Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,890,619 T→A 8.1% D427V (GAC→GTC)  cysJ ← sulfite reductase, alpha subunit, flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,890,6190TA 8.1% 193.3 / 2.9 74D427V (GAC→GTC) cysJsulfite reductase, alpha subunit, flavoprotein
Reads supporting (aligned to +/- strand):  ref base T (33/32);  new base A (3/4);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGCGCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGAT  >  NC_000913/2890472‑2890766
                                                                                                                                                   |                                                                                                                                                   
gcggcgCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTc                                                                                                                                                    <  2:69951/149‑1 (MQ=255)
   gcgcGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGc                                                                                                                                                 <  3:171403/149‑1 (MQ=255)
     gcgTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGa                                                                                                                                               <  4:306120/149‑1 (MQ=255)
     gcgTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGa                                                                                                                                               <  3:131131/149‑1 (MQ=255)
       gTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAgg                                                                                                                                             <  4:296902/149‑1 (MQ=255)
       gTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAg                                                                                                                                              >  5:191015/1‑148 (MQ=255)
         tgctgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGaa                                                                                                                                           <  7:155149/149‑1 (MQ=255)
          gctgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGaa                                                                                                                                           <  8:192905/148‑1 (MQ=255)
          gctgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGaa                                                                                                                                           <  2:168884/148‑1 (MQ=255)
           ctgcATATAGGCGCGGAACGGAGCAATACCGGTGCCTGGGGCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCTCCCTCTTCTTCCTCGCTGACCGCGTGGAAg                                                                                                                                          <  6:103252/148‑1 (MQ=255)
              cATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTg                                                                                                                                       <  6:197247/148‑1 (MQ=255)
              cATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTAGCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTg                                                                                                                                       <  8:307778/148‑1 (MQ=255)
               aTAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGaa                                                                                                                                           <  2:73414/143‑1 (MQ=255)
               aTAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGaa                                                                                                                                           >  1:73414/1‑143 (MQ=255)
                tAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAg                                                                                                                                    <  7:253547/149‑1 (MQ=255)
                  aaGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAgg                                                                                                                                   <  4:128428/148‑1 (MQ=255)
                  aaGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGGTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAgg                                                                                                                                   <  7:210048/148‑1 (MQ=255)
                    ggCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGcac                                                                                                                                <  8:10547/149‑1 (MQ=255)
                       gcgGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTACACGCGGTCAGCGAGGAAGCTGGAGGcaccac                                                                                                                             >  5:276881/1‑149 (MQ=255)
                            aCGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCGTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACGACCGGCa                                                                                                                        <  8:86169/149‑1 (MQ=255)
                            aCGGCGCAATACAGGTTCCTGTGCAAATCATAATCACCGGGGTTTCTGGATTGGCTTGCAGGCGGAAGTTATCGTTAAGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCa                                                                                                                        <  8:81318/149‑1 (MQ=255)
                                          gTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTc                                                                                                          >  3:107889/1‑149 (MQ=255)
                                                       tCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACg                                                                                              >  3:202965/1‑148 (MQ=255)
                                                        cATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGca                                                                                            >  4:239534/1‑149 (MQ=255)
                                                         aTAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGca                                                                                            <  1:75538/148‑1 (MQ=255)
                                                          tAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcac                                                                                           >  6:179730/1‑148 (MQ=255)
                                                                     gTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGt                                                                               <  6:191015/149‑1 (MQ=255)
                                                                         cTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGAc                                                                            <  1:75961/148‑1 (MQ=255)
                                                                          tGGATTGGCTGGCAGGCGGAAGTTAGCGGTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGATGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAAGGCACCCCCCCAACGGTGACgt                                                                          >  6:265000/1‑149 (MQ=255)
                                                                            gATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACgtg                                                                         >  5:338183/1‑148 (MQ=255)
                                                                            gATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTACTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACgtg                                                                         >  8:218831/1‑148 (MQ=255)
                                                                                 gctggcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTc                                                                   >  2:245876/1‑149 (MQ=255)
                                                                                 gctggcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTc                                                                   >  3:232889/1‑149 (MQ=255)
                                                                                 gctggcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGGCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACCCCAACGGGGACGTGTACTTc                                                                   <  7:243299/149‑1 (MQ=255)
                                                                                   tggcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGt                                                                 >  3:327164/1‑149 (MQ=255)
                                                                                     gcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTtc                                                               >  8:248652/1‑149 (MQ=255)
                                                                                       aGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACATCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGTCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTtctc                                                             <  4:4859/149‑1 (MQ=255)
