mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-3-BOP27-SUCB-END-3_S57_L001_R1_001.good.fq | 351,935 | 50,366,879 | 100.0% | 143.1 bases | 149 bases | 97.4% |
| errors | Plate-3-BOP27-SUCB-END-3_S57_L001_R2_001.good.fq | 351,935 | 50,233,950 | 100.0% | 142.7 bases | 149 bases | 97.1% |
| errors | Plate-3-BOP27-SUCB-END-3_S57_L002_R1_001.good.fq | 329,487 | 47,176,399 | 100.0% | 143.2 bases | 149 bases | 97.5% |
| errors | Plate-3-BOP27-SUCB-END-3_S57_L002_R2_001.good.fq | 329,487 | 47,067,826 | 100.0% | 142.9 bases | 149 bases | 96.9% |
| errors | Plate-3-BOP27-SUCB-END-3_S57_L003_R1_001.good.fq | 340,125 | 48,648,117 | 100.0% | 143.0 bases | 149 bases | 97.3% |
| errors | Plate-3-BOP27-SUCB-END-3_S57_L003_R2_001.good.fq | 340,125 | 48,522,620 | 100.0% | 142.7 bases | 149 bases | 97.0% |
| errors | Plate-3-BOP27-SUCB-END-3_S57_L004_R1_001.good.fq | 348,289 | 49,804,823 | 100.0% | 143.0 bases | 149 bases | 97.5% |
| errors | Plate-3-BOP27-SUCB-END-3_S57_L004_R2_001.good.fq | 348,289 | 49,688,131 | 100.0% | 142.7 bases | 149 bases | 97.0% |
| total | 2,739,672 | 391,508,745 | 100.0% | 142.9 bases | 149 bases | 97.2% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 81.4 | 3.9 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 17999 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 41 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.003 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.77702 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 10:34:59 06 Apr 2022 | 10:35:50 06 Apr 2022 | 51 seconds |
| Read alignment to reference genome | 10:35:50 06 Apr 2022 | 10:41:30 06 Apr 2022 | 5 minutes 40 seconds |
| Preprocessing alignments for candidate junction identification | 10:41:30 06 Apr 2022 | 10:42:05 06 Apr 2022 | 35 seconds |
| Preliminary analysis of coverage distribution | 10:42:05 06 Apr 2022 | 10:43:46 06 Apr 2022 | 1 minute 41 seconds |
| Identifying junction candidates | 10:43:46 06 Apr 2022 | 10:43:54 06 Apr 2022 | 8 seconds |
| Re-alignment to junction candidates | 10:43:54 06 Apr 2022 | 10:45:11 06 Apr 2022 | 1 minute 17 seconds |
| Resolving best read alignments | 10:45:11 06 Apr 2022 | 10:46:00 06 Apr 2022 | 49 seconds |
| Creating BAM files | 10:46:00 06 Apr 2022 | 10:47:32 06 Apr 2022 | 1 minute 32 seconds |
| Tabulating error counts | 10:47:32 06 Apr 2022 | 10:48:08 06 Apr 2022 | 36 seconds |
| Re-calibrating base error rates | 10:48:08 06 Apr 2022 | 10:48:11 06 Apr 2022 | 3 seconds |
| Examining read alignment evidence | 10:48:11 06 Apr 2022 | 11:16:51 06 Apr 2022 | 28 minutes 40 seconds |
| Polymorphism statistics | 11:16:51 06 Apr 2022 | 11:16:52 06 Apr 2022 | 1 second |
| Output | 11:16:52 06 Apr 2022 | 11:17:11 06 Apr 2022 | 19 seconds |
| Total | 42 minutes 12 seconds | ||
