAAATTATCGTGATCGGGATAAAACAGATTTAGCAGCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGG  >  NC_000913/2551385‑2551679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