                                                                                        ggCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCg                                                            <  8:132009/149‑1 (MQ=255)
                                                                                                   tCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcaccacac                                                                                      >  1:344916/1‑112 (MQ=255)
                                                                                                   tCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcaccacac                                                                                      <  2:344916/112‑1 (MQ=255)
                                                                                                      ttATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGACACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGc                                                >  4:290801/1‑147 (MQ=255)
                                                                                                        aTGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACAACCGGCACGGGCGCGGCCTTACACGTCGTAACGCACCACACAAACGTTGACGTGTAATTCGTTGTCGACTTCCGCCTGCgag                                             >  6:237636/1‑148 (MQ=255)
                                                                                                          gTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGGCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCgagga                                           <  6:213270/148‑1 (MQ=255)
                                                                                                           ttCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACTGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGaggagg                                         <  2:132189/149‑1 (MQ=255)
                                                                                                             cGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACCCCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGc                                        >  5:222300/1‑148 (MQ=255)
                                                                                                              gATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGa                                      >  1:202599/1‑149 (MQ=255)
                                                                                                              gATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGAGAGGAAGCTGGATGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAAAGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGa                                      >  1:207654/1‑149 (MQ=255)
                                                                                                               aTAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGAt                                     <  2:200273/149‑1 (MQ=255)
                                                                                                                tAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATg                                    <  4:202965/149‑1 (MQ=255)
                                                                                                                  aaTACGCGGACTTCGCCCTCTTCTTCCACGAGGACCGCGCGCAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCAACGTCGTAACGCACCACACCAACGGTGACGTGTACTGCGTTCTCGACTTCCGCCTGCGAGGAGGCGATgg                                   >  8:112115/1‑148 (MQ=255)
                                                                                                                     aCGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATa                               >  7:286832/1‑149 (MQ=255)
                                                                                                                       gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCCCGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATa                               >  3:206333/1‑147 (MQ=255)
                                                                                                                       gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGACACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACa                             <  3:290801/149‑1 (MQ=255)
                                                                                                                        cgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAg                            <  7:331323/149‑1 (MQ=255)
                                                                                                                          gACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAc                          >  7:275145/1‑149 (MQ=255)
                                                                                                                          gACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAc                          <  4:232889/149‑1 (MQ=255)
                                                                                                                             ttCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcgcg                       >  6:113841/1‑149 (MQ=255)
                                                                                                                             ttCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcgcg                       >  5:75154/1‑149 (MQ=255)
                                                                                                                               cGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcgcg                       <  8:275145/147‑1 (MQ=255)
                                                                                                                                gCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACgcggc                     <  5:179730/148‑1 (MQ=255)
                                                                                                                                 cccTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGt                   <  6:222300/149‑1 (MQ=255)
                                                                                                                                   ctctTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGt                   >  7:70471/1‑147 (MQ=255)
                                                                                                                                    tcttctTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAAGAGGCGATGGAATCAAGACGCGGCGTCAg                >  8:238764/1‑149 (MQ=255)
                                                                                                                                     cttcttCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGCCGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGc               >  1:301897/1‑149 (MQ=255)
                                                                                                                                      ttcttcCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAg                <  6:338183/147‑1 (MQ=255)
                                                                                                                                         ttcCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCAACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCAGCCTGCGAGGAGGCGATGGAATACAGACGCGTCGTCAGCGGgc           >  5:107300/1‑149 (MQ=255)
                                                                                                                                         ttcCACGCGGACAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTACTCGACTTACGCCAGCGAGCAGGCGATGGAAGACAGACGCGGCGTCAGCGGgc           >  4:214543/1‑149 (MQ=255)
                                                                                                                                          tcCCCGCGGTCAGCGCGGAAGCTGGAGGCCCGCCCGGCGCGGGCGCGGCCTGCCCCGTCGTACCGGGCCACCCCAACGGTGACGTGTACTTCGGGCGCGCCTTCCGCCTGCGAGGCGGCGAGGGAAGACAGACGCGGCGTCAGCGGccg          >  5:79521/1‑149 (MQ=255)
                                                                                                                                          tcCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGCCGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGgcg          >  5:79519/1‑149 (MQ=255)
                                                                                                                                          tcCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGgcg          >  4:176406/1‑149 (MQ=255)
                                                                                                                                          tcCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGACTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGgcg          >  5:132782/1‑149 (MQ=255)
                                                                                                                                            cACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCgca        <  7:248652/149‑1 (MQ=255)
                                                                                                                                            cACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGCTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGgcg          >  7:276407/1‑147 (MQ=255)
                                                                                                                                                  gTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGAACAACACCAACGGTGACGTTTACTGCGTTCTCGACTTCCGCCTGCTCAGATGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGat  <  7:238764/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCGGCGCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGAT  >  NC_000913/2890472‑2890766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: