Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,305,974 C→T 5.1% A629A (GCG→GCA deaD ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,305,9740CT 5.1% 251.3 / 2.1 78A629A (GCG→GCAdeaDATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (36/37);  new base T (2/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01

TCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGAC  >  NC_000913/3305829‑3306116
                                                                                                                                                 |                                                                                                                                              
tcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGATAAAAAGCCCCGATGGTAAAAATGGGGGCTGGATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCa                                                                                                                                             >  8:257853/1‑149 (MQ=255)
tcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCa                                                                                                                                             >  7:346479/1‑149 (MQ=255)
tcCAATGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCa                                                                                                                                             >  1:9307/1‑149 (MQ=255)
  cAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGc                                                                                                                                              <  8:336232/146‑1 (MQ=255)
  cAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCAt                                                                                                                                            <  7:31360/148‑1 (MQ=255)
  cAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATc                                                                                                                                           <  5:107967/149‑1 (MQ=255)
    gTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcac                                                                                                                                         >  5:47796/1‑149 (MQ=255)
    gTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcac                                                                                                                                         >  7:182009/1‑149 (MQ=255)
     tGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcac                                                                                                                                         >  8:247703/1‑148 (MQ=255)
       aaacaTAGTGCGACTTCGTTGTTATGCATTGCACTGTACCAGTACACGAGTAAAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcacca                                                                                                                                       <  2:308541/148‑1 (MQ=255)
        aaaaTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcaccac                                                                                                                                      <  4:291500/148‑1 (MQ=255)
        aaaaTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCaccacc                                                                                                                                     <  8:236111/149‑1 (MQ=255)
                  gACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacga                                                                                                                           <  7:323297/149‑1 (MQ=255)
                     tGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAAcgacgacga                                                                                                                        >  4:301255/1‑149 (MQ=255)
                       cGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACgacgacgac                                                                                                                       <  6:47796/148‑1 (MQ=255)
                         ttgttATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCgg                                                                                                                    <  8:182009/149‑1 (MQ=255)
                                  aTTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTATGCATCACCACCGAAACGACGACGACCGGTAGAatcat                                                                                                           >  1:276211/1‑149 (MQ=255)
                                  aTTGCACTGTACCAGTACACGAGAACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcat                                                                                                           <  6:288940/149‑1 (MQ=255)
                                               aGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAgc                                                                                              <  4:273008/149‑1 (MQ=255)
                                                gTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGAAGAATCATCACGACGCGGAgcg                                                                                             >  5:264601/1‑149 (MQ=255)
                                                   cacGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGcgcg                                                                                           <  3:301255/148‑1 (MQ=255)
                                                   cacGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGcgcg                                                                                           <  8:282489/148‑1 (MQ=255)
                                                    acGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAac                                                                                                                                >  6:98343/1‑110 (MQ=255)
                                                    acGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAac                                                                                                                                <  5:98343/110‑1 (MQ=255)
                                                        gTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGACtt                                                                                      >  1:92935/1‑148 (MQ=255)
                                                                gACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGtt                                                                             >  6:274307/1‑149 (MQ=255)
                                                                 aCAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTc                                                                            >  3:212130/1‑149 (MQ=255)
                                                                   aGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTTTATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGCCCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgc                                                                          >  5:253464/1‑149 (MQ=255)
                                                                   aGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTCACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgc                                                                          >  3:265674/1‑149 (MQ=255)
                                                                    gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACAACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGAGCGGCCTTCACGACGTTcgca                                                                         >  1:30670/1‑148 (MQ=255)
                                                                     aaaaaaaGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgccg                                                                        >  2:194441/1‑149 (MQ=255)
                                                                       aaaaaGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCt                                                                      <  5:144983/149‑1 (MQ=255)
                                                                         aaaGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCac                                                                                   >  2:62203/1‑134 (MQ=255)
                                                                         aaaGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCac                                                                                   <  1:62203/134‑1 (MQ=255)
                                                                           aGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTaaaa                                                                  >  5:129108/1‑149 (MQ=255)
                                                                           aGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTaaaa                                                                  >  3:155372/1‑149 (MQ=255)
                                                                           aGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGCAGCGCGGCCTTCACGACGTTCGCCGCTaaaa                                                                  <  5:254586/149‑1 (MQ=255)
                                                                             ccccGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAacg                                                                <  7:257853/149‑1 (MQ=255)
                                                                               ccGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTa                                                                                                                  <  3:299652/97‑1 (MQ=255)
                                                                               ccGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAacgac                                                              <  7:287732/149‑1 (MQ=255)
                                                                               ccGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAacgac                                                              >  7:207207/1‑149 (MQ=255)
                                                                               ccGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAacgac                                                              <  2:9307/149‑1 (MQ=255)
                                                                                     gTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTATGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCAt                                                        <  2:276211/149‑1 (MQ=255)
                                                                                     gTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAAAGACTACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCAt                                                        >  1:351692/1‑149 (MQ=255)
                                                                                      taattaTCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCTGAGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGTCGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATc                                                       <  6:264601/144‑1 (MQ=255)
                                                                                      tAAAAATCGGGGGTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGAAGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATc                                                       <  1:176744/149‑1 (MQ=255)
                                                                                      tAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATc                                                       <  1:194441/149‑1 (MQ=255)
                                                                                           aTCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATcaccac                                                  <  6:99254/149‑1 (MQ=255)
                                                                                              ggggCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACgg                                                <  1:322243/148‑1 (MQ=255)
                                                                                                ggCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCa                                             >  1:315205/1‑149 (MQ=255)
                                                                                                      atatatTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCa                                       >  6:195127/1‑149 (MQ=255)
                                                                                                       tatatTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTATGCATCACCACCGAAACGACGACGACCGGTAGaa                                                                                                               >  6:128244/1‑76 (MQ=255)
                                                                                                       tatatTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTATGCATCACCACCGAAACGACGACGACCGGTAGaa                                                                                                               <  5:128244/76‑1 (MQ=255)
                                                                                                       tatatTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCa                                       <  2:315205/148‑1 (MQ=255)
                                                                                                       tatatTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCAc                                      <  8:346479/149‑1 (MQ=255)
                                                                                                          attattTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGCATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGACGCTAAAACGACGACCCTCACCCCGGCCACCTTCACGGc                                   >  5:313902/1‑149 (MQ=255)
                                                                                                           ttattTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCg                                  >  6:105232/1‑149 (MQ=255)
                                                                                                              ttttACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTc                               <  6:79640/149‑1 (MQ=255)
                                                                                                                  aCAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCGCCACCGAAACCACGACGACCGCAACAAGCATCACCACGCGGAGCGCGCCCTTCACCACGGTCTCACGGAGAACGACGCCCAGACCCACGGCGAACTACGCGGCGATCCCCg                           >  2:84999/1‑149 (MQ=255)
                                                                                                                     gATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCt                         >  3:248865/1‑148 (MQ=255)
                                                                                                                       ttGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGaa                      <  7:247703/149‑1 (MQ=255)
                                                                                                                         gtgtTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGAAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGt                    >  2:188626/1‑149 (MQ=255)
                                                                                                                          tgtTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGt                    >  7:211788/1‑148 (MQ=255)
                                                                                                                            ttCGCTGTTCAGCGATGATTACGCATCACCACGTAAACGACGACGACCGGTAGAATCTTCACGACGCGTAGCGCGGACTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTAc                 <  2:351692/149‑1 (MQ=255)
                                                                                                                            ttCGCTGTTCAGCGATGATTACGCATCACCACCGAACCGACGCCGACCGGTAGAATCATCACGCCGCGGAGCGCGGCCTTCACGCCGTTCGCCGCTAAAACGACGACCATCCCCACGGCCACCTTCACGGCGTTCCCCGCGGAAGTTAc                 >  5:128714/1‑149 (MQ=255)
                                                                                                                               gCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGAcc                                                          >  7:32161/1‑105 (MQ=255)
                                                                                                                               gCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGAcc                                                          <  8:32161/105‑1 (MQ=255)
                                                                                                                               gCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCCCGGCAACCTTCACGGCGTTCACCGCTGAAGTTACGAc              >  1:330806/1‑149 (MQ=255)
                                                                                                                               gCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGa               <  8:211788/148‑1 (MQ=255)
                                                                                                                                cTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGAcc             >  4:155488/1‑149 (MQ=255)
                                                                                                                                cTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGAcc             <  6:129108/149‑1 (MQ=255)
                                                                                                                                cTGGTCAGCGATGATTAAGCATCACCACCGTAACGACGACGACCGGTAGTATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGAcc             <  4:155372/149‑1 (MQ=255)
                                                                                                                                       gCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCa      >  4:270935/1‑149 (MQ=255)
                                                                                                                                       gCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCa      <  5:105232/149‑1 (MQ=255)
                                                                                                                                       gCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCa      <  5:195127/149‑1 (MQ=255)
                                                                                                                                           tgatTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTc       >  6:172027/1‑144 (MQ=255)
                                                                                                                                           tgatTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTc       <  5:172027/144‑1 (MQ=255)
                                                                                                                                           tgatTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCCCGGACTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGCCCACCTTAACGGCTTTAAACGCTCAAGTTACCACCGCCTTCacgac  >  2:198971/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCACCACGGCCACCTTCACGGCGTTCACCGCTGAAGTTACGACCGCCTTCACGAC  >  NC_000913/3305829‑3306116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: